464 results about "Colloidal au" patented technology

The invention discloses a colloidal gold kit for jointly detecting coronavirus IgM / IgG antibody, and a preparation method thereof, and relates to the field of biological medicine. Whether anti-novel coronavirus nucleocapsid protein IgM antibody and / or anti-novel coronavirus nucleocapsid protein IgG antibody exists in human serum or plasma or not by adopting an antigen-antibody sandwich method anda colloidal gold immunochromatography method principle, the novel coronavirus nucleocapsid protein containing 6xHis mark is marked by applying colloidal gold, thereby forming gold-marked N protein tobe adsorbed on a gold-marked pad, the novel coronavirus nucleocapsid protein containing 6xHis mark is used as an indication marker, the mouse-anti-human u chain monoclonal antibody is coated on the IgM detection line of a NC membrane, the mouse-anti-human IgG monoclonal antibody is coated on the IgG detection line and the mouse-anti 6xHis monoclonal antibody is coated on a quality control line ofthe NC membrane, the qualitative detection of the anti-novel coronavirus nucleocapsid protein IgG antibody is realized, and the colloidal gold kit disclosed by the invention has the advantages of being convenient to use, high in sensitivity and short in detection time.

The present invention relates to a composition for treating skin comprising an acylated short chain bioactive peptide and fulvic acid, and optionally colloidal gold. The invention further relates to a method for topically administering the composition in an amount therapeutically effective to reduce wrinkles by building the dermal fibroblast matrix. The invention further relates to a method of treating wrinkled skin by topically administering the composition to an individual in need of such treatment.

The invention relates to the technical field of biology, and specifically provides a detection kit for a detection kit for antigens of a novel coronavirus (SARS-CoV-2). According to the detection kitprovided by the invention, the antigens of the SARS-CoV-2 are detected by virtue of a colloidal gold double antibody sandwich method, and two monoclonal antibodies and colloidal gold are mixed for labeling, so that a detection result of the antigens of the SARS-CoV-2 is accurate and high in sensitivity, and meanwhile, the detection rate can be remarkably increased. According to the detection kit,a blank in immunological detection of the SARS-CoV-2 is filled up, and field detection can be realized without a detection instrument, so that the time and the labor are saved, and operation is flexible.

The invention discloses a preparation method of a magnetic sandwich nano immunosensor and application of the preparation method. The preparation method is characterized by comprising the following steps: mixing and stirring a collaurum solution and silane ferriferrous oxide into a colorless and transparent solution, and carrying out magnetic separation by externally applying a magnet to obtain FE3O4 / Au colloid nano magnetic beads; loading horse radish peroxidase (HRP) and secondary antibodies of an object to be measured to the FE3O4 / Au colloid nano magnetic beads to obtain a secondary antibody probe Fe3O4 / Au-HRP-Ab2; electrically depositing Au to a glassy carbon electrode, loading primary antibodies of the object to be measured, and then closing non-specific active sites by using protein to obtain an Abl / Au / GCE electrode; and finally, loading antigen to the Abl / Au / GCE electrode, and then obtaining the sandwich nano immunosensor by combining the magnet probe. The magnetic sandwich nano immunosensor can be used for measuring the concentration of melamine, tonyred, clenbuterol or estrogen in food and has the advantages of high detection speed, high accuracy and sensitivity and low cost.

The invention discloses a novel coronavirus detection kit. The kit comprises a bottom plate, and a sample pad, a colloidal gold pad, an NC membrane and a water absorption pad which are fixed on the bottom plate, wherein a detection line and a quality control line are arranged on the NC membrane, an antigen coated on the colloidal gold pad is a mixed antigen protein composed of N protein marked bycolloidal gold and S-RBD protein marked by colloidal gold, and the detection line is a mouse anti-human IgM antibody and mouse anti-human IgG antibody detection line. The detection kit provided by theinvention takes N protein and S-RBD protein as antigens, can improve the sensitivity of COVID-19 preliminary detection, and is suitable for quick screening of large-scale crowds.

