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Method for preparing ACE inhibition peptide originate from fish skin

A technology of inhibitory peptides and fish skins, which is applied in the field of preparation of ACE inhibitory peptides derived from fish skins, can solve the problems of lack of depth, environmental pollution, unsystematic, etc., and achieve the effect of scientific and reasonable process, high safety and low cost

Inactive Publication Date: 2008-08-13
NANCHANG UNIV
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Problems solved by technology

If these leftovers are not used effectively, it will not only cause great waste, but also cause environmental pollution.
At present, the research on fish collagen in foreign countries is relatively in-depth, and there are many reports on the research on fish skin collagen. However, the research on tilapia fish skin collagen is neither systematic nor deep.

Method used

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  • Method for preparing ACE inhibition peptide originate from fish skin

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Embodiment example

[0032] Weigh 100.0008g of cleaned tilapia skin, after degreasing treatment, soak it in 700ml 0.1mol / L hydrochloric acid solution and 0.1mol / L sodium hydroxide solution for 1hr, wash with distilled water until neutral, and put it under the condition of 45℃ Homogenize with 1000ml of distilled water at constant temperature for 12 hours, extract the supernatant by suction filtration, raise the temperature to 50°C-55°C, adjust the pH value to 6.0, add 0.04671g of papain for enzymolysis at constant temperature for 2hr, and then place it in a water bath at a high temperature of 100°C Heated for 20 minutes to terminate the enzymatic hydrolysis reaction to obtain the enzymatic hydrolyzate of fish skin protein, and the degree of hydrolysis of tilapia fish skin protein was 12.3%.

[0033] The fish skin protein enzymatic hydrolyzate was debittered, decolorized and deodorized with a compound decolorizing agent (3g / L activated carbon yeast and cyclodextrin, etc.), and the supernatant was col...

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Abstract

A method for preparing ACE inhibition peptides from fish skin is provided. The method is prepared with tilapia skin, extracts fish skin albumen from tilapia skin, adapts prolease to hydrolysis through enzyme extinguishing, debitterizing, decolouring, de-flavoring, filtering, ion exchanging and concentrating to obtain fish skin proteolysis liquor, after untruly filter (cutoff molecular mass is 9000Da) the TSP-I, collect high activity component by SephadexG-15 gel chromatography, freeze and dry to obtain high activity ACE inhibition peptides. The invention has important meaning for satisfying integrated utilization of tilapia skin wastes and improