35results about How to "Qualitative detection is accurate" patented technology

The invention relates to a typing detection kit of a multi-PCR fluorescence labelled probe for FK506 personalized medication related genes. According to three specific SNP sites, with high pertinence to metabolism of FK506, on CYP3A4 and CYP3A5 encoding genes, three pairs of amplification primers and three fluorescent probes are designed, a PCR instrument is adopted to amplify to-be-detected three segments of DNA sequences, hybridization of the fluorescent probes and amplified products is utilized to detect hybridization peaks in a reaction system, and Tm values displayed on the standard hybridization peaks for gene typing are determined as the result judgment standards by construction and detection based on wild-type and mutant standard plasmids. The typing detection kit is high in method flexibility, strong in specificity, simple and convenient to operate, and straightforward and clear in result observation, and two genetypes have significant differences and are easy to type. The typing detection kit is suitable for one-tube triple quick detection of three SNP sites of FK506 metabolism related genes.

The invention discloses a primer probe composition for identifying sources of animals including donkeys, horses and foxes. The primer probe composition comprises a pair of general primers, three special TaqMan probes, a competitive type interior label and a special TaqMan probe of the competitive type interior label. The invention further discloses a kit comprising the primer probe composition and a multiplex real-time fluorescence quantitative PCR detecting method. The primer probes are high in specificity and sensitivity, whether source ingredients of three animals including the donkeys, the horses and the foxes are contained in a sample or not can be detected, false negative can be effectively indicated, the detection accuracy is improved, and a new way is provided for authentication searching of the source ingredients of the multiple animals in skin, meat and processed products of the skin and the meat.

The invention discloses a protein fingerprint model formed through drawing according to mass-to-charge ratios of a plurality of proteins and protein crest strength coefficients, wherein the mass-to-charge ratios are 1097, 1777, 2551, 2988, 3381, 3708, 5492, 6007, 7144 and 8012. The invention further discloses a protein detection and analysis system based on the model and a laser desorption / ionization time of flight mass spectrometry detection method for anthrax. In the detection method, protein spectrums of samples are compared with the model to detect the anthrax in the samples qualitatively. The protein fingerprint model contains specific protein spectrum information of the anthrax, is provided with a specific recognition function, and can be applied to commercialized detection and analysis systems of the anthrax. According to the protein fingerprint model, the system and an application thereof for the anthrax, the detection method which is high in sensitivity, specificity and detection rate is provided for the anthrax and is particularly suitable for port check and inspection departments.

The invention discloses a protein fingerprint model for Burkholderia gladioli and application thereof. The model is drafted according to the mass-to-charge ratios of the protein of Burkholderia gladioli and the peak intensity coefficients of the protein, wherein the mass-to-charge ratios include 3568.4, 3864.3, 4802.0, 5233.8, 6829.7, 7133.9, 7422.1, 7701.7, 9602.7 and 10153.8. A detection method comprises the following steps: preparation of a sample protein chip; detection with matrix-assisted laser desorption ionization time-of-flight mass spectrometer; and comparison of the protein fingerprint of the sample. The protein fingerprint model can qualitatively detect whether a sample contains Burkholderia gladioli or not, can be used for detection of commercial Burkholderia gladioli and is especially applicable to departments like a port inspection and quarantine part with detection requirements for rapidness, high flux and the like.

The invention provides a method for detecting a leopard bone component in a mixture and a primer applied. The method mainly comprises steps of: extracting a total DNA from a mixture to be measured; carrying out PCR amplification by a specific primer, the extracted total DNA serving as a template, wherein the specific primer is one pair, two pairs or three pairs of primer selected from the group of SEQ ID Nos.1 and 2, SEQ ID Nos.3 and 4 and SEQ ID Nos.5 and 6; and carrying out analysis and determination on an amplification result. The invention further provides a kit containing the above primer for detecting leopard bone component in the mixture and especially for detecting whether a mixture is leopard bone medicinal material or detecting leopard bone component in a traditional Chinese medicine preparation using a drug of leopard bone or a substitute. The primer of the present invention has high singularity and sensitivity.

