35results about How to "The reaction procedure is simple" patented technology

The invention discloses a nucleic acid detection kit for six respiratory viruses and a use method. The nucleic acid detection kit for the six respiratory viruses comprises six respiratory virus reaction liquid, positive control liquid and negative control liquid, and the six respiratory virus reaction liquid is freeze-dried powder which is pre-sub-packaged into eight connecting tubes, wherein the six respiratory virus reaction liquid A / B tubes contain a plurality of probes with different fluorescence labels, primers, enzyme mixtures, reinforcing agents, freeze-drying protective agents, 10 * buffer, nucleotide mixtures and the like; whether respiratory syncytial virus, respiratory adenovirus, human metapneumovirus, parainfluenza virus type I, parainfluenza virus type II and parainfluenza virus type III exist or not can be detected in the same reaction system, each of the A / B tubes is provided with an internal reference control, and the internal reference control is a human conserved gene beta-actin fragment marked as ROX; and the internal reference control can display the occurrence of false negative, so that the occurrence of a false negative interpretation result caused by an inhibitor in a sample or misoperation is avoided, and the accuracy of PCR detection is improved.

The invention discloses a primer pair composition for identification or assisted identification of H6N1 subtype of avian influenza virus and application of the primer pair composition. The primer pair composition for identification or assisted identification of H6 and / or N1 subtype of avian influenza virus is composed of two primer pairs which are independently packaged, namely a polymerase chain reactor (PCR) primer pair named as H6 and a PCR primer pair named as N1; the H6 is composed of two single-stranded DNA as shown in SEQ ID NO. 1 and SEQ ID NO. 2; and the N1 is composed of two single-stranded DNA as shown in SEQ ID NO. 3 and SEQ ID NO. 4.

The invention is suitable for the field of chemical pharmacy and provides a method for preparing ceftazidime hydrochloride. The method comprises a reaction of carrying out acylation to form ester and a hydrolysis reaction. The method for preparing the ceftazidime hydrochloride, which is disclosed by the invention, greatly simplifies the reaction process an reduces use of raw materials and energy by omitting steps of filtering, extracting, drying and the like after the esterification reaction, so that production cost is greatly reduced; and moreover, the method prevents loss of ceftazidime tert-butyl ester and improves production benefits.

The invention discloses a method for rapidly synthesizing a nanometer 3A molecular sieve. The method is implemented in a way that aluminum hydroxide, sodium hydroxide, potassium hydroxide, potassium silicate and amorphous silicon dioxide are taken as raw materials for synthesizing the nanometer 3A molecular sieve. The method for synthesizing the nanometer 3A molecular sieve comprises the following steps of: dissolving an aluminum hydroxide solid into a mixed solution of sodium hydroxide, potassium hydroxide and potassium silicate; heating and crystalizing while stirring, wherein the alkalinity of the solution is gradually increased along with crystallization; adding aluminum hydroxide and amorphous silicon dioxide for reacting with an alkali; continually crystalizing a product; and filtering and drying a crystallization product to obtain a nanometer 3A molecular sieve product. The method has the characteristics that: (1) the reaction program and process flow are simple, and the synthesis time is short; (2) the raw material utilization ratio is high, and the prepared 3A molecular sieve has low pH value and is not required to be washed; and (3) the prepared nanometer 3A molecular sieve has the advantages of high product purity, large specific surface area, high selective adsorption performance and the like, and has low price.

The invention is applicable to the field of chemical pharmaceutical preparation, and provides a method for preparing cefotaxime through a solid-phase synthetic method. The method comprises the steps of bridging connection of raw material 7-ACA and a solid phase, solid phase synthesis and a separation reaction of a product and the solid phase. The preparation method for the cefotaxime provided by the invention avoids the steps such as post-treatment, purification and drying in the reaction process, greatly simplifies the reaction operation, reduces impurities in the reaction process, and improves the production benefits and the product quality.

