811results about "Solid phase fermentation bioreactors" patented technology

It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230° C. for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of β-glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42° C.

According to one embodiment of the invention, a system for processing biomass comprises a chamber, a biomass input device, a fluid input device, and a retrieval device. The chamber is defined by at least a bottom, at least one wall, and a cover supported by the at least one wall. The biomass input device operable to deliver biomass into the chamber to form a biomass pile. The fluid input device is operable to deliver fluid into the chamber to the biomass pile. The retrieval device operable to receive fluid from the chamber.

This invention relates to a method and system of preparing ethanol based on the solid state fermentation of sweet sorghum stem, belonging to the ethanol production technology of solid state fermentation. It includes the following key processes and device: raw materials smash is to choose the suitable mill to smash sweet sorghum stalk, strain addition is to add ethanol bacteria at the entrance of solid-state fermentation tank, solid-state fermentation is that the raw materials added bacteria is fermented in the slant rotor solid-state fermentation tank, solid distillation is to distill the solid-state fermented materials to obtain ethanol.

Disclosed is an efficient method for culturing an edible basidiomycetous fungus such as Mushroom Agaricus Blazei Murill in a liquid culture medium to give fungus aggregates of several centimeter size. Characteristically, the liquid culture medium is formulated with sucrose as a carbon source in the form of crude cane sugar in combination with a water-insoluble growth-supporting material in the form of a fine powder to serve as the core of the fungus aggregates as selected from crushed sugarcane, sugarcane bagasse, pine trees and wheat bran. Further characteristically, the culturing procedure is carried out under an oxygen-enriched condition by blowing oxygen-enriched air of at least 30% by volume oxygen into the culture medium under pressurization at 0.12 to 0.5 MPa (absolute) in a specified blowing rate.

The invention provides a microbiological solid-state seed producing and fermenting device, comprising a fermenting system, an air supply system, a temperature control system, a solid material system, a liquid-state material system and an electric control system, wherein the fermenting system comprises a fermenting tank provided with a stirring device, the top part of the fermenting tank is provided with a material liquid inlet, an exhaust port, a pressure gauge interface and an air inlet sequentially, a feed port is arranged on the upper right side of the front end of the fermenting tank, a discharge port is arranged on the lower left side of the front end of the fermenting tank, and the middle parts of both the feed port and the discharge port are provided with observation windows; the solid material system comprises a feeding device and a conveyer belt; the electric control system mainly comprises a stirring control unit, a temperature control unit, a material liquid control unit, an air supply control unit, a feeding and discharging control unit and a display. The device provided by the invention integrates the functions of steam sterilizing, fermenting, inoculating, drying and crushing of a solid culture medium, the automation degree is high, the microbial contamination rate is low, the production time is greatly shortened, the production efficiency is improved, and the stability of quality of a product is guaranteed.

The invention belongs to the field of reclamation of solid wastes, in particular relating to a method and a device for producing methane by using a dry anaerobic digestion for urban sludge and an organic matter. The device comprises a reaction chamber and a double-layer helix ribbon type agitator and is provided with a feed inlet and outlet, a sample port, a temperature-setting controller, a methane metering device, a stirring control device and others devices. The method comprises the following steps: dewatering sludge is placed into a fermentation tank for inoculation and seal, the revolving speed at start-up stage is 5-15r / min and daily stirring time is 12h, the revolving speed at acid-producing stage is 10-20r / min, intermittent operation is carried out; the daily stirring time is more than or equal to 8h and less than or equal to 16, and starting and stopping frequency for the motor is required to be increased; temperature control is performed at acid-producing stage and methane-producing stage and material temperature is controlled to be 35+ / -1 DEG C; and after finishing the fermentation, one fourth to one third of biogas residues are left for being used as inoculum for the next batch of fermented materials and the rest of residues are discharged. The invention can be applied to reclamation treatment of the urban sludge and can obviously save equipment investment and land occupation compared with the wet fermentation.

