81results about How to "Test results have no effect" patented technology

The invention discloses an electrochemiluminescence sensor for detecting trace pesticide residue and a method for detecting pesticide residue. An electrode preparation method (figure attached) comprises the following steps: selecting an identifier of the pesticide residue; preparing quantum dots and graphene nano material according to documents; and modifying the nano material, the quantum dots and the identifier onto the electrode surface of the sensor by the electrode surface modification technology. The method for detecting the trace pesticide residue comprises the following steps: connecting the modified electrode to an electrochemiluminescence instrument and detecting the pesticide residue in the sample extract. The electrode in the invention has high specificity and sensitivity capable of reaching ng level, a basic detection process is finished only within 3-5 min, and the cost is low. The method for detecting the pesticide residue by utilizing the electrode is fast and simple to operate, and the reaction is automatically finished and the result is automatically recorded by an apparatus.

The invention discloses a method for detecting an electrode of a molecular imprinting membrane of EDCs and the EDCs; the preparing method of the molecular imprinting membrane electrode comprises the following steps: a functional monomer is selected; template molecule of the EDCs, the functional monomer, a crosslinker, a porosity-making agent, an initiating agent and organic solvent are mixed evenly according to a certain molar ratio so as to prepare MIPs solution; nanometer material solution is prepared; the nanometer material and molecular imprinting polymer MIPs are modified on the surface of the electrode of a sensor by using the electrode surface modifying technology; a method for detecting trace amount of the EDCs comprises the following steps: the electrode of the molecular imprinting membrane is connected with an electrochemical working station and detects the EDCs in the environment sample extract; the electrode of the molecular imprinting membrane of the invention has strong specificity and high sensitivity which can reach ng level; a basic detection process is completed by only 1 to 2 minutes; the cost is low and the detection of one sample needs only a plurality of cents; the method for detecting the secretion in the environment by the electrode of the molecular imprinting membrane has fast and simple operation and the reaction and result are completed and recorded automatically by instruments.

The invention discloses a quantum dot molecular imprinting microsphere quartz fluorescent sensor for detecting multicomponent food additives simultaneously and a method for detecting the food additives by the quantum dot molecular imprinting microsphere quartz fluorescent sensor. A method for preparing a quantum dot molecular imprinting microsphere comb quartz plate comprises the following steps of: selecting functional monomers corresponding to the food additives; preparing quantum dots and preparing quantum dot molecular imprinting microspheres according to the literature; and modifying thesurfaces of different probes of the comb quartz plate with the imprinting microspheres of different food additives by utilizing layer-upon-layer accumulated surface modification technology. The method for detecting the trace multicomponent food additives simultaneously (which is shown as the figure) comprises the following steps of: immersing the modified quartz plate into simply-pulpified food solution, arranging the quartz plate on a sealing quartz vessel, and detecting the food additives in a sample. The quantum dot molecular imprinting microsphere quartz fluorescent sensor has high specificity and sensitivity, short detection time and low cost; and in a method for detecting pesticide residues by fluorescent light, the operation is simple and quick, and reactions and results are completed and recorded automatically by instruments.

The invention provides an endocrine interferent molecularly imprinted membrane substrate in a trace environment with rapid detection speed and high sensitivity, the preparation method and the application thereof. The molecularly imprinted membrane substrate comprises a gold quartz crystal substrate, and multi-layer nano-materials that alternate with each other and environmental endocrine interferent molecularly imprinted polymer are formed on the surface of the gold quartz crystal substrate. The preparation method comprises the following steps: selecting functional monomer that can be synthesized with the environmental endocrine interferent into the molecularly imprinted polymer; preparing the molecularly imprinted polymer solution; and modifying the nano-material and the molecularly imprinted polymer on the surface of the gold quartz crystal substrate. The invention applies the surface modification technology to the preparation of the molecularly imprinted membrane substrate, so as to allow the controllability over the preparation of the nano-scale synergetic EDCs molecularly imprinted membrane substrate. A method for detecting the environmental endocrine is rapid and simple, and the reaction and the result are automatically recorded and completed by an instrument with good repeatability, thereby facilitating the on-site detection.