The invention provides a method for detecting capra hircus horn ingredients in a mixture and primers used in the same. The method mainly comprises the steps of extracting total DNA from the mixture to be detected; with the extracted total DNA as a template, utilizing specific primers to carry out PCR (polymerase chain reaction) amplification; and analyzing and judging the amplification results, wherein the specific primers are selected from one pair, two pairs or three pairs of such primers as SEQ ID Nos.1 and 2, SEQ ID Nos.3 and 4 and SEQ ID Nos.5 and 6. The invention further provides a kit which comprises the primers and is used for detecting capra hircus horn ingredients in the mixture, especially the crude medicines needing differentiating to judge whether the crude medicines are capra hircus horns or the traditional Chinese medicine preparations using capra hircus horns or substitutes. The primers have higher specificity and sensitivity.

The present invention provides a method for rapidly and sensitively detecting a reducing sulfide. According to the present invention, an ion mobility spectrometry of a photoelectric emission ionization source is adopted as a base detection technology, purified air is adopted as carrier gas and drift gas, and oxidizing reagent ions formed through a photochemical reaction of air under ultraviolet light irradiation are adopted to determine the reducing sulfide; and the method adopts the oxidation reaction to oxidize the reducing sulfide so as to achieve the characteristic detection of the reducing sulfide, and perform quantitative analysis with characteristics of high sensitivity and wide linear range.

The invention relates to the technical field of bioengineering and discloses a VEGF (vascular endothelial growth factor) monoclonal antibody and a fracture healing evaluation antibody chip with the same. The amino acid sequence of a heavy chain variable region of the monoclonal antibody is shown as SEQ ID NO.1, and the amino acid sequence of a light chain variable region of the monoclonal antibody is shown as SEQ ID NO.2. Homogeneity and biological activity unicity of the monoclonal antibody enables antigen antibody reaction results to be convenient for quality control and beneficial to standardization and normalization. The chip with the monoclonal antibody can be directly applied to clinical therapeutic evaluation on fracture healing, solves the technical problems of time and labor consumption, poor result repeatability and the like of the prior art, has extremely high application value in the field of clinical treatment and has wide application prospect.

The invention discloses a colloidal gold test strip for rapidly detecting double viruses of tobacco mosaic virus-potato virus Y (TMV-PVY). The test strip consists of a sample pad, a colloidal gold pad, an NC film, a piece of water absorption filter paper and a back lining, wherein the colloidal gold pad is enveloped with a TMV specific antibody and a PVY specific antibody which are marked by colloidal gold, and the TMV specific antibody and the PVY specific antibody are respectively generated by secretion by hybridoma cell lines, i.e., BALB / c-15-50 and BALB / c-15-8; the NC film is provided with a detection line and a control line; the detection line is enveloped with specific antigens of the TMV and the PVY; the control line is enveloped with a second antibody marked by the colloidal gold. The rapid detection colloidal gold test strip is especially suitable for the detection on the TMV and the PVY in Guangdong tobacco-growing areas, and is rapid, sensitive and accurate in dection, low in cost and simple and convenient in operation; the test strip can be used to diagnose the two viruses at the same time by one-time sampling, thus being very suitable for field primary screening of a great batch of samples; therefore, the colloidal gold test strip has a good application value in actual production and is good in popularization and application prospects.

The invention provides a method for detecting saiga tatarica horn ingredients in a mixture and primers used in the same. The method mainly comprises the steps of extracting total DNA from the mixture to be detected; with the extracted total DNA as a template, utilizing specific primers to carry out PCR (polymerase chain reaction) amplification; and analyzing and judging the amplification results,wherein the specific primers are selected from one pair, two pairs or three pairs of such primers as SEQ ID Nos.1 and 2, SEQ ID Nos.3 and 4 and SEQ ID Nos.5 and 6. The invention further provides a kit which comprises the primers and is used for detecting saiga tatarica horn ingredients in the mixture, especially the crude medicines needing differentiating to judge whether the crude medicines are saiga tatarica horns or the traditional Chinese medicine preparations using saiga tatarica horns or substitutes. The primers have higher specificity and sensitivity.

