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476 results about "Tilapia sp." patented technology

Frozen tilapia mossambica slice and its processing method

The present invention discloses a kind of frozen tilapia mossambica fish fillets containing freezing protective agent trehalose and its processing method. Said processing method includes the following steps: making the tilapia mossambica fish undergo the processes of cleaning dressing, boning, skinning and filleting treatments, then adding protective agent to treat fish fillets, taking out said fish fillets, draining to remove excess solution and freezing below-18 deg.C so as to obtain the invented frozen tilapia mossambica fish fillets. The described protective agent is an aqueous solution containing trehalose, glycine and sodium chloride.
Owner:南宁中诺生物工程有限责任公司 +2

Method for preparing ACE inhibition peptide originate from fish skin

A method for preparing ACE inhibition peptides from fish skin is provided. The method is prepared with tilapia skin, extracts fish skin albumen from tilapia skin, adapts prolease to hydrolysis through enzyme extinguishing, debitterizing, decolouring, de-flavoring, filtering, ion exchanging and concentrating to obtain fish skin proteolysis liquor, after untruly filter (cutoff molecular mass is 9000Da) the TSP-I, collect high activity component by SephadexG-15 gel chromatography, freeze and dry to obtain high activity ACE inhibition peptides. The invention has important meaning for satisfying integrated utilization of tilapia skin wastes and improving its attachment.
Owner:NANCHANG UNIV

Collagen protein and collagen polypeptides, preparation thereof and applications

The invention provides a collagen and a collagen polypeptide, wherein, the weight percentage of hydroxylysine is more than 1.3 percent. The invention also provides the preparation technology and the application of the collagen and the collagen polypeptide. The invention can make full use of inexpensive raw materials including the fish skin and fish scale of tilapias to produce products with high added value. The collagen polypeptide is used for treating arthritis with a definite drug effect, and the clinical dose is considerably less than other collagen polypeptides. The collagen polypeptide can solve the pollution problem of fish skin wastes, and at the same time avoid the risk of infectious diseases which can be caused by the collagen of land animals. The collagen and the collagen polypeptide have the advantages of considerable economic benefits, energy conservation and environmental protection, simple process equipment, low cost and easy industrialization.
Owner:史宗洁

Fermented low-salt semi-dried tilapia mossambica, processing method thereof and application thereof

InactiveCN101744316AAchieving Controlled FermentationPromote dissolutionFood preparationSaline waterFlavor
The invention discloses a fermented low-salt semi-dried tilapia mossambica, a processing method thereof and application thereof. The processing method comprises the steps of: removing the internal organs and the scales of the tilapia mossambica; cutting along backside or abdomen; overturning; flatly pressing to form a symmetrical tilapia mossambica piece except the tail of the tilapia mossambica;washing with cleaning water; washing with salt water with 1%-4% of concentration to remove fishy smell and obtain the tilapia mossambica piece without the fishy smell; paving salt on the surface of the tilapia mossambica piece without the fishy smell, wherein the weight of the salt is equal to 3-8% of that of the tilapia mossambica; sousing for 8-24 hours; drying; or soaking for 8-24 hours with 5-10% of salt water to obtain the low-salt tilapia mossambica piece; spraying zymophyte on the surface of the tilapia mossambica piece, wherein the inoculum size of the zymophyte is 105-107 CFU / g fish meat; fermenting for 10-48 hours in a sealing way under the temperature of 12-20 DEG C; and drying the fermented tilapia mossambica piece to obtain the fermented low-salt semi-dried tilapia mossambicawith 30-60% of water content. The product has lubricating meat quality and typical flavor of dried meat. The processing method can be adapted to processing freshwater fishes.
Owner:汕尾市利群农业食品有限公司

Compound feed for preventing fatty liver of tilapia and preparation method of feed

