247 results about "Grass Carp (fish)" patented technology

The invention provides a feed for improving the disease resistance of a grass carp. The feed is characterized by comprising soybean cake, wheat meal, shell meal, fish meal, table salt, rice bran, fish oil, betaine, traditional Chinese medicine feed premix additive, zinc-containing polypeptide, zinc-containing microelement additive, vitamin mixture additive at least containing vitamin C, vitamin E and inositol, yeast and amino acid mixture additive at least containing threonine. The feed provided by the invention can improve the immunity of the organism of the grass carp and protect the grass carp from getting sick; the application of antibacterial chemical medicines and anti-virus chemical medicines is reduced to a certain degree, the survival rate of the grass carp is increased, and the activity of lysozyme and superoxide dismutase in the grass carp is improved; and moreover, the feed ensures little drug residue and low toxicity, and does not influence the quality of the grass carp or harm the human health.

The invention discloses a grass carp feed and a preparation method thereof. The grass carp feed is prepared by the following raw materials in parts by weight: 15-20 parts of bean pulp, 10-15 parts of rapeseed dregs, 5-10 parts of fish meal, 4-8 parts of rice bran, 15-20 parts of wheat middling, 8-12 parts of bran, 1-2 parts of an additive, 3-6 parts of beer yeast, 5-10 parts of corn proteins, 2-4 parts of locust tree leaves, 1-3 parts of sesbania powder, 1-2 parts of citrus peel powder, 1-2 parts of monocalcium phosphate, 0.1-0.2 part of edible salt, 2-3 parts of linseed oil and 1-2 parts of dai-dai flower oil. The grass carp feed is complete and balanced in nutrition, high in food calling property, high in palatability, high in digestion rate and complete in nutrition indexes and has various nutritious components required by the growth of grass carps. After being fed with the feed, the grass carps grow fast, the disease resistance rate is obviously increased, and the survival rate can be increased to be over 95 percent; therefore, the breeding cost is greatly reduced, and high-quality intensive breeding is realized.

The invention relates to a method for preparing three-classification whole blood control substance simulacrum and a blood type detection reagent box made by the method. The method comprises the preparation of red blood cell simulacrum, white blood cell simulacrum and blood platelet simulacrum. The concentration of the prepared red blood cell simulacrum, white blood cell simulacrum and blood platelet simulacrum is adjusted to ensure that cell particle is suspended in cell suspension and is used as monomial substance control substance, thereby forming whole blood control substance. The invention aims to solve the source of various cells and provides a method for preparing three-classification whole blood control substance. The invention adopts fish blood to simulate human white blood cell and blood platelet, and waste red blood cell of a livestock red blood cell plasma-mixing station to simulate human red blood cell, thereby simulating the practical histogram distribution of human blood sample; moreover, the cell suspension adopting the formula of the invention has longer storage life; in addition, the method can carry out simulation operation when adopting the red blood cells of silver carp, grass carp, common carp and crocodile, etc. during experiment, and can obtain excellent quality-control effect through fixative treatment.

The invention discloses a culture method of Tilapia. The culture method comprises the following steps of: selecting a pond which is 2 meters deep, and disinfecting the pond with unslaked lime after emptying the pond in late October every year; before late November, putting grass carp fries which are all heavier than 250g and feeding with a floating grass carp granulated feed when the water temperature is higher than 15 DEG C; in the next year, putting overwintering Tilapia fries which are all heavier than 200g when the water temperature of the pond is higher than 18 DEG C steadily, and feeding the Tilapia fries twice every day; in late July, fishing 90% of Tilapias for sale, then putting current Tilapia fries which are all heavier than 200g, feeding with a Tilapia floating feed containing 28% of protein in a feed ratio of 1-3%; and fishing all the fishes for sale in late October. By utilizing the culture method, the survival rates of Tilapia and grass carp are higher than 99% and 98% respectively, the yearly output of the pond is improved by 15%, and the commercial fishes are large and uniform in specification and are good in quality. The culture method is a healthy ecological culture mode mainly for culturing Tilapia.