A colloidal gold immunity chromatograph to be used for the inspection of aquatic product, agricultural product and livestock product belongs to the technical field of biological medicine. The presentinvention with high sensitiivty and high specificity and be widely used in the departments of inspection and epidemic prevention to overcome the problems such as short of inspecting means and limitation by the conditions existed in the product inspection for the quatic, and livestock agricultural prdoucts.

The invention belongs to a test paper and a preparation method for quickly semi-quantitative testing the residual quantity of 2, 4-D in synthetic water samples, the invention relates to a GICA reaction which relies on a colloidal gold mark to show color, and the invention belongs to the immunochemistry measurement technical field. The theory of the invention is according to the reaction of 2, 4-D and the accurately quantitative gold mark antibody, and then the reactant has an emulous combination with the 2, 4-D-OVA which is cross-band-shaped distributed on the GICA film, and the content of the 2, 4-D of the sample is judged by the number of the stripe on the GICA film. The number is huge, the content of the 2, 4-D of the sample is high, the LOD of the 2, 4-D is 21ug / l, and the test can be finished in 10 minutes. The invention has practical character and remarkable progress, the traditional multi-step fussy detecting of the agricultural chemical is changed, and the method for quickly semi-quantitative testing the residual quantity of 2, 4-D in one-step is realized. The test paper stripe comprises a soleplate, an absorbent filter, a nitrocellulose membrane, a gold mark pad of anti-2, 4-D antibody and a sample adsorption pad, the nitrocellulose membrane in the middle of the soleplate is used for the experiment reacting region of a cellulose nitrate membrane, 4 detection lines and a quality control line are on the cellulose nitrate membrane, a sample area is on the far left, an absorbent filter is on the other end of the soleplate.

This invention discloses one immune glue gold test bar and process method for organophosphorus in agriculture test technique field, wherein, the test bar comprises PVC underlay, adhesive agent layer, sample pad, combination pad, aqua fortis fiber film, weight band, test band, compare band, overlap band, water absorptive pad, limit line and arrow line. The process method comprises the following steps: processing the medicine BSA load protein coupler; processing the medicine special signal clone antigen; processing sheep, rabbit or mouse antigen IgG; processing glue gold; processing single clone antigen gold label.

The invention relates to a kit for quickly and quantificationally detecting serum amyloid A (SAA) by using an immunogold method. The kit includes lyophilized anti-SAA immunogold, a reaction plate, a sample diluent, a lyophilized colloidal gold complex solution, a scrubbing solution and a sealing solution. The preparation method comprises the following steps: (1) firstly preparing a conjugate of the colloidal gold and anti-SAA immunogold, and preparing lyophilized anti-SAA immunogold; and (2) preparing the sample diluent, the lyophilized colloidal gold complex solution, the scrubbing solution and the sealing solution according to the proportion, preparing the reaction plate, and assembling the prepared objects to a finished product to obtain the kit. By using the kit provided by the invention, a doctor can obtain an SAA measurement result on the spot when a person is ill so as to quickly judge the patient's condition, so that more targeted treatment can be effectively taken on the patient, healing time is shortened and medical cost is reduced. The kit provided by the invention has a good application prospect.

The invention relates to the field of nano material development and application, and discloses a method for synthesizing multi-branch colloidal gold nano particles different in grain diameter and high in light absorbing intensity through colloidal gold seed mediation growth. The method comprises the steps that firstly, a conventional trisodium citrate reduction method is adopted, Au3+ in chloroauric acid molecules is reduced into Au0, and ball-shaped colloidal gold seeds are obtained; then, the colloidal gold seeds are added into a growth solution, wherein the growth solution mainly comprises ultrapure water, chloroauric acid, trisodium citrate and other solutions; a magnetic stirring device is used for performing slow stirring, after the solution is evenly mixed, the solution is heated to 50 DEG C, and the rotating speed is increased at the time; and meanwhile a sufficient hydroquinone solution is added at a time, the temperature of the mixed solution is maintained at 50 DEG C, heating is stopped after the reaction is continuously carried out for 10 min, and a multi-branch colloidal gold solution high in light absorbing intensity can be obtained through cooling to the room temperature. The method has the beneficial effects of being easy to operate, simple in procedure, rapid in reaction, good in controllability, high in repeatability and the like.