The invention discloses a compound feed for preventing fatty liver of tilapia and a preparation method of the feed. The compound feed is prepared from the following raw materials according to a certain proportion: fish meal, fermented soybean meal, meat and bone meal, rapeseed meal, cottonseed meal, wheat bran, wheat flour, calcium dihydrogen phosphate, multi-vitamin premix, multi-mineral premix, soybean oil, soybean lecithin, chitosan oligosaccharide, astragalus polysaccharide and choline chloride. The preparation comprises the following steps of: respectively crushing and screening all the raw materials (except the soybean oil) by a crushing machine, weighing the flour, the calcium dihydrogen phosphate, the multi-vitamin premix, the multi-mineral premix, the soybean lecithin, the chitosan oligosaccharide, the astragalus polysaccharide, the choline chloride and other raw materials according to the mass fraction in the formula and mixing uniformly, further weighing the remaining raw materials, uniformly stirring and mixing, fully uniformly mixing with water, performing extrusion and pelletization by a feed machine, and drying at the temperature of 55-60 DEG C to prepare the feed for the tilapia. The compound feed has balanced nutrition, is suitable for requirements of healthy growth of the tilapia and can further reduce the accumulation of fat in livers of the tilapia and improve disease resistance of the tilapia.
Owner:YANGTZE RIVER FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

A high-sugar and low-protein tilapia feed

The invention discloses a high-sugar and low-protein tilapia feed. Based on the total mass fraction of 100%, it consists of the following components: fish meal: 0-3%, soybean meal: 16-20%, rapeseed meal: 18-21%, cotton meal: 12-16%, secondary meal: 39- 42%, soybean oil: 2-3%, choline chloride: 0.2-0.5%, sodium chloride: 0.1-0.5%, calcium dihydrogen phosphate: 1-2%, vitamin B1: 0.004-0.006%, vitamin B6: 0.002-0.004%, zinc sulfate: 0.02-0.04%, chromium chloride: 0.0001-0.0003%, vitamin premix: 0.1-0.2% and mineral premix: 0.5-1%. The invention maximizes the amount of secondary powder in the feed, adds several plant protein sources to overcome the problem of amino acid imbalance of a single protein source, and at the same time significantly reduces the amount of fish meal. By optimizing and adjusting the feed formula, supplementing suitable vitamin and mineral premixes to adjust the sugar utilization ability of tilapia, so that the tilapia can make good use of the sugar compound of the present invention, avoiding the damage of fish liver caused by high sugar, Fatty liver is formed, and even the fish body becomes obese, and the symptoms of "big belly" appear.
Owner:广东省农业科学院畜牧研究所 +1

Mixed feed for red tilapias

The invention discloses a mixed feed for red tilapias. The mixed feed is made from the following materials in parts by weight: 8-28 parts of fish powder, 17-28 parts of bean pulp, 10-19 parts of rapeseed meal, 2-3 parts of peanut gluten, 7-8 parts of corn gluten meal, 4-6 parts of dried blood, 20-22 parts of flour, 12-14 parts of DDGS fodder, 3-4 parts of fish oil, 0.1-1 part of lecithin, 2-3 parts of soya-bean oil, 1-2 parts of premix for commercial fish, 0.1-1 part of choline, 1-2 parts of calcium hydrophosphate, 0.5-2 parts of phagostimulant, 0.3-1 part of compound traditional Chinese medicine additive and 0.01-0.05 part of compound probiotics. The mixed feed for red tilapias is balanced in nutrition and essential amino acids, has the effect on resisting bacteria and preventing diseases and boosting immunity, is good in liver and bile protection and palatability, does not need to add antibiotics, and is free of polluting water, green and environment-friendly, and high in cost performance.
Owner:张嘉强

Special formula feed for improving tilapia mossambica meat quality and preparation method thereof

The invention relates to the field of aquatic feed, in particular to special formula feed for improving tilapia mossambica meat quality and a preparation method thereof. Ingredients in a feed formula comprise, by weight, 40-70 parts of broad bean, 2-5 parts of fish meal, 7-12 parts of bran, 9-19 parts of rapeseed dregs, 8-19 parts of peanut meal, 1-2 parts of fish oil, 0.3-0.5 part of glycine betaine, 0.4-0.6 part of choline chloride, 0.6-0.8 part of monocalcium phosphate, 0.10-0.13 part of methionine, 0.06-0.1 part of enzyme preparation, 0.02-0.03 part of allicin, 0.4-0.6 part of multivitamins and 1.0-1.2 parts of complex mineral. The preparation method of the feed is further provided. The feed can improve the tilapia mossambica meat quality to be firm, hard, crisp and tasty, improves growth performance of tilapia mossambica, reduces pollution on water quality and production cost and remarkably improves economical and social benefit.
Owner:福建省淡水水产研究所