The invention discloses a liquor-marinated fish processing technology which is characterized by comprising the following steps: (1) cleaning: fresh grass carps are taken and processed through removal of scales, heads, tails and internal organs, the thickness is within 1.5 cm, part of which the thickness is larger than 1.5 cm is lightly scratched by a knife, without damaging the skin, black films and bloodiness are cleaned, fish slices are uniform, and the optimal weight of the grass carp is less than 3 kg; (2) curing to deodorize: salt, cooking wine and curing material are weighed, one layer of curing material, salt and cooling wine is scattered on each layer of fish slice in curing, the quantity of salt on the bottom layer is small, while the quantity of salt on the top layer is large, the curing time is 7 days, and tank overturning every day is carried out once. The liquor-marinated fish has the characteristics of moderate taste, fresh meat, fragrant flavor and chewing feeling. The fish meat has the effects of invigorating stomach to remove coldness, boosting immunization, clearing away heat and toxic materials, preventing cancer, reducing blood fat, assisting resisting computer radiation, and whetting the appetite, is good to the physical fitness, and is suitable for being eaten by populations sitting in the office too long.

The invention relates to a feed Chinese medical herb composition, a feed additive, feed and a preparation method. The Chinese medical herb composition is prepared from frangula crenata Miq. powder, pine needle, wormwood, limonite, motherwort herb, tradescantia, lindera glauca, barley malt, dried crataegus pinnatifida and the like. The invention also provides the feed prepared by using the Chinesemedical herb composition or the feed additive and the preparation method thereof. The invention also provides feed additives, which are products of benefiting flora. In the invention, while the introduction of harmful residues is reduced, the immunity of aquatic livestock such as grass carp is improved, the growth of the livestock is speeded up and the quality of the aquatic livestock product such fish is improved; and in addition, the waste of field crop stalks and the like is made into valuable things, the dependence on grains is relieved, and environmental is protected.

A method for culturing one-instar fry of black carp, grass carp, silver carp and variegated carp includes choosing rarieties, pretreating pond, proportionally culturing them in a single pond, regulating water quality, and scientifically applying feed and fertilizer.

The invention discloses a compound feed for healthy aquaculture of grass carps in a large water area and a production method thereof. The compound feed is compounded from the following raw materials in parts by weight: 10-20 parts of bean cakes, 15-25 parts of rapeseed cakes, 10-20 parts of cotton cakes, 10-19 parts of wheat bran, 10-15 parts of wheat middling, 10-20 parts of rice bran, 3-6 parts of shell powder, 5-10 parts of wheat meal, 4-8 parts of straw powder, 0.1-0.2 part of southernwood powder, 0.01-0.05 part of dried medlar powder, 0.1-0.2 part of vitamin b complex, 10-20 parts of liquid lactobacillus, 20-40 parts of water, 0.1-0.5 part of salt and the like. The compound feed has the advantages that 1, the nutritional ingredients and mineral substances of the pellet feed are rich and replace the original fish meal, so that the cost is reduced, an animal albumen source is obtained, and the absorption utilization ratio is high; 2, the meat quality of the grass carps is improved, the growth rate of the grass carps after eating the feed can be improved by more than 8%, the production cost of a grass carp feed is reduced by more than 5%, and the culture cost of the grass carps is reduced by more than 10%; and 3, the immunologic function of the grass carps is improved, the capabilities of preventing diseases, curing diseases and resisting viruses of the grass carps are enhanced, and the recapture rate of the grass carps can be improved by 5%.

The invention provides a method for improving n-3 polyunsaturated fatty acid in the meat of grass carp. Cross bred Pennisetum alopecuroides are matched with a compound feed for fresh water fish sold in the market to feed the grass carps. The weight proportion of the dry matters matched with the compound feed and the cross bred Pennisetum alopecuroides is 1.1 to 1. The invention filtrates and discovers a certain plant which is rich in Alpha-linolenic acid, adds the plant with a certain proportion to feed phytophagy fish through scientific research and produces cheap fish meat which is rich in the n-3 polyunsaturated fatty acid through the biotransformation of the phytophagy fish.

