Method for pretreating lignocellulose by using renewable ionic liquid aqueous solution
A technology of lignocellulose and ionic liquid, which is applied in the field of lignocellulose utilization and chemical industry application, can solve the problems of reducing the economic feasibility of the pretreatment process, stirring, filtering, centrifuging difficulties, reducing the yield of reducing sugar, etc., and achieves super Strong water resistance, lower system viscosity, lower energy consumption
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Embodiment 1
[0027] 50wt%[choline][glycine] aqueous solution pretreatment of rice straw
[0028] a) Pretreatment: Accurately weigh 150mg of rice straw powder (150~350μm) and 3g of [choline][glycine] ionic liquid aqueous solution (the content of ionic liquid is 50wt%; Synthesized by the method described above), put them together in a 15mL Erlenmeyer flask, and stirred at 90°C for 12 hours under the protection of nitrogen; then cooled to room temperature, added 1 times the volume of the pretreatment solvent to dilute with deionized water, filtered, and then used 3 times the volume of deionized water to wash the filter residue 5 times, and the filter residue was dried to obtain the pretreated rice straw.
[0029] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U of Trichoderma reesei Cellulase (purchased from Sigma-Aldrich China Branch) was sealed and placed in a ...
Embodiment 2
[0031] 50wt%[choline][tryptophan] aqueous solution pretreatment of rice straw
[0032] a) Pretreatment: Accurately weigh 150 mg of rice straw powder (150–350 μm) and 3 g of [choline][tryptophan] ionic liquid aqueous solution (50 wt% ionic liquid content), put them together in a 15 mL Erlenmeyer flask, and Under protection, stir at 90°C for 12 hours; then cool to room temperature, add 2 times the volume of pretreatment solvent with deionized water to dilute, filter, then wash the filter residue with 3 times the volume of deionized water for 5 times, and the filter residue is dried That is, the pretreated rice straw is obtained.
[0033] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U of Trichoderma reesei Cellulase, after being sealed, is placed in a constant temperature oscillator at 200r / min and 50°C for reaction. After 12 hours, 200 μL was sam...
Embodiment 3
[0035] 50wt%[choline][phenylalanine] aqueous solution pretreatment of rice straw
[0036] a) Pretreatment: Accurately weigh 150mg of rice straw powder (150-350μm) and 3g of [choline][phenylalanine] ionic liquid aqueous solution (50wt% ionic liquid content), put them together in a 15mL Erlenmeyer flask, Under the protection of nitrogen, stir at 90°C for 12 hours; then cool to room temperature, add 1 times the volume of pretreatment solvent with deionized water to dilute, filter, then wash the filter residue with 3 times the volume of deionized water for 5 times, and dry the filter residue After that, the pretreated rice straw is obtained.
[0037] b) Enzymolysis: Accurately weigh 20 mg of the above-mentioned pretreated rice straw, place it in a 50 mL Erlenmeyer flask, add 7 mL of citrate buffer (50 mmol / L, pH 4.8) and 245 U of Trichoderma reesei Cellulase, after being sealed, is placed in a constant temperature oscillator at 200r / min and 50°C for reaction. After 12 hours, 200...
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