Kit for simultaneously detecting and tracing a plurality of environmental endocrine disruptors and application thereof

Abstract

The invention relates to the field of environmental endocrine disruptors, and provides a kit for simultaneously detecting a plurality of environmental endocrine disruptors and application thereof. The kit is made by adopting the following steps: respectively preparing potassium permanganate solution, HCL solution and polysorbate-80 solution; uniformly mixing template molecules, a functional monomer, a cross-linking agent, a porogenic agent, an initiator and organic solvent in a certain molar rate to prepare molecularly imprinted sol-gel of the environmental endocrine disruptors; modifying the molecularly imprinted sol-gel on the inner surface of a microporous plate, sealing the inner surface by a preservative film for hours; soaking the modified microporous plate in eluant for eluting; and inserting ports of a sample injector of the microporous plate into luminescence reagent solution to obtain the kit for simultaneously detecting a plurality of environmental endocrine disruptors. The kit is connected to a multifunctional analyzer on the microporous plate to detect the environmental endocrine disruptors in an environmental sample extracting solution. The kit has the advantages of simple operation, less dosage of reagent, economy and practicality, low price and accessibility of chemical luminescent reagent, high sensitivity and simple operation.

Description

technical field [0001] The invention relates to the technical field of detection of environmental endocrine disruptors, more specifically, a kit capable of simultaneously detecting trace amounts of various environmental endocrine disruptors, and the invention also relates to the use of the kit in detecting various environmental endocrine disruptors applications in interfering substances. Background technique [0002] Environmental endocrine disrupting chemicals (EDCs) refer to exogenous chemical substances that can interfere with the synthesis, secretion, transport, binding and metabolism of endogenous hormones in organisms that maintain their own balance and regulate developmental processes. Environmental endocrine disruptors are divided into the following categories: polychlorinated biphenyls, dioxins, alkylphenols, dibutyl phthalates, metals, organochlorine compounds, environmental hormones, phytoestrogens, Fungal estrogens are widely found in chemical products such as h...

AI Technical Summary

Problems solved by technology

[0005] 1. Uterine weight gain test and uterine calcium-binding protein CaBP-9k mRNA expression detection and protein analysis are often used in in vivo tests. This method is sensitive and specific, but its cost is high and its sensitivity is low, so it is not suitable for large-scale rapid screening. ;

[0006] 2. In vitro tests mainly include E-Screen method, receptor competition test, etc. The specificity of the test itself is poor, and false positive results are prone to occur;

[0007] 3. The EDCs instrument detection method established with the help of large precision instruments such as HPLC or LC-MS combined technology has high sensitivity for the detection of EDCs, but this method cannot identify the structure of the analyte at one time, often Need to use GC-MS to confirm the structure, the technical cost is high, the traditional method generally takes a long time, some even up to several hours, the operation is complicated, and cannot be used for rapid on-site detection

[0008] 4. For the detection and analysis of EDCs, the above methods generally have shortcomings such as low detection sensitivity, high cost, false positive results, complicated detection process, and not suitable for rapid on-site detection, so they cannot meet the needs of actual detection.

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