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Visualization method for rapidly detecting trace amount of uranyl ions in water environment

A technology for detecting water and trace uranium, which is applied in color/spectral characteristic measurement, material analysis by observing the influence on chemical indicators, and analysis by making materials react chemically, etc. Detection, low anti-matrix interference ability, low sensitivity and other problems, to achieve good anti-matrix interference ability, strong anti-matrix interference ability, and high sensitivity

Inactive Publication Date: 2015-10-07
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing sensing detection methods cannot be used for trace uranyl ions (UO) in actual environmental water samples due to low sensitivity, poor selectivity, or low ability to resist matrix interference. 2 2+ ) on-site rapid detection

Method used

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  • Visualization method for rapidly detecting trace amount of uranyl ions in water environment
  • Visualization method for rapidly detecting trace amount of uranyl ions in water environment
  • Visualization method for rapidly detecting trace amount of uranyl ions in water environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Preparation of DNAzyme functionalized magnetic beads: Take 2.0 μL of substrate strand DNA (sequence: 5'-biotin-ATATAT TGT CCG TGC TAG AAG GAA CTC ACT AT rA GGA AGA GAT GGA CGT G- 3') and 2.0 μL of 0.36 μM enzyme chain DNA (sequence: 5'-CAC GTC CAT CTC TGC AGT CGG GTA GTT AAA CCG ACC TTC AGA CAT AGT GAG T-3') mixed in 46 μL of MES hybridization Buffer (50 mM MES, 300 mM NaNO 3 , pH=5.5), heated to 85 ℃ for 2 min, then cooled to room temperature at a rate of 1 ℃ / min to obtain a double-stranded DNAzyme solution; then, 10 μL of magnetic bead dispersion with a concentration of 1 mg / mL (surface Modified Streptavidin; diameter: about 1 μm), the mixed solution was shaken at 37 °C for 30 min to evenly immobilize the DNAzyme on the surface of the magnetic beads. Use a magnet to separate the magnetic beads immobilized with DNAzyme double strands, and wash with 100 μL MES hybridization buffer for three times, then disperse the magnetic beads immobilized with DNAzyme double str...

Embodiment 2

[0033] Take 10 μL of Minjiang water sample filtered through a 0.22 μm filter membrane, operate according to the method of Example 1, and finally observe the color change, and compare the standard UO obtained in Example 1 2 2+ The color or absorbance of the ionic solution, for the UO in the water sample 2 2+ The ions are semi-quantitatively or quantitatively detected, and the detection results are as shown in Table 1, which are basically the same as the measurement results of ICP-MS, indicating that the method of the present invention is a method for visually and rapidly detecting trace uranyl ions in the water environment, and is suitable for On-site testing.

[0034] Table 1 UO in water samples from the Minjiang River using the established method 2 2+ Ion analysis results and comparison with ICP-MS detection results

[0035]

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Abstract

The invention belongs to the field of detecting a trace amount of ions in the water environment, and particularly relates to a visualization method for rapidly detecting a trace amount of uranyl ions in the water environment. The method mainly includes the steps that DNAzyme with the specific recognition function on UO2 <2+> is fixed to the surfaces of magnetic beads, and horse radish peroxidase is preassembled on the surface of nano-gold; then the magnetic beads are connected with the nano-gold through the cutting effect of the UO2<2+> on the DNAzyme and the hybridization reaction of DNA, after separation and collection are carried out through an external magnetic field, H2O2 oxidation tetramethyl benzidine is efficiently catalyzed through the horse radish peroxidase to enable a solution to be changed from the blank to the blue, and therefore sensitive and specific visualization rapid detection of the UO2<2+> ions is achieved. As the method has the advantages of being high in sensitivity, high in specificity, high in matrix interference resistance, simple, rapid, low in cost and the like, the method can be used for site rapid visualization detection of the trace amount of UO2<2+> ions in various water samples.

Description

technical field [0001] The invention belongs to the field of detection of trace ions in water environment, and in particular relates to a method for visually and rapidly detecting trace uranyl ions in water environment. Background technique [0002] Uranium is a natural radioactive element that contains three radioactive isotopes, uranium-234, uranium-235, and uranium-238. The natural abundances of each isotope are 234 U (0.0050-0.0059%), 235 U (0.7198-0.7202%), 238 U (99.2739-99.2752%). About 48% of uranium's radioactivity comes from 234 U, 2.2% from 235 U, 48.9% from 238 U. The radioactive isotopes of uranium have a particularly long half-life of 234 U (244,000 years), 235 U (710 million years), 238 U (4.5 billion years). With the development of economy and science and technology, the process of industrialization has been accelerated. As an important strategic energy source, uranium has been widely used in military, industrial and commercial fields because of its...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/78
Inventor 付凤富张红艳阮雅娟
Owner FUZHOU UNIV
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