196 results about "Citrate buffer" patented technology

It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230° C. for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of β-glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42° C.

A stable liquid medical formulation containing an antibody is provided, which contains a therapeutically effective amount of an antibody in a glutamate buffer and / or a citrate buffer and has a pH between 4.0 and 6.0.

The invention discloses washing liquid used in the semiconductor industry to wash plasma etching residues. The washing liquid contains citric acid / citrate buffer solution, fluorides, macromoledular corrosion inhibitor, anti-freeze agent and solvent. The washing liquid can effectively wash the plasma etching residues during the process of manufacturing a semiconductor, have low etching velocity on nonmetals and metal substrates such as Si, SiO2, tetraethoxy silane silicon dioxides (PETEOS), low-medium materials, Ti, Al, Cu and so on, is safe and harmless to the environment and the human body, and has good application prospect in the microelectronic field such as washing of semiconductor wafers and so on.

It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230 DEG C for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of beta -glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42 DEG C.

The invention discloses a method for pretreating lignocellulose by using a renewable ionic liquid aqueous solution, including the steps of: (1) taking a choline and amino acid ionic liquid aqueous solution as a pretreatment solvent, mixing lignocellulose and the pretreatment solvent under the protection of nitrogen, stirring at 50-120 DEG C, cooling to room temperature, filtering, washing residue, and drying to obtain the pretreated lignocellulose; and (2) weighing the pretreated lignocelluloses, adding a citrate buffer, adding cellulase, reacting for 3-12 h with 150-250 r / min at 40-60 DEG C to obtain sugar liquid mainly including glucose and xylose. The pretreatment process not only can effectively enhance the efficiency of enzymatic hydrolysis of lignocelluloses, and improve the yield of fermentable reducing sugar, but also has the advantages of environmental protection, renewability, low viscosity, easy operation, low cost, and low power consumption.

The method relates to the method to extract the sunflower oil and recover the protein by water-enzyme method. The producing procedure comprises the following steps: dry-pulverizing the uncoated sunflower seed, adding citrate buffer to the powder and decomposing the powder by compound cellulose; centrifugalizing to get free oil, emulsion, digest and tablet; high speed centrifugalizing to get some free oil; filtering, vacuum concentrating, alcohol precipitating, centrifugalizing and freeze-drying to recycle the protein. The special treatment in the invention has substantially increased the free oil yield and the quality of the free oil is better than the refined oil. The water-extracting and alcohol-precipitating method to get the protein is simple and efficient; besides, the method has maintained the sunflower protein's nutritive value and functional value very well. Compared with the traditional squeezing and water-extracting technologies, the invention has many advantages such as simple producing equipments, safe operation, less energy expenditure and less pollution.

The present invention relates to stable pharmaceutical liquid formulations of the fusion protein TNFR:Fc comprising different buffer systems and stabilizers. In particular, it could be demonstrated that the physical stability of TNFR:Fc is significantly improved by using a citrate buffer system and lysine as stabilizer.

The invention discloses a saccharifying method of xylon cellulose through ultrasonic coordinated modified cellulose enzyme, which comprises the following steps: grinding and predisposoing xylon in the raw material through alkaline; reacting activated methoxy carbowax and cellulose enzyme in the citrate-sodium citrate buffer solution to obtain modified cellulose enzyme; blending modified cellulose enzyme, beta-glucosidase, amylase and pectase to obtain composite liquid; adding composite enzyme into predisposed raw material according to corresponding proportion; proceeding enzyme catalytic reaction through ultrasound; filtering; decompressing; evaporating; obtaining condensed sugar liquid.

The invention discloses a rapid detection method of forbidden azo dyes in dyed textiles, which uses the following reagents: 1) an extracting reagent A: 10 mL of methyl ten-butyl ether; 2) an extracting reagent B: 5 mL of 0.1-1.0 mol / L hydrochloric acid solution; 3) a reagent 1: 0.05 mL of 50 g / L NaNO2 solution; 4) a reagent 2: 0.5 mL of 25 g / L NH4SO3NH2 solution; 5) a reagent 3: 1 mL of o-metoxyphenol ethanol aqueous solution with volume percentage concentration of 2%; 6) a reagent 4: 1 mL of 0.5 mol / L NaOH solution; 7) a reducing agent 1: 16 mL of 0.06 mol / L citrate buffering solution with pH of 6.0; and 8) a reducing agent 2: 3.0 mL of 200 mg / L sodium hydrosulfite solution. According to the invention, the pre-treatment is realized by liquid-liquid extraction of textile reduction solution and discoloring, and the aromatic amine is measured by coloration; the pre-treatment is simple and rapid enough to greatly simplify the original steps, and no instrument or equipment is necessary in the measurement so as to realize the quick screening detection of forbidden azo dyes in textiles.

