53 results about "Premature rupture of membranes" patented technology

A method is taught for the accurate determination of the premature rupture of membranes (PROM), defined as spontaneous rupture of membranes before the onset of uterine contractions. More specifically, a lateral flow assay strip tests for at least two antigens to greatly limit or eliminate the possibility of false negatives. A built in timer in the cassette holding the lateral flow assay further increases the accuracy of the test. A collection buffer vial with self-contained shipping and dropper caps and built in stand is also taught.

The invention discloses a test strip for fast detecting premature rupture of fetal membranes, which is formed in a way that a sample pad, a glass fiber membrane, a coating membrane and absorbent paper are sequentially overlapped and pasted on a substrate, wherein the glass fiber membrane is coated with a high-specificity monoclonal antibody of latex particle-labeled insulin-like growth factor binding protein-1 (IGFBP-1); and the coating membrane comprises a detection area coating an anti-IGFBP-1 specific monoclonal antibody and a control area coating an anti-mouse antibody. Compared with the radioimmunoassay method and the enzyme-linked immunosorbent assay (ELISA) method, the test strip disclosed by the invention has the advantages of operational safety, fast detection and the like, is simple and convenient and is applicable to individual detection. The test strip disclosed by the invention can be conveniently and fast operated by people without professional skills, can not cause damages and can realize timely on-site and household self detection of premature rupture of fetal membranes; and the result can be read easily.

The invention provides an air cushion used for lifting buttocks of a pregnant woman for treatment during premature rupture of the membrane of the pregnant woman. The air cushion comprises an upper part and a lower part, wherein the upper part is a longitudinal rectangle from an overlook view and is a triangle from top down from a lateral view, and an air inflation hole, an air inflation tube, an air inflation piston and an air inflation ball are arranged on a side surface; and the lower part is a transverse rectangle from the overlook view and is a rectangle slightly higher than the bottom edge of the triangle from the lateral view, and an air inflation hole, an air inflation tube, an air inflation piston and an air inflation ball are arranged on a side surface. The air cushion solves the problems that at present, when premature rupture of the membrane occurs on the pregnant woman, the amniotic fluid flows out, the buttocks are in urgent need of being lifted for treatment, while the traditional method which adopts toilet paper to support below the buttocks is unreliable in effect, inconvenient to use, unsafe and uncomfortable. The air cushion which is lifted gradually from top down can relieve the discomfort in the waist part, the back part and the shoulder part caused by lifting of the buttocks, can be folded, packaged and placed in a carried bag in a non-inflation state for emergency, and is portable. During premature rupture of the membrane of the pregnant woman, the pregnant woman lies the buttocks lifting air cushion, so that complications such as oligohydramnios, prolapse of umbilical cord, fetal distress, and even intrauterine death and the like caused by outflow of the amniotic fluid can be prevented or reduced, and the buttocks lifting operation for treatment is simple, convenient and safe.

Assay methods for identifying an increased risk of preterm delivery induced by premature rupture of the fetal membranes are provided. More specifically, the invention provides salivary protease assays indicative of amniochorion and fetal membrane concentrations of proteases involved in the degradation of the fetal membranes leading to rupture. An increase in salivary protease levels beyond normal or individual baseline provides an indication that protease levels in fetal membranes and/or the amniotic fluid are abnormally elevated. Abnormally elevated protease levels define an increased risk of a premature delivery caused by rupture of the fetal membranes. Thus, abnormally high levels of salivary protease levels indicates elevated risk of PROM, and provides an indication for therapeutic intervention to prevent PROM-induced preterm labor.

The invention discloses a detection kit for premature rupture of fetal membranes, which comprises a detection test paper strip or a test paper card for premature rupture of fetal membranes, a buffer and a cotton swab, the test paper strip or the test paper card comprise a detection zone (T) coated with anti-human insulin-like growth factor binding protein-1 monoclonal antibody 1 and a quality control zone (C) coated with anti-mice polyclonal antibody and colloidal gold coated with anti-human insulin-like growth factor binding protein-1 monoclonal antibody 2. The preparation method of the kit comprises the following steps: preparing a treating liquid, preparing the kit, preparing a colloidal gold compound, spraying metal, cutting, assembling, cutting, packaging and the like. The detection kit for premature rupture of fetal membranes has the advantages of good specificity, high sensitivity, rapid and simple detection, and no pain in a usage process, and can be used for self-test in household and test on bed.

The invention belongs to the field of medical health care technology, and particularly provides a screening method for difference protein of vaginal secretion in pregnant woman with premature ruptureof membranes based on iTRAQ technology. By combining the iTRAQ technology and the 2DLC-MS-MS technology, a rapid and high correlation degree comprehensively screening of the vaginal secretion difference protein of the premature woman with premature rupture of membranes is achieved, after further identification analysis, the screened differential proteins IGFBP-1, AFP, PRL and LZM can be used as biomarkers for diagnosis of premature rupture of membranes with higher sensitivity and specificity.

