125 results about "Fetal membrane" patented technology

A method and a pharmaceutical composition for inhibiting premature rapture of the fetal membranes, ripening of the uterine cervix and preterm labor of female mammals including human. The method includes the step of administering compounds for reversing at least two biochemical conditions being associated with the above processes. The pharmaceutical composition includes compounds for reversing at least two biochemical conditions being associated with the above processes.

The invention discloses a tire fetal-membrane surface character defect detection method based on machine vision, which comprises the following steps: scanning tire fetal membranes to be detected and acquiring a set of images, respectively processing the acquired image and then obtaining the external arc-shaped contour of the tire; fitting circle center and radius of the external arc-shaped contour of the tire, conversing the external arc-shaped image to be detected of the tire to a straight type image to be detected through polar coordinates transformation, performing threshold segmentation, positioning a ROI image to be detected; obtaining the straight type image corresponding to a CAD design image of the tire fetal membrane to be detected, intercepting the straight type image to obtain an image block matched with each ROI image to be detected; performing character identification on each ROI image to be detected and the matched image block, and performing defect determination according to the character identification result. The method can automatically detect the character defect of the tire fetal membrane, has the advantages of high detection stability, low detection cost and fast detection speed, and can be widely used in the tire fetal membrane detection field.

Provided is a pharmaceutical composition for permeabilizing fetal membranes including an active ingredient having a log K in the range of 2 to 4, where K is the octanol / water partition coefficient. The active ingredient may be, for example, bupivacaine, sodium lauryl sulfate or oleic acid. Further provided is a system for transfetal membrane transport. The system includes a probe unit adapted for insertion into a female reproductive tract and releasing a substance onto fetal membranes that permeabilizes the membranes. The system is also configured to apply ultrasound radiation to the fetal membranes to further increase the membrane permeability.

The present invention describes the incorporation of liposomal gene constructs directly into a wound to further improve wound repair, or into wound coverage and / or closure materials to enhance the functionality of the material. The present invention further describes the use of human fetal membranes (e.g., amnion) enhanced with the liposomal gene therapy as a wound coverage material in full-thickness wound repair. The enhanced fetal membranes or enhanced cadaver skin have advantages over currently used materials lacking the liposomal gene construct and are an efficient and safe approach to improve clinical outcome in patients with burn injuries.

The invention provides a feed formula for cows in perinatal period. The feed formula comprises following components in parts by mass: 5-8 parts of traditional Chinese medicine composition, 950-1000 parts of basic concentrated feed, 0.03-0.05 parts of vitamin A, 0.15-0.20 parts of vitamin E, 0.01-0.02 parts of vitamin D, 15-17 parts of rumen protected fatty acid calcium, 3-5 parts of calcium hydrophosphate, 1.0-1.5 parts of sodium bicarbonate, 0.01-0.05 parts of ferrous sulfate, 0.08-0.25 parts of zinc sulfate, 0.5-1.0 parts of magnesium oxide, 9-12 parts of glucose, 3-6 parts of brown sugar, 1-2 parts of white sugar, 1-5 parts of edible salt and 0.1-1.0 parts of vegetable oil. Feeding the cows in the perinatal period with the feed formula according to the method, the incidence of gynaecological diseases such as fetal membrane retaining after delivery, endless lochia, postpartum weakness, postpartum pain, childbed fever, endometritis, metrauxe and vaginitis is obviously reduced, both the oestrus rate once again within 30-40 days after the delivery and conception rate once again within 80 days after the delivery are obviously increased, a target of one delivery per year is basically achieved, the use of antibiotics is decreased to enable the cows and calves to be healthy and obtain good beef quality, the cost for producing the calves is also reduced, and a good economic effect is achieved.