The invention provides a stable solution formulation comprising a therapeutically effective amount of a GLP-1-Fc fusion protein at about pH 6.5 in citrate buffer with polysorbate-80 and mannitol. The formulation is useful in treating diabetes and obesity as well as a variety of other conditions or disorders.

A method and formulation for preparing spray dried vancomycin. In various embodiment, the formulation includes vancomycin HCl (10-20%) and one or more of the following PEG (0-5%), mannitol (0-5%), ethanol (0-10%), and a citrate buffer. Spray dried vancomycin has favorable reconstitution times and water content.

The invention belongs to the technical field of in-vitro immunodetection and in particular relates to a kit for detecting myeloperoxidase (MPO) concentration in a sample and a preparation method of the kit. The kit comprises a porous plate coated with an anti-MPO monoclonal antibody, an MPO series standard substance and a quality control material, a horse radish peroxidase-labeled anti-MPO monoclonal antibody, a sample buffer solution, a citrate buffer solution of hydrogen peroxide, a citrate buffer solution of tetramethyl benzidine, a 1-2M sulfuric acid solution, and a phosphate buffer solution containing surfactants. Because the used detection instruments are simple and are universal instruments, the kit is low in detection cost and contributes to popularization; the kit is easy to operate and can be realized without professionals. Meanwhile, according to the unique standard diluent and sample buffer solution formula in the kit, the reliability and the accuracy of the detection result are greatly improved.

The invention discloses a fabrication method of resistant rich starch, comprising the following steps: (1) put the raw rice starch into a acid-resistant container and add hydrochloric acid solution with concentration rate of 0.4 mol / L to 0.6 mol / L with the solid-solution weight ratio of 1:3 to 1:5. (2) expose the starch to acidolysis under the temperature of 30 to 40 degrees celcius for 2 to 4 hours and then adjust the PH value of the solution to neutral.(3) add in citrate buffer solution until the PH value of the acid hydrolyzate is adjusted to 5 to 6, add 2 to 4 PUN promozyme to every gram of dry starch, conduct constant temperature bath to the solution to facilitate the process of the enzyme, and then deactivate the enzyme by boiling water bath for 1 to 3 hours. (4) cool down the solution to room temperature and centrifuge the solution before refrigerate it in the fridge at 3-4 degree celcius for 22-24 hours, take the solution out and store 5 to 6.5 hours in room temperature before drying it up in the oven at 75 to 85 degrees, grind it into powder. By employment of the fabrication method of resistant rice starch, the yielding rate is greater or equal to 20 weight percent.

The present invention relates to stable pharmaceutical liquid formulations of the fusion protein TNFR:Fc comprising different buffer systems and stabilizers. In particular, it could be demonstrated that the physical stability of TNFR:Fc is significantly improved by using a citrate buffer system and lysine as stabilizer.

The invention discloses a treatment liquid for desorbing antigens in an aluminum salt adsorption type vaccine. The treatment liquid is a phosphate buffer solution or a citrate buffer solution, wherein the buffer solution contains proteins and at least one acid and / or salts of the acids. The invention further discloses a method using the provided treatment liquid to measure the antigen content of an aluminum salt adsorption type vaccine. The provided method reduces the interference brought by the aluminum adjuvant in Japanese encephalitis vaccine, is capable of rapidly and precisely measuring the antigen content in an adsorption type Japanese encephalitis inactivated vaccine, has the characteristics of good durability, high accuracy, and high precision, and can provide references for quality control of aluminum adjuvant adsorption type Japanese encephalitis inactivated vaccines.

A liquid preparation containing thymopeptide-5 is disclosed. Its preparing process features that the buffering liquid of citrate is used to regulate pH=6.5-7.5, the sodium chloride is chosen as the optimal osmolarity regulator, and the stabilizer and protector are also used.