The invention discloses a solid state fermentation cylinder and a solid state fermentation method. The solid state fermentation cylinder comprises a cylinder body and a jacket provided with an upper port and a lower port, the upper end of the cylinder body is an upper end cover, a mixing system capable of lifting is arranged on the upper end cover, and according to the mixing system, a mixing frame is driven by a driving system. The mixing frame is formed by a wall surface scraper plate, a bottom surface scraper plate and mixing blades, the wall surface scraper plate and the bottom surface scraper plate form a frame-shaped structure, and the mixing blades are fixedly connected in the frame-shaped structure. The solid state fermentation cylinder and the solid state fermentation method effectively resolve the problem of low fermentation performance caused by uneven thickness and uneven heating of a feed layer. According to the solid state fermentation cylinder and the solid state fermentation method, all the processes from feeding to discharging are concentrated in the solid state fermentation cylinder, the process of transportation of materials is simplified, and the mixing system and a cooling thermal-insulation system of equipment are strengthened. Automatic bulk production of solid state fermentation can be achieved, the pure culture in the solid state fermentation process is effectively guaranteed, quality and safety of products are improved, and homogeneity property in the same bulk is guaranteed.

The invention relates to a method for separating ethanol by using Chinese sorghum straw through solid fermentation method and the system, belonging to field of ethanol produ ction process through solid fermentation. It comprises following steps: disintegrating Chinese sorghum straw, solid fermenting and getting fermentation material, conveying it to distilling system through compact channel convey belt; feeding fermentation material into annular rotary disk, contacting with steam from high- efficiency gas distributor to make ethanol converse into gas phase from solid phase, discharging slag continuously; separating ethanol with distilling- rectifying coupled process, getting industrial ethanol of 95.5% (w / w). The ethanol productivity is increased by employing istilling- rectifying coupled process, the ethanol volatilisation is dramatically reduced during conveying process by employing compact channel blet convey; the transiation is improved and continuous production is realized by employing annular rotary disk for solid distillation.

The invention relates to an organic waste dry anaerobic high-temperature fermentation system and a fermentation process. The fermentation system comprises a feeding system (1), a dry anaerobic fermentation tank (2) with an agitating system (3), a heat preservation and heating system (4), a biogas slurry and biogas residue treatment system (5), an exhaust system (6) and a control system. The organic waste dry anaerobic high-temperature fermentation system and the fermentation process have the beneficial effects that harmless treatment, reduction treatment and resource treatment can be carried out on domestic organic wastes, so that the cyclic utilization of the wastes is realized; and moreover, the treatment efficiency is high, and the energy consumption and the treatment cost are low.

The present invention relates to a gas-phase double-dynamic solid fermentation techinque, and is characterized by that the solid material to be fermented is placed into a double-dynamic environment with pressure pulsatino and circular flowing air to make solid fermentation. Its fermentation equipment includes a horizontal cylindrical tank with a quickly-opening door, in the interior of the tank arectangular spacing cylinder which is formed from four partition boards and whose section is quadratic is axially placed, and in the space of partition board and tank wall a cooling calandria parallel to the partition board is arranged, and the vertical centre of the spacing cylinder is equipped with horizontally-arranged several groups of cooling calandria.

The invention discloses anaerobic fermentation equipment for organic garbage. The equipment comprises a shell, a cutting device, an extrusion device, a solid waste treatment device and a waste liquidtreatment device, the cutting device is installed in the shell, and the extrusion device is installed at the lower end of the shell; the solid waste treatment device is installed at the lower end of the extrusion device, the waste liquid treatment device is installed at the lower end of the solid waste treatment device, the cutting device comprises a motor, and a turntable is fixedly installed atthe front end of the motor. By arranging the cutting device, the phenomenon that the garbage adheres to blades, so that the smashing and cutting effects are influenced is avoided, it is guaranteed that the surfaces of the blades are kept clean, the organic garbage is cut more completely, and smashing is more complete; by arranging the extrusion device, the solid waste treatment device and the waste liquid treatment device, the organic garbage is treated more completely, the environment is protected, and the physical health of people is also ensured.