The invention relates to a combined assay kit for premature birth of a pregnant woman and fetal membrane premature rupture, which comprises a premature birth and fetal membrane premature rupture immunochromatographic assay test strip or comprises a premature birth immunochromatographic assay test strip and a fetal membrane premature rupture immunochromatographic assay test strip. More preferably, the combination mat and the nitrocellulose membrane of the premature birth immunochromatographic assay test strip are respectively provided with a premature birth assay immunolabelling antibody and a premature birth assay antibody; the combination mat and the nitrocellulose membrane of the fetal membrane premature rupture immunochromatographic assay test strip are respectively provided with a fetal membrane premature rupture assay immunolabelling antibody and a fetal membrane premature rupture assay antibody, or the immunolabelling antibodies and the antibodies are respectively coated onto the combination mats and the nitrocellulose membranes of the premature birth and fetal membrane premature rupture immunochromatographic assay test strip. The combined assay kit for the premature birth of the pregnant woman and the fetal membrane premature rupture is capable of simultaneously diagnosing the premature birth and the fetal membrane premature rupture through primary sampling, is simple and convenient in application, and further, is capable of improving the accuracy of premature birth diagnosis. A powerful basis is provided for the next accurate treatment by combined assay results. The combined assay kit for the premature birth of the pregnant woman and the fetal membrane premature rupture is suitable for popularization and application in a large scale.

A method is taught for the accurate determination of the premature rupture of membranes (PROM), defined as spontaneous rupture of membranes before the onset of uterine contractions. More specifically, a lateral flow assay strip tests for at least two antigens to greatly limit or eliminate the possibility of false negatives. A built in timer in the cassette holding the lateral flow assay further increases the accuracy of the test. A collection buffer vial with self-contained shipping and dropper caps and built in stand is also taught.

The presence of 3-chlorotyrosine in vaginal secretions in a predictor of the likelihood preterm premature rupture of the fetal membranes, i.e., the chorioamnion and / or the occurrence of the risk of preterm labor. 3-chlorotyrosine is a marker for the excessive production of hypochlorous acid which causes focal areas of increased collagen destruction in the chorioamnion, preterm premature rupture of membranes, or increased production of postaglandin and preterm labor. The inventive method comprises determining the likelihood of the occurrence of preterm premature rupture of membranes or increased production of postaglandin and preterm labor in a pregnant female by obtaining a sample of the females vaginal secretions, and analyzing the secretions for the presence and amount of 3-chlorotyrosine in the sample. Various antibody-based tests can be used to measure 3-chlorotyrosine, and a number of neoantigens, useful in raising antibodies to 3-chlorotyrosine, are disclosed.

The invention relates to a traditional Chinese medicine veterinary drug for treating postpartum retention of fetal membranes of cows and a preparation method of the traditional Chinese medicine veterinary drug. The traditional Chinese medicine veterinary drug is prepared from the following ingredients by weight: 10-20 parts of motherwort, 5-20 parts of dandelion, 5-15 parts of angelica sinensis, 6-9 parts of herba epimedii, 5-9 parts of ligusticum wallichii, 5-7 parts of bighead atractylodes rhizome, 4-8 parts of codonopsis pilosula, 4-8 parts of astragalus membranaceus, 4-8 parts of hawthorn, 4-7 parts of semen cuscutae, 3-8 parts of bunge corydalis herb, 3-5 parts of rhizoma cyperi, 2-5 parts of immature bitter orange, 2-4 parts of peach kernel, 2-4 parts of eucommia ulmoides, 2-3 parts of red flowers, 1-3 parts of lonicera japonica and 1-2 parts of honey-fried licorice root. One or more of pericarpium citri reticulatae, pinellia ternate, trogopterus dung, mangnolia officinalis, rehmannia glutinosa, ophiopogon japonicus, fructus forsythiae and rhizoma zingiberis can be added. The traditional Chinese medicine veterinary drug belongs to a nature pure traditional Chinese medicine preparation, the method for curing postpartum retention of fetal membranes of cows is simple and is easy to operate, the effect is significant, no side effect exists, and no medicine residual exists in milk. The absorption is good through oral administration, and the preparation method has a simple process, is suitable for industrial production, and has the characteristic of simplicity, convenience and fastness in use. Compared with other preparations, the traditional Chinese medicine veterinary drug is more suitable for treatment of postpartum retention of fetal membranes of cows in large-scale livestock farms.