The invention relates to a chitosan plural gel foaming agent suitable for a female sperm shielding and killing dual-contraception effect and a preparation method thereof, and belongs to the technical field of foaming agent production. The chitosan plural gel foaming agent suitable for the female sperm shielding and killing dual-contraception effect has a dual-contraception effect of vagina shielding and vagina sperm killing, and meanwhile has a fungal-infection-resistant effect. The foaming agent forms a medicament liquid by consisting of a matrix, a surface active agent, an auxiliary medicament, a cosolvent, an acidifier, a foaming agent, a foam stabilizing agent and a solvent, and the foaming agent and a propellent form a plural gel foaming agent according to the weight proportion of (100:10) to 20. The preparation method comprises the following steps: dissolving chitosan and carbomer into a citrate buffer solution with a certain amount; blending, heating to 80 DEG C to dissolve completely, adding the surface active agent so as to obtain a solution A; dissolving borneol, menthol crystal and stearic acid into glycerol, dissolving fully, and filtering so as to obtain a solution B; adding the solution B into the solution A, mixing fully, adding citric acid and SLS (sodium lauryl sulfate), supplying the buffer solution to the required weight, carrying out vacuum homogenizing for 2 hours, thereby obtaining the gel foaming preparation, and then adding the propellent.

The invention provides a binding solution, kit and method for purifying nucleic acid by magnetic bead method as well as application. The binding solution comprises a citrate buffer solution, EDTA, sodium acetate, lithium chloride and isopropyl alcohol but does not comprise guanidine salt. The binding solution contains no guanidine salt, can coexist with magnetic beads for a long time and can fullypromote precipitation of nucleic acid and binding with magnetic beads, the low isopropyl alcohol content contributes to increase of the nucleic acid purity, and nonspecific binding of impurities withmagnetic beads is reduced.

The present invention refers to ready-to-use highly stable paracetamol injectable solutions, prepared by mixing paracetamol, wter, propylene glycol, and a citrate buffer. (pH 4.5 to 6.5), and by heating said solution under preset conditions. The resulting solution may be stored for an extended period of time within a wide range of temperatures, with no paracetamol precipitation and / or its chemical modification.

The invention relates to a method of ionic liquid cosolvent effect reinforced enzymatic synthesis of isoquercitrin. The method comprises a reaction system for synthesizing isoquercitrin by hydrolyzing rutin with a hesperidin enzyme or a naringin enzyme. The reaction system comprises a rutin mother liquid prepared by a disodium hydrogen phosphate-citrate buffer solution and a hesperidin enzyme liquid or a naringin enzyme liquid. The reaction system contains an ionic liquid formed by cations and anions. In the ionic liquid cosolvent system, the prepared isoquercitrin by hydrolyzing rutin under enzyme catalysis has increased reaction rate and higher yield than that in a conventional aqueous solution system, so that the ionic liquid cosolvent system provides a novel reaction medium for the production of isoquercitrin. The method is simple in operation and mild in reaction conditions, is environment-friendly, and has good application prospects.

It is provided a pharmaceutical composition stably containing ghrelin or its derivative, which is an endogenous growth hormone secretagogue (GHS) to a growth hormone secretagogue-receptor (GHS-R), comprising a aqueous solution containing the ghrelins having pH range of 2 to 7, wherein the aqueous solution having pH range of 2 to 7 is a buffer solution, especially, glycine hydrochloride buffer, acetate buffer, citrate buffer, lactate buffer, phosphate buffer, citric acid-phosphate buffer, phosphate-acetate-borate buffer or phthalate buffer, and the concentration of the ghrelins in the solution is from 0.03 nmol / mL to 61 μmol / mL.

The invention discloses a method for solid fermentation to produce tannins by utilization of gallnut uncooked materials, wherein, mixture of gallnut powder and bran with a weight ratio between 5:95 and 35:65 is taken as a culture medium; inorganic salt solution the weight of which is 0.6 to 2.1 times of that of the mixture is added into the mixture and uniformly mixed; the uncooked materials are directly inoculated into aspergillus niger spore suspension; after stand culture for 28 to 168 hours at the temperature of 28 to 35 DEG C, and tannin crude enzymes are obtained through extraction by utilization of citrate buffer. The method takes the gallnut powder and the bran as raw materials, wherein, the gallnut powder is an inducer for production of the tannins. The method adopts the uncooked materials to be directly inoculated into aspergillus niger solids for fermentation production of the tannins without high-pressure steam sterilization treatment according to the characteristic that Chinese specialty gallnuts are easy to be burned through high-pressure steam sterilization treatment. The method avoids damage of high temperature on the raw materials, simplifies equipment and the production flow and improves the production efficiency.

