The invention discloses a hepatitis E virus IgG antibody detection kit. The kit includes the following components: a solid material coated with a hepatitis E virus (HEV) gene recombinant antigen, a mouse anti human IgG monoclonal antibody labeled with a signal generation substance, a concentrated washing solution, an enhancing liquid, an analysis buffer liquid, a sample diluent, a positive control and a negative control. In addition, the invention also provides a preparation method of the kit. The preparation method includes the following steps: a first step, preparing the solid material coated with the HEV gene recombinant antigen; and a second step, labeling a mouse anti human IgG monoclonal antibody with the signal generation substance. In addition, the invention also discloses an application of the kit in detection of a hepatitis E virus IgG antibody. The detection kit overcomes the defect of labeling antibodies with macromolecules (such as enzymes), has the advantages of high sensitivity, good stability, low cost and the like, can significantly improve the specificity, sensitivity and stability of detection of the hepatitis E virus IgG antibody, and besides, significantly reduces the cost.