The present invention provides a method for preventing or repairing damage to a fetal membrane. In one embodiment, the method comprises contacting a fetal membrane with a composition comprising a four-armed catechol-terminated polyethylene glycol (cPEG) and a biocompatible oxidant. In one embodiment, the four-armed cPEG and the biocompatible oxidant are initially contained in separate solutions, and the solutions are mixed to form the composition just prior to or at the same time that the composition contacts the fetal membrane.

A Chinese herbal medicine for treating cow retained fetal membranes belongs to dug for animals. According to the formula, the Chinese herbal medicine comprises 55 grams of angelica sinensis, 40 grams of rhizoma ligustici chuanxiong, 40 grams of semen persicae, 80 grams of ginger, 50 grams of radix astragali, 120 grams of herba leonuri, 100 grams of plantago asiatica, 20 grams of liquorice, 30 grams of poria, 30 grams of fructus aurantii and 200 grams of brown sugar. The Chinese herbal medicine is low in cost, treats both symptoms and root causes, has a good treatment effect and high cure rate and is convenient to use.

This invention is about a method for the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic reagent system and diagnostic kit for the diagnosis. The method, diagnostic reagent system, and kit are based on the finding that the level of MMP-8 in the amniotic fluid is significantly higher when the pregnant woman is at risk for preterm delivery, intrauterine infection, and fetal damage. The diagnostic reagent system and kit can be applied to the patients without, as well as with, clinical signs of preterm labor or premature rupture of fetal membranes. With the superiority in sensitivity and specificity as well as its less invasiveness compared to the conventional method of measuring fetal blood cytokine levels, this diagnostic reagent system and kit is very useful in the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage.

A method and a pharmaceutical composition for inhibiting premature rapture of the fetal membranes, ripening of the uterine cervix and preterm labor of female mammals including human. The method includes the step of administering compounds for reversing at least two biochemical conditions being associated with the above processes. The pharmaceutical composition includes compounds for reversing at least two biochemical conditions being associated with the above processes.

The invention provides a system and method for trans-fetal membranes transport. A source of ultrasound sonication is used to deliver ultrasound sonication to fetal membranes to enhance the permeability of the fetal membranes of a gestational sac. In one embodiment, a device is used to collect substances transported from the interior of the gestational sac to the exterior of the gestational sac, such as amniotic or coelomic fluid. In another embodiment a device is used to deliver one or more substances, such as a drug, to an external surface of the fetal membranes.

The invention discloses sodium selenite vitamin injection and a preparation method thereof and belongs to the technology of veterinary medicaments. The vitamin injection is water-soluble injection which is prepared from active ingredients of vitamin A, vitamin D3, vitamin E and sodium selenite through a solubilizer Tween, span, polyoxyethylenated castor oil (35), a preservative 2, and 6-ditertiary butyl paracresol. The sodium selenite vitamin injection has the effects of improving the nutritional conditions of cows, enhancing the cow body physique, accelerating the oestrous period, improving the artificial fertilization and conception rate, preventing hysteritis and retained fetal membranes of the cows, promoting healthy development of calves, and preventing white muscle disease of the calves.

Provided are a method of culturing pluripotent stem cells in the presence of a decidua-derived cell or an extracellular matrix derived from the cell, that enables safe and efficient maintenance culture and derivation of pluripotent stem cells; a culture agent for pluripotent stem cells, that comprises a decidua-derived cell or an extracellular matrix derived from the cell; and other means for developing or performing the method.