The invention provides a method for detecting Osteon Myospalacem Baileyi in a mixture and primers used thereby. The method mainly comprises the following steps: 1, extracting total DNA from a mixture to be detected; 2, carrying out PCR amplification through specific primers with the extracted total DNA as a template, wherein the specific primers are selected from one, two or three of SEQ ID Nos.1and SEQ ID Nos.2, SEQ ID Nos.3 and SEQ ID Nos.4, and SEQ ID Nos.5 and SEQ ID Nos.6; and 3, analyzing and determining amplification results. The invention further provides a kit which contains the primers and is used for discriminating whether a mixture to be detected is an Osteon Myospalacem Baileyi medicine or detecting Osteon Myospalacem Baileyi in a traditional Chinese medicinal preparation containing Osteon Myospalacem Baileyi or a substitute thereof. The primers of the invention have high specificity and sensitivity.

The invention discloses an RPA primer and a detection method for detecting a banana streak virus GF isolate, and relates to the field of biotechnology. Aiming at a conserved sequence of a coat proteingene in the banana streak virus GF isolate, the invention designs the specific RPA primer, a probe and a kit, and accurate qualitative detection can be performed on the banana streak virus GF isolate. Based on the RPA amplification primer and the probe, the kit has high accuracy and detection sensitivity of 0.1ng, and is simple and rapid to operate, provides technical support for gene specificitydetection in the banana streak virus GF isolate, has scientific guiding significance for banana virus disease prevention and control and product inspection and quarantine, and also has important significance for reducing blind use of pesticides, reducing the production cost and reducing pesticide environmental pollution.

The invention discloses a tobacco mosaic virus (TMV) single colloidal gold quick detection test paper strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a back lining. The colloidal gold pad is coated by a TMV specific antibody marked by colloidal gold. The antibody is secreted by hybridoma cell strain BALB / c-15-50; a detection line and a control line are arranged on the NC membrane, wherein the detection line is coated with specific antigens of three viruses, and the control line is coated with a secondary antibody marked by the colloidal gold. The test paper strip is mainly used for detection on the TMV in tobacco planting regions in Guangdong, is quick, sensitive, accurate and low-cost, has simple operations and achieves simultaneous diagnosis of multiple viruses through one-time sample collection, and is very suitable for in-site primary screening of a large amount of samples. The test paper strip has great application value in practical production and has good promotion and application prospects.

The invention discloses a method for realizing visual nucleic acid testing by composite magnetic beads and relates to the technical field of nucleic acid testing. The method comprises steps as follows: proteinase K is added to a to-be-tested sample, a lysate and extracting magnetic beads are added and mixed uniformly for a reaction, then the magnetic beads are adsorbed in a magnetic environment, acleaning solution is added, uniform mixing is carried out, the magnetic beads are re-suspended and adsorbed magnetically, liquid is removed, and after drying is performed, the magnetic beads are re-suspended by an eluent; an isothermal amplification reaction solution and a biotin modified primer are added, isothermal amplification is carried out, and an amplification product is obtained; amplicon-targetedly-enriching magnetic beads modified with avidin and FAM-DNA-NH2-fluorescence probe magnetic beads are added, amplicon magnetic beads are formed after incubation, the magnetic beads are magnetically adsorbed, a supernatant is removed, and mixed magnetic beads are obtained by drying; and a Cas12a+crRNA premixed solution is added, re-suspending is carried out, and after a reaction, the magnetic beads are magnetically adsorbed, and observation is performed under blue light. According to the method, nucleic acid extraction, amplification and detection are completed in a tube, reaction solutions of different conditions are replaced in the processes, free switching of different reaction systems can be realized by extraction samples, primers and probes combined on the magnetic beads, andtherefore, the accurate qualitative detection is completed.

