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Method of ionic liquid cosolvent effect reinforced enzymatic synthesis of isoquercitrin

An ionic liquid and isoquercitrin technology, applied in the direction of fermentation, can solve the problems of low solubility of rutin and poor enzymatic reaction efficiency, and achieve the effects of good application prospect, increased reaction rate and simple operation.

Inactive Publication Date: 2013-01-16
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the enhanced enzymatic synthesis of isoquercitrin using ionic liquids as co-solvents
[0004] In the traditional reaction system using buffered aqueous solution as the medium, the solubility of rutin is low, and the enzymatic reaction efficiency is poor

Method used

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  • Method of ionic liquid cosolvent effect reinforced enzymatic synthesis of isoquercitrin
  • Method of ionic liquid cosolvent effect reinforced enzymatic synthesis of isoquercitrin
  • Method of ionic liquid cosolvent effect reinforced enzymatic synthesis of isoquercitrin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Prepare 0.26g / L rutin mother liquor with pH=3.0 with disodium hydrogen phosphate-citric acid buffer solution, and use 5% ionic liquid [Emim][BF 4 ], the ratio of anion and cation is 1:1, 90% rutin mother solution and 5% hesperidin enzyme solution constitute the reaction system, and the reaction system is shaken at 300r / min for 1h at 60°C. After the reaction, rutin, isoquercitrin and quercetin were detected by HPLC. The conversion rate of rutin, the molar yield of isoquercitrin and the molar yield of quercetin were 30%, 23% and 0.5% respectively. Compared with the buffer system under the same conditions, the molar yield of isoquercitrin An increase of 0.8%.

Embodiment 2

[0026] Prepare 0.41g / L rutin mother solution with pH=10.0 with disodium hydrogen phosphate-citric acid buffer solution, and use 50% ionic liquid [Bmim][BF 4 ], the ratio of anion and cation is 1:1, 45% rutin mother solution and 5% hesperidin enzyme solution constitute the reaction system, and the reaction system is shaken at 50r / min for 40h at 20°C. After the reaction, rutin, isoquercitrin and quercetin were detected by HPLC. The conversion rate of rutin, the molar yield of isoquercitrin and the molar yield of quercetin were 30%, 15% and 1% respectively. Compared with the buffer system under the same conditions, the molar yield of isoquercitrin increased 2%.

Embodiment 3

[0028]Prepare 0.82g / L rutin mother liquor with pH=5.0 with disodium hydrogen phosphate-citric acid buffer solution, and use 10% ionic liquid [Emim][BF 4 ], the ratio of anions and cations is 1:1, 72% rutin mother liquor and 18% hesperidin enzyme solution constitute the reaction system, and the reaction system is shaken at 120r / min for 7h at 40°C. After the reaction, rutin, isoquercitrin and quercetin were detected by HPLC. The conversion rate of rutin, the molar yield of isoquercitrin and the molar yield of quercetin were 94%, 96% and 11% respectively. Compared with the buffer system under the same conditions, the molar yield of isoquercitrin increased 89%.

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Abstract

The invention relates to a method of ionic liquid cosolvent effect reinforced enzymatic synthesis of isoquercitrin. The method comprises a reaction system for synthesizing isoquercitrin by hydrolyzing rutin with a hesperidin enzyme or a naringin enzyme. The reaction system comprises a rutin mother liquid prepared by a disodium hydrogen phosphate-citrate buffer solution and a hesperidin enzyme liquid or a naringin enzyme liquid. The reaction system contains an ionic liquid formed by cations and anions. In the ionic liquid cosolvent system, the prepared isoquercitrin by hydrolyzing rutin under enzyme catalysis has increased reaction rate and higher yield than that in a conventional aqueous solution system, so that the ionic liquid cosolvent system provides a novel reaction medium for the production of isoquercitrin. The method is simple in operation and mild in reaction conditions, is environment-friendly, and has good application prospects.

Description

technical field [0001] The invention belongs to the field of enzymatically synthesizing food additives, and in particular relates to a method for enzymatically synthesizing isoquercitrin by ionic liquid co-solvent effect enhancement. Background technique [0002] Prior art: Isoquercitrin (Isoquercitrin), that is, quercetin-3-O-β-D-glucoside (Quercetin-3-glucose), is a very rare in nature with significant antioxidant, Anti-tumor and other biologically active flavonoids are advanced food additives, auxiliary drugs or active ingredients of drugs developed by major international pharmaceutical and food companies. Toxicology, 2010, 268(3): 213-218). Isolation and extraction of isoquercitrin from natural plants usually requires the use of a large amount of organic solvents, which is time-consuming and laborious, and the production efficiency is low. A large amount of acidic wastewater produced by acid hydrolysis seriously pollutes the environment, and quercetin and isoquercitrin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P17/06
Inventor 王俊吴福安孙国霞
Owner JIANGSU UNIV OF SCI & TECH
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