It includes the stages of grinding the lignocellulosic biomass to a size of 15-30 mm, subjecting the product obtained to steam explosion pre-treatment at a temperature of 190-230 DEG C for between 1 and 10 minutes in a reactor (2), collecting the pre-treated material in a cyclone (3) and separating the liquid and solid fractions by filtration in a filter press (9), introducing the solid fraction in a fermentation deposit (10), adding a cellulase at a concentration of 15 UFP per gram of cellulose and 12.6 International Units of beta -glucosidase enzyme dissolved in citrate buffer pH 4.8, inoculating the fermentation deposit (10) with a culture of the heat-tolerant bacteria Kluyveromyces marxianus CECT 10875, obtained by chemical mutagenesis from strain DER-26 of Kluyveromyces marxianus and shaking the mixture for 72 hours at 42 DEG C.

The invention discloses a full-automatic control multifunctional solid-state fermentation tank, comprising a round vertical fermentation tank main body and a fermentation control system, wherein a loading auger is arranged at one side of the upper end of the fermentation tank main body; multiple clapboards are arranged in the fermentation tank main body; a high-material-bed material level automatic control device is arranged in each fermentation layer; a sterile air distribution system is arranged in each fermentation layer and is connected with a sterile air generation tank positioned at the outer side of the fermentation tank main body; a main shaft is rotationally arranged in the fermentation tank main body; and the upper ends of the clapboards are provided with stirring blades. The full-automatic control multifunctional solid-state fermentation tank disclosed by the invention integrates eight functions including dry material mixing, watering material mixing, steam sterilization, inoculation, automatic fermentation control, low-temperature drying, raw material crushing, automatic discharging and finished product smashing, and enables all operations of solid-state fermentation to be completed in a multifunctional biological solid-state fermentation tank; and by virtue of the equipment disclosed by the invention, the fermentation and production preparation processes of solid biological products can be continuously completed.

The invention relates to a solid fermentation bioreactor. The reactor comprises a reaction casing , and a porous material layer separator plate (5) is arranged in the reaction casing to divide the reaction casing into an upper fermentation chamber (1) and a lower layer air chamber (3); the reactor also comprises a fermentation control system for controlling a stirring unit, a temperature control unit, a humidity control unit, an air feed control unit and a fermentation tail gas detection and processing unit, so as to realize real time monitoring and control on temperature, humidity, ventilation, stirring and tail gas. The invention realizes totally enclosing on-line fermentation and prevents extraneous pollutants from entering; the tail gas detection system carries out real time on-line monitoring on fermentation gas, and tail gas is treated by tail gas recovery equipment to protect the environment, thereby realizing coupling operation of temperature, humidity, ventilation, stirring and tail gas control system and ensuring necessary essential factors for microbe growth.

The invention relates to an integrated methane dry fermentation device and belongs to the field of energy conservation. The device consists of two parts, namely an anaerobic fermentation tank 9 of an inner cylinder and a stack retting tank 5 of an outer cylinder; and the anaerobic fermentation tank 9 of the inner cylinder is wrapped by the stack retting tank 5 of the outer cylinder, wherein the stack retting tank 5 of the outer cylinder consists of an outer cylinder feed port 10, an outer cylinder discharge port 16, a heat insulating layer 15, a thermal reflecting film 26, an outer cylinder filter screen 18, an outer cylinder fermenting liquid outlet 25, a liquid storage box 22, an air draft cover 27, an air cap 3, an air guide pipeline 4, a gravel stratum 17 and a net 11; and the anaerobic fermentation tank 9 of the inner cylinder consists of an inner cylinder feed port 6, an inner cylinder discharge port 19, an air outlet 8, a temperature sensor 12, an inner cylinder filter screen 20, an inner cylinder fermenting liquid outlet 21, a temperature controller 13, a liquid storage box 22, an electric heater 24, a circulating water pump 14, a liquid storage pipeline 1, a one way valve 2 and a nozzle 7. The device strengthens a ventilation control system, a liquid stream stirring system and a temperature controlling system of the methane dry fermentation device, has the advantages of reasonable structure, high processing efficiency, energy conservation, convenient subsequent processing, no environmental pollution and the like and is in particular suitable for the methane fermentation of a low-temperature region.

