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Mercury-ion detection method simulating peroxidase based on nano platinum and kit

A peroxidase and detection kit technology, applied in the fields of analytical chemistry and nanometers, can solve the problems of unfavorable rapid analysis of low-concentration mercury detection, troublesome pretreatment analysis technicians, complicated operations, etc., and achieves a simple, fast, and easy preparation process. Obtain and test the effect of high sensitivity

Inactive Publication Date: 2015-01-07
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are cumbersome to operate, require cumbersome pretreatment, specialized analytical technicians, and expensive instruments, which are not conducive to rapid on-site analysis and detection of low-concentration mercury.

Method used

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  • Mercury-ion detection method simulating peroxidase based on nano platinum and kit
  • Mercury-ion detection method simulating peroxidase based on nano platinum and kit
  • Mercury-ion detection method simulating peroxidase based on nano platinum and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Mix 1 ml of 16 mmol / L chloroplatinic acid aqueous solution with 1 ml of 40 mmol / L trisodium citrate, dilute with 38 ml of double distilled water, and stir for 30 minutes in the dark. Subsequently, 0.2 ml of 50 mmol / L sodium borohydride aqueous solution was added (the addition time was controlled within 2 minutes), the color of the reaction solution changed from light yellow to brownish yellow, and stirring was continued for 1 hour in the dark. The resulting nano-platinum concentration was 78 mg / L, and the average particle size was 2.5±0.4 nm. All glassware used in the above process was soaked in aqua regia, washed thoroughly with double distilled water, and dried.

Embodiment 2

[0039] The nano-platinum solution obtained in Example 1 was diluted 50 times with double distilled water to obtain a nano-platinum solution with a concentration of 1.56 mg / L. Take 0.1 ml of nano-platinum solution with a concentration of 1.56 mg / L, add 2.7 ml of double distilled water, 1 ml of hydrogen peroxide with a concentration of 2 mol / L, and 0.2 ml of 3,3',5 , 5'-Tetramethylbenzidine hydrochloride, after mixing, bathe in water at 45°C for 10 minutes, the color of the system will be dark blue, and the maximum absorption wavelength of the system is at 652 nm (see figure 1 ).

Embodiment 3

[0041] The nano-platinum solution obtained in Example 1 was diluted 50 times with double distilled water to obtain a nano-platinum solution with a concentration of 1.56 mg / L. In 0.1 ml of nano-platinum solution with a concentration of 1.56 mg / L, add 0.4 ml of a mercury ion sample solution with a concentration of 50 nmol / L, mix and place it at room temperature for 2 minutes, add 2.3 ml of double distilled water, 1 ml of hydrogen peroxide with a concentration of 2 mol / L and 0.2 ml of 3,3',5,5'-tetramethylbenzidine hydrochloride with a concentration of 16 mmol / L were mixed in a water bath at 45°C for 10 minutes, and the system It is light blue after color development, and the absorption value of this system at 652 nm is significantly lower than that of Example 2 (see figure 2 ).

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Abstract

The invention discloses a mercury-ion detection method simulating peroxidase based on nano platinum and a kit. After the specificity interaction of the nano platinum and mercury ions, the inhibition variation of the activity of the perioxidase is simulated, hydrogen peroxide is catalyzed by the nano platinum to oxidize 3, 3', 5, 5'-TMB HCL to achieve color developing. The invention provides a novel rapid, simple and ultrasensitive mercury ion detection method. The nano platinum used in the detection method is simple to prepare and easy to obtain, and visualized and convenient analysis on the mercury ions can be realized. The detection method has the advantages of simplicity in operation, short detection time, high sensitivity, high specificity and the like and is easy to popularize and use.

Description

Technical field [0001] The present invention involves a mercury -ion -color detection method and the detection kit based on nano -platinum simulation of peroxidase, which is an analytical chemistry and nano -technology field. Background technique [0002] As we all know, mercury is a global environmental pollutant with high toxicity, especially its characteristics of high migration, persistence, methylation, biological enrichment, and large food chain.The health of animals and plants and human beings is also a great threat.Medical research has long confirmed that a certain degree of mercury exposure will severely damage the brain, heart, kidney, lungs and immune systems of people.In the global industrialization process, the mercury content in the environment caused by human activities has increased and the scope of pollution has expanded. According to statistics, the mercury content in the atmosphere, water, and soil has increased by 3 times compared with several years ago.The US...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/33G01N21/31
Inventor 陈伟何少斌吴钢伟邓豪华彭花萍
Owner FUJIAN MEDICAL UNIV
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