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Bovine serum albumin-platinum composite nanomaterial mimetic peroxidase

A technology of bovine serum albumin and composite nanomaterials, which is applied in the field of nanotechnology and bionics, can solve the problems of limited natural enzyme sources, loss of natural enzyme activity, harsh operating conditions, etc., and achieves good stability, excellent stability, and a preparation method Easy and fast effect

Inactive Publication Date: 2013-12-11
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the sources of natural enzymes are limited, difficult to purify, and expensive
At the same time, natural enzymes are easily affected by various physical and chemical factors and lose their activity. Therefore, in practical applications, the operating conditions of experiments are relatively harsh, which greatly limits their applications.

Method used

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  • Bovine serum albumin-platinum composite nanomaterial mimetic peroxidase
  • Bovine serum albumin-platinum composite nanomaterial mimetic peroxidase
  • Bovine serum albumin-platinum composite nanomaterial mimetic peroxidase

Examples

Experimental program
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Effect test

example 1

[0035] Add 5 ml of 16 mmol / L chloroplatinic acid solution and 0.5 ml of 1.5 mol / L sodium hydroxide solution to 5 ml of 50 mg / ml bovine serum albumin aqueous solution, mix well and react in a water bath at 80 °C for 2 h. After the reaction, the solution was put into an ultrafiltration tube with a molecular weight cut-off of 3k, subjected to centrifugal ultrafiltration at 6000 r / min, and washed with water three times. The obtained solution after ultrafiltration is freeze-dried to obtain bovine serum albumin-platinum composite nanomaterial powder.

example 2

[0037] In 2780 μL of phosphate buffer (pH=4.5, 20 mmol / L), add 1 ml of hydrogen peroxide with a concentration of 2 mol / L, 0.2 ml of 3,3',5,5 with a concentration of 16 mmol / L '-Tetramethylbenzidine hydrochloride and 20 μL bovine serum albumin-platinum composite nanomaterial aqueous solution with a concentration of 0.36 mmol / L were mixed and incubated at 45 °C for 10 minutes, the solution changed from colorless to blue, and at 652 There is an absorption peak at nm ( figure 1 ).

example 3

[0039]在2780 μL磷酸盐缓冲液(pH=4.5,20 mmol / L)中依次加入1 ml浓度为2 mol / L的过氧化氢、0.2 ml浓度为16 mmol / L的3,3’,5,5’-四甲基联苯胺盐酸盐和20 μL不同浓度的牛血清白蛋白-铂复合纳米材料水溶液,混合后45 ℃温浴10分钟,测定652 nm波长处吸光度。 Depend on figure 2 可见,显色产物的吸光度随着牛血清白蛋白-铂复合纳米材料终浓度增大而增大。

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Abstract

The invention discloses bovine serum albumin-platinum composite nanomaterial mimetic peroxidase as well as a preparation method thereof and application. Bovine serum albumin is used as a template, and the bovine serum albumin-platinum composite nanomaterial mimetic peroxidase is prepared through biomineralization. Bovine serum albumin-platinum composite nanomaterials are prepared through the following method that chloroplatinic acid aqueous solutions are added to bovine serum albumin aqueous solutions and are mixed, sodium hydroxide aqueous solutions are added to obtain mixed solutions, and water bath heating is carried out; ultrafiltration is carried out on the solutions, then the solutions are washed, and bovine serum albumin-platinum composite nanomaterial aqueous solutions are obtained. The bovine serum albumin-platinum composite nanomaterials have excellent peroxidase activity, and can catalyze hydrogen peroxide oxidation 3, 3', 5, 5'-tetramethyl benzidine hydrochloride to be in color development. Meanwhile, the mimetic peroxidase resists acid and base, high temperature and high salinity, and has excellent short-term indoor temperature stability and long-term indoor temperature stability.

Description

technical field [0001] The invention relates to a bovine serum albumin-platinum composite nano material with the characteristic of simulating peroxidase, and belongs to the field of nano technology and bionic technology. Background technique [0002] Enzymes are very important proteins with catalytic activity in organisms. They have high catalytic efficiency, strong specificity, and mild reaction conditions. The growth, development, reproduction and other life activities of organisms are inseparable from the catalysis of enzymes. However, the sources of natural enzymes are limited, difficult to purify, and expensive. At the same time, natural enzymes are easily affected by various physical and chemical factors and lose their activity. Therefore, in practical applications, the operating conditions of experiments are relatively harsh, which greatly limits their applications. In recent years, the research, development and application of artificial mimetic enzymes have attracte...

Claims

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Application Information

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IPC IPC(8): B22F1/00C07K14/765
Inventor 陈伟何少斌邓豪华刘爱林林新华李光文
Owner FUJIAN MEDICAL UNIV
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