The invention relates to a method for detecting in-vivo metabolites of protopanaxadiol in blood plasma or urine samples, belonging to the field of pharmacoanalysis. According to the method, an ultra-high performance liquid chromatograph Agilent 1290 and a high-resolution mass spectrometer AB 5600+Q TOF are cooperatively used; an electrospray ionization (ESI) source is employed; a chromatographic column used in the invention is Agilent Eclipse plus C18RRHD with a filler particle size of 1.8 [mu]m, an inner diameter of 2.1 mm and a length of 50 mm; a mobile phase consisting of a 0.5% aqueous formic acid solution (A) and acetonitrile (B) is employed for gradient elution; flow velocity is 0.3 mL / min; column temperature is 30 DEG C; the elution procedures of gradient elution with the mobile phase comprise elution with 95% A in a time period of 0 to 1 min, elution with 95-75% A in a time period of 1 to 1.5 min, elution with 75-25% A in a time period of 1.5 to 6.0 min, elution with 25-0% A in a time period of 6.0 to 12.0 min, elution with 0% A in a time period of 12.0 to 13.0 min and elution with 0-95% A in a time period of 13.0 to 15.0 min; and qualitative detection is carried out the metabolites of protopanaxadiol inside the human body, and unknown metabolites are identified through comparison with blank samples. The method provided by the invention has the advantages of high speed, sensitivity, high resolution, high separation degree, etc.

The invention discloses a terahertz spectrum qualitative detection method for a student school uniform textile material, which comprises the following steps: preparing different textile material experiment samples of a student school uniform, obtaining terahertz absorbance spectrums of different textile materials by using a terahertz time-domain spectroscopy system, and extracting feature information of a grey-scale map of the terahertz absorbance spectrums by using a Krawtchouk moment; combining an improved particle swarm algorithm with a support vector machine to establish a qualitative model, obtaining a classification identification model of different textile materials; collecting the textile materials on the school uniform, obtaining the feature information of the textile materials on the school uniform, and sending the feature information of the textile materials on the school uniform into the classification identification model of the corresponding textile materials for qualitative detection. The terahertz spectrum can be used for carrying out sexual detection on the textile material of the school uniform, so that the school uniform quality of primary and secondary school students is ensured, and primary and secondary school student safety incidents caused by the school uniform quality are avoided.

The invention discloses a PVY single virus colloidal gold rapid test strip which is composed of a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a backing; the colloidal gold pad is coated with a colloidal gold-labeled specific PVY antibody, and the antibody is secreted from a hybridoma cell strain BALBc-15-8; the NC membrane is provided with a test line and a control line, the test line is coated with a specific PVY antigen, and the control line is coated with a colloidal gold-labeled secondary antibody. The test strip is rapid to operate, sensitive, accurate, low in cost and easy and convenient to operate for testing PVY in Guangdong tobacco-growing areas, achieves simultaneous multi-sample diagnosis in one-time sampling, is very suitable for conducting site primary screening on a large scale of samples and has an application value in practical production and a good application and popularization prospect.

The invention belongs to the technical field of material analysis, and provides a method for detecting amantadine compounds and triazine herbicides in algae. According to the method, acetonitrile, ethyl acetate, a formic acid-acetonitrile mixed solution with the formic acid volume concentration of 1% or a formic acid-ethyl acetate mixed solution with the formic acid volume concentration of 1% are adopted as an extracting agent, so that amantadine compounds and triazine herbicides in an algae sample can be released; a PSA adsorbent is used as an adsorbent; impurities which have great interference on amantadine compounds and triazine herbicides in an extracting solution can be adsorbed and removed to the greatest extent, qualitative detection is performed by adopting ultrahigh performance liquid chromatography, primary mass spectrometry and secondary mass spectrometry, the qualitative accuracy can be improved, false positive can be eliminated, and the detection accuracy can be improved; the amantadine compounds and the triazine herbicides can be accurately quantified by performing quantitative analysis on the amantadine compounds and the triazine herbicides by using ultrahigh liquid chromatography and primary chromatography, and the method has relatively high detection sensitivity.