Formulation and preparing method of float expanded feed for tilapia

Disclosed is a puffed feed for Tilapia and process for preparation, wherein the constituents include (by weight percent) fish flour 8-12%, soybean 20-30%, meat and bones meal 0-10%, rapeseed waste 20-30%, malt 5-10%, liquid phosphatide 0.5-1%, short flour 3-5%, maize 15-30%, calcium hydrogen phosphate 0.5-1%, composite premixed material 1-2%, soybean oil 0-0.5%, fish oil 0.5-1%.
Owner:GUANGDONG EVERGREEN FEED INDAL

Method and device for filleting killed and headless fish, the abdominal cavity of which is opened up

InactiveUS7090574B2Production of clean filletsMinimal useFish filletingAbdominal cavityTilapia
Certain farmed and / or breeding fish such as tilapia are filleted after the fish have been beheaded, slaughtered and had their ventral cavity opened. Cuts are made in the dorsal region and on the ventral side in the caudal region for exposing the dorsal spokes and ventral spokes, respectively. Then, separating cuts are made over the vertebral column connecting the dorsal and ventral cuts. Before peeling separation in the region of the ventral cavity, the ventral lobes are trimmed by separation of the ends of their ventral lobe portions projecting beyond the ribs. Peeling separation is completed by peeling cuts adapted to the rib contour and made with at least two degrees of freedom.
Owner:NORDISCHER MASCHINENBAU RUD BAADER GMBH CO KG

Fine and smooth tilapia mossambica bone and fish sauce and preparation method thereof

The invention discloses a fine and smooth tilapia mossambica bone and fish sauce and a preparation method thereof. The preparation method comprises: taking tilapia mossambica processing leftovers fish fillet as raw material, and cleaning and mincing for standby; adding acetum into the minced raw material, and carrying out high-pressure cooking; after cooking and softening process, separating cooking liquid, and smashing solid bone and fish blocks by a meat grinder; then, mixing with the cooking liquid; adding clean water accounting for 50-100% of total weight of cooking liquid to adjust the denseness of size; then carrying out rubbing processing on the colloid; ensuring that colloid cycling time is 3-7 minutes; after degassing processing, processing material by a high-pressure microfluidics nanometer homogenizer at the pressure of 60-120MPa to obtain fine and smooth tilapia mossambica bone and fish pulp; and condensing and seasoning the fine and smooth tilapia mossambica bone and fishpulp to obtain the tilapia mossambica bone and fish sauce of different flavours. The invention can fully utilize the nutrition constituent of fish fillet raw material, has high processing speed and small average particle diameter of products and can meet the requirements of fine and smooth taste.
Owner:SOUTH CHINA UNIV OF TECH

Feed additive specially used for tilapia

The invention relates to a feed additive specially used for tilapia. The feed additive is prepared by grinding and mixing the following raw materials in parts by weight: 5-8 parts of rhizoma anemarrhenae, 5-8 parts of catalpa leaves, 5-8 parts of the fruit of Chinese wolfberry, 4-7 parts of rheum officinale, 3-7 parts of cortex magnoliae officinalis, 3-6 parts of agastache rugosus, 3-5 parts of the root of red-rooted salvia, 2-4 parts of radix astragali, 1-2 parts of platycladi seeds, 1-2 parts of acalypha australis, 1-2 parts of bighead atractylodes rhizome and 10-25 parts of a carrier. The feed additive specially used for the tilapia obviously improves the economic benefit of tilapia aquaculture, remarkably improves the anti-stress capability of the tilapia, improves the immunocompetence of the tilapia and reduces the occurrence of nutritional fatty liver disease and streptococcicosis in an aquaculture process, so that the survival rate of the tilapia is increased by 15-22%; and furthermore, the feed additive can promote the growth of the tilapia, and improves the quality of the tilapia meat.
Owner:广西百跃农牧发展有限公司

Culture method of Tilapia

The invention discloses a culture method of Tilapia. The culture method comprises the following steps of: selecting a pond which is 2 meters deep, and disinfecting the pond with unslaked lime after emptying the pond in late October every year; before late November, putting grass carp fries which are all heavier than 250g and feeding with a floating grass carp granulated feed when the water temperature is higher than 15 DEG C; in the next year, putting overwintering Tilapia fries which are all heavier than 200g when the water temperature of the pond is higher than 18 DEG C steadily, and feeding the Tilapia fries twice every day; in late July, fishing 90% of Tilapias for sale, then putting current Tilapia fries which are all heavier than 200g, feeding with a Tilapia floating feed containing 28% of protein in a feed ratio of 1-3%; and fishing all the fishes for sale in late October. By utilizing the culture method, the survival rates of Tilapia and grass carp are higher than 99% and 98% respectively, the yearly output of the pond is improved by 15%, and the commercial fishes are large and uniform in specification and are good in quality. The culture method is a healthy ecological culture mode mainly for culturing Tilapia.
Owner:LIUZHOU YUJIALE FEED

