345results about How to "Decreased fluorescence intensity" patented technology

The invention discloses a cucurbit [7] uril [3] rotaxane as well as a preparation method and application thereof, and belongs to the technical fields of organic synthesis and analytical chemistry. A fluorescence probe cucurbit [7] uril [3] rotaxane prepared by the method can be applied to high-selectivity and high-sensitivity detection of lysine or arginine or a mixture of the lysine and the arginine. Under the medium condition of an aqueous solution, a trace amount of lysine or arginine or the mixture of the lysine and the arginine can be detected by taking cucurbit urils supramolecular self-assembly as a probe system by adopting a fluorescence emission spectrophotometry at high sensitivity and high selectivity. The invention also discloses a preparation method and a technological condition of the cucurbit [7] uril [3] rotaxane. The reacting fluorescence excitation wavelength of the cucurbit [7] uril [3] rotaxane and the lysine or the arginine or the mixture of the lysine and the arginine is 317nm; and the maximal emission wavelength is 375nm.

The invention discloses a base fluorescence image capturing system device for high-flux genome sequencing. During the genome sequencing process, a to-be-tested DNA fragment sample is placed into a gene chip of a genome sequencer. The base fluorescence image capturing system device comprises a laser control unit and an image capturing unit, wherein the laser control unit is used for controlling parallel linetype laser beams in the Y direction and output by a laser light source to scan the gene chip in X direction and Y direction, and exciting a fluorochrome group carried by nucleotide in the to-be-tested DNA fragment sample to give out light; the image capturing unit is used for separating light emitted by the fluorochrome group so as to obtain a plurality of kinds of light with different wave lengths, and imaging the light by utilizing a TDI camera module. The invention further provides a method utilizing the base fluorescence image capturing system device to capture images, which comprises the following steps: controlling a scanning galvanometer to carry out linear array scanning, converting the optical information into electric signals by utilizing a photovoltaic conversion method as to generate image information finally, and processing through an image processing algorithm to obtain the sequence of the bases of the DNA fragment. With the adoption of the device and the method provided by the invention, high-flux sequencing of the DNA fragment is satisfied, and meanwhile, the device and the method have the advantages of safety and stability, and high degree of automation.

Visible-light response hybrid aerogel and a preparation method and application thereof in waste gas processing are disclosed. Dicyandiamide is taken as a precursor and is calcined in two times to prepare a carbon nitride nanosheet; the carbon nitride nanosheet is dispersed in water, silver metavanadate quantum dots are subjected to in-situ growth to prepare a silver metavanadate quantum dot / carbon nitride nanosheet composite material; the silver metavanadate quantum dot / carbon nitride nanosheet composite material and graphene oxide carry out hydrothermal reaction, and are then frozen and dried to prepare silver metavanadate quantum dot / carbon nitride nanosheet / graphene hybrid aerogel which is the visible-light response hybrid aerogel. The problems of large reduction dosage, serious secondary pollution, complexity in operation and the like generated when waste gas is processed by a traditional flue gas denitration technology are overcome.

The invention discloses an imidazole [1,5-a] pyridine-rhodamine hydrazide derivative Cu<2+> ratio fluorescence probe.The probe is imidazole [1,5-a] pyridine substituted rhodamine hydrazide, a chemical structural formula of the probe is shown as a formula (1).The fluorescence probe has the unique fluorescence selectivity to Cu<2+> in an ethanol solution, higher sensitivity and stronger other ion interference capability and has a great application prospect.

The invention relates to carbonyl terminated hyperbranched polycarbonate capable of emitting bright fluorescence. Glycerol and diethyl carbonate are used as raw materials; a simple and controllable ester exchange condensation method is used for synthesizing the terminated hyperbranched polycarbonate; then, tert-Butyl acetoacetate is used for termination to obtain the hyperbranched polycarbonate. The synthesized hyperbranched polycarbonate does not contain pi keys such as benzene rings; the addition of fluorescent powder is not needed; the bright blue fluorescence can be given out; the characteristics of simple process, controllable process and low three-waste pollution are realized; in addition, the product stability is high; the toxicity is high; the biodegradability is high; the light intensity is high; the application range is wide.