The invention discloses an integral fuel-ethanol production process based on saccharide raw materials, and a device thereof, which belongs to the technical field of biomass liquid fuel production. The technical process comprises the following steps: grinding saccharide crops and allowing the ground saccharide crops to enter a device integrating solid-state fermentation, ethanol gas extraction and pneumatic dregs drying; mixing the ground material with high-yield ethanol yeast liquid and completing fermentation in anaerobic environment under the condition of transmitting strengthening heat mass; separating the ethanol through gas extraction; and drying dregs rotated in a tube body of a fermentor through gas flow and discharging the dregs out of the fermentor. The invention has the advantages of simplifying the process flow of producing the ethanol through solid fermentation, realizing high-degree technical integration, transforming fermentable sugar in the ground material to the utmost extent, efficiently extracting the ethanol in solid fermentation materials, utilizing flue gas and superheated steam (CO2 or heated dry air) to separate the ethanol, utilizing the flue gas (or the heated dry air) to dry the dregs, saving material-conveying time, reducing energy consumption and embodying that a solid fermentation method for producing fuel ethanol has the advantages of high net energy ratio and low environmental load.

The invention discloses a spiral self-stirring dry fermentation device and a method. Fermentation cylinders are obliquely arranged at the bottom in a gas storage tank which is installed on a top board of a leachate holding tank; an enclosed inner cylinder is arranged at the central part in each fermentation cylinder along an axial direction; a leachate jet pipe is mounted in the inner cylinder; nozzles are distributed on the leachate jet pipe and extend out from the inner cylinder; spiral separator plates are distributed in each fermentation cylinder along an axial direction, and initiating terminals of the spiral separator plates are located behind a feed inlet; a lower end of each fermentation cylinder is provided with a material outlet, a slag removal opening communicating with the material outlet of the fermentation cylinder is mounted on a base plate of the gas storage tank, and a slag removal door is arranged on the slag removal opening; the leachate holding tank is provided with a leachate return pipe communicating with the gas storage tank and a pipeline communicating with a leachate inlet of the leachate jet pipe; electric heating pipelines are distributed in the leachate holding tank; and a biogas conveying pipeline communicating with the upper part of the gas storage tank is arranged at the upper part of the leachate holding tank. The invention also provides the method of using the device for fermentation.

The invention belongs to the field of microalgae culture, and in particular relates to a half-dry solid-state adherent culture device for microalgae industrial production. The half-dry solid-state adherent culture device comprises a bracket system, adherent culture units, a supporting shaft transmission system, a speed regulating motor, liquid supplementation pipes, collection grooves and a culture medium liquid storage tank, wherein at least one adherent culture unit is arranged on the bracket system and is connected with the speed regulating motor through the supporting shaft transmission system; the liquid supplementation pipes are respectively arranged above the adherent culture unit and are communicated with the culture medium liquid storage tank through pipelines; the collection grooves are respectively formed below the adherent culture unit and are communicated with the culture medium liquid storage tank through liquid collection pipes; a liquid circulating pump and a carbon supplementation device are arranged in the culture medium liquid storage tank. The half-dry solid-state adherent culture device can be used for producing microalgae biomass and secondary metabolites on a large scale; the yield in unit floor area is effectively improved; the culture period is shortened; the culture water consumption is greatly reduced; the cost of the culture device and the operation cost are reduced; the industrial magnification is facilitated.

A bioreactor includes a nutrient reservoir, a rotatable culture bed, at least one culture carrier medium, a mist generator and a mist delivery pipe. The nutrient reservoir stores therein a nutrient solution required for the fungus, plant cell, tissue, organ, hairy roots and plantlet. The rotatable culture bed is rotated at a specified speed. The at least one culture medium has thereon the fungus, plant cell, tissue, organ, hairy roots and plantlet and is supported on the rotatable culture bed. The mist generator is used for producing mists of the nutrient solution. The mist delivery pipe delivers therein the mists of the nutrient solution and has a mist-exit port adjusted to a desired level above the culture medium so as to discharge and spray the mists over the fungus, plant cell, tissue, organ, hairy roots and plantlet.