The invention discloses a process for preparing a trace amount mycotoxin molecular imprinted column, which comprises the following steps of: selecting a functional monomer; evenly mixing a mycotoxin template molecule, the functional monomer, a cross-linking agent, a pore-forming agent, an initiator and an organic solvent by a certain molar ratio to prepare a molecular imprinting polymer solution; preparing a nano-solution; and dressing the nano-material and a molecular imprinting polymer on the internal surface of a glass pipe column. A method for detecting the trace amount mycotoxins includes: pumping a chemiluminescence system solution and a sample solution into a chemiluminescence analyzer respectively and detecting the mycotoxins in the sample. The molecular imprinted column of the invention has advantages of high sensitivity and accuracy. The nano-enhanced trace amount mycotoxin molecular imprinted column obtained in the invention is connected to the chemiluminescence analyzer for detecting mycotoxins, and purposes of high specificity, high sensitivity and fast detection to mycotoxins in the sample can be realized.

The invention belongs to the technical field of analytical chemistry and chemical sensors and discloses an electrochemical immunosensor for detecting a toxoplasma gondii IgM (Immunoglobulin m) antibody (Tg-IgM) of a gravida and a preparation method of the electrochemical immunosensor. The immunosensor is prepared by sequentially modifying graphene, polythionine, gold nanoparticles and capture antigen to the surface of a glassy carbon electrode. An enzyme-functionalized nano-composite detection probe with an electrical signal amplifying function is prepared by assembling enzyme and a second antibody with high proportions on an Au-Fe3O4 surface. According to the sandwich immunoassay principle, the concentration of Tg-IgM is determined by using an electrochemical signal generated by catalysis of enzyme to a substrate. According to the electrochemical immunosensor, the specificity of immunoreaction is combined with the sensitivity of electrochemical detection; the transmission of electronics is promoted by using the graphene, the polythionine, the gold nanoparticles, Au-Fe3O4 and other material; and the sensitivity of the detection is improved. The electrochemical immunosensor has the advantages of simplicity and convenience for operation, favorable regeneration performance and detection cost reduction. The electrochemical immunosensor prepared on the basis can be also used for detecting other immunological markers and has favorable application prospect in medical diagnosis.

The invention discloses a device and a method for measuring half-wave voltage of an electro-optic phase modulator. The device comprises a tunable laser device, a first optical coupler, a polarization controller, the electro-optic phase modulator to be detected, a second optical coupler, a photoelectric detector and a frequency spectrum analyzer which are connected in sequence and further comprises a microwave signal source and an acousto-optic frequency shifter. The device outputs optical carrier waves through the tunable laser device, divides the optical carrier waves into two beams through the first optical coupler, respectively processes the beams through the photoelectric detector and the electro-optic phase modulator to be detected, then combines the beams through the second optical coupler, achieves stable beat frequency, obtains the half-wave voltage of the electro-optic phase modulator by comparing the carrier wave beat frequency amplitude and the sideband beat frequency amplitude and has the advantages of high in resolution ratio, high in precision, good in accuracy, simple in structure and convenient to operate.

The invention relates to a flexible, simple and convenient loading device for an anti-seismic property test. The device comprises constraint units for fixing test pieces to ground beams, a horizontal loading unit for applying a horizontal load to the test pieces and a vertical loading unit for applying a vertical load to the test pieces; the vertical loading unit comprises vertical jacks, vertical flexible pull rods and reaction beams located over the test piece, the lower ends of the vertical flexible pull rods are fixed with the ground beams, the upper ends of the vertical flexible pull rods are connected with the reaction beams, and the vertical jacks are fixed to the bottoms of the reaction beams and apply vertical loads to the top of the test pieces. According to the device, the vertical flexible pull rods are thin and long rod parts with the certain flexibility, when the test pieces generate horizontal displacement deformation under the action of the horizontal load, the vertical flexible pull rod can generate the certain curvature, and therefore the upper ends of the vertical flexible pull rods can horizontally move along with the reaction beams and the vertical jacks, that is to say, the relative positions between the vertical loading unit and the test piece do not change. The device is simple in structure and easy to install.

The invention provides a multiplex fluorescent quantitative PCR primer and a probe for detecting H5, H7 and H9 subtype avian influenza viruses. With adoption of the primer and the probe, multiplex fluorescent quantitative PCR can be adopted to simultaneously detect which subtypes the H5, H7 and H9 subtype avian influenza viruses concretely are, no influences exist among the primers of different subtypes, the specificity is strong, the detection sensitivity is 10-50 copy numbers, a target sequence can be accurately detected quantitatively and qualitatively, the repeatability is good, and the reliability is high. The invention provides a multiplex fluorescent quantitative PCR kit for simultaneously detecting the H5, H7 and H9 subtype avian influenza viruses. The kit has the detection sensitivity being 10-50 copy numbers and is high in sensitivity and strong in specificity. The invention also provides a detection method which is good in stability. With adoption of plasmids with gene fusion as a positive standard substance, tedious operation of changing the positive standard substance for multiple times is avoided, the detection time is greatly shortened, the detection times are greatly reduced, and detection of a sample can be finished within 2h.

The present invention relates to a urine iodine determination kit. It includes urine purification device, diluent liquor, color-developing liquor, composite oxidant and standard colorimetric plate. Said kit can quickly remove interference substance from the urine, and can be used for accurately determining iodine concentration in the urine. The above-mentioned urine purification device is a titration type container in which a selective specific adsorbent for specifically adsorbing interference substance in the urine is held. Said selective specific adsorbent is made up by using purification active carbon and iodine adsorption agonist NaCl, MgCl2 or NaBr through baking activation process, the above-mentioned color-developing liquor is a mixed reagent formed from tetramethylbenzidine aqueous solution and polyethylene glycol. Besides, said invention also provides the composition of the composite oxidant.

The invention discloses a fluorescence nanocrystal quartz fluorescent sensor for molecular identification-based multicomponent simultaneous saccharide detection and a method for detecting saccharides by using the same. The invention provides a preparation method of a molecular identification-based fluorescence nanocrystal comb quartz plate, which comprises the following steps: selecting an identifier corresponding to the saccharides; preparing a fluorescence nanocrystal, and carrying out surface modification on the fluorescence nanocrystal according to the literature; and modifying the modified fluorescence nanocrystal onto surfaces of different probes of the comb quartz plate by using layer-by-layer accumulation surface modification technique. The invention also provides a method for multicomponent simultaneous saccharide detection, which comprises the following steps: immersing the modified quartz plate into a food sample solution which is simply pulpified, installing the quartz plate onto a sealed silica dish, and detecting the saccharides in the sample. The invention has the advantages of strong specificity, high sensitivity, short detection time and low cost. The saccharide fluorescence detection method has a quick and simple operation process, and the reaction and the result are automatically completed and recorded by instruments.

The invention discloses a method for quickly and quantificationally detecting live bifidobacterium and relates to a quick and quantificational detection method for the live bifidobacterium in products containing bifidobacterium. A detection technology of the bifidobacterium is divided into the prior detection with plate bacterial colony counting as a base and various detections with molecular biology as a base. The method comprises the following steps that: a sample is taken, is diluted up to 10 times, and is subjected to EMA treatment, DNA of the sample is extracted, the molecular beacon-real-time PCR detection is performed, the content of the bifidobacterium is calculated according to a standard curve, wherein, the extraction process of the DNA is that: the sample which is subjected to the EMA treatment is added with 18 percent sodium citrate and 1M NaOH and is centrifugated for 10min at a rotating speed of 10,000rpm, somatic cells are collected and are washed by ultrapure water, a bacterial suspension is taken, is added into a Tritox-100 liquid with a concentration of 2 percent, is subjected to water bath treatment for 10min at a temperature of 100 DEG C, and then is cooled immediately, and supernate is used for molecular beacon-real-time PCR amplification. The method is used for quantitative detection of the live bifidobacterium in the products containing the bifidobacterium.

