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Method for producing ethanol from lignocellulose biomaterial by use of neu-heat-resistant enzyme

A technology of lignocellulose and biomass, applied in biochemical equipment and methods, biomass pretreatment, solid-phase fermentation bioreactor, etc., can solve problems such as fiber breakage and increased accessibility

Inactive Publication Date: 2005-03-16
RES CENT OF ENERGY SOURCE ENVIRONMENT & TECH
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  • Abstract
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AI Technical Summary

Problems solved by technology

The result is altered microfilament packing within the cell wall and fragmentation of fibers, resulting in increased accessibility of cellulose to hydrolytic enzymes

Method used

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  • Method for producing ethanol from lignocellulose biomaterial by use of neu-heat-resistant enzyme

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Embodiment Construction

[0039] The feedstocks used in the present invention are mainly cellulose containing materials such as forestry and agricultural residues, pulp, lignocellulosic crop biomass and organic fractions of household waste. Normally, this material has been air dried and contains 10-15% moisture.

[0040] Although the material needs to be comminuted prior to pretreatment, the required particle size (15-30mm) is considerably larger than that used in other reactor designs, thereby reducing the energy consumption associated with comminuting. This steam heat treatment causes condensation and produces a wet lignocellulosic mass. Since the temperature is high enough to thermodynamically force the dissociation of liquid water, autohydrolysis occurs, producing an acidic medium and overcoming the energy barrier for hydrolysis. Since the diffusion of the vapor phase is greater than that of the liquid phase, the incorporation of vapor into the structure of the lignocellulosic material is ensured....

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Abstract

It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230 DEG C for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of beta -glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42 DEG C.

Description

technical field [0001] The present invention relates to an improved process for obtaining ethanol from lignocellulosic biomass through simultaneous saccharification and fermentation processes. More specifically, the present invention relates to hydrothermal steam explosion pretreatment of lignocellulosic biomass at an early stage, followed by simultaneous hydrolysis (using commercially available cellulases) and novel Kluyveromyces marxianus) heat-resistant strains for fermentation. Background technique [0002] Obtaining ethanol fuel from biomass brings security to the supply of energy as it can replace fuels of fossil origin. Obtaining ethanol fuel from biomass is also conducive to regional development, with employment-related benefits. Like other renewable energy sources, the production and use of bioethanol in the transportation industry is superior to oil-derived fuels because of reduced emissions of pollutants and no increase in the greenhouse effect. [0003] Ethano...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10
CPCC12M45/20C12M21/16Y02E50/16C12P7/10C12M45/02C12M45/04Y02E50/10
Inventor I·B·伯戴希斯M·M·B·伯戴希斯J·M·O·多明戈兹J·C·加西亚
Owner RES CENT OF ENERGY SOURCE ENVIRONMENT & TECH
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