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Protein fingerprint model for Burkholderia gladioli and application thereof

A technology of Burkholderia Pope, Burkholderia Pope

Inactive Publication Date: 2016-05-04
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no protein fingerprint model of Burkholderia gladiolus for detection or identification, nor is there a detection method based on this protein fingerprint model

Method used

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  • Protein fingerprint model for Burkholderia gladioli and application thereof
  • Protein fingerprint model for Burkholderia gladioli and application thereof
  • Protein fingerprint model for Burkholderia gladioli and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0038] 1. Materials

[0039] 1. Tested strains

[0040] Table 1 All tested strain numbers and sources

[0041] Strain number

strain name

1.3050

Burkholderia gladioli

1.2741

Burkholderia caryophylli

1.2892

Burkholderia gladioli

1.3059

Burkholderia stabilis

1.3077

Burkholderia cenocepacia

1.3133

Burkholderia cepacia

4 --> 1.3167

Comamonas testosteronii

1.3360

Burkholderia gladioli

1.3809

Burkholderia pyrrocinia

1.3824

Burkholderia gladioli

1.3898

Burkholderia multivorans

1.3973

Ralstonia pickettii

1.10195

Burkholderia tropica

1.10200

Burkholderia tropica

[0042] 2. Experimental reagents

[0043] (1) Solute of matrix solution

[0044] α-cyano-4-hydroxycinnamic acid (HCCA; Alpha-cyano-4hydroxy-cinnamicacid), from BrukerDaltonics, Germany

[0045] (2) Other reagents

[0046] Ethanol (Ethanolabsolute, HPLC / s...

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Abstract

The invention discloses a protein fingerprint model for Burkholderia gladioli and application thereof. The model is drafted according to the mass-to-charge ratios of the protein of Burkholderia gladioli and the peak intensity coefficients of the protein, wherein the mass-to-charge ratios include 3568.4, 3864.3, 4802.0, 5233.8, 6829.7, 7133.9, 7422.1, 7701.7, 9602.7 and 10153.8. A detection method comprises the following steps: preparation of a sample protein chip; detection with matrix-assisted laser desorption ionization time-of-flight mass spectrometer; and comparison of the protein fingerprint of the sample. The protein fingerprint model can qualitatively detect whether a sample contains Burkholderia gladioli or not, can be used for detection of commercial Burkholderia gladioli and is especially applicable to departments like a port inspection and quarantine part with detection requirements for rapidness, high flux and the like.

Description

technical field [0001] The invention relates to a protein fingerprint model of Burkholderia gladiolus and the application of the model in detection and identification of Burkholderia gladiolus. It belongs to the field of microbiological detection. Background technique [0002] Burkholderia gladioli is a plant pathogenic bacterium, an opportunistic pathogenic bacterium in humans (which can cause pneumonia symptoms in humans), and is also a key poisoning and pathogenic bacterium in food. Food poisoning caused by contamination of coconut poisonous fermented food in Indonesia, fermented rice noodles in Northeast China and fresh white fungus has attracted widespread attention. The fatality rate of food poisoning caused by it is extremely high. In the food poisoning incidents of fermented rice noodles in northeast and southwest my country, the mortality rate reached more than 40%. Burkholderia gladiolus was first mentioned in 1921 as a phytopathogenic bacterium capable of infect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N33/6851
Inventor 吴兴海冯建军吴振兴孙敏肖西志封立平段维军邓丛良房保海李健勇冯黎霞
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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