The invention relates to a grass carp interleukin 1 beta gene and protein and a recombinant expression method thereof. The invention provides the grass carp interleukin 1 beta gene and the grass carp interleukin 1 beta protein coded by the gene. The recombinant expression method of the invention adopts homology cloning technology to clone and obtain the cDNA complete sequence of the grass carp interleukin 1 beta gene, uses the mature peptide sequence of the grass carp interleukin 1 beta gene to construct a prokaryotic expression vector and screens out a recombinant expression system used for efficiently expressing the grass carp interleukin 1 beta gene. The recombinant expression method of the invention comprises the following steps: 1. performing gene cloning; 2. constructing and screening the recombinant expression vector; and 3. expressing the recombinant protein, and separating and purifying the expression products. The beneficial effect of the invention is that the technical scheme of the invention can be used to perform the industrialized production of the recombinant interleukin 1 beta, and the recombinant interleukin 1 beta can be used in the theoretical research of the immunologic mechanism of fish.

The invention discloses a preparation method for a health-care mixed feed for poly-cultured grass carp and crucian. The feed comprises the following raw materials, by weight, 1.5-3.5% of imported fish meal, 1.5-3.5% of domestic fish meal, 7-9% of 46.5 cotton meal, 17-19% of 46 soybean meal, 10-12% of rapeseed meal, 3-5% of puffed soybean, 9-11% of corn germ cakes, 9-11% of domestic corn alcohol lees, 0-2% of oil residues (cakes), 1-3% of intestinal membrane protein powder, 14-16% of hard wheat, 4-6% of fine wheat bran, 4-6% of full-fat rice bran, 1-3% of soybean oil, 0.3-0.5% of stone powder, 1-3% of monocalcium phosphate, 0.0-0.2% of salt, 0.2-0.4% of 70% lysine sulphate, 0.01-0.03% of 98% methionine, 0.0-0.2% of betaine hydrochloride, 0.0-0.3% of choline chloride, 0.1-0.3% of fish multi-vitamin, 0.1-0.3% of fish multi-mineral, and 0.0-0.2% of fish microecologics. The feed can keep poly-cultured grass carp and crucian to grow fast in the middle and later periods and can raise growth performance obviously.

The invention discloses a grass carp reovirus S11 gene eukaryotic expression recombinant plasmid preparation method and application thereof in serving as nucleic acid vaccine, and belongs to the technical field of gene engineering and molecular immunology. The preparation method disclosed by the invention is characterized by comprising the steps of extracting viral genome RNA, performing reverse transcription to convert the viral genome RNA into cDNA, amplifying a corresponding DNA sequence out, constructing the DNA sequence to pcDNA-3.1(+) plasmid, converting Escherichia coli DH5alpha, screening out positive clone bacteria containing recombinant plasmid, culturing a lot of the positive bacteria and extracting recombinant plasmid S11-pcDNA3.1 contained in the bacteria. The recombinant plasmid is utilized as nucleic acid vaccine to perform intramuscular injection on the grass carps, and the nucleic acid vaccine enters the muscle cells and expresses VP35 protein of the grass carp reovirus in the muscle cells; thus, fish body immune cell proliferation is stimulated, antiviral related gene expression is up regulated, fish bodies are stimulated to generate antiviral antibodies, and capability of grass carps in resisting grass carp reovirus infection is effectively improved; furthermore, the grass carp reovirus S11 gene eukaryotic expression recombinant plasmid can be used for preventing a grass carp hemorragic disease caused by the grass carp reovirus in aquaculture.