The invention provides a preparation method of test paper scrip capable of quickly detecting deoxynivalenol test paper in grain and use thereof, comprising (1) DON monoclonal antibody-colloidal gold label; (2) coating of conjugate releasing mat; (3) coating of cellulose nitrate film; (4) production of test paper scrip. The preparation method has features of specificity, high sensitivity; visual detection result; simple operation and high speed, no need of professional operation, obtaining the detection result in 10min; easy large-scale popularization and application, widely application on foodstuff purchasing scene detection, food safety detection, customs quarantine control or the like, with wide market prospect, bigger economic, social benefits.

The invention discloses a colloidal gold immunochromatography test paper for antibodies of pseudorabies viruses of gE, gB and gD and a preparation method. The test paper comprises a sample absorption area, a gold mark probe area, an immobilization antigen and antibody area, a water absorption area and a supporting plate, and the sample absorption area, the gold mark probe area, the immobilization antigen and antibody area and the water absorption area are laid on the supporting plate and are partially overlapped in sequence. The gold mark probe area is covered with gold mark probes which are mouse anti-swine IgG antibodies marked by colloidal gold. The immobilization antigen and antibody area is provided with a detection line T1 covered with pseudorabies virus gE protein, a detection line T2 covered with pseudorabies virus gB protein, a detection line T3 covered with pseudorabies virus gD protein and a control line C covered with goat anti-mouse IgG antibodies. The test paper is fast in detection, high in accuracy, strong in specificity, easy and convenient to carry and operate and capable of being used for differential diagnosis on PRV wild poisonous infection and vaccine immunity and evaluation on the PRV vaccine immunity effect at the same time.

The invention discloses a colloidal gold immunochromatographic test strip for detecting a neutralizing antibody secreted in vivo after injection of a novel coronavirus vaccine and a preparation methodof the colloidal gold immunochromatographic test strip. The test strip comprises a PVC bottom plate, and a sample pad, a colloidal gold pad, a nitrocellulose membrane and water absorption filter paper are sequentially lapped on the PVC bottom plate from left to right; the colloidal gold pad is coated with a recombinant novel coronavirus RBD protein marked by colloidal gold; and the nitrocellulosemembrane is coated with an angiotensin converting enzyme 2 (ACE2) detection line and coated with a mouse anti-chicken IgG antibody as a quality control line. According to the colloidal gold immunochromatography test strip, a competitive method is adopted to detect the neutralizing antibody in a human body, the sensitivity, specificity, repeatability and stability are high, the recovery rate of atarget compound is high, and the detection result is accurate and reliable. The test strip realizes rapid qualitative detection of the neutralizing antibody in a human body after injection of a novelcoronavirus vaccine, has high sensitivity and small intra-batch and inter-batch difference, and provides great convenience for clinical use.

The invention discloses a reverse typing colloidal gold kit for ABO blood groups and a preparation method thereof. The reverse typing colloidal gold kit for the ABO blood groups comprises a reacting plate, erythrocyte membrane antigen gold particles and a basic solution, wherein 3N reaction holes are formed in the reacting plate and are respectively used for placing A erythrocyte membrane antigen gold particles and a basic solution, B erythrocyte membrane antigen gold particles and a basic solution, and O erythrocyte membrane antigen gold particles and a basic solution; the reaction holes are of circular structures, the bottoms of the holes are smooth and U-shaped; and sealing covers are arranged on the reaction holes. The kit also can be a dry kit; and the erythrocyte membrane antigen gold particles and the basic solution are dried on the reaction holes. According to the reverse typing colloidal gold kit for the ABO blood groups and the preparation method, erythrocyte membrane blood group antigen extracts are prepared and then marked with the colloid gold, and thereby the problem that the fresh erythrocyte just can be stored in a short period can be solved. The kit has the shelf life not less than 12 months at 2 to 20 DEG C, and has the advantages of being high in sensitivity, convenient to use, and relatively low in cost, and identifying the results easily.