The invention discloses a preparation method of a voltage-controllable multiplex multichannel sensing paper chip for detecting antibiotic residues by molecular imprinting electroluminescence. The preparation method comprises the following steps of self-preparing a transformer and a multiplexer switch, preparing a multichannel printing electrode on paper, preparing a molecular imprinting (MIPs) sol of antibiotic residues, preparing carbon dots and silicon dioxide pellets coated by the carbon dots, preparing a graphene nano-material, and modifying the surface of the multichannel printing electrode on paper by the graphene nano-material, the silicon dioxide pellets coated by the carbon dots and the MIPs sol by an electrode surface modification technology. The preparation method also comprises the following steps that through the modified multichannel printing electrode, a chemiluminescent analyzer, the transformer and the multiplexer switch, antibiotic residues in sample extract are detected. The voltage-controllable multiplex multichannel sensing paper chip has strong singularity, high sensitivity reaching the ng grade and a low cost, can be operated fast and simply, and realizes automatic reactions and result recording by apparatuses.

The invention relates to a method for measuring protection of a sunscreen cosmetic, which is characterized by comprising the following steps: a solution to be tested and a blank reference solution containing no cosmetic to be measured are prepared; then an integrating sphere is arranged between an absorption pond and a detector in an ultraviolet-visible spectrophotometer, the blank reference solution is employed for blank correction on the ultraviolet-visible spectrophotometer; the solution to be tested is added in the absorption pond of the ultraviolet-visible spectrophotometer finally, the solution to be tested is tested, the ultraviolet absorption spectrum of the solution to be tested is measured, and the cosmetic to be measured has better protection effect due to higher absorbance. According to the invention, the ultraviolet-visible spectrophotometer of the integrating sphere is increased for measuring the absorbance of the cosmetic to be measured, thereby the sunscreen effect of the cosmetic to be measured can be measured, the measurement method has the advantages of simple operation, low measurement cost, high credibility and accuracy, and easy popularization and application.

The invention discloses a method for preparing and using an electrochemical immunosensor for aflatoxin B1 detection, including preparation of an electrochemical immunosensors and alflatoxin B1 detection of the electrochemical immunosensor. According to the preparation scheme, the preparation method comprises the following steps: preparing a nano-gold solution; preparing an activated glassy carbonelectrode; preparing a graphene oxide / thionine / nano-gold compound material; preparing a modified glassy carbon electrode; sealing the non-specific site with bovine serum albumin; and preparing a detection substrate. The method realizes quick, accurate and low-cost detection on aflatoxin B1.

The invention discloses a label-free electrochemical immunosensor for detecting zearalanol and a manufacturing method thereof. The manufacturing method of the electrochemical immunosensor comprises the following steps: 1, preparing graphene; 2, preparing a mixed solution from thionine and graphene according to a certain molar ratio; and 3, modifying the electrode surface by the prepared mixed solution through utilizing an electrode surface modification technology, and modifying by an antibody. By connecting the electrochemical immunosensor to an electrochemical workstation, zearalanol in animal derived food extracts can be detected. According to the electrochemical immunosensor of the invention, the specificity is strong, and the sensitivity which is high can reach a pg grade; and only 1-2 minutes are needed to complete one basic detection process. The method is rapid and simple to operate, and the reaction and the result are automatically completed and recorded by an apparatus.

The invention relates to an active current extraction method based on orthogonal sine wave integral modulation. The method comprises the following steps: 1) a standard sine wave reference current is generated; 2) the integral modulation of the total power is performed; 3) the modulation power is obtained; and 4) the method for obtaining the active current can be realized by a computer or a hardware circuit and is as follows: the extraction method of active current signal or data is adopted, and the signals of the total current i and voltage u which are input in a load are utilized to obtain the active current signals ip through an algorithm below. The idle current and generalized idle current detected by the method of the invention do not contains any active component, the method does not contain filtering link; when the voltage is non-sine wave, phase-lock loop is not required and single-phase detection is performed directly and not influenced by the imbalance of the three phases in a circuit; and the detection result is not influenced by voltage harmonic and destortion, and the method is suitable for the real-time detection of current.

