80 results about "Radioimmunoassay" patented technology

Double-stranded cDNA was synthesized from nucleic acid extracted from Norwalk virus purified from stool specimens of volunteers. One clone was isolated from a cDNA library constructed in a pUC-13 vector after amplification of the cDNA. The specificity of this cDNA (pUCNV-953) was shown by hybridization assays. The cDNA reacted with post (but not pre-) infection stool samples from Norwalk volunteers and with highly purified Norwalk virus, but not with other common enteric viruses such as hepatitis A virus and rotavirus. Finally, the probe detected virus in the same fractions of CsCl gradients in which viral antigen was detected using a specific Norwalk virus radioimmunoassay, and particles were detected by immune electron microscopy. Single-stranded RNA probes derived from the DNA clone after subcloning into an in vitro transcription vector were also used to show that the Norwalk virus contains a ssRNA genome of about 8 kb in size. The original clone was also used to detect additional cDNAs which represent at least 7 kb of nucleic acid of the Norwalk genome. The availability of a Norwalk-specific cDNA and the first partial genome sequence information allow rapid cloning of the entire genome and of establishment of sensitive diagnostic assays. Such assays can be based on detection of Norwalk virus nucleic acid or Norwalk viral antigen using polyclonal or monoclonal antibodies to proteins expressed from the cDNA or to synthetic peptides made based on the knowledge of the genome sequence. Assays using proteins deduced from the Norwlk virus genome and produced in expression systmes can measure antibody responses. Vaccines made by recombinant DNA technology are now feasible.

A method the direct analysis of an analyte in keratinized structures, e.g., hair and fingernails, which comprises preparing a mixture containing a low redox potential activator compound such as dithiothreitol or dithioerythritol, an enzyme suitable for the digestion of the keratin structure, a sample of the keratin structure and a biological detergent that aids the digestion of the keratinized structure at a relatively low pH, e.g., between about 6.2 and 8; permitting the enzyme to at least substantially digest the sample of keratin structure, and subjecting the digest solution to analysis, preferably by radioimmunoassay, to determine the identity and amount of analyte in the keratin structure sample. To accelerate the method, cupric sulfate may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention. The preferred biological detergents include betaine, sulfo-betaine, alkylglucosides and bile acids.

The present invention is directed to a new bone marrow stromal stem cell (BMSSC) marker, CD18, for use in selecting a population of cells enriched in BMSSCs, from bone marrow cells, adipose cells, or peripheral blood. The invention is further directed to methods for selecting a population of cells enriched in BMSSCs based on the selective expression of CD18 on their surface, using techniques known in the art such as fluorescent assisted cell sorting, an immunomagnetic method, flow microfluorimetry, immunofluorescence, immunoperoxidase staining, radioimmunoassay and immunoaffinity chromatography. The invention is further directed to the BMSSCs isolated based on CD18 expression, and their use to treat various diseases. In one aspect, the HMSSCs are transformed with a vector having a normal gene for CD18, and the transformed BMSSCs are administered to treat bone degenerative diseases and diseases of bone involving abnormal expression of CD18 expression of CD18.

The present invention discloses a method for researching the role of melatonin in predicting the outcome of ovarian reserve and in vitro fertilization-embryo transfer (IVF-ET), including: collecting basic clinical data of 61 women, and according to their ovary response to stimulation, mainly by age , the number of retrieved eggs, and the value of anti-Müllerian hormone were divided into three groups: ovarian low response group, normal group and high response group. The follicular fluid of 61 women was collected, the melatonin concentration in the follicular fluid of 61 women was measured by melatonin radioimmunoassay, and the melatonin concentration and other clinical data were analyzed in the ovarian low response group, normal group and high response group. level; analyze the relevant data of in vitro fertilization outcome in ovarian low response group, normal group, and high response group, and verify the correlation between melatonin concentration and in vitro fertilization-embryo transfer outcome; verify the relationship between melatonin level and ovarian Relevance of reserves. The invention can predict the outcome of ovarian reserve and in vitro fertilization-embryo transfer by detecting the melatonin concentration in the follicular fluid.

The invention discloses a detection method in the technical field of biological engineering, in particular a method for diagnosing cardiac troponin I (cTn I) in a semi-quantitative mode by employing double-indicatrix immunochromatography. Acute myocardial infarction (AMI) is a serious disease in a coronary heart disease, is a common emergency among middle-aged and elderly people, and is high in death rate. According to statistics, one third to a half of patients die before being sent to a hospital. In the next 20 years, the AMI is a primary cause for death of the patients around the world. The cTn I is a reliable index which is currently acknowledged and has the highest specificity and the longest duration for myocardial infarction diagnosis. At present, cTn I detection methods which are commonly used in clinic comprise an enzyme-linked immunosorbent assay method and a radioimmunoassay method, which are time-saving, labor-saving, difficult to popularize and high in cost. An immune colloidal gold technology is quickly developed in recent years and can be widely applied to detection of communicable diseases, early pregnancy and cancers. By employing colloidal gold immunochromatography, a quick detection method for the cTn I is established. By adoption of the method, a cTn I gray area of 1 to 5 ng / ml can be subjected to semi-quantitative detection.

A radioimmunoassay method for determining the quantity of an analyte of interest in a sample is disclosed. The analyte of interest may be an antigen or other chemical entity. A known antibody to the antigen or other entity is employed and is conjugated to a functionalized nanoparticle. Because of the high surface area presented by the present nanoparticle—antibody conjugates, the present radioimmunoassay method is particularly suited for the qualitative and quantitative analysis of low molecular weight chemicals.

The present invention relates to the serological detection of animals inoculated with Neospora by reacting the test serum with a protein reactive with Neospora. The protein may be a full-length natural or recombinant Neospora or T. gondii bradyzoite fusion protein or a truncated fusion protein or a fragment thereof. The protein can be used in any of a number of assays including enzyme-linked immunosorbent assay (ELISA), radioimmunoassay (RIA), Western blot and other suitable forms of immunoassay.

This invention relates to new safe plant drug, which contains Berberine and Baicalin (BB), inhibits carcinogenic and mutagenic action, lowers tyrosine kinase and decreases synthesis of DNA, RNA and protein of cancer cells. Also, the present invention proved a new radioimmunoassay (RIA) method for precise determination of Berberine and Baicalin. The RIA is an efficient analytical method for large clinical programs including double blind analysis (DBA), and good clinical practice (GCP).