The invention provides a system for rapidly detecting new coronavirus 2019-nCoV in a blood sample. The system is used for detecting IgM and IgG antibodies of the new coronavirus 2019-nCoV, and comprises a buckle, a colloidal gold immunochromatography test strip and a sample buffer solution. The colloidal gold immunochromatography-free test strip comprises a sample pad, a whole blood separation membrane, a marking pad, a chromatography membrane, a water absorption pad and a bottom plate which are connected in sequence, a colloidal gold-new coronavirus N protein connecting antigen compound and acolloidal gold-quality control molecular compound are fixed on the marking pad at the same time, and a mouse anti-human IgM-mu chain antibody and a mouse anti-human IgG-mu chain antibody are respectively fixed on a detection line. During detection, after the blood sample to be detected is added to the sample pad, the sample buffer solution is added; if the sample buffer solution is not used, butthe sample is directly detected, the new coronavirus IgM and IgG in the sample cannot be detected or false positive easily occurs, so that the existence of the sample buffer solution is crucial for realizing the detection of the sample.

This invention provides one glue gold test bar to test dengue fever virus IgG and IgM antibody, which separately covers IgM antibody and dengue virus antigen and antigen multiple clone antibody on NC film and combines glue gold label dengue virus reestablish antigen and applies immune film analysis technique and tests dengue virus IgG and IgM antibody.

The invention relates to an immunochromatography quantitative detection instrument and a detection method of colloidal gold, latex and the like for quantitative detection of an immunochromatography test strip. A to-be-detected sample is dripped onto a transverse immunochromatography test strip which is marked in advance to coat a corresponding antigen-antibody, then the test strip is inserted into a detection tank of the immunochromatography quantitative detection instrument which is used for confirming the color chromaticity and background chromaticity of C and T line regional positions in a nitrocellulose membrane of the test strip by virtue of multi-wavelength scanning, identification computational analysis is performed on optical density values of a quality control band and a detection band according to a built-in algorithm of the instrument, then the concentration of positive markers contained in the to-be-detected sample is obtained, a report is printed, and a result value is stored. By using the immunochromatography quantitative detection instrument and the detection method, the influence of the difference among different immunochromatography test strips on detection results can be avoided, and precise and stable quantitative detection is really realized. In addition, the immunochromatography quantitative detection instrument and the detection method have the advantages of good open generality and are suitable for determining the detection results of immune test paper with different specifications and different detection types.

The invention relates to a colloidal gold immune chromatographic test paper box of a silver staining enhancement technology. The test paper box is characterized by mainly comprising a colloidal gold immune chromatographic test paper strip, a silver salt-coated bottom membrane and a top membrane which contains a reducing agent for silver salt reduction reaction. The test paper box has the advantages of easiness for operation, judgment and storage, high sensitivity, high stability, quick detection, clear result, no need of any instrument or equipment and the like, is particularly suitable for field test of clinical and sudden events and is suitable for large-scale application of epidemiological survey.

The invention discloses an immune colloidal gold detecting card of carbendazin and a preparation method of the immune colloidal gold detecting card, and relates to the technical field of animal source food veterinary drug residue detection. A test strip in a shell of the detecting card is composed of a PVC glue plate, a sample cushion, a colloidal gold conjugate pad, a coating film and a water absorbing cushion, wherein a colloidal gold film is a glass cellulose membrane containing carbendazin monoclonal antibodies, the coating film is a nitrocellulose film and is provided with a line T and a line C, the line T is wrapped by carbendazin protein conjugates, and the line C is wrapped by goat-anti-mouse IgG antibodies. The detecting card can be effectively used for rapidly detecting carbendazin and is convenient to use, rapid and accurate in result.

This invention discloses a way for fast checking the immunological colloid gold test strip left by the sulfadiazine and it's making method, which belongs to the immunochemistry fast measure technique filed. The test strip of this invention includes: the sample mat, the combining mat, the cellulose nitrate film, the sopping mat and the PVC backing, which characterized in that the sample mat, the combining mat, the cellulose nitrate film and the sopping mat inhibit the PVC backing in order; the combining mat is marked with the anti-sulfadiazine monoclonal antibody-colloid gold marker, and the monoclonal antibody is obtained by the secretion of the hybridomas cell line BDRXN(the conserving number is CCTCC-C200522) The cellulose nitrate film is covered with the checking line that is composed of the sulfadiazine-carrier protein joined matter and the quality control line that is composed of the rabbit-anti-mouse IgG. The test strip of this invention has the following advantages: the sensitivity is high, the special isomerism is strong, the operation is simple and checking is fast and exactly.

