Immunochromatographic test strip for rapidly detecting malaria and preparation method thereof

A technology for immunochromatography and test strips, applied in the field of medical testing, can solve the problems of inability to display test results in multiple styles, difficult to distinguish test lines and quality control lines, and complicated preparation process of colloidal gold labels, so as to improve sensitivity and test results. accuracy, improved preventive capabilities, and significant economic and social benefits

Active Publication Date: 2011-06-08
GUANGZHOU WONDFO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its defect is that it is impossible to carry out joint inspection of falciparum malaria and non-falciparum malaria
At present, the development of immunochromatography technology using nano-colloidal gold as a marker is very mature, but it has a certain gap in sensitivity and stability compared with the Elisa method or PCR method, and the preparation process of colloidal gold labeling is complicated and the cost is high
And because colloidal gold can only display red, it cannot display test results in multiple styles, especially for multi-item test strips, it is difficult to distinguish the test line from the quality control line, and it is easy to cause misjudgment of the test results

Method used

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  • Immunochromatographic test strip for rapidly detecting malaria and preparation method thereof
  • Immunochromatographic test strip for rapidly detecting malaria and preparation method thereof

Examples

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Embodiment 1

[0032] see figure 1 , is a structural schematic diagram of the malaria immunochromatographic rapid detection test strip of the present invention. A test strip for rapid detection of malaria falciparum and non-falciparum malaria provided by the present invention comprises a substrate 1 , a sample pad 2 , a marking pad 3 , a coating film 4 and absorbent paper 5 . Marker pad 3 coated with colored latex-labeled Plasmodium falciparum histidine-rich protein II (HRP-II) monoclonal antibody and non-Plasmodium falciparum lactate dehydrogenase (pLDH) monoclonal antibody, coated membrane 4 by the detection zone 6 and the control area 7, the detection area 6 includes the detection line T1 and the detection line T2, wherein T1 is coated with colored latex-labeled Plasmodium falciparum histidine-rich protein II (HRP-II) monoclonal antibody in different surface Another strain of Plasmodium falciparum histidine-rich protein II (HRP-II) monoclonal antibody; T2 is coated with a non-Plasmodium ...

Embodiment 2

[0044] The detection test strip structure in this embodiment is all identical with embodiment 1.

[0045] In this embodiment, in this embodiment, the ratio of the Plasmodium falciparum histidine-rich protein II monoclonal antibody to the colored latex particles is 1:15, and the non-Plasmodium falciparum lactate dehydrogenase monoclonal antibody The ratio of the colored latex particles to the colored latex particles is 1:15, the concentrations of the colored latex particles-labeled Plasmodium falciparum histidine-rich protein II monoclonal antibody and the non-Plasmodium falciparum lactate dehydrogenase monoclonal antibody are both 10 μg / ml, and coated The dilution parameter on the marker pad is 28cm 2 / ml. The coating concentration of the Plasmodium falciparum histidine-rich protein II monoclonal antibody in the detection zone is 1.7mg / ml; the coating concentration of the non-Plasmodium falciparum lactate dehydrogenase monoclonal antibody in the detection zone The concentrat...

Embodiment 3

[0049] The detection test strip structure in this embodiment is all identical with embodiment 1.

[0050] In this embodiment, in this embodiment, the ratio of the Plasmodium falciparum histidine-rich protein II monoclonal antibody to the colored latex particles is 1:20, and the non-Plasmodium falciparum lactate dehydrogenase monoclonal antibody The ratio of the colored latex particles to the colored latex particles is 1:20, the concentrations of the colored latex particle-labeled Plasmodium falciparum histidine-rich protein II monoclonal antibody and the non-Plasmodium falciparum lactate dehydrogenase monoclonal antibody are both 15 μg / ml, and coated The dilution parameter on the marker pad is 25cm 2 / ml. The coating concentration of the Plasmodium falciparum histidine-rich protein II monoclonal antibody in the detection area and the coating concentration of the non-Plasmodium falciparum lactate dehydrogenase monoclonal antibody in the detection area are both 2.0mg / ml; the ...

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Abstract

The invention discloses an immunochromatographic test strip for rapidly detecting malaria and a preparation method thereof. The test strip is formed by sticking a sample pad, a labeling pad, a coating membrane and absorbent paper to a substrate in sequence through lap joint, wherein colored latex particle labeled plasmodium falciparum histidine-rich protein II monoclonal antibody and non-plasmodium falciparum lactic dehydrogenase monoclonal antibody are coated on the labeling pad; the coating membrane comprises detection regions and a control region; the detection regions are coated by plasmodium falciparum histidine-rich protein II monoclonal antibody and non-plasmodium falciparum lactic dehydrogenase monoclonal antibody with epitopes different from the epitopes of the monoclonal antibodies on the labeling pad; and the control region is coated by an anti-mouse IgG (immunoglobulin G) monoclonal antibody. The test strip improves the accuracy and convenience of malaria screening, is simple and convenient to operate and has the advantages of rapidness, simpleness, convenience and intuition.

Description

technical field [0001] The invention belongs to the field of medical testing. Specifically, the invention relates to an immunochromatographic detection test strip for rapid joint detection of falciparum malaria (P.f) and non-falciparum malaria (P.v, P.o, P.m) and a preparation method thereof. Background technique [0002] Malaria is a vector-borne infectious disease that seriously endangers human health. There are four kinds of Plasmodium parasites in the human body: Plasmodium falciparum (Plasmodiμm falciparμm), Plasmodium vivax (Plasmodiμm vivax), Plasmodium malariae (Plasmodiμm malariae) and Plasmodium ovale. The former two are the most common in our country. Blood testing for Plasmodium is still the most reliable method for diagnosing malaria so far. This method can identify the species and stage of the parasite, but it is time-consuming and laborious, and requires skilled technicians and certain laboratory conditions. The laboratory diagnosis of malaria mainly relies ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/573G01N33/532G01N33/558G01N33/577
CPCY02A50/30
Inventor 王继华李国存
Owner GUANGZHOU WONDFO BIOTECH
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