The invention discloses an anti-overflow amniotic fluid breaking membrane drainage device for gynecology and obstetrics, belongs to the technical field of medical instruments, and solves the problemsthat the rupture membrane of the existing amniotic fluid rupture membrane device is easy to injure the fetus and the operation safety is poor, and the amniotic fluid overflows. The technical essentials of the invention are as follows: the device comprises an arc-shaped top end, a liquid storage cylinder, a push handle and a holding cylinder, and the liquid storage cylinder and the holding cylinderare connected as one body; the push handle is pushed to make the push rod and the puncture head slide steadily in the direction of the metal drainage tube, the ruptured needle pierces the puncture hole to puncture the fetal membrane, the amniotic fluid is easy to break and safe, and the amniotic fluid can be drained without injuring the fetus; and, secondly, the amniotic fluid is sucked out by the amniotic fluid collecting tube and drainage tube, the puncture needle is automatically retracted under the spring tension, and the annular protrusion and anti-overflow cover slow down and prevent the overflow of amniotic fluid, which is conducive to the drainage operation of amniotic fluid.

In accordance with the present invention, recombinant human PBEF has been produced in order to study its ability to stimulate the transcription of the key cytokine mediators of labor induction, using the amnion-like epithelial (WISH) cell line and fetal membrane explants. As a result of these studies, it has been determined that PBEF is a good indicator of initiation of labor. This makes PBEF a useful marker, especially for the identification of subjects who have initiated preterm labor. Accordingly, methods for the identification of subjects who have commenced labor are provided. In addition, methods are also provided for blocking labor, as well as compositions useful therefor. This is accomplished by blocking the production of PBEF or by blocking the action of PBEF.

Assay methods for identifying an increased risk of preterm delivery induced by premature rupture of the fetal membranes are provided. More specifically, the invention provides salivary protease assays indicative of amniochorion and fetal membrane concentrations of proteases involved in the degradation of the fetal membranes leading to rupture. An increase in salivary protease levels beyond normal or individual baseline provides an indication that protease levels in fetal membranes and / or the amniotic fluid are abnormally elevated. Abnormally elevated protease levels define an increased risk of a premature delivery caused by rupture of the fetal membranes. Thus, abnormally high levels of salivary protease levels indicates elevated risk of PROM, and provides an indication for therapeutic intervention to prevent PROM-induced preterm labor.

The invention discloses a feed additive with motherwort herb extract and a preparation method of the feed additive. The feed additive adopts the motherwort herb extract and a carrier as materials; the motherwort herb extract is prepared by adopting ultrasonic-assisted water extraction method. In the preparation process of the feed additive, the material waste is less, the content of active components in the extract is high, the extract yield is high, and the benefit to improving the feed effect and reducing the production cost is achieved; the prepared feed additive has good adjusting function for endless lochia and retained fetal membranes and the like after delivery of female animals.

The invention discloses a detection kit for premature rupture of fetal membranes, which comprises a detection test paper strip or a test paper card for premature rupture of fetal membranes, a buffer and a cotton swab, the test paper strip or the test paper card comprise a detection zone (T) coated with anti-human insulin-like growth factor binding protein-1 monoclonal antibody 1 and a quality control zone (C) coated with anti-mice polyclonal antibody and colloidal gold coated with anti-human insulin-like growth factor binding protein-1 monoclonal antibody 2. The preparation method of the kit comprises the following steps: preparing a treating liquid, preparing the kit, preparing a colloidal gold compound, spraying metal, cutting, assembling, cutting, packaging and the like. The detection kit for premature rupture of fetal membranes has the advantages of good specificity, high sensitivity, rapid and simple detection, and no pain in a usage process, and can be used for self-test in household and test on bed.

In accordance with the present invention, recombinant human PBEF has been produced in order to study its ability to stimulate the transcription of the key cytokine mediators of labor induction, using the amnion-like epithelial (WISH) cell line and fetal membrane explants. As a result of these studies, it has been determined that PBEF is a good indicator of initiation of labor. This makes PBEF a useful marker, especially for the identification of subjects who have initiated preterm labor. Accordingly, methods for the identification of subjects who have commenced labor are provided. In addition, methods are also provided for blocking labor, as well as compositions useful therefor. This is accomplished by blocking the production of PBEF or by blocking the action of PBEF.

