The invention relates to a construction method of a medical human amniotic membrane tissue reserve
bank, comprising the following steps of: 1, selecting materials of human amniotic membranes and establishing a file; 2, taking a
fetal membrane with a donor meeting medical standard cesarean section or normal labor; separating camisia foetus and maintaining amniotic membranes; 3, repeatedly rinsing the amniotic membranes by using a
phosphate buffering solution and
cutting into membrane sheets; 4, utilizing a
phosphate buffering solution containing
gentamicin with the concentration of 1000 U / ml and
amphotericin B with the concentration of 2.5 micrograms /
millimeter; 5, placing the membrane sheets into a sterile
cell culturing
bottle or
plastic bag of a
phosphate buffering solution containing 4-100% of
glycerol and freezing and storing at a temperature in a range of 20 DEG C below zero to 80 DEG C; 6, carrying out
radiation sterilization treatment on human amniotic membrane sheets by
cobalt 60 to obtain medical human amniotic membrane sheets; and 7, placing amniotic membrane
tissue membrane sheets to be frozen and stored at 80 DEG C below zero to establish an amniotic membrane
tissue membrane sheet file capable of being searched, namely establishing the human amniotic membrane
tissue membrane sheet reserve
bank which has long storage time and can be effectively used for
clinical treatment in time.