The invention discloses application of a regent for detecting Axl receptor tyrosine kinase to prepare a kit for detecting whether a pregnant woman has a premature rupture of membranes. The regent is a monoclonal antibody of the Axl receptor tyrosine kinase which is labeled by biotin. The kit includes a perforated plate coated with the monoclonal antibody of the Axl receptor tyrosine kinase, biotin-labeled monoclonal antibody detection liquid of the Axl receptor tyrosine kinase, avidin-horse radish peroxidase combined with biotin-labeled monoclonal antibody of the Axl receptor tyrosine kinase, a chromogenic substrate 3',3',5,5'-tetramethylbenzidine, and an Axl protein standard substance. A preparation method for the kit comprises the following steps of: (1) preparing the perforated plate coated with the monoclonal antibody of the Axl receptor tyrosine kinase; (2) preparing the biotin-labeled monoclonal antibody detection liquid of the Axl receptor tyrosine kinase; and (3) preparing avidin-horse radish peroxidase, the chromogenic substrate 3',3',5,5'-tetramethylbenzidine, and the protein standard substance of the Axl receptor tyrosine kinase.

The invention provides a monoclonal antibody of human insulin-like growth factor binding protein-1, a monoclonal antibody host cell, a diagnostic reagent with the monoclonal antibody of the human insulin-like growth factor binding protein-1and a diagnostic reagent kit, wherein antigen of the monoclonal antibody is antigenic determinant of the human insulin-like growth factor binding protein-1; preferably, the antigenic determinant comprises an amino acid sequence represented by SEQIDNO:1; and the monoclonal antibody host cell contains the monoclonal antibody of the human insulin-like growth factor binding protein-1. The monoclonal antibody of the human insulin-like growth factor binding protein-1 can perform specific detection on the human insulin-like growth factor binding protein-1, and the prepared diagnostic reagent has high specificity of premature rupture of membrane diagnosis, rapid reaction, low cost and suitability for large-scale popularization and application.

This invention is about a method for the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage, and diagnostic reagent system and diagnostic kit for the diagnosis. The method, diagnostic reagent system, and kit are based on the finding that the level of MMP-8 in the amniotic fluid is significantly higher when the pregnant woman is at risk for preterm delivery, intrauterine infection, and fetal damage. The diagnostic reagent system and kit can be applied to patients with or without clinical signs of preterm labor or premature rupture of fetal membranes. With its superiority in sensitivity and specificity as well as its less invasiveness compared to the conventional method of measuring fetal blood cytokine levels, this diagnostic reagent system and kit is very useful in the prenatal diagnosis of preterm delivery, fetal infection, and fetal damage.

The invention discloses a detection kit and a preparation method thereof, specifically elates to a kit for detecting premature rupture of membrane and a preparation method thereof. The kit comprises a multi-cell plate and reagents, wherein the reagents comprise: A, a magnetic microsphere suspension, wherein the magnetic microspheres are prepared by co-cross-linking hydroxylation magnetic microspheres with a diameter of 0.5-2 mum, N-hydroxysulfo succinic acid amide and carbodiimide, and a concentration of the magnetic microspheres in the suspension is 75-85/muL; B, phycoerythrin-labeled streptavidin and a biotin-labeled antibody detection solution, wherein the biotin is N-hydroxy succinic acid imino ester, the antibody is mouse anti-human soluble intercellular adhesion molecule-1 monoclonal antibody, and a concentration of the biotin-labeled antibody is 1.7-2 mg/mL; and C, a soluble intercellular adhesion molecule-1 protein standard substance and a standard substance dilution solution. The kit provides significantly increased sensitivity and specificity for the detection result of premature rupture of membrane so as to reduce diagnostic error incidence rate, wherein the sensitivity and the specificity can respectively achieve more than 99% and 98%.

The present invention provides compositions and methods for use in treatment of premature rupture of membranes. The compositions of the invention include a therapeutic amount of an avian thick egg white composition. Preferably, the compositions include the purified thick egg white protein, ovomucin. The methods of the invention involve injecting an effective amount of the compositions into the prematurely ruptured amniotic sac of a patient in order to seal the rupture.

A method is taught for the accurate determination of the premature rupture of membranes (PROM), defined as spontaneous rupture of membranes before the onset of uterine contractions. More specifically, a lateral flow assay strip tests for at least two antigens to greatly limit or eliminate the possibility of false negatives. A built in timer in the cassette holding the lateral flow assay further increases the accuracy of the test. A collection buffer vial with self-contained shipping and dropper caps and built in stand is also taught.

The invention discloses a biomarker and a method for predicting premature rupture of fetal membranes, and the biomarker is selected from one or more of 4-methylvaleraldehyde (isohexanal), sphingolipids (SP) metabolite, glycerolipids (GL) metabolite and glycerophospholipids (GP) metabolite; the biomarker has a change in content difference multiple between a pregnant female body and a healthy pregnant female body at risk of premature rupture of fetal membranes. The biomarker for predicting premature rupture of fetal membranes and the prediction method have important significance for early prediction of premature rupture of fetal membranes before delivery and formulation of corresponding intervention measures.

The invention relates to an aptamer capable of being specifically combined with ICAM-1. The aptamer has the high combining characteristic and the high stability. The aptamer is obtained in the mode that the new combinatorial chemistry technology SELEX is applied, ICAM-1 serves as a target, and the DNA aptamer capable of being specifically combined with the ICAM-1 is screened out from a random library. The invention further relates to a kit for identifying the premature rupture of fetal membranes, and the kit can rapidly identify the premature rupture of fetal membranes, and has quite high application value.