The invention discloses a fluorescent probe and a pesticide residue detection kit based on a carboxylesterase inhibition method. The pesticide residue detection kit comprises a reagent stock solution a and a reagent stock solution b, wherein the reagent stock solution a is a buffer solution containing phosphate, and the reagent stock solution b is a solution of the fluorescent probe. Experiments find that the strength of the self fluorescence of the probe is very weak, and after carboxylesterase is added, the probe react and release an IR-780 hemicyanine skeleton fluorescent parent body, the fluorescence of the solution is remarkably strengthened, showing that the method can be used for detection of the activity of the carboxylesterase; after adding a tiny amount of pesticides in the carboxylesterase, the fluorescence of the solution is remarkably weakened, showing that a tiny amount of the pesticides can inhibit the activity of the carboxylesterase, and thus the fluorescent probe can be used for detection of pesticide residues. The fluorescent probe has the advantages of being easy to operate, low in cost, fast, efficient and sensitive, and the fluorescent probe is convenient for application and popularization, and has a huge application prospect in the fields of agriculture, food and the like.

The invention discloses an up-conversion fluorescent aptamer-based side stream test paper detection method, and belongs to the technical fields of environment detection and food safety detection. A nucleic acid aptamer capable of specifically identifying target bacteria is modified on the surface of each nano-gold particle; cDNA which is in complementary pairing with the aptamer is modified on thesurface of each rare earth up-conversion fluorescent particle; and after the two kinds of nanoparticles are mixed, the basic groups of the two segments of nucleic acid sequences are in complementarypairing and the distance of the two kinds of particles are pulled closely, so that fluorescence of the upper-conversion fluorescent particles is quenched. After a target object is added, the target object can be bound with the aptamer with cDNA competitiveness, so that fluorescence recovery of the up-conversion nanoparticles is caused. According to different fluorescence recovery intensity, quantitative measurement of the target bacteria can be realized. The method provided by the invention is high in sensitivity, high in specificity and simple and convenient in operation, various target objects can be measured through change of the aptamer sequence, and very import significance in the aspects of environment monitoring, food analysis and the like is achieved.

The invention discloses a method for improving the photocatalytic activity of bismuth tungstate through an excessive Bi source. Through adjusting the raw material mole ratio of Bi (NO3) 3.5 H2O and Na2WO4.2 H2O, a series of photocatalysts with different mole ratios of Bi / W are prepared by using a simple hydrothermal method implemented without any organic molecule additives, and the performances of the photocatalysts on playing a catalytic oxidation role on dye rhodamine B under simulated sunlight irradiation are studied. Results show that when the Bi source is excessive, the performances of the photocatalysts are improved at different levels; and X-ray diffraction results show that when the Bi source is excessive, besides a Bi2WO6 matrix, trace bismuth subnitrate Bi6O6 (OH) 3 (NO3) 3.1.5 H2O also can be obtained, and the appearance of a sample is integrally of a sheet hypersphere structure, but the rigidity and the assembling density of a sheet layer are higher than those of pure Bi2WO6, and the thickness of the sheet layer is obviously thinner than that of the Bi2WO6 by 15-30nm. Fluorescence tests show that the recombination probability of a photon-generated carrier of a sample with the excessive Bi source is really and effectively inhibited.

The invention discloses preparation and application of an ATP fluorescent probe for positioning mitochondria, wherein the probe has the structural formula defined in the specification and is synthesized by rhodamine B, diethylenetriamine, 5-bromo-valeric acid and triphenylphosphine as raw materials. In the system, ATP induces ring opening of a rhodamine lactam structure, strong fluorescence is produced, and high sensitivity is showed on ATP detection. At the same time, the probe shows good selectivity on the ATP, and is hardly interfered by other biological anions or inorganic anions. When the pH value is 6.0-8.0, ATP determination of the probe is not affected. In addition, the probe can be positioned in the mitochondria, and changes of the content of the ATP in the mitochondria are real-timely monitored.