A system for removing particulate, sulfur dioxide, nitrogenous oxides and other condensables from the flue gas of a coal, lignite, or other solid fuel combustor. The system can combine biomass filtration, anaerobic digestion, steam absorption refrigeration, and heat exchangers to minimize parasitic energy consumption, solid and liquid waste disposal and interference with combustion efficiency, while achieving near total reduction of contaminants without regard to sulfur or ash content of fuel. Gases at the exit of a multiclone of a fuel-combustor enter a waste heat boiler, generating low pressure steam that drives a steam absorption chiller. The flue gas which still contains small particulate material pass through a moving belt system conveying a mat of chopped biomass that can be impregnated with bacteria that feed on nitrogenous oxides. The mat is sufficiently thick and moist to entrap the remaining solids and smoke in the exhaust stream. The particulate-free flue gas passes through a staged heat exchanger which can utilize ambient air, pond water, and the refrigerated water from the steam absorption chiller to condense the water vapor in the exhaust stream. Sulfur oxides exits the flue gas stream with the condensed water vapor which can be processed in an anaerobic digester. Methanogenic bacteria within the digester can convert the mat and the acidic condensate into a biogas, a combination of methane and carbon dioxide, and plant growth media.

A bioreactor includes a nutrient reservoir, a rotatable culture bed, at least one culture medium, a mist generator and a mist delivery pipe. The nutrient reservoir stores therein a nutrient solution required for the fungus, plant cell, tissue, organ, hairy roots and plantlet. The rotatable culture bed is rotated at a specified speed. The at least one culture medium has thereon the fungus, plant cell, tissue, organ, hairy roots and plantlet and is supported on the rotatable culture bed. The mist generator is used for producing mists of the nutrient solution. The mist delivery pipe delivers therein the mists of the nutrient solution and has a mist-exit port adjusted to a desired level above the culture medium so as to discharge and spray the mists over the fungus, plant cell, tissue, organ, hairy roots and plantlet.

A bioreactor culture system and process for producing conifer somatic embryos comprise a closed vessel, a biomass immobilization matrix, a liquid culture medium recirculating equipment, and a gas control equipment. The biomass immobilization matrix is installed in a closed vessel, a liquid culture medium is introduced in the closed vessel, and the level of liquid culture medium in the closed vessel is kept lower than the biomass immobilization matrix. The liquid culture medium recirculating equipment sprays liquid culture medium from the closed vessel onto the biomass immobilization matrix to thereby irrigate the maturing immobilized biomass.

An automated method for the production of cells and / or biomolecules such as protein or peptides includes culturing cells in at least one high cell density bioreactor, thereby fluidly connecting said bioreactor with a culture medium supply and a gas or gaseous mixture; fluidly connecting said bioreactor with a downstream unit; and growing cells to a density at least 50 million cells per ml. The total volume of the bioreactor is at least 10 liters. A system suitable for implementation of the automated method above is a small-scale and cupboard-sized system, which can be placed in a portable clean room.

The invention discloses a tower solid fermentation device, which comprises a fermentation storage bin. The fermentation storage bin is formed by superimposing a plurality of layers of fermentation bins; the uppermost layer of fermentation bin is provided with a feed inlet; the bottommost layer of fermentation bin is provided with a discharge hole; and a clapboard is arranged between two adjacent layers of fermentation bins, and fermentation materials can be discharged into the lower layer of fermentation bin through the clapboard. The tower solid fermentation device has the advantages of reasonable structure, small occupied area, high work efficiency, low energy consumption and labor saving. Each layer of fermentation bin can be linked and jogged and fermentation adjustment of each layer can be performed separately or simultaneously. The materials are fermented from top to bottom layer by layer, and the fermented materials on the bottommost layer are continuously moved away and the materials are continuously fed from the uppermost layer at the same time.