The invention relates to a glycosyl functional bacterial toxin molecularly imprinted membrane substrate as well as a preparation method and an application thereof. The molecularly imprinted membrane substrate forms a reaction layer on the surface of a gold quartz crystal substrate by taking a glycosyl functional bacterial toxin molecularly imprinted polymer as a recognition element. The preparation method comprises the following steps: selecting a carbohydrate molecule functional monomer which can carry out specificity recognition with bacterial toxin and compound the corresponding molecularly imprinted polymer; preparing a glycosyl functional molecularly imprinted polymer solution; and modifying the glycosyl functional molecularly imprinted polymer on the surface of the gold quartz crystal substrate by a substrate surface modifying technology to form a reaction layer. The glycosyl functional molecularly imprinted membrane substrate prepared by the method is connected to a piezoelectric quartz crystal microbalance to detect bacterial toxin in an environmental sample extracting solution. The molecularly imprinted membrane substrate has favorable molecular recognition performance and greatly improves the sensitivity and the selectivity for detecting the bacterial toxin.

The invention discloses an organic arsenide molecularly imprinted membrane substrate in aptamer-based marine products, and a production method and application thereof, wherein an organic arsenide molecularly imprinted polymer in the aptamer-based marine product is taken as a recognition element to form a reaction layer on the surface of a gold quartz body substrate; and the organic arsenide molecularly imprinted polymer in the aptamer-based marine product is formed by polymerizing organic arsenide template molecule, aptamer functional monomer, cross-linking agent, porogen, initiating agent and organic solvent in the marine product according to the molar ratio of (0.1-2.5):3:(0.1-3):(30-60):(0.01-0.15):(1.0-10). The surface finish technology is applied to the preparation of the organic arsenide molecularly imprinted membrane substrate in the aptamer-based marine product, so the preparation of the organic arsenide molecularly imprinted membrane substrate in the aptamer-based marine product has controllability, and the sensitivity and the accuracy of the substrate are improved.

The invention relates to a surface checking method of a printed circuit board. The surface checking method comprises the following steps of: performing laser scanning on the surface of the printed circuit board to obtain a three-dimensional structure of the surface of the circuit board; converting the three-dimensional structure of the surface of the printed circuit board into a two-dimensional planar image; and contrasting the two-dimensional planar image with a design configuration of the surface of the printed circuit board so as to determine defect information of the surface of the circuit board. With the method, even if the surface of the printed circuit board is oxidized or roughed, various defects of the surface of the printed circuit board also can be detected; during the checking process, detection parameters do not need to be adjusted according to the type of a base material of the printed circuit board; meanwhile, information such as copper layer thickness also can be obtained, and more data is provided for understanding a manufacturing technology of the printed circuit board better.

The invention relates to a method for preparing a glycosyl functional molecularly imprinted membrane electrode for detecting bacterial toxin, which comprises the following steps: (1) selecting a functional monomer which can compound a glycosyl functional molecularly imprinted polymer with the bacterial toxin; (2) uniformly mixing a bacterial toxin template molecule, the functional monomer, a cross-linking agent, a porogenic agent, an initiating agent and an organic solvent to prepare a glycosyl functional molecularly imprinted polymer solution according to a certain molar ratio; and (3) modifying the glycosyl functional molecularly imprinted polymer on the surface of an electrode of a sensor by an electrode surface modifying technology. The method for detecting trace bacterial toxin comprises the following steps: connecting the glycosyl functional molecularly imprinted membrane electrode prepared by the method to an electrochemical workstation and detecting the bacterial toxin in an environmental sample extracting solution. The glycosyl functional bacterial toxin molecularly imprinted membrane electrode has high specificity, sensitivity and detecting sped, can realize high flux screening of a plurality of samples in short time and reduces the detecting cost.