The invention relates to a fishway inlet system and design method for inducing grass carp and a device for determining the flow rate of fish, comprising: a fishway and its fishway inlet arranged along the river flow direction, and one side of the fishway and the fishway inlet is close to each other On one bank of the river, the other side is provided with a fish-lure water flow channel and a fish-lure water flow channel outlet in the same direction as the fishway and the fishway inlet. The present invention only needs to set up one water flow for luring fish, and the water flow condition for luring fish can be created by coordinating the water flow for luring fish with the water flow for fishway. There are only two water flow parameters that need to be calibrated: the optimal flow velocity at the fishway inlet and the optimal flow velocity ratio between the outlet flow velocity of the fish-lure water flow channel and the fishway inlet flow velocity. Compared with other water flow fish luring methods, two water flow parameter values ​​are clearly given. The water flow form of the fishway inlet is simple and the engineering quantity is small, not only can be directly used in the hydraulic design of the grass carp fishway inlet, but also can be easily extended to the fishway inlet water flow design of other fish species.

The invention relates to a compound feed for high-density cultivation of grass carps. The compound feed comprises the following raw materials in weight percentage: 15-25% of wheat shorts, 10-25% of distillers dried grains and soluble DDSG, 3-8% of fish meal, 8-18% of bean pulp, 7-15% of peanut meal, 5-12% of rapeseed meal, 5-10% of full-fat rice bran, 5-8% of meat and bone meal, 2-5% of zeolite powder, 1-3% of soybean oil, 0.8-2% of special feed additive for the grass carps, 0.2-0.3% of compound vitamins, 0.2-0.3% of compound microelements and 0.5% of table salt; and the sum of the weight percentages of all the raw materials is 100%. With the adoption of the compound feed for the high-density cultivation of the grass carps disclosed by the invention, the economic benefits of the cultivation of the grass carps can be obviously improved, four diseases of grass carp in a grass carp cultivation process can be obviously improved, and the survival rate is improved by 7-9%; and the feed conversion rate in the grass carp cultivation process can be effectively improved and the growth of the grass carps is accelerated.

A preparation method of a tea-flavor instant grass carp comprises the following steps: selecting grass carp raw materials, pretreating, vacuum preserving, tea soaking, deep frying, seasoning, vacuum packaging and sterilizing. The tea-flavor instant grass carp not only has the fish flavor but also has the tea flavor due to soaking, seasoning, frying, curing, and composite flavor enhancing. The tea-flavor instant grass carp is a healthy, nutrient and delicious instant product.

The invention provides a grass carp cultivation method comprising the following steps: (1) fry cultivation is carried out, namely grass carp fries are added into a cultivation pond, and are cultivated into fingerlings; (2) fingerling cultivation is carried out, namely the grass carp fingerlings are cultivated until an average mass of the fingerling reaches 500-1000g per fingerling; (3) adult fish cultivation is carried out, namely broad beans are used for feeding the grass carp fingerlings twice per day until the grass carp fingerlings grow into adult grass carps; and the adult grass carps with an average mass of 1000-3000g per carp can be caught and marketed. The grass carp cultivation technology provided by the invention has high efficiency, and assists in effectively improving grass carp annual yield and reducing feeding cost. Also, with the method, the adult grass carps have robust body constitution, good shape, compact meat and rich nutrients. The method meets the requirements of ecological fishery development.

The invention relates to a grass carp fish seed period vitamin premix compound and a preparation method thereof, and belongs to the technical field of a feed additive used for the fish breeding. The vitamin premix compound is prepared by mixing vitamin A, vitamin D, vitamin E, vitamin K3, vitamin B1, vitamin B2, nicotinic acid, calcium pantothenate, vitamin B6, vitamin B12, folic acid, biotin, phaseomannite, vitamin C and bean meal powder according to a certain percentage, and added in the feed according to 0.5 percent of proportion. The vitamin premix compound solves the problems that the mixture ratio and the dosage of the existing vitamin premix compound do not fit to the grass carp, a grass carp grows slowly by using the feed to breed the grass carp in the fish seed period, and the coefficient of the feed is high, etc. The vitamin premix compound has accurate mixture ratio and reasonable material selection, meets the needs of the grass carp in the fish seed period for the vitamin, improves the special growth rate and the rate of live weight growth of the grass carp, and the survive rate of the of the grass carp parr, has low feed coefficient and low breeding and manufacturing costs, and is applicable to feed the grass carp under one year in the fish seed period.