The invention discloses a dry type anaerobic fermentation tank, belonging to anaerobic fermentation equipment. Biogas slag and biogas slurry are added in a batching water-seal tank, a feed conveying assembly communicates the batching water-seal tank and a fermentation tank body, the fermentation tank body is internally provided with a feed-frame empty-mixing preventer driven by a first motor, a fermentation tank base is internally provided with a spiral discharging machine driven by a second motor, a screen, a wedge-shaped feed collecting groove and a heating pipe, an extrusion and discharge shell with a discharge hole is arranged on the side wall face of the fermentation tank base and is internally provided with a piston, a pressure spring and a spring seat, an end cover provided with a pressure regulating handle is arranged on the outer end face of the extrusion and discharge shell, the pressure regulating handle and the spring seat are connected together or in contact fit, one end part of the spiral discharging machine is located in the extrusion and discharge shell, a spraying connection pipe communicates a circulating pump and the fermentation tank body, and the circulating pump is communicated with a percolate tank. The tank has novel and reasonable structure, high production efficiency and wide applicable range, and is reliable to run and simple and convenient to use and operate.

The invention discloses a breeding pollution comprehensive treatment system and method of a large-scale pig farm. The system comprises a source separation pigsty, a pigsty washing water treatment system, a solid-liquid separation system, a solid high-temperature aerobic fermentation system, a liquid high-temperature aerobic fermentation system, an odor and flue gas treatment system, a boiler system and a detection control system. The source separation pigsty is designed to be a pigsty with fecaluria separated from rainwater, pigsty washing water and drinking residual water. A sludge pump pumps the fecaluria to a solid-liquid separation device. Solids separated out from the solid-liquid separation device are conveyed to the solid high-temperature aerobic fermentation system. Liquid separated out from the solid-liquid separation device is conveyed to the liquid high-temperature aerobic fermentation system. A fermentation odor exhaust opening of the solid high-temperature aerobic fermentation system, a fermentation odor exhaust opening of the liquid high-temperature aerobic fermentation system and exhausted flue gas of the boiler system are connected with the odor and flue gas treatment system through pipelines. The boiler system comprises a boiler, a circulation pump, a hot water pipeline and a water return pipeline. Sensors of the detection control system are arranged in the above systems, and the detection control system carries out connection control over the above systems. According to the system and method, pollution of the large-scale pig farm is comprehensively treated, and zero emission, zero pollution and resourceful utilization of pollutants of the large-scale pig farm are achieved.

The invention belongs to the field of microalgae culture, and particularly relates to an inserting-plate type microalgae semi-dry solid adherent culture device. The inserting-plate type microalgae semi-dry solid adherent culture device is characterized by comprising a supporting truss, culture plates, liquid distributing pipes, liquid collecting tanks and a liquid storage bottle, wherein the supporting truss is provided with at least one culture plate, and one liquid distributing pipe is arranged above each culture plate; the liquid collecting tank arranged on the supporting truss is arranged under each culture plate; all the liquid collecting tanks are communicated with the liquid storage bottle by the main liquid pipe; the bottom of the liquid storage bottle is respectively provided with a circulating pump and a carbon-supplementing gas distributing pipe communicated with a carbon dioxide source, and the circulating pump is communicated with a liquid distributing pipe by a pipeline; and each culture plate is provided with a picking scraper. The inserting-plate type microalgae semi-dry solid adherent culture device has the advantages that the yield in unit occupied area is effectively increased, the culture period is shortened, the water consumption for culture is greatly reduced, the cost and the operating cost of the culture device are reduced and the industrial culture on large scale is easily realized.

The invention discloses an automatic chain-type solid-state fermentation reaction device, which comprises a inoculation barrel, a spiral conveyer and a fermentation device which are connected in sequence, wherein the fermentation device comprises at least two fermentation beds which are arranged sequentially and can automatically convey and output material. The position of the input end of the fermentation bed behind can just receive the falling material from the output end of the fermentation bed before. Each section from the preparation of liquid seeds to the solid fermentation, as well as to the processing procedure after the fermentation, the mechanical automation operation is realized, labor intensity is considerably reduced, the mistakes caused by manual operation in the course of fermentation are eliminated, and the disadvantage that the quality of the fermentation product is not easy to keep table is overcome.