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CsxWO3 type peroxide mimic enzyme, and preparation and application thereof

A technology of peroxidation and simulating enzyme, applied in the field of nanotechnology and enzyme catalysis, can solve the problems of limited source of natural enzymes, high price, difficult purification, etc., and achieve the effects of short reaction time, low cost and stable chemical activity.

Inactive Publication Date: 2014-08-13
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of glucose sensors are based on the horseradish peroxidase colorimetric method. However, the source of natural enzymes is limited, difficult to purify, and expensive. In addition, in order to maintain its cleanliness, the requirements for experimental conditions and operating environments are relatively harsh. , so that its application is greatly restricted, so the development and application research of mimetic enzymes has attracted more and more people's attention.

Method used

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  • CsxWO3 type peroxide mimic enzyme, and preparation and application thereof
  • CsxWO3 type peroxide mimic enzyme, and preparation and application thereof
  • CsxWO3 type peroxide mimic enzyme, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] At a temperature of 35 °C, 50 μg / ml of Cs x WO 3 Nanomaterials were added to 2.275ml of PBS (pH=4.0) buffer solution dissolved with TMB (0.6mM), and 50mM H 2 o 2 solution, reacted for 30min, and then measured the absorbance at 652nm on a UV spectrophotometer (see figure 1 ); figure 1 The results show that Cs x WO 3 group has obvious absorption at 652nm, indicating that Cs x WO 3 Nanomaterials have peroxidase-like activity and can catalyze H 2 o 2 Oxidation of TMB produces a color reaction.

Embodiment 2

[0035] At room temperature, 50 μg / ml of Cs x WO 3 Nanomaterials were added to PBS buffer solution (pH=4.0) containing 1 mM terephthalic acid, and 50 mM H 2 o 2 solution, start timing, at this time Cs x WO 3 Nanomaterials can catalyze H 2 o 2 Hydroxyl radicals are produced, and the produced hydroxyl radicals react with the substrate terephthalic acid to produce fluorescent substances, whose maximum absorption wavelength is 425nm, such as figure 2 shown; figure 2 The results showed that as the reaction time increased, the fluorescence value became higher and higher, indicating that a large number of hydroxyl radicals were generated, which proved that Cs x WO 3 The catalytic mechanism of nanomaterials is to catalyze H 2 o 2 produce hydroxyl radicals.

Embodiment 3

[0037] Will Cs x WO 3 The nanomaterial aqueous solution was ultrasonically treated for 0.5h, then dropped on the copper grid, dried and then scanned by TEM (see image 3 ); image 3 Show the resulting Cs x WO 3 It is in the shape of nanorods, and the size is relatively uniform. The length of the nanorods is about 150 nm and the width is 15 nm.

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Abstract

The invention provides a CsxWO3 type peroxide mimic enzyme, and a preparation method and application thereof. The CsxWO3 type peroxide mimic enzyme has the activity similar to that of peroxidase, and can be used for catalyzing H2O2 to generate a great quantity of hydroxyl free radicals and effectively oxidizing a substrate, 3,3',5,5'-tetramethyl benzidine to generate a color developing reaction. The CsxWO3 type peroxide mimic enzyme is combined with glucose oxidase for use, the content of glucose in a solution can be indirectly detected by measuring an ultraviolet absorption value of the oxidized 3,3',5,5'-tetramethyl benzidine and the detection limit is 0.1 micro milliliter. Compared with horse radish peroxidase (HRP), the CsxWO3 nano material has the advantages of simple preparation process, low cost, stable chemical activity and the like; the catalytic effect and the applicability of the CsxWO3 nano material are higher than those of natural peroxidase, and thus the CsxWO3 nano material can be used as a substitute of natural peroxidase.

Description

technical field [0001] The invention relates to the field of nanotechnology and the field of enzyme catalysis, in particular to a Cs x WO 3 Peroxide-like mimetic enzyme, its preparation method and application. Background technique [0002] Diabetes has now become a disease that affects the normal life of humans. Generally, the concentration of glucose in the human body is 3-8mM, while the concentration of glucose in the body of diabetic patients is 9-40mM. Glucose concentration is a key indicator for diagnosing diabetes. At present, there are many detection methods for detecting the concentration of glucose, such as: high performance liquid chromatography, electrochemistry, colorimetry, etc. Compared with other methods, colorimetry has attracted more attention because of its advantages of high sensitivity, easy operation and low cost. A large number of glucose sensors are based on the horseradish peroxidase colorimetric method. However, the source of natural enzymes is li...

Claims

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Application Information

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IPC IPC(8): B01J23/30C01G41/00B82Y30/00B82Y40/00G01N21/78
Inventor 朱春玲占红梅李子彬谢增鸿
Owner FUZHOU UNIV
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