Enclosed aquacultural systems for production of purified recombinant proteins

InactiveUS20050241011A1Optimal production of proteinEasy to produceVirusesClimate change adaptationBiotechnologyTrout
The invention provides a production method for purified recombinant proteins of commercial value prepared in enclosed aquatic systems. The production system provides biosecure containment of genetically engineered aquatic organisms for an alternative production method for complex proteins requiring post-translational modification that is best done in eukaryotic expression systems. Possible aquatic organisms that can be utilized by this invention are crustaceans (e.g. penaeid shrimp or Artemia) and teleost fish (e.g. trout or tilapia).
Owner:ADVANCED BIONUTRITION CORP

Complex of marine oligosaccharides and collagen peptides, preparation method thereof and application thereof

The invention provides an ultraviolet radiation damage resistant complex of marine oligosaccharides and collagen peptides, which is prepared from marine oligosaccharides with antioxidant activity and tilapia mossambica fishskin collagen peptides with molecular weight of between 2,800 and 3,000 Da through compatibility. Chemical and pharmacological experiments prove that: compared with single components subjected to compatibility, the complex can inhibit the generation of oxygen radicals caused by ultraviolet radiation obviously and can enter skin cell mitochondria in a targeting mode to a greater degree to inhibit light aging, and has the obvious protective effect on skin injury caused by the ultraviolet radiation. The complex provides theoretic support for the development of natural oligosaccharide-peptide complex uvioresistant and mitochondrion targeting medicaments and the high-value utilization of mariculture industry.
Owner:OCEAN UNIV OF CHINA

Tilapia fry rearing method

The invention discloses a tilapia fry rearing method. The tilapia fry rearing method comprises the following steps: breeding parent tilapia fries which are bigger than nine-sieve, picking parent tilapias which are disease free, injury free, strong and good in sexual maturity to rear, hybridizing the female parent tilapias and with the male parent tilapias according to ratio of stocking, picking up roes, incubating the roes with flowing incubating water in an incubating tank, cultivating the roes by marking in bold, and cultivating fries. Oviposition period is shortened by about seven days, incubating rate is as high as 67.9%, fry survival rate is 89.6%, each female tilapia lays 6700 fries each year averagely, fry production rate is improved by 30%, and the sizes of the fries are uniform. By means of the tilapia fry rearing method, fries can be reared all through a year, a large amount of tilapia fries are reared and the tilapia fries grow fast and are strong in stress resistance. The tilapia fries reared in mass are born at the same time, gonad developments are synchronous, sizes are the same, characters are unified, and heredity purity is high. Moreover, the tilapia fry rearing method has the advantages that incubating period is short, incubating efficiency is high, inheritable characters are good, fertility is strong, and fries can be reared in an overwintering mode.
Owner:化州市光辉养殖场有限公司

Special compound feed for tilapia meat quality improvement and preparation method thereof

The invention relates to the field of aquatic feed and particularly provides special compound feed for tilapia meat quality improvement and a preparation method thereof. The feed comprises the following components in parts by weight: 40-70 parts of broad bean, 2-5 parts of fish meal, 7-12 parts of bran, 9-19 parts of rapeseed mea1, 8-19 parts of peanut meal, 1-2 parts of fish oil, 0.3-0.5 part of glycine betaine, 0.4-0.6 part of choline chloride, 0.6-0.8 part of monocalcium phosphate, 0.10-0.13 part of methionine, 0.06-0.1 part of enzyme preparation, 0.02-0.03 percent of garlicin, 0.4-0.6 percent of compound vitamin and 1.0-1.2 parts of composite mineral salt. The invention also provides a preparation method of the feed. The feed has the benefits that the tilapia meat quality can be improved, so that tilapia meat is hard and crisp; and the growth performance of tilapia is improved, so that the pollution to the water quality is reduced, and the production cost is reduced, therefore, the economic and social benefits are remarkable.
Owner:福建省淡水水产研究所

Method for waterless keep-alive transportation by narcotizing tilapia mossambica with low-concentration carbon dioxide

