Visualization method for rapidly detecting trace amount of uranyl ions in water environment
A technology for detecting water and trace uranium, which is applied in color/spectral characteristic measurement, material analysis by observing the influence on chemical indicators, and analysis by making materials react chemically, etc. Detection, low anti-matrix interference ability, low sensitivity and other problems, to achieve good anti-matrix interference ability, strong anti-matrix interference ability, and high sensitivity
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Embodiment 1
[0028] (1) Preparation of DNAzyme functionalized magnetic beads: Take 2.0 μL of substrate strand DNA (sequence: 5'-biotin-ATATAT TGT CCG TGC TAG AAG GAA CTC ACT AT rA GGA AGA GAT GGA CGT G- 3') and 2.0 μL of 0.36 μM enzyme chain DNA (sequence: 5'-CAC GTC CAT CTC TGC AGT CGG GTA GTT AAA CCG ACC TTC AGA CAT AGT GAG T-3') mixed in 46 μL of MES hybridization Buffer (50 mM MES, 300 mM NaNO 3 , pH=5.5), heated to 85 ℃ for 2 min, then cooled to room temperature at a rate of 1 ℃ / min to obtain a double-stranded DNAzyme solution; then, 10 μL of magnetic bead dispersion with a concentration of 1 mg / mL (surface Modified Streptavidin; diameter: about 1 μm), the mixed solution was shaken at 37 °C for 30 min to evenly immobilize the DNAzyme on the surface of the magnetic beads. Use a magnet to separate the magnetic beads immobilized with DNAzyme double strands, and wash with 100 μL MES hybridization buffer for three times, then disperse the magnetic beads immobilized with DNAzyme double str...
Embodiment 2
[0033] Take 10 μL of Minjiang water sample filtered through a 0.22 μm filter membrane, operate according to the method of Example 1, and finally observe the color change, and compare the standard UO obtained in Example 1 2 2+ The color or absorbance of the ionic solution, for the UO in the water sample 2 2+ The ions are semi-quantitatively or quantitatively detected, and the detection results are as shown in Table 1, which are basically the same as the measurement results of ICP-MS, indicating that the method of the present invention is a method for visually and rapidly detecting trace uranyl ions in the water environment, and is suitable for On-site testing.
[0034] Table 1 UO in water samples from the Minjiang River using the established method 2 2+ Ion analysis results and comparison with ICP-MS detection results
[0035]
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