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Folic acid-porous platinum-graphene oxide composite nano material as well as application thereof in detecting tumor cells

A graphene composite and nanomaterial technology, which is applied in the field of nanotechnology and medical testing, can solve problems such as difficult to achieve detection results, and achieve good peroxidase activity and rapid and sensitive detection effects

Inactive Publication Date: 2014-02-05
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional detection and localization of tumors is difficult to achieve the expected detection effect due to the lack of methods to achieve targeted positioning

Method used

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  • Folic acid-porous platinum-graphene oxide composite nano material as well as application thereof in detecting tumor cells
  • Folic acid-porous platinum-graphene oxide composite nano material as well as application thereof in detecting tumor cells
  • Folic acid-porous platinum-graphene oxide composite nano material as well as application thereof in detecting tumor cells

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0039] Weigh 1.2 g of 325 mesh flake graphite and add it to a mixed solution of 144 mL of concentrated sulfuric acid with a concentration of 18 mol / L and 16 mL of phosphoric acid with a concentration of 15 mol / L. After stirring well, place in an ice bath at 0 °C. Slowly add 7.2 g of potassium permanganate in small amounts to the above mixed solution. After keeping the ice bath at 0°C and magnetic stirring for 3 hours, the obtained dark green suspension was transferred to a warm water bath at 50°C, and the temperature was controlled for 12 hours. The obtained purple-black suspension was slowly added to 160 mL ice-water mixture, and stirred vigorously for 1 hour. Then, 4 mL of 30% (mass concentration) hydrogen peroxide solution was added dropwise to the solution, and the color of the solution changed to bright yellow. The resulting solution was filtered through a G1 sand core funnel (pore size 20-30 microns), the filtrate was centrifuged at 4000 rpm for 30 minutes, and the sup...

example 2

[0041] 160 mg of polyallylamine hydrochloride and 160 mg of potassium hydroxide were sequentially added to 80 mL of 0.5 mg / mL graphene oxide aqueous solution, and the mixture was magnetically stirred in a water bath at 70 °C for 24 hours. The solution was collected and then centrifuged, washed with double distilled water until neutral, and excess polyallylamine hydrochloride and potassium hydroxide were removed. The obtained product was made up to 80 mL with double distilled water, and fully dissolved.

example 3

[0043] 40 mg of folic acid was first dissolved in 16 mL of sodium dihydrogen phosphate-disodium hydrogen phosphate buffer solution (pH 5, 20 mmol / L), and the 0.0227 mmol / L folic acid solution obtained after complete dissolution was mixed with 8 mL of 1-ethyl Base-3-(3-dimethylaminopropyl)-carbodiimide aqueous solution (0.068 mmol / L) and N-hydroxysuccinimide (0.068 mmol / L) mixed aqueous solution were mixed at room temperature in the dark Leave overnight to activate. Then fully mixed with the 80 mL polyallylamine hydrochloride-modified graphene oxide aqueous solution obtained in Example 2, and stirred magnetically at room temperature for 24 hours. After the reaction, the solution was collected and centrifuged by ultrafiltration, washed with double distilled water until neutral, and unbound folic acid and unreacted cross-linking agent were removed. The obtained product was made up to 80 mL with double distilled water, and fully dissolved to obtain a folic acid-graphene oxide aqu...

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Abstract

The invention discloses a folic acid-porous platinum-graphene oxide composite nano material as well as an application thereof in detecting tumor cells. The folic acid-porous platinum-graphene oxide composite nano material is prepared by the following steps: by taking graphene oxide as a substrate, carrying out amidation to crosslinked folic acid molecules; and carrying out in-situ synthesizing on platinum nano particles with porous structures. The folic acid-porous platinum-graphene oxide composite nano material can specifically identify a folate receptor on the surface of the cell, and meanwhile has a characteristic of simulating peroxidase and can catalyze hydrogen peroxide oxidized 3, 3', 5, 5'-tetramethyl benzidine hydrochloride to develop color. The number of the tumor cells can be quickly and agilely detected by means of chromogenic reaction, and 30 MCF-7 (Michigan Cancer Foundation-7) cells can be detected to the minimum extent. The material further can catalyze diaminobenzidine to generate a reddish brown precipitate on the surface of the cell so as to realize targeted positioning detection.

Description

technical field [0001] The invention relates to the preparation of folic acid-porous platinum-graphene oxide composite nanomaterials and a method for rapidly and sensitively detecting tumor cells, belonging to the fields of nanotechnology and medical testing. Background technique [0002] Despite advances in medical technology, cancer remains the deadliest disease in the world. The traditional detection and localization of tumors is difficult to achieve the expected detection effect due to the lack of methods to achieve targeted localization. The development of new methods for early diagnosis of cancer is critical to successfully treating cancer and increasing patient survival. [0003] Folic acid is a small molecular vitamin. Since scientists discovered in 1986 that folic acid enters cells through the receptor-mediated endocytic pathway, people have begun to conduct in-depth research on folic acid as a targeting carrier. It has been confirmed that folic acid receptors are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/574G01N33/558
Inventor 陈伟张灵娜邓豪华许雄伟李光文
Owner FUJIAN MEDICAL UNIV
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