InactiveCN104221976ASolve the problem of low loadSolve the high oxygen demandClimate change adaptationPisciculture and aquariaEngineeringEnvironmental engineering
The invention relates to a method for waterless keep-alive transportation by narcotizing tilapia mossambica with low-concentration carbon dioxide and belongs to the technical field of living fish waterless transportation. According to the method, the carbon dioxide serves as the anesthetic of the tilapia mossambica, the influences of the concentration of the carbon dioxide, the temperature of narcotizing liquid, the narcotizing time, the keep-alive temperature, oxygenation and other parameters on the waterless keep-alive time of the tilapia mossambica are studied, and the optimal tilapia mossambica waterless keep-alive transportation parameters are determined. After the method is applied, the tilapia mossambica can be kept alive for four to five hours, and the survival rate is 100 percent. According to the method, the transportation efficiency is improved, the transportation cost is reduced, and meanwhile the transported tilapia mossambica does not have the potential safety hazard of medicine residues.
Owner:GUANGDONG OCEAN UNIVERSITY +1

Special compound feed for tilapia

The invention relates to special compound feed for tilapia, which is prepared by the following raw materials in parts by weight: 3-8 parts of fish meal, 15-30 parts of soybean meal, 15-30 parts of rapeseed meal, 10-15 parts of cottonseed meal, 10-15 parts of DDSG (Distillers Dried Grains with Solubles), 10-20 parts of flour, 10-12 parts of corn, 4-8 parts of zeolite powder, 1-3 parts of soybean oil, 1-2 parts of fish oil, and 0.6-1.2 parts of special feed additive for the tilapia, and the sum of the parts by weight of the raw materials is 100 parts. The special compound feed for the tilapia can improve the economic benefit of tilapia culture distinctly, enhance the anti-stress capacity of the tilapia significantly, improve the immunity of the tilapia, and reduce the incidences of nutritive fatty liver diseases and streptococcicosis during the culture; a survival rate of the tilapia is increased by 16-20%; the growth of the tilapia is promoted; and the quality of fish is improved.
Owner:南通市通州区十总晨友农产品有限公司

Novel strain of bacillus coagulans as well as microecological preparation and feed containing bacillus coagulans

The invention discloses a bacillus coagulans strain capable of resisting streptococcus and applications of the bacillus coagulans strain. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and is assigned with the accession number of CGMCC No.12196. The strain is prepared into fungus powder which is added to an animal feed, the fungus powder also can beapplied as a microbial preparation for resisting streptococcus, and after the feed containing the strain is used for feeding tilapia mossambica, streptococcicosis can be effectively prevented and treated. The strain is mixed with bacillus subtilis and lactobacillus johnsonii, then a compound microbial preparation is prepared, and the compound microbial preparation can improve the growth performance of tilapia mossambica, and improve the activity of superoxide dismutase and catalase of the liver and lysozyme in serum. In addition, the fermentation broth of the strain can be adopted as a breeding environment modifier, after the fermentation liquor of the strain is sprayed to an aquaculture pond of tilapia mossambica, the quantity of streptococcus in the aquaculture water can be reduced, andnitrite in the water can be eliminated under the low oxygen condition.
Owner:BEIJING DABEINONG TECH GRP CO LTD +1

Method for producing calcium amino acid

The invention discloses a method for preparing calcium amino acid, including the preparation of a culture liquid which consists of fish bone powder and enzymolysis protein liquid and the inoculation of lactobacillus acidophilus and streptococcus thermophilus in the culture liquid, then a fermentation liquid which consists of the fish bone powder and the enzymolysis protein liquid is prepared; the mixture of the lactobacillus acidophilus culture liquid and the streptococcus thermophilus culture liquid are inoculated into the fermentation liquid for fermentation, and the fermentation liquid is treated with centrifugation to obtain a yellow calcium amino acid clear liquid. The invention takes tilapia processing offcut-fish bone as main raw material by the combination of the enzymolysis and microbial fermentation; the enzymolysis protein liquid which is prepared by enzymolysis is taken as a protein source, the calcium in the fish bone is freed to be combined with the amino acid in the enzymolysis protein liquid to form the ionic-state calcium amino acid by the fermentation of the lactobacillus acidophilus and the streptococcus thermophilus. The invention can not only high-value utilize the tilapia processing offcut, but also can improve the processing utilization ratio of the tilapia.
Owner:SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI

