Method for detecting surviving gene based on graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor

Abstract

The invention discloses a method for detecting a surviving gene based on a graphene-gold composite material electrochemical DNA (Deoxyribose Nucleic Acid) biosensor. The method comprises the following steps: a specific probe is designed according to a gene segment to be detected; a capture probe is self-assembled on the surface of G-3DAu / GCE through a gold-sulfur bond; the capture probe and a signal probe with the tail end marked by biotin are respectively combined with a target DNA to form a 'sandwich' model in the presence of the target DNA; a horse radish peroxidase marked by avidin can be combined with the biotin marked by the signal probe, so that the HRP (Horse Radish Peroxidase) can be fixed on the surface of an electrode; the electrode is placed in a base solution of 3, 3', 5, 5'-tetramethyl benzidine (TMB) and H2O2, and the H2O2 can oxidize TMB to generate a bidiazotizedbenzidine material under the catalyzing of the HRP, so that an electrochemical signal is generated. The method has the advantages of being simple, quick and green in preparation process, and high in selectivity and sensitivity.

Description

[0001] The invention relates to a method for detecting the osteosarcoma-related gene survivin based on the graphene / three-dimensional nano-gold nanocomposite material electrochemical DNA biosensor. Background technique [0002] In recent years, electrochemical DNA sensors have been widely considered as a promising detection method in the diagnosis of gene-related diseases and other biological analyzes due to their advantages of fast response, high sensitivity, easy operation, miniaturization, and low cost. . In order to further enhance the sensitivity and selectivity of sensors, nanostructured materials such as nanoparticles, nanoporous materials, and nanocarbon materials have been widely introduced into biosensors as transducers for enhanced sensing. [0003] The carbon atom arrangement of graphene is the same as the single atomic layer of graphite. It is a single-layer two-dimensional crystal composed of carbon atoms arranged in a honeycomb lattice with sp2 mixed orbitals. ...

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Problems solved by technology

[0006] At present, clinical genetic testing methods include flow cytometry, chromosome analysis, FISH, RT-PCR, etc., but these methods have certain limitations such as time-consuming, low accuracy, and expensive.

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