The invention provides a method for rapidly detecting the drug resistance of klebsiella pneumoniaeto imipenem through real-time fluorescent PCR, which comprises the following steps: scraping a klebsiella pneumoniae strain to be detected, dissolving the klebsiella pneumoniae strain in water, carrying out thermal cracking, centrifuging, and taking supernatant as a PCR detection sample; respectively using five groups of specific primers and probes to detect whether a sample contains carbapenemase gene blaKPC, blaNDM, blaIMP, blaOXA-48 and ompK36 mutation sequences or not through real-time fluorescent PCR, and judging the drug resistance of a sample strain to imipenem; The method has the beneficial effects that the target spot is new, and the drug resistance of the klebsiella pneumoniae carrying blaKPC to imipenem is detected and judged by virtue of the ompK36 mutation sequence; the speed is high, the drug resistance of the strain can be determined only by culturing for 1 day after pure bacteria are obtained by an existing clinical common method, however, the method only needs 2 hours, and the detection time is greatly shortened; and the result is precise and the flux is high.

The invention discloses a recombinase polymerase amplification (RPA) primer, probe and kit for detecting TiLV of tilapia, and relates to the technical field of biology. The technical scheme is characterized in that the specific RPA primer, probe and kit are designed for the ORF10 gene of tilapia lake virus (TiLV) of tilapia, so that accurate qualitative detection can be performed on the tilapia lake virus of tilapia. Based on the RPA amplification primer, the probe and the kit, accuracy is high, operation is simple and rapid, and scientific guiding significance is achieved on culture and import and export inspection and quarantine of tilapia.

The invention relates to a quality control method of a medicine capable of clearing lung and eliminating phlegm to relieve cough. Based on the foundation of original standards of Qingjinzhisouhuatanwan, a thin layer chromatography method of radix peucedani is established and a method, which has the advantages of good repeatability, high specificity, clear spot coloration and easily-judged result, is screened and brought into a quality standard revised in the standards, so that the quality control of the medicine is improved, the qualitative determination of the medicine is relatively accurate and the qulity is relatively easy to control.

The invention relates to a quality control method of a medicine for clearing lung, eliminating phlegm and relieving coughs. On the basis of an original standard of Qingjin cough-relieving and phlegm-eliminating pills, a thin-layer chromatography method of bitter oranges and pummel peels is established, a method with good reproducibility, high specificity, clear spot color development and easy-to-judge results is screened out and incorporated into an amended quality standard, the quality control on the medicine is improved, so that qualitative detection of the medicine is more accurate, and the quality is easier to control.

The invention discloses a method and a kit for detecting exogenous N-Methylpyrrole-2-carboxaldehyde, wherein the method for detecting the exogenous N-Methylpyrrole-2-carboxaldehyde comprises the following steps: extracting a sample to obtain an extracting solution; separating the extracting solution by gas chromatography to obtain separated N-Methylpyrrole-2-carboxaldehyde; detecting an isotope ratio of the separated N-Methylpyrrole-2-carboxaldehyde so as to obtain a detection result of the isotope ratio; and determining whether the sample contains exogenous N-Methylpyrrole-2-carboxaldehyde based on the detection result of the isotope ratio. The method can judge whether a sample contains exogenous N-Methylpyrrole-2-carboxaldehyde.

The invention relates to the field of detection, in particular to the field of detection of manuka honey, and more particularly relates to a method for quickly determining the nature of manuka honey, which is a molecular fluorescence method and comprises the following steps of: irradiating a sample to be detected by using 270nm as an excitation wavelength, scanning an emission spectrum in a range of 280-550nm, and judging by using fluorescence peaks at 305nm and 360nm and the intensity of the fluorescence peaks. The qualitative detection method disclosed by the invention can be used for rapidly, accurately and qualitatively detecting single-flower manuka honey, multi-flower manuka honey and other adulterated honey. According to the method, expensive instruments such as a high performance liquid chromatograph and the like and complicated separation treatment are not needed, so that the manual honey does not need to be sent to a specific laboratory for detection, and various occasions for qualitative detection of the manual honey in actual work can be met.