Tilapia surimi sausage having high gel strength, and its preparation method

The invention relates to a tilapia surimi sausage having a high gel strength, and its preparation method. A surimi heat sterilization process and additives are deeply researched to improve the formula and the processing technology of the tilapia surimi sausage and increase the gel strength of the tilapia surimi sausage. Optimized additive selection, gelating conditions, heating conditions and sterilization conditions are adopted, and the preparation method comprises the following operations: unfreezing frozen tilapia surimi, cutting and mixing, adding salt, cutting and mixing, adding a composite additive, cutting and mixing, adding ice to adjust the water content, filling a sausage, gelating, carrying out fish cake treatment, cooling, and carrying out heat sterilization. The tilapia surimi sausage prepared through the method can be preserved at normal temperature and also has a high gel strength, and the method provides an approach for the deep processing of other low-value freshwater fish surimi.
Owner:SOUTH CHINA UNIV OF TECH

Collagen polypeptide rich in glycine and formaldehyde scavenger prepared therefrom

The invention belongs to the technical field of functional polypeptides and discloses a collagen polypeptide rich in glycine and a formaldehyde scavenger prepared therefrom. The collagen polypeptide is prepared from the following steps: extracting collagens by means of a hot water extraction method; and then preparing the collagen polypeptide rich in glycine by enzymolysis of the collagens by means of protease by taking Tilapia mossambica waste as a raw material. By being combined with plant polyphenol compounds, the quick, long-acting, safe and edible formaldehyde scavenger is prepared. The formaldehyde removal rate of the formaldehyde scavenger can reach 95% or above within 10 min, so that the problem that a single amino acid is not ideal in removing formaldehyde is solved. A colloid film which is 2-10 microns can be formed on the surface of an object such as furniture if being dried to inhibit further release of formaldehyde. The formaldehyde removal rate can be kept at 95% or abovewithin three months, an effect of removing formaldehyde for a long time is achieved, and the problem that existing formaldehyde removal products are rebound in effect and short in effective period issolved.
Owner:SOUTH CHINA UNIV OF TECH

Rapid and efficient method for breeding tilapias

The invention relates to a rapid and efficient method for breeding tilapias, which is characterized by comprising the following steps of: utilizing GIFT strain Nile tilapias of 60 families introduced from the Malaysia Worldfish Center as a basic breeding colony; separating tilapia fries of different families into different net cages for breeding; respectively selecting 30 fries from each family, and injecting a radio frequency identification to a fry abdominal cavity with an injector to identify individuals; breeding the fries before a propagation season of the next year, and measuring body weight, body length, body height and body thickness data of all identified tilapias; estimating a breeding value of each fringetail according to a BLUP (Best Linear Unbiased Prediction) method by utilizing ASREML software; selecting male tilapias with the estimated breeding values of the top 60 and female tilapias with the estimated breeding values of the top 120, and carrying out nest mating propagation in the ratio of 1:2 of the male tilapia to the female tilapia to establish a family of the second generation; taking out oosperms from the oral cavity of the female tilapia which spawns, and transferring the oosperms into a hatcher to carry out hatching of running water. The rapid and efficient method for breeding the tilapias can avoid the problem of inbreeding depression caused by inbreeding, thereby being capable of carrying out breeding for a long period of time.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Recombinant oral protein TAT-GH of tilapia, preparation method for recombinant oral protein TAT-GH and application of recombinant oral protein TAT-GH

The invention discloses recombinant oral protein TAT-GH of tilapia, a preparation method for the recombinant oral protein TAT-GH and application of the recombinant oral protein TAT-GH. The method comprises the following steps of: extracting total messenger ribonucleic acid (mRNA) from a pituitary gland of male tilapia, performing reverse transcription-polymerase chain reaction (RT-PCR) to obtain complementary deoxyribonucleic acid (cDNA), performing PCR amplification to obtain a growth hormone (GH) gene of the tilapia, and amplifying a TAT-GH gene by a PCR overlap extension method; constructing a recombinant plasmid pET-32a(+)-TAT-GH; and transforming Escherichia coli BL21 (DE3) by using the recombinant plasmid pET-32a(+)-TAT-GH, efficiently expressing fusion protein TAT-GH in the form of an inclusion body under the induction of isopropyl thiogalactoside (IPTG), and purifying and renaturing to obtain active protein. The protein can effectively promote the growth and development of fries of the tilapia after being fed to the fries of the tilapia.
Owner:HUBEI TAIYANGHONG BIOLOGICAL TECH CO LTD