The invention relates to a processing method of a surimi product, and in particular relates to an animal and plant compound protein surimi product and a production method thereof. The surimi product comprises the main raw materials of grass carp surimi and eel surimi and further comprises the following raw material components in the total quantity of the grass carp surimi and the eel surimi: 15%-25% of minced pork, 1.5%-3.0% of salt, 0.2%-0.5% of phosphate, 5%-8% of soybean protein isolate, 5%-8% of wheat gluten, 8%-15% of corn starch, 5%-10% of potato starch, 0.2%-0.5% of carrageenan, 1%-2% of monosodium glutamate, 2%-3% of white granulated sugar and 0.5%-1.0% of ginger powder. The surimi product is produced through the processes of killing, cleaning, slicing, fish odor removing, rinsing, dehydrating, mincing, chopping, kneading, mixing, chopping, forming, quick freezing and the like of fresh fishes. According to the method, marine fish and grass fish with low values are combined with vegetable protein, so that the surimi product is balanced and rich in nutrition and delicious in taste, has smooth and crispy mouthfeel, and leads a person to endless aftertaste.

The invention relates to a comprehensive technical scheme for regulating submerged plant communities by utilizing herbivorous fish. The comprehensive technical scheme comprises the following steps: (1) selecting pioneer submerged plants and colonized submerged plants, and alternately planting; (2) calculating total biomass of to-be-cleared pioneer plant when the ratio of transparence to water depth is more than or equal to 0.8, stocking a corresponding number of grass carp according to the fact that each kilogram of grass carp is used for clearing away 600g of submerged plants every day; (3) clearing away grass carp when the height of pioneer submerged plants is accordant to that of the colonized submerged plants; (4) calculating the biomass of the to-be-controlled submerged plants when the height of to-be-colonized submerged plants exceeds the requirement, and stocking a corresponding number of megalobrama amblycephala according to the fact that each kilogram of megalobrama amblycephala is used for clearing away 400g of submerged plants every-day; and (5) clearing away megalobrama amblycephala when the height of the colonized submerged plants is controlled to 50-80 percent of the original height. The method is favorable to establishing and managing water-quality improvement and optimized landscape effect-based submerged plants in a small shallow lake, so that the water quality and landscape effect are ensured to keep an excellent state for a long time.

The invention discloses a method for preparing a chelerythrine medicament and application thereof in prevention and treatment of bacterial diseases of aquatic animals. The medicament consists of chelerythrine and starch serving as an auxiliary material. The method for preparing the prevention and treatment agent comprises the following steps of: weighing chelerythrine extract (the purity is 80 percent) and starch serving as the auxiliary material, adding the chelerythrine extract and the starch into a mixer, and mixing the chelerythrine extract and the starch uniformly to obtain a preparation containing 5 to 20 percent chelerythrine. When the bacterial disease prevention and treatment agent is added into feed for feeding, the dosage is 200 to 250 mg / kg (according to the quantity of the chelerythrine for each kilogram of weight); the prevention and treatment agent added in the feed has low toxicity, does not affect the quality of aquatic products, has no residue or pollution, is not easy to generate medicament resistance, does not affect the feed and growth of the aquatic animals, has low dosage, and is widely applied in prevention and treatment of bacterial hemorrhage diseases of mandarin fish, weever, carp, grass carp, eel, porgy, flounder and the like.

The invention discloses a pond natural ecological cultivation method of swimming crabs. Including the setting of aquaculture ponds, preliminary preparations for aquaculture, placement of aquaculture seedlings, treatment in the middle and late stages of aquaculture, daily management of ponds, and fishing processes, among which aquaculture seedlings also include stocking of shellfish seedlings and portunus aquaculture. Stocking of swimming crab seedlings, grass carp and shrimp seedlings in the pond. The present invention adopts multi-culture pond multi-species culture mode, and adopts connected symbiotic water system to improve the ecological recycling of nutrients in the water system; and, according to the growth process of swimming crabs, gradually add cultured crayfish and grass carp after they are dead. Swimming crab breeding ponds are used as food to improve the growth vitality of swimming crabs, increase the growth rate of swimming crabs, and reduce disease and death.