The invention relates to an immunity test strip based on a fluorescent microsphere for detecting an infectious pleural pneumonia antibody of a goat as well as a preparation and detection method, whichbelong to the detection field of immunity. The test strip comprises a shell and a test strip, wherein the test strip comprises a bottom plate as well as a water absorption pad, a detection pad, a combination pad and a sample pad which are successively lapped and adhered onto the bottom plate; a nitrocellulose membrane is provided with a quality control line C and a detection line T, the quality control line C covers a His tag monoclonal antibody, and the detection line T covers a goat mycoplasma pneumonia recombinant protein; the combination pad is a glass cellulose membrane coating with thegoat mycoplasma pneumonia recombinant protein marked by time resolution fluorescent microspheres; and the sample pad is a dry glass cellulose membrane after being soaked in sample treatment liquid. The test strip prepared by the invention has better stability and higher sensitivity, and can achieve a purpose of rapid semi-quantitative detection by virtue of the fluorescent signal analysis.

The invention relates to a sample preparation method used for determining abundance of a <15>N isotope of nitrite. The sample preparation method comprises the following steps: loading a sample, namelyloading a <15>N-labeled to-be-prepared sample and aqueous solution of a reducing agent into a first bulb of a double bulb reaction tube, and loading an acidic material into a second bulb of the double bulb reaction tube; preparing a <15>NO gas, namely maintaining vacuum degree, and adding the acidic material in the second bulb into the first bulb, so that the <15>NO gas is generated; detecting, namely after the vacuum degree of a pipeline between a vacuum plug valve and a gas isotope ratio mass spectrometer is reduced to be below 2*10<-5>Pa, opening the vacuum plug valve, and introducing the<15>NO gas in the first bulb into the gas isotope ratio mass spectrometer; setting detection parameters of the gas isotope ratio mass spectrometer, detecting a relative intensity when mass numbers are30 and 31, and performing a parallel test for multiple times; and calculating an abundance value of the <15>N isotope. Compared with the prior art, the sample preparation method provided by the invention has the advantages that an operation process is safe and reliable, sample pretreatment time is greatly shortened, economic cost is low and test data accuracy and precision are high.

The invention discloses a testing structure for improving failure analysis efficiency of breakdown voltage of an insulator. The testing structure comprises a plurality of shunt capacitors, wherein an upper polar plate of each capacitor is connected with a first metal pad through a first connecting wire, and a lower polar plate of each capacitor is connected with a second metal pad through a second connecting wire; and the testing structure is characterized by also comprising a third metal pad and a fourth metal pad, wherein the third metal pad is connected with the second connecting wire through a third connecting wire, and the fourth metal pad is connected with the first connecting wire through a fourth connecting wire. The testing structure for improving the failure analysis efficiency of the breakdown voltage of the insulator has the advantages that by adoption of the testing structure, the testing result is not influenced, and the failure positions of most of samples can be found during failure analysis.

The invention discloses a lanthanide metal europium ion complex probe-based method for detecting a pH value. The detection method mainly comprises the following steps: firstly, carrying out fluorescence intensity scanning on a to-be-detected sample A including lanthanide metal europium ion complexes and solutions with different pH values; observing the change of the fluorescence intensity, and carrying out quantitative detection on the pH values of the solutions, so as to obtain a linear interval between the pH values and the fluorescence intensity; and detecting the fluorescence intensity of a to-be-detected sample B including the lanthanide metal europium ion complexes and a to-be-detected water body sample, so as to obtain the pH value of the to-be-detected water body sample. The method disclosed by the invention is capable of achieving the detection effect of a traditional method, and has good selectivity, high response speed, short detection time, simplicity of operation and low price; a time-resolved fluorescence signal is strong and stable; and the repeatability of an experimental method and the recognizable degree of the fluorescence signal in a complicated environment are ensured, so that the ultimate practicability of the detection method is powerfully guaranteed.

The invention provides a solid coupling agent for a portable ultrasonic liquid level detector. The solid coupling agent is mainly composed of base glue and a filler. The solid coupling agent has goodelasticity, and can be obviously deformed under the action of an external pressure; and an air gap between a piezoelectric ultrasonic probe and tested equipment can be eliminated, so that the purposeof eliminating air layer blockage is achieved.