In reacting pentose-1-phosphoric acid with a nucleic acid base or a nucleic acid base analogue in an aqueous reaction medium in the presence of a metal cation to produce a nucleoside compound, the timing or method of addition of at least one of these components to the aqueous reaction medium is varied; thereby, a nucleoside compound can be produced at a high yield efficiently without inviting the high viscosity or solidification of the reaction mixture, even when the above components are used in such amounts that the reaction mixture becomes highly viscous or is solidified when the components are used without the above variation of the addition timing or method. Thus, there can be provided a process for producing a nucleoside compound, which comprises a step of reacting pentose-1-phosphoric acid with a nucleic acid base or a nucleic acid base analogue in the presence of nucleoside phosphorylase activity, which gives a nucleoside compound at an improved conversion, and which has wide applicability.

The invention discloses a norovirus universal nucleic acid detection kit and a use method thereof. The norovirus universal nucleic acid detection kit comprises a norovirus reaction solution, a positive control solution and a negative control solution, and the norovirus reaction solution is freeze-dried powder which is pre-packaged into eight connecting tubes, wherein the norovirus reaction solution contains a plurality of probes with different fluorescence labels, primers, an enzyme mixture, an enhancer, a freeze-drying protective additive, a 10 * buffer, a nucleotide mixture and the like, and whether the norovirus is infected or not can be detected in the same reaction system. The whole process of sample collection, nucleic acid extraction, amplification and detection is monitored through the endogenous internal reference, and misjudgment caused by false negative results is avoided. The probes are oligonucleotide comprising a fluorescence reporter group and a quenching group. When the probes are complete, the quenching group is close to the reporter group in spatial position, so that fluorescence emitted by the reporter group is inhibited. When the primers extend, the probe combined with the template is cut off by Taq enzyme (5 '-> 3' exonuclease activity), the reporter group is separated from the quenching group, and a fluorescence signal is generated, so that the detection of the norovirus on the nucleic acid level is realized.

The invention relates to a nucleic acid detection kit for an influenza A virus and an influenza B virus and a use method of the nucleic acid detection kit. The nucleic acid detection kit comprises an influenza A / B virus reaction solution, a positive control solution and a negative control solution, wherein the influenza A / B virus reaction solution contains a plurality of probes with different fluorescence labels, primers, an enzyme mixture, an enhancer, a freeze-drying protective additive, a 10xbuffer, a nucleotide mixture and the like, and can be used for detecting whether the A / B influenza virus is infected or not in the same reaction system. The whole process of sample collection, nucleic acid extraction, amplification and detection is monitored through the endogenous internal reference, and misjudgment caused by false negative results is avoided. When the probe is complete, a quenching group is close to a reporter group in spatial position, so that fluorescence emitted by the reporter group is inhibited. When the primer extends, the probe combined with a template is cut off by Taq enzyme (5 '-> 3' exonuclease activity), the reporter group is separated from the quenching group, and a fluorescence signal is generated, so that the influenza A virus and the influenza B virus are detected on the nucleic acid level.

The invention provides a solid-phase synthesizing method of ceftriaxone sodium. The solid-phase synthesizing method is characterized by including: bridging raw material A to a solid-phase carrier, performing substitution and acylation reactions, and separating from the solid-phase carrier to obtain the ceftriaxone sodium. By using the solid-phase synthesizing method of the ceftriaxone sodium, the post-processing process after the reactions can be omitted, the reaction procedures are simplified greatly, product loss during the post-processing is lowered, the total yield of the reactions is increased to 90% and above, the purity of the ceftriaxone sodium is increased to 99.5% and above, and production benefits are increased.

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming morphological and physiological identification as well as ITS clone sequencing identification method of pathogen with high cost, the invention has the advantages of rapid, accuracy and economy, and simultaneous identifications of a plurality of pathogenic fungus.

The invention discloses a preparation method of 5-{2-(ethylthio) propyl}-3-hydroxy-hexanaphthene-2-ketene. The method belongs to a method generating 5-{2-(ethylthio) propyl}-3-hydroxy-hexanaphthene-2-ketene by using 5-bromine-m-dimethoxy benzene as raw materials through condensation reaction, addition reaction, substitution reaction, catalyzed hydrolysis, catalytic hydrogenation. The method provided by the invention has the advantages that the reaction procedure is simple; the reaction process is mild; the reaction yield is high; particularly, the use of ethanethiol is avoided; the influence on the environment can be effectively reduced; the production and the personnel safety of peripheral staff are ensured.