The invention provides a bait formulation specially used for fishing grass carp, consisting of silkworm pupa, maize flour, white sugar, bran, rice wine, rapeseed cake, soybean meal and perfume; the bait formulation is prepared by the steps as follows: the silkworm pupa, the maize flour, the bran, the rapeseed cake and the soybean meal are put in a container, fermented and taken out after 30 days; the rice wine, the white sugar and the perfume are put in the mixture. The bait formulation is researched and prepared according to the characteristic of the grass carp, adopts the channel material with fragrant and sweet tastes and can rapidly attract the grass carp. Furthermore, the cost is low and the effect is obvious.

A method for preparing high-quality low-salt minced grass carp comprises the following steps: pretreatment of grass carp, separation of fish meat, rinsing, beating, fine filtering, dehydrating, addition of a small amount of salt, chopping, sausage stuffing, implementation of an ultrasound-microwave technology to increase the quality of minced grass carp, cooling, packaging, and storage of the carp at -20 DEG C. The addition amount of salt is 0.5-1% so that the minced grass carp belongs to the low-salt minced fish and meets the health requirements of modern consumers; the minced grass carp is heated by the ultrasound-microwave technology, so that the gel intensity of the products is high, the water holding capacity is good and the chewiness is good; and the method has an important meaning for deep processing of grass carps and increasing of the addition value of grass carps, and has very great application prospect.

The invention discloses a universal fixed feed for grass carp and tilapia parent fish and a preparation method thereof and aims at providing a fixed feed for improving nutrition, constitution, reproductive capacity of the grass carp and tilapia parent fish, improving egg cell fertility rate, hatching rate and quality and survival rate of larval and juvenile. The universal fixed feed is composed of the following raw materials including 4-10 parts of fish meal, 1-7 parts of shrimp meal, 25-45 parts of soya bean meal, 3-8 parts of corn gluten meal, 15-25 parts of high protein flour, 2-8 parts of dried brewer's grain, 3-8 parts of mixed oil, 1-4 parts of squid ointment, 1-5 parts of soya bean lecithin, 1-3 parts of monocalcium phosphate, 0.1-2.0 parts of fine bran, 0.2-0.6 part of vitamin premix, 1-4 parts of mineral substance premix, 0.01-0.1 part of vitamin E, 0.1-0.6 part of vitamin C acetate, 0.02-0.06 part of astaxanthin, 0.1-0.3 part of choline chloride, 0.01-0.06 part of yeast cell wall, 0.02-0.06 part of antioxidant and 0.02-0.06 part of mildew preventive.

The invention relates to a brittleness grading method for crisped grass carp based on living body detection, comprising the following steps of: (1) collecting blood samples of crisped grass carp fromdifferent embrittlement stages and blood samples and muscle samples of grass carp in the same batch, pretreating the muscles, and tasting and identifying the brittleness level; (2) detecting alkalinephosphatase activity, complement C3 content, and lymphocyte content in blood samples of blood samples, and establishing a blood index database of crisped grass carp mites; (3) Establishing a non-standardized Bayes discriminant function based on blood indicators; (4) using the Bayes discriminant function to determine the brittleness level of the muscles of the crisped grass carp to be determined. The brittleness grading method for crisped grass carp based on living body detection realizes a method for detecting the brittleness of crisped grass carp by live fish, which can reflect and evaluate the embrittlement effect of crisped grass carp with objective and high accuracy without killing fish.