The invention relates to the field of environmental endocrine disruptors, and provides a kit for simultaneously detecting a plurality of environmental endocrine disruptors and application thereof. The kit is made by adopting the following steps: respectively preparing potassium permanganate solution, HCL solution and polysorbate-80 solution; uniformly mixing template molecules, a functional monomer, a cross-linking agent, a porogenic agent, an initiator and organic solvent in a certain molar rate to prepare molecularly imprinted sol-gel of the environmental endocrine disruptors; modifying the molecularly imprinted sol-gel on the inner surface of a microporous plate, sealing the inner surface by a preservative film for hours; soaking the modified microporous plate in eluant for eluting; and inserting ports of a sample injector of the microporous plate into luminescence reagent solution to obtain the kit for simultaneously detecting a plurality of environmental endocrine disruptors. The kit is connected to a multifunctional analyzer on the microporous plate to detect the environmental endocrine disruptors in an environmental sample extracting solution. The kit has the advantages of simple operation, less dosage of reagent, economy and practicality, low price and accessibility of chemical luminescent reagent, high sensitivity and simple operation.

The invention relates to a nanometer synergistic glycosyl group functionalized bacterial toxin molecular engram film substrate as well as a preparation method and an application thereof. The nanometersynergistic bacterial toxin molecular engram film substrate is to form a plurality of layers of mutually-alternating nanometer materials and glycosyl group functionalized bacterial toxin molecular engram polymers on a gold quartz crystal substrate by taking the glycosyl group functionalized bacterial toxin molecular engram polymers as coupling agents. The preparation method is realized as follows: selecting sugar molecule functional monomers which can recognize the specificity of bacterial toxin and synthesize corresponding molecular engram polymers; preparing molecular engram polymer solution; and modifying the nanometer materials and the molecular engram polymers on the surface of the gold quartz crystal substrate. The molecular engram substrate is connected to a piezoelectric quartz crystal microbalance to detect the bacterial toxin in an environment sample extraction solution. The invention has simple operation and high specificity and sensitivity for detecting the bacterial toxin.

The invention discloses a testing method of contact heat resistance between circular section one-dimensional nanostructures. The method comprises the following steps: breaking a circular section one-dimensional nanostructure into two sections by using a micro manipulator under an optical microscope or a scanning electron microscope, wherein two sections are respectively lapped at a heat source anda heat sink of an overhead microdevice; enabling two sections of specimens to form parallel contact between the heat source and the heat sink; testing the sample by adopting a heat bridge method, thereby obtaining apparent heat resistance Rtot1; changing two sections of specimens as cross contact from parallel contact by using the microdevice under the optical microscope or the scanning electronmicroscope, testing the sample by adopting the heat bridge method so as to obtain the apparent heat resistance Rtot2, wherein the contact heat resistance of the unit area between two sections of specimens can be approximate as RCA=(Rtot2-Rtot1)*Ac2, wherein the AC2 is the contact area of the cross contact between two sections of specimens. The testing successful rate is improved, the specimen quality consistency requirement is reduced, and the testing precision is improved.

The invention relates to a preparation method of a nano-synergistic carbohydrate functionalized molecularly imprinted column for detecting bacterial toxins, comprising the following steps: selecting a carbohydrate functional monomer which can be used for synthesizing a carbohydrate functionalized molecularly imprinted polymer with the bacterial toxins; preparing solution of the carbohydrate functionalized molecularly imprinted polymer; preparing nano solution; and modifying nano materials and the carbohydrate functionalized molecularly imprinted polymer on the inner surface of a glass tube column. A method for detecting trace bacterial toxins of the invention is characterized by connecting the carbohydrate functionalized molecularly imprinted column prepared by the above method with a chemiluminescence analyzer, respectively pumping chemiluminescence system solution and sample solution into the chemiluminescence analyzer and detecting the bacterial toxins in the samples. The preparation method of the invention has controllability and improves the sensitivity and accuracy of the column. The prepared molecularly imprinted column features strong specificity, high sensitivity and rapid detection speed, can realize high throughout screening of vast samples in short time and lower the detection cost.