The invention provides a system and method for trans-fetal membranes transport. A source of ultrasound sonication is used to deliver ultrasound sonication to fetal membranes to enhance the permeability of the fetal membranes of a gestational sac. In one embodiment, a device is used to collect substances transported from the interior of the gestational sac to the exterior of the gestational sac, such as amniotic or coelomic fluid. In another embodiment a device is used to deliver one or more substances, such as a drug, to an external surface of the fetal membranes.

The invention discloses a posture pad for premature rupture of fetal membranes. The posture pad comprises a latex pad conforming to the human body structure and a waterproof membrane, wherein the waterproof membrane is disposed on the upper end face of the latex pad and matched with the latex pad for use; multiple through holes are formed in the middle of the latex pad, multiple liquid discharge pipes corresponding to the through holes one to one are arranged on the lower end face of the waterproof membrane and inserted into and penetrate through the through holes respectively; a supporting plate is provided on the lower end face of the latex pad, a through groove corresponding to the through holes is formed in the middle of the supporting plate, and a liquid collecting bracket is arrangedon the portion, located at the through groove, of the bottom of the supporting plate; a liquid collecting box is arranged in the liquid collecting bracket, a first connecting rod is rotatably connected to the lower plane of the supporting plate, and a supporting rod is disposed at the end, away from the supporting plate, of the first connecting rod; the middle of the first connecting rod is rotatably connected to a transmission block through a second connecting rod, the transmission block is disposed on a screw rod, the end of the screw rod is provided with a handle capable of driving the screw rod to rotate, and ascending and descending of the latex pad can be achieved by rotating the screw rod. The posture pad for premature rupture of the fetal membranes is not only simple in structurebut also low in processing cost, safe and hygienic.

The invention provides a method for isolated culture of mesenchymal stem cells from fetal membranes. The method comprises the following steps: tissue separation, cleaning, culture, passage and cryopreservation. The invention provides a method for separating the mesenchymal stem cells, which are derived from an amniotic membrane, chorion laeve and decidua parietalis, from the fetal membranes. The conventional material positions are changed, and the separation process is simplified. The mesenchymal stem cells are extracted with a tissue block wall adherence method, the extraction method is simple and fast, introduction of an exogenous enzyme is not needed, so that risks of an exogenous substance for affecting quality of a cell preparation are reduced, and the prepared cells have high activity and purity and better proliferation capacity.

The invention discloses an invigorating uterus-clearing mixture and a preparation process thereof, and the invigorating uterus-clearing mixture belongs to a traditional Chinese medicine oral liquid for livestock. The invigorating uterus-clearing mixture comprises radix codonopsis , milkvetch root, Chinese angelica, rhizoma chuanxiong, peach kernel, flos carthami, rhizoma zingiberis preparata, honey-fried licorice root, leonurus heterophyllus, radix paeoniae alba, radix bupleuri, sparganii rhizoma, hawthorn, dandelion and the like. The prepared invigorating uterus-clearing mixture is the traditional Chinese medicine oral liquid special for treating insufficiency of vital energy and blood, retained fetal membranes, prolonged lochia, and abdominal pain due to blood stasis of postpartum livestock. The prepared invigorating and uterus-clearing mixture has the functions of supplementing qi and nourishing blood, activating blood circulation and removing blood stasis, and antisepsis and anti-inflammation, has the advantages of quick absorption, quick effect, safety and no drug residues, and is especially suitable for health care and treatment of postpartum dairy cows.