The invention relates to a feed additive for increasing immunity of mutton sheep and a preparation method thereof. The feed additive disclosed by the invention is characterized by being prepared from grass carp powder, alfalfa, calcium hydrophosphate, fish meal, mountain flour, aminophylline, choline chloride, lysine, methionine, complex vitamins, copper sulphate, ferrous sulphate, zinc sulphate, potassium iodide, sodium selenite, cobalt chloride, L-lactic acid, small peptide protein, casein, complex carbohydrate, oligosaccharides, bacillus licheniformis, bacillus subtilis, subprostrate sophora, chrysanthemum, pseudo-ginseng, eclipta alba, astragalus membranaceus, glossy privet fruit, almonds, dried tangerine or orange peel and bistort rhizome. The preparation method disclosed by the invention comprises the following steps: dissolving small peptide protein, casein, complex carbohydrate, oligosaccharides, bacillus licheniformis and bacillus subtilis in water at 30 DEG C, crushing an alfalfa and Chinese herbal medicine mixture, sieving the crushed mixture through a 80-mesh sieve, mixing the various components, and drying at 70-75 DEG C to obtain the mutton sheep feed additive. The feed additive disclosed by the invention is rich in nutrient substances and free from adverse effects to health of goats and any side effect, and is capable of increasing production performance, improving feed quality and promoting growth and breeding.

The invention relates to grass carp hemorrhage immunizing intensifier, specific bait, injection, and new immunity method. The invention aims at overcoming the defects existing in immunity and inoculability against the main diseases in the young fry stage. The immunizing intensifier is prepared by chitosan, beta-glucan, vitamin C and vitamin E. The weaning specific bait for the grass carp fry is prepared by using the above immunizing intensifier, rotifer, vitelline, and fecula to make preparative bait, mixing the preparative bait and relevant multi-vaccine fully to make the weaning bait for grass carp fry. Adding the immunizing intensifier of the invention in the hemorrhage bacterin can promote the immunity effect; by injecting the grass carp hemorrhage bacterin and the immunizing intensifier in the abdominal cavity of the female ripe fish 30-45 days before the grass carp parents propagating for primary pre-immunity, with the specific bait which is made from fry weaning bait using the rotifer and the like to adsorb the bacterin, the immunizing and preventing effect is strengthened, and the bacterin dosage is reduced. The invention solves the problems that the operation of immunity inoculability for the grass carp at the young fry stage is difficult and the effect is poor.

The present application disclosed a method for preparing a sterile transgenic fish, comprising constructing antisense RNA expression vector of salmon-type gonadotropin-releasing hormone, introducing the recombinant DNA fragment into carp oosperm by microinjection, and screening the sterile transgenic fish by Polymerase Chain Reaction and radioimmunoassay, wherein the expression vector comprising a promoter of carp beta actin (β-actin) gene, a complementary DNA fragment of antisense salmon-type gonadotropin-releasing hormone (sGnRH) gene from carp with 323 bp as a target gene comprising sGnRH decapeptide, the coding region of gonadotropin-releasing hormone associated peptide and 3′ non-coding sequence, and 3′ flanking sequence of grass carp growth hormone gene as a stop sequence. The method of the present invention is easy and convenient for operation which provides a basis for providing a technical platform of general applicability for solving the hereditary and ecological safety problems of transgenic fishes.

The invention relates to the aspect of aquaculture, in particular to a formula and production process of an environment-friendly and pollution-free grass carp feed. The formula includes a component A, a component B and a component C. According to the mass parts, 1-40 parts of bean pulp, 1-30 parts of peanut meal, 5-15 parts of wheat meal, 5-15 parts of corn flour and 5-10 parts of fish oil are evenly mixed to form the component A, 15-30 parts of a fermented feed, 10-25 parts of clostridium alcohol protein, 10-30 parts of chicken meal, 5-10 parts of earthworm powder, 1-5 parts of a liver and bile protecting additive and 1-5 parts of a decavitamin additive are evenly mixed to form the component B, and 20-30 kg of pulverized corn straw is put into 100 kg of starch water to be heated until viscosity is realized to form the component C. The raw material ratio in the process conforms to the feeding habit characteristic of grass carps, the production process is environmentally friendly and free of pollution, and the prepared fish feed cannot contaminate water.