Flavor peptide and flavor peptide seasoning, and preparation method thereof

ActiveCN109329860AHigh umami intensityAdd flavorProtein food ingredientsMaillard reactionUmami
The invention discloses a flavor peptide and a flavor peptide seasoning, and a preparation method thereof. The flavor peptide comprises a polypeptide with an amino acid sequence of Lys-Gly-Ser-Leu-Ala-Glu-Glu-Glu-Glu. The preparation method is as follows: mashing Nile tilapia, adding proteolytic enzyme for enzymolysis, conducting centrifuging and concentration on the enzymolysis product, separating the concentrated solution with Sephadex G-15 chromatographic column, taking the third component, separating the third component with RP-HPLC, and taking the second component so as to obtain the flavor peptide. The preparation method of the flavor peptide seasoning is as follows: adding the prepared flavor peptide into a Maillard reaction base material, and carrying out Maillard reaction at 105-125 DEG C. The flavor peptide and the seasoning in the invention not only have delicious taste, but also have high nutritional value, and are ideal taste regulating products.
Owner:HENGYANG NORMAL UNIV

Method for extracting fishskin collagen polypeptide from fish wastes

The invention relates to a method for extracting fishskin collagen polypeptide by taking the wastes of such fishes as codfish, salmon or tilapia and the like as the raw materials, which comprises the processing steps of extracting single-spiral collagen, removing fishy smell and deodorizing, enzyme cutting, decolourization, desalting and spray drying; the fishskin containing natural collagen of triplehelix tight structure is washed, drained and then added into softened water for being extracted for three hours by controlling the pH value and the temperature condition, then the pH value is adjusted again and diatomite is added for coarse filtration, and the coarse filtrate is the single-spiral fishskin collagen; and the coarse filtrate is carried out decompress concentration and added with organic solvent for being mixed and stirred for 1 hour, and then rest, extracted and carried out fishy smell removing and deodorizing; and then an enzyme mixture is added to enzyme-cut the solution and a decoloring agent is also added for decoloring for 0.5 hour when the temperature control and stirring are carried out, the solution is then filtered; and finally, desalting and spray drying are carried out to obtain white fishskin collagen polypeptide powder products. The method has simple technical process, convenient operation and control, high utility ratio and good quality.
Owner:QINGDAO BETTER BIO TECH

Micro-organism preparation for preventing and curing streptococcicosis of Tilapia mossambica as well as preparation method and application thereof

The invention relates to a micro-organism preparation for preventing and curing streptococcicosis of Tilapia mossambica as well as a preparation method and an application thereof. The micro-organism preparation method for preventing and curing streptococcicosis of Tilapia mossambica comprises steps of compounding mixed vaccine with red Nocardia ATCC11653 and coral Nocardia ACCC40100, fermenting and cultivating the mixed vaccine with fermentation medium which is compounded of nitrogen source, carbon source and inorganic salt, and processing resultants into solid powder after the fermentation and cultivation. The micro-organism preparation for preventing and curing streptococcicosis of Tilapia mossambica, which provided by the invention, can reduce the content of ammonia nitrogen and nitrate nitrogen in the Tilapia mossambica breeding water body, antagonize the streptococcicosis of the Tilapia mossambica, reduce the accumulation of harmful substances of the middle and later breeding periods, enhance the immunity of the Tilapia mossambica,, achieve the aim of preventing and curing streptococcicosis of Tilapia mossambica, reduce the economic loss caused by diseases, and ensure the breeding output and quality of the Tilapia mossambica. The micro-organism preparation provided by the invention has advantages of convenience in use, small amount of usage and stable effect after use.
Owner:FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI

Application of fishery electrochemical water in transporting fish larvae of tilapia mossambica

ActiveCN104381166ABloodless redWater clarificationClimate change adaptationPisciculture and aquariaTilapiaFish larvae
The invention belongs to the field of fish larvae cultivation, and particularly relates to an application of fishery electrochemical water in transporting fish larvae of tilapia mossambica. The safe and environment-friendly fishery electrochemical water, which is without any pollution, bacteria, algae and insects, is used for transporting the fish larvae, and is used for transporting the famous fish larvae, such as the tilapia mossambica, so that the transportation survival rate and quality of the fish larvae can be effectively improved, the transportation time is beneficially prolonged, and the transportation capability is beneficially improved. The application and a method provided by the invention are simple and convenient in operation, become effective quickly, and have a wide application prospect.
Owner:TONGWEI
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