The invention discloses a
colloidal gold test strip for rapidly detecting double viruses of
tobacco mosaic virus-
potato virus Y (TMV-PVY). The test strip consists of a sample pad, a
colloidal gold pad, an NC film, a piece of water
absorption filter paper and a back lining, wherein the
colloidal gold pad is enveloped with a TMV
specific antibody and a PVY
specific antibody which are marked by colloidal gold, and the TMV
specific antibody and the PVY specific
antibody are respectively generated by
secretion by
hybridoma cell lines, i.e., BALB / c-15-50 and BALB / c-15-8; the NC film is provided with a detection line and a
control line; the detection line is enveloped with specific antigens of the TMV and the PVY; the
control line is enveloped with a second
antibody marked by the colloidal gold. The
rapid detection colloidal gold test strip is especially suitable for the detection on the TMV and the PVY in Guangdong tobacco-growing areas, and is rapid, sensitive and accurate in dection, low in cost and simple and convenient in operation; the test strip can be used to diagnose the two viruses at the same time by one-time sampling, thus being very suitable for field
primary screening of a great batch of samples; therefore, the
colloidal gold test strip has a good application value in actual production and is good in popularization and application prospects.