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Method for saccharification of lignocellulose by ultrasonic synergistic catalysis of modified cellulose

A cellulase and synergistic modification technology, applied in biochemical equipment and methods, enzymes, enzymes, etc., can solve the problems of difficulty in fully exerting enzyme catalysis, low efficiency of cellulase action, and complex lignocellulose structure. Achieve the effect of enhanced adaptability, reduced equipment cost, and reduced reaction time

Inactive Publication Date: 2007-05-30
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This process has the advantages of low energy consumption and no pollution, but due to the complex structure of lignocellulose, it is difficult to decompose; the action efficiency of cellulase is low, and its action efficiency mainly depends on the pretreatment process of raw materials; The pretreatment process needs to be developed, so improving the efficiency of enzyme catalysis and reducing the amount of enzyme to reduce the cost are the main problems in the current lignocellulosic saccharification process
The use of compound enzyme catalysis is an effective means to improve the efficiency of enzyme catalysis, but due to the differences in the use conditions of different enzyme preparations, the compound enzyme catalysis process is very complicated or it is difficult to fully exert the catalytic effect of the enzyme

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1: Corn stalks were crushed into 40-mesh particles, 10 g of crushed raw materials were taken, 150 ml of 8% NaOH was added and reacted at 25° C. for 8 hours, and the filter residue and filtrate were obtained by suction filtration. The pH value of the filtrate was adjusted to 3 with 6.5ml of 98% concentrated sulfuric acid, the temperature was 50°C, and the precipitation was performed for 30 minutes by centrifugation to obtain 0.92g of pure lignin with an extraction rate of 84.4% and a product purity of 82%. Wash the filter residue with water until the pH value is 8, then add 80mlH to the filter residue 2 O, adjust the temperature to 50°C, and adjust the pH value to 6.5 with 1.2ml of 2mol / L sulfuric acid.

[0013] Dissolve 20.0 g of cyanuric chloride in 300 mL of benzene, and recrystallize twice at 20° C. to obtain 17.9 g of purified cyanuric chloride. Add 5.0g of 4A molecular sieves, 10.0g of anhydrous sodium carbonate, 500mL of benzene and 20.0g of monomethoxypo...

Embodiment 2

[0015] Example 2: Corn stalks were crushed into 20-mesh particles, 10 g of crushed raw materials were taken, 150 ml of 5% NaOH was added to react at 25° C. for 3 h, and the filter residue and filtrate were obtained by suction filtration. The pH value of the filtrate was adjusted to 3 with 4.2ml of concentrated sulfuric acid with a mass concentration of 98%. The temperature was 50° C., and the precipitate was centrifuged for 30 minutes to obtain 0.73 g of pure lignin, with an extraction rate of 67% and a product purity of 79%. Wash the filter residue with water until the pH value is 8, then add 80mlH to the filter residue 2 O, adjust the temperature to 50°C, and adjust the pH value to 6.5 with 1.1ml of 2mol / L sulfuric acid.

[0016] Dissolve 10.0 g of cyanuric chloride in 150 mL of benzene, and recrystallize twice at 12°C to obtain 8.7 g of purified cyanuric chloride. Add 2.5g of 4A molecular sieves, 8.0g of anhydrous sodium carbonate, 400mL of benzene and 10.0g of monomethoxy...

Embodiment 3

[0018] Example 3: crush corn stalks into 40-mesh particles, take 10 g of crushed raw materials, add 120 ml of 3% NaOH, react at 25° C. for 3 hours, and filter with suction to obtain filter residue and filtrate. The pH value of the filtrate was adjusted to 6 with 3.6ml of concentrated sulfuric acid with a mass concentration of 98%. The temperature was 30° C., and the precipitate was centrifuged for 30 minutes to obtain 0.66 g of pure lignin with an extraction rate of 60.6% and a product purity of 72%. The filter residue was washed with water until the pH value was 8, and then 80ml of H was added to the filter residue. 2 O, adjust the temperature to 50°C and the pH to 6.5.

[0019] Dissolve 20.0 g of cyanuric chloride in 300 mL of benzene, and recrystallize twice at 3° C. to obtain 18.3 g of purified cyanuric chloride. Add 8.0g of 4A molecular sieves, 12.0g of anhydrous sodium carbonate, 600mL of benzene and 30.0g of monomethoxypolyethylene glycol 4000 into a 1000mL three-necke...

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PUM

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Abstract

The invention discloses a saccharifying method of xylon cellulose through ultrasonic coordinated modified cellulose enzyme, which comprises the following steps: grinding and predisposoing xylon in the raw material through alkaline; reacting activated methoxy carbowax and cellulose enzyme in the citrate-sodium citrate buffer solution to obtain modified cellulose enzyme; blending modified cellulose enzyme, beta-glucosidase, amylase and pectase to obtain composite liquid; adding composite enzyme into predisposed raw material according to corresponding proportion; proceeding enzyme catalytic reaction through ultrasound; filtering; decompressing; evaporating; obtaining condensed sugar liquid.

Description

technical field [0001] The invention relates to a method for catalyzing lignocellulose saccharification by ultrasonic synergistically modified cellulase. It belongs to the technology of lignocellulose saccharification. Background technique [0002] The production process of fuel ethanol can be divided into two processes, namely the saccharification of raw materials and the fermentation of sugar, that is, the process of converting raw materials into fermentable sugars and the process of using sugars for microbial fermentation to produce ethanol. Throughout the production process, biocatalytic technology is widely used. The production of traditional fuel ethanol uses grain as raw material, mainly uses amylase as a catalyst to convert starch into fermentable sugars, and its output is limited by grain resources, making it difficult to meet energy demand for a long time. Biomass waste containing lignocellulose is another source of raw materials for t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12N9/42
Inventor 张裕卿梁江华李滨县
Owner TIANJIN UNIV
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