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Preparation and application of electrochemiluminescence sensor for detecting trace pesticide residue

A pesticide residue, electrochemistry technology, applied in chemiluminescence/bioluminescence, electrochemical variables of materials, analysis by chemical reaction of materials, etc., can solve problems such as uncomfortable rapid detection, complex detection process, and inability to rapid detection. , to achieve the effect of high-throughput screening, fast and simple operation, and avoid the influence of subjective factors

Inactive Publication Date: 2011-03-30
UNIV OF JINAN
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  • Abstract
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Problems solved by technology

[0008] 3. The instrumental detection method for pesticide residues established with the help of large precision instruments such as HPLC or LC-MS combined technology has high sensitivity for the detection of pesticide residues, but this method cannot identify the analyte at one time structure, often need to use GC-MS to confirm the structure, the technical cost is high, the time required by the traditional method is generally long, some even as long as several hours, the operation is complicated, and cannot be used for rapid on-site detection
[0009] 4. For the detection and analysis of pesticide residues, the above methods generally have disadvantages such as low detection sensitivity, high cost, false positive results, complicated detection process, single detection, large amount of reagents, and not suitable for on-site rapid detection. Meet the needs of actual testing

Method used

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  • Preparation and application of electrochemiluminescence sensor for detecting trace pesticide residue
  • Preparation and application of electrochemiluminescence sensor for detecting trace pesticide residue
  • Preparation and application of electrochemiluminescence sensor for detecting trace pesticide residue

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preparation example Construction

[0038] A method for preparing an electrochemiluminescent sensor for detecting trace pesticide residues, characterized in that it comprises the following steps:

[0039] (1) select the identifier capable of interacting with pesticide residues;

[0040] (2) Use the prepared quantum dot material solution to increase efficiency, and combine carbon nanotubes or graphene to construct sensors;

[0041] (3) Using assembly modification technology, carbon nanotubes or graphene, quantum dot materials and recognition bodies are modified to the electrode surface to prepare electrochemiluminescent sensors.

[0042] The modification of the carbon nanotube or graphene, the quantum dot material and the target molecule recognition body in the present invention to the surface of the electrochemiluminescence sensor comprises the following steps:

[0043] (1) Grind the electrodes carefully with 0.05μm alumina powder, clean them with ultrasonic waves, and then wash them with 1mol / L HNO 3， 1mol / L ...

Embodiment 1

[0056] Embodiment 1 (organophosphorus, such as trichlorfon)

[0057] A method for preparing a molecularly imprinted membrane chemiluminescent sensor for detecting trichlorfon, comprising the following steps:

[0058] (1) Select p-sulfonic acid calix[4]arene that can interact with trichlorfon;

[0059] (2) Preparation of carbon nanotube solution: under the condition of ultrasonic stirring, 2 mg of multi-walled carbon nanotubes were added to 1 mL of dimethyl sulfoxide solution to obtain a black suspension, that is, a multi-walled carbon nanotube solution;

[0060] (3) Take 20 μL of the prepared multi-walled carbon nanotube solution, and sonicate for 30 minutes to obtain a uniformly dispersed multi-walled carbon nanotube solution;

[0061] (4) Preparation of CdTe quantum dot solution: in N 2 Under protection, the currently prepared NaHTe is used as Te precursor, and CdCl 2 Reaction, under the condition of thioglycolic acid as stabilizer, make water-soluble CdTe quantum dot sol...

Embodiment 2

[0069] Embodiment 2 (carbamate, as carbofuran)

[0070] A method for preparing an electrochemiluminescent sensor for detecting carbofuran, comprising the following steps:

[0071] (1) select the recognition body calix [8] arene sulfonate sodium that can interact with carbofuran;

[0072] (2) Preparation of graphene solution: under the condition of ultrasonic stirring, 2 mg of graphene was added to 1 mL of dimethyl sulfoxide solution to obtain a black suspension, namely graphene solution;

[0073] (3) Take 20 μL of the prepared graphene solution and ultrasonicate for 30 minutes to obtain a uniformly dispersed graphene solution;

[0074] (4) Preparation of CdSe quantum dot solution: under the protection of Ar gas, Se powder is dissolved in trioctylphosphorus to obtain Se solution, CdO is dissolved in oleic acid and octadecene, and the Se solution is injected to obtain the oil phase CdSe solution. The solution was added to ethanol, chloroform and thioglycolic acid to convert to...

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Abstract

The invention discloses an electrochemiluminescence sensor for detecting trace pesticide residue and a method for detecting pesticide residue. An electrode preparation method (figure attached) comprises the following steps: selecting an identifier of the pesticide residue; preparing quantum dots and graphene nano material according to documents; and modifying the nano material, the quantum dots and the identifier onto the electrode surface of the sensor by the electrode surface modification technology. The method for detecting the trace pesticide residue comprises the following steps: connecting the modified electrode to an electrochemiluminescence instrument and detecting the pesticide residue in the sample extract. The electrode in the invention has high specificity and sensitivity capable of reaching ng level, a basic detection process is finished only within 3-5 min, and the cost is low. The method for detecting the pesticide residue by utilizing the electrode is fast and simple to operate, and the reaction is automatically finished and the result is automatically recorded by an apparatus.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection, more specifically to the preparation of an electrochemiluminescence sensor for detecting trace pesticide residues, and the invention also relates to the use of the electrochemiluminescence sensor to detect traces in agricultural product samples. methods for measuring pesticide residues. Background technique [0002] Pesticide residues mainly include insecticides, fungicides, herbicides, etc. according to different uses of pesticides. Pesticide residues interfere with the normal behavior of organisms and the synthesis, storage, secretion, transport, combination, and clearance of normal hormones related to reproduction and development. [0003] At present, pesticide residues have become a new generation of environmental pollutants and food pollutants that urgently need to be treated. A small amount of pesticide residues can affect normal hormones, interfere with endocrine funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76G01N27/30
Inventor 葛慎光于京华颜梅黄加栋杨萍葛磊万夫伟赵珮妮高伟强卢娟娟王少伟张萌藏德进王寿梅闫纪宪
Owner UNIV OF JINAN
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