The invention discloses a making method of hapten and holoantigen of semicarbazide derivant, which is characterized by the following: establishing immunity method to detect SEM in the food through ELISA method; testing SEM qualitatively and quantitatively; fitting for SEM agent box (ELISA agent box, colloidal gold test paper and so on) of edible animal tissue.

The invention relates to colloidal gold test paper for rapidly detecting an antibody of a porcine reproductive and respiratory syndrome virus (PRRSV). The test paper is characterized in that: the test paper is composed of a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad which are sequentially attached to a nonabsorbent supporting sheet, wherein the combination pad is covered by PRRSVNsp9 proteins marked by colloidal gold; and the nitrocellulose membrane is respectively covered by a detection line T line composed of staphylococcus proteins A (SPA) and a quality control line C line composed of PRRSVNsp9 monoclonal antibodies 2D6. According to the invention, by utilizing an immunological principle that antigens and antibodies can be specially combined, the antigen marked by the colloidal gold is combined with the corresponding antibody and the conjugate of the antigen and the antibody is combined with the SPA to form an antigen-antibody-SPA conjugate, so as to form a color reaction on the nitrocellulose membrane (NC membrane) to be observed by naked eyes. The test paper provided by the invention has the characteristics of simplicity, fastness, sensitivity, good specificity and the like. And the price is low, so that the test paper is applicable to basically or clinically detecting the antibody of the porcine reproductive and respiratory syndrome virus. The test paper has the obvious advantages of strong specificity, high flexibility, simplicity in operation and fastness in diagnosis, and can be used for rapidly diagnosing the porcine reproductive and respiratory syndrome virus.

The invention provides a colloidal gold test paper strip for detecting a respiratory syncytial virus (RSV) antibody. In the colloidal gold test paper strip, the IgA antibody of the respiratory syncytial virus is detected by enveloping an antihuman IgA polyclonal antibody on a nitrocellulose membrane (NC membrane), and combining a respiratory syncytial virus specific recombinant antigen marked by colloidal gold and by using immune chromatography technology. The IgA antibody of the respiratory syncytial virus can be detected simply, conveniently, quickly and accurately by using the test paper strip, and a detection result is clear and easy to distinguish, so the test paper strip is suitable for batch detection and is suitable for grass-root screening and epidemiological survey, plays the role in assisting diagnosis of the early stage and medium stage of the infection of the respiratory syncytial virus and has a reference significance for judging whether children have the immunity of RSV resistance or not.

The invention belongs to the field of clinical laboratory techniques and in particular relates to a colloidal gold test strip for quantitatively detecting human bone metabolic biomarker and a detection device applying same. The test strip provided by the invention comprises a sample pad, a glass fiber mat coated with gold-labeled streptavidin, a glass fiber mat coated with a gold-labeled antibody, a nitrocellulose membrane and an absorbent pad which are sequentially adhered with one another on a bottom plate in a staggered manner. A quality control line pre-enveloped with a goat anti-rat IgG antibody is formed in the nitrocellulose membrane; a detection line parallel to the quality control line is formed in the nitrocellulose membrane; an antibody capable of being specifically bound with a to-be-detected antigen human bone metabolic biomarker is coated on the detection line; the gold-labeled antibody is an antibody capable of being specifically bound with the to-be-detected antigen human bone metabolic biomarker. According to the test strip provided by the invention, the human bone metabolic biomarker can be quantitatively detected, the requirement on instruments is low, the time of clinically detecting the human bone metabolic biomarker is shortened, and the detection complexity and cost can be reduced.

The invention provides a colloidal gold immunoassay instrument, which comprises an equipment bottom plate, a reagent strip rotary loading unit, a sample unit, a sample loading unit, a reaction disc unit and a detection unit, wherein the equipment bottom plate is used for providing an installing space; the reagent strip rotary loading unit is used for automatically loading a reagent strip; the sample unit is arranged on the equipment bottom plate and is used for providing a sample to be detected; the sample loading unit is arranged on the equipment bottom plate and is used for loading the sample to be detected on the sample unit; the reaction disc unit is arranged on the equipment bottom plate and is used for receiving the reagent strip loaded by the reagent strip rotary loading unit and the sample to be detected, loaded by the sample loading unit; the sample to be detected takes a reaction with the reagent strip; the detection unit is used for receiving the reacted reagent strip on thereaction disc unit and performs detection. The colloidal gold immunoassay instrument provided by the invention can realize the simultaneous detection of various samples; the functions are various; the installation of the reagent strip is convenient; the structure is simple.