The invention provides a preparation method of a sulfated silk fibroin material. The method comprises the steps of: a, preparing an azo salt stock solution; b, dropping ice-bath cooled sodium nitrite aqueous solution to a mixed solution in the step a, and carrying out ice-bath uniformly to obtain the azo salt stock solution; and c, dissolving silk fibroin in a citrate buffer solution to obtain a silk fibroin solution, adding the azo salt stock solution, maintaining the pH of the citrate buffer solution within 7.5-10, carrying out ice-bath reaction, and dialyzing, freezing and drying to obtain the sulfated silk fibroin. The product prepared by the invention is not only a material with good anticoagulant performance, but also has less immunogenicity, so that the material is a bioactive material which can improve conglutination, diffusion and proliferation capacity of the silk fibroin, cells and growth factors. Types of biomaterials are enriched, and grounds for tissue engineering application such as vessels, ligaments and bones are provided.

A high-stability injection of Paluonosiqiong is prepared from Paluonosiqiong or its medicinal salt, and the additive for injection chosen from citrate buffer solution, xylitol, sorbitol and sodium chloride. It has low cost.

The invention discloses a kit for detecting vibrio parahaemolyticus on the basis of immunomagnetic beads and MnO2 nanometer particles. Magnetic beads with superparamagnetism are prepared; the magnetic beads are coupled with vibrio parahaemolyticus polyclonal antibodies; MnO2 nanometer particles are synthesized; the nanometer particles are coupled with vibrio parahaemolyticus specific chicken egg-yolk antibodies; liquid to be tested is taken and is mixed with two kinds of probes; a magnetic force frame is used for separating magnetic beads-thallus-MnO2 compounds; a citrate buffer solution is used for resuspending the mixture; TMB is added for color development, so that the fast specific detection of the vibrio parahaemolyticus is realized. The vibrio parahaemolyticus detection by using the immunomagnetic beads and MnO2 nanometer particle technology is provided by the invention; the immunological reaction of an ELISA method is used for color development; the detection time is shortened; during the quantitative detection, the variation coefficient is small; the lowest detection concentration is 10 CFU / mL; the adding standard recovery rate reaches 96.7 percent; the sensitivity is high; the stability is high.

The invention provides a method for detecting lead ions based on combination of fluorescence quantitative PCR (polymerase chain reaction) and nuclease GR-5. The method comprises steps of (1) immobilizing nuclease GR-5 and complementary DNA (deoxyribonucleic acid) onto the inner surface of a PCR reaction vessel; (2) adding a sample to be tested into the PCR reaction vessel obtained in step (1), wherein when lead ions exist, in the presence of the nuclease GR-5, the complementary DNA breaks at the ribonucleotide so as to generate a short nucleic acid chain; (3) washing the PCR reaction vessel with citrate buffer solution so as to remove the nucleic acid molecules which are not immobilized; (4) adding an amplification primer into the PCR reaction vessel so as to carry out real-time fluorescence quantitative PCR on the immobilized complementary DNA; (5) confirming the concentration of the lead ions in the sample based on the Ct value of the real-time fluorescence quantitative PCR. According to the method, a brand-new biosensor with high selectivity and high sensitivity is provided and can be used for simply and rapidly detecting the concentration of lead ions in the sample.

This invention relates to one ABO blood reverse shape agent, which is based on the a's liquid and adopts the improved buffer system, that is from single citrate buffer system into citrate and phosphate double buffer system to better keep the system PH value stable, wherein, the invention adds gland purine as nutrient to make the erythrocyte keep bright color during abundant oxygen process; the anti-erosion system not adopting any chemical antigen to avoid impacting the oxygen content to make the red cell dark; adding EDTA sodium salt to prevent blood-stop cell hemolytic phenomenon.

A counter typing reagent of ABO blood type is prepared as improving buffer system to change single citrate buffer system to be citrate-phosphate double-buffer system, adding adenine as nutrition composition, applying no any chemical anticorrosive in anticorrosion system and adding EDTA for preventing red blood cell of blood type from hemolysis.

The invention discloses a proficiency testing sample for pH determination of an aqueous extract of a textile, and a preparation method of the proficiency testing sample. The proficiency testing sample is a piece of impregnation liquid soaked cotton fabric with an aqueous extract within a pH range of 1-14, and an impregnation liquid is a disodium hydrogen phosphate-citrate buffer solution or borax-boric acid buffer with different pH values. The proficiency testing sample can be stored and transported at room temperature, and the uniformity and the stability can fulfill the requirements on CNAS-GL03 proficiency testing sample uniformity and stability assessment guidelines. The invention also provides a preparation method of the proficiency testing sample. The method is simple and has high success rate.