The invention discloses a traditional Chinese medicine for treating postpartum retained fetal membranes of cows. The traditional Chinese medicine is characterized in that a preparation method comprises the following steps: weighing the following medicinal raw materials in parts by weight: 40-50 parts of motherwort herb, 35-40 parts of Chinese angelica, 35-45 parts of prepared rhizome of adhesive rehmannia, 35-40 parts of white paeony root, 25-30 parts of peach seed, 15-20 parts of safflower, 20-30 parts of szechuan lovage rhizome, 25-35 parts of codonopsis pilosula and 20-30 parts of honey-fried liquorice root; uniformly mixing, crushing by using a crushing machine to obtain medicine powder of which the particle size is 50-100mu m, mixing the medicine powder with boiling water according to a mass ratio of (1:3.5) to (1:4.5) to obtain liquid medicine, cooling to 35-40 DEG C, then weighing yellow wine according to the mass ratio of the medicine powder to the yellow wine being (1:0.8) to (1:1.2), adding the yellow wine into the liquid medicine, and uniformly mixing and stirring to obtain a medicament. The traditional Chinese medicine disclosed by the invention is low in cost and wide in material source, is a pure traditional Chinese medicine combination agent, does not have medicine residues, is significant in curative effect, is safer and more efficient when used for treating postpartum retained fetal membranes of the cows according to a conservative treatment method mainly based on traditional Chinese medicines, ensures that the treatment effect is better than that of a conventional surgical dissection method, has the characteristics of complete fetal membrane discharge, high cow recovery speed and no sequelae, and ensures that the effective rate for postpartum retained fetal membranes of the cows can reach 90% and the cure rate can reach 80%.

This invention is about a method for the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic reagent system and diagnostic kit for the diagnosis. The method, diagnostic reagent system, and kit are based on the finding that the level of MMP-8 in the amniotic fluid is significantly higher when the pregnant woman is at risk for preterm delivery, intrauterine infection, and fetal damage. The diagnostic reagent system and kit can be applied to patients with or without clinical signs of preterm labor or premature rupture of fetal membranes. With its superiority in sensitivity and specificity as well as its less invasiveness compared to the conventional method of measuring fetal blood cytokine levels, this diagnostic reagent system and kit is very useful in the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage.

The invention provides a method for differentiating placental wall decidual mesenchymal stem cells into endometrial epithelial cells, which comprises the following steps of: adding a tissue digestivejuice into placental wall decidual tissues for digestion, filtering to remove undigested tissue blocks after the digestion is terminated, collecting cell suspension, centrifuging, removing the supernatant, cleaning the precipitate and centrifuging again to obtain mesenchymal stem cells of placenta wall fetal membrane tissues; culturing the placental wall decidual mesenchymal stem cells by using aselective culture medium of the placental wall decidual mesenchymal stem cells; passing the primary cells of the placental wall decidual mesenchymal stem cells to P3 generation; taking the P3 generation placental wall decidual mesenchymal stem cells, adding the placental wall decidual mesenchymal stem cells into a selective culture medium, and inoculating and culturing overnight; removing the selective culture medium of the placental wall decidual mesenchymal stem cells, and carrying out induction culture by using the endometrial epithelial cell differentiation culture medium to obtain the endometrial epithelial cells. The method can successfully obtain endometrial epithelial cells with cell activity.

The invention provides a Chinese medicine for treating postpartum lochiorrhea and a preparation method thereof. The Chinese medicine comprises the following raw medicinal materials: verbena, motherwort, angelica rhizome, ligustici wallichii, red paeony root, sappanwood, ginseng, dragon's blood, rhizoma sparganii, curcuma zedoary, fried fruit of immature citron, artemisia anomala, herba lycopi, the root of fangji, ground beeltle, caulis spatholobi, the root of red-rooted salvia, endothelium corneum gigeriae galli, radix curcumae, elecampane, rhizoma corydalis, myrrh, resina toxicodendri and radix rubiae. The Chinese medicine provided by the invention has the advantages of fast acting, high function, short treatment course, high safety, no significant toxic and side effects and easy medication, can improve microcirculation and enhance the contraction of uterine smooth muscle, is beneficial for updating of endometrium and inflammation subsiding, and also can drive to exhaust the placenta and fetal membrane remained in uterus automatically so as to remove intrauterine stasis.