400 results about "Tetramethyl benzidine" patented technology

The invention belongs to the technical field of in-vitro immunodetection and in particular relates to a kit for detecting myeloperoxidase (MPO) concentration in a sample and a preparation method of the kit. The kit comprises a porous plate coated with an anti-MPO monoclonal antibody, an MPO series standard substance and a quality control material, a horse radish peroxidase-labeled anti-MPO monoclonal antibody, a sample buffer solution, a citrate buffer solution of hydrogen peroxide, a citrate buffer solution of tetramethyl benzidine, a 1-2M sulfuric acid solution, and a phosphate buffer solution containing surfactants. Because the used detection instruments are simple and are universal instruments, the kit is low in detection cost and contributes to popularization; the kit is easy to operate and can be realized without professionals. Meanwhile, according to the unique standard diluent and sample buffer solution formula in the kit, the reliability and the accuracy of the detection result are greatly improved.

The invention discloses a method for measuring nano-gold mimetic peroxidase based urease and an inhibitor thereof. The method is characterized by comprising the following steps: coupling a urease catalysis urea reaction system with a nano-gold mimetic peroxidase-hydrogen peroxide-3,3',5,5'-tetramethylbenzidine dihydrochloride catalytic chromogenic reaction system, and combining with the changes of solution colors and ultraviolet absorption spectrum characteristics to directly measure the content of urease and the inhibitor thereof, wherein in a range of 1.8-90U / L, A450 and the urease concentration have a linear relationship, and the limit of detection is 1.8U / L; and by virtue of software fitting, IC50 of obtained acetohydroxamic acid of the urease inhibitor is 0.05mmoL / L. The method disclosed by the invention is simple and convenient to operate and high in sensitivity, and can be applied to the measurement of the content of urease and the inhibitor thereof in environment and life science systems as an analysis method.

The invention discloses a novel method for detecting lead ions based on the sensitivity of a DNA-MOF material. The method comprises the following steps: preparing a gold-paper chip working electrode Au-PWE; synthesizing Au-MOF from 5,10,15,20-tetra(4-methoxy phenyl)porphyrin, ferric chloride and nanogold; carrying out functionalization by virtue of GR DNA, so as to synthesize a GR-Au-MOF material; modifying the prepared Au-PWE with HP DNA, and dropwise adding a GR-Au-MOF solution mixed with 3,3',5,5'-tetramethyl benzidine, hydrogen peroxide and a sample solution to HP modified Au-PWE; and connecting the modified chip to an electrochemical working station by virtue of a three-electrode system, and detecting the concentration of lead ions by virtue of a differential pulse voltammetry. The novel method for detecting the lead ions is high in sensitivity and good in selectivity, and the real-time monitoring of samples can be realized.

The invention relates to a human anti-rabies virus IgG antibody ELISA test kit. An ELISA plate is firstly coated with an anti-rabies virus monoclonal antibody, wherein the coating buffer solution is a 0.05M carbonate buffer solution of which the pH value is 9.6, and the coating amount is 0.1-1ug per hole; a blocking solution is a BSA or skimmed milk of which the mass concentration is 1-10%; the ELISA plate is coated with a rabies virus purified antigen after being blocked, wherein the coating amount is 0.1-1ug per hole; a sample diluent is a 0.01mol / L phosphate buffer solution (PBS) which contains bovine serum albumin (BSA) with a mass concentration of 0.1-10% and NaN3 with a mass concentration of 0.01-0.05 and has a pH value of 7.2-7.4; an enzyme conjugate is a horse radish peroxidase-mouse anti-human IgG enzyme conjugate; a concentrated cleaning solution is a 0.01mol / L PBS which contains tween-20 with a volume concentration of 0.05% and has a pH value of 7.2-7.4; a zymolyte A solution is a 3,3'-5,5'-tetramethyl benzidine solution, and a zymolyte B solution is an oxydol solution; and a stop solution is a 1mol / L H2SO4 solution, and a positive control and a negative control are arranged in the kit. The specificity of the kit is up to 100%, and the sensitivity is 1:640. The kit is used for evaluating the immunity effect of humans inoculated with rabies vaccines.

An enzyme-linked detection kit for detecting VEGF. A kit body contains an enzyme-linked reaction plate coated by a VEGF acceptor and a monoclonal antibody, an enzyme conjugate labelled by HRP and configured by antibody and a protective agent, a freeze-drying powder of the VEGF protein as a positive contrast, a protein freeze-drying powder as a negative contrast, a sample diluent, a PBST concentrated lotion, a chromogenic substrate configured by 3,3',5,5'-tetramethyl benzidine, 2MH2SO4 as a stopping solution and a seal plate gummed paper. The kit can be used in diagnosis and prognosis of cancer, angiopathy, diabetes, retinopathy and rheumatoid arthritis, and has advantages of accuracy, specificity, sensitivity, stability and convenience, etc.

A method for amperometric detection of proteins, especially haemoglobin in faeces, using an electrochemical sensor. The electrochemical sensor includes: a working electrode having an electrically conductive matrix holding a first reagent and / or a second reagent, the second reagent being an oxidising agent, or a precursor thereof, for the first reagent; a counter electrode and optionally a reference electrode; wherein a reaction between the first reagent and the oxidising agent is catalysed by the protein to provide a detectable signal at the working electrode. The electrically conductive matrix is an electrically conductive carbon- or graphite-containing matrix or an electrically conductive porous matrix.