A method of using immune chromatography semi ¿C quantitative test paper to detect tetracycline sticks sample solution absorption portion, colloidal gold labeled portion, detecting reaction portion and water absorption portion in sequence on backing of test paper . The detecting reaction portion is prepared as coating 1 ¿C 3 bar of tetracycline antibody or 1 ¿C 3 bar of detection antigen as detection line and coating 1 ¿C 3 bar of IgG for anti ¿C second generic animal protein as reference line, and using 2 ¿C 4 bar of combination line formed by said two lines being used no more than one bar at the same time in combination.

The invention discloses a test strip for detecting a novel coronavirus antibody, and a preparation method and application of the test strip. The colloidal gold immunochromatography test strip preparedby the method has good sensitivity, specificity and stability, is convenient to store, long in storage life, simple to operate and high in detection speed, is suitable for rapid diagnosis of SARS-CoV-2 virus infection, and can be used for in-situ detection of basic tissues.

The invention discloses an ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method thereof used for the problem of fast detection for pregnancy, comprising a piece of detecting test paper and urine sample treatment solution; wherein, the detecting test paper consists of a base plate, a cellulose nitrate membrane, a gold label anti-Beta HCG monoclone antibody layer, an Alpha-HCG monoclone antibody line, a sheep anti-rat polyclonal antibody line, an absorbing pad and a sample pad; the matching composition of the urine sample treatment solution is that: 12.5 to 18.0 portions of disodium hydrogen phosphate dodecahydrate, 1.2 to 1.7 portions of sodium dihydrogen phosphate dihydrate, 40.0 to 48.0 portions of sodium chloride and 4600 to 5300 portions of distilled water; the ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method of the invention are characterized by fast and simple detection, the operation of blood-drawing detection for 2 to 6 hours can be replaced by 2 to 3 minutes with time-saving, manual-saving and cost-saving; besides, the ectopic pregnancy and pregnant days colloid gold detection agent and manufacturing method thereof have the advantages of detecting ectopic pregnancy and pregnant days in a semiquantitative way, etc., and can be used for external detection of the pregnant condition and artificial abortion and assisted diagnosis.

The invention is a colloidal gold labeling lap gene monoclonal antibody measuring Listeria monocytogenes kit, belongs to the technical field of the hygiene analysis and medical test, and is characterized by comprising the following steps: a. primer designing; b. reaction conditions for PCR amplification; c. reaction conditions of agarose gel electrophoresis (AGE); e. applying heat shock conversion method; f. preparing plasmid by SDS cracking process clean up kit; g. induction expression of protein; h. SDS-PAGE electrophoresis; j. cutting the required strips from the SDS-PAGE electrophoresis gel; and k. preparing colloidal gold by using a trisodium citrate reduction method with little improvement. The invention has the beneficial effects of improving the limitation that only single legionella single antigen can be measured. The invention also features pure antibody and high valence and can improve accuracy and sensitivity. Compared with ELISA method and PCR method, the measurement for Listeria monocytogenes in the invention is rapid and convenient by colloid gold-immunochromatography assay.

The invention discloses a neomycin immuno-colloidal gold detection card and a preparation method thereof, and relates to the technical field of beta-adrenergic receptor agonist detection. The test strip on the shell of the detection card is composed of a PVC rubber sheet, a sample pad, a colloidal gold combined pad, a coating membrane, and a water absorbing pad; the colloidal gold membrane is a glass cellulose membrane containing a neomycin monoclonal antibody; the coating membrane is a nitrocellulose membrane and is provided with a T line and C line, neomycin protein conjugate is coated in the T line, and goat anti mouse IgG antibody is coated in the C line. The provided detection card is used to detect neomycin in a short time, and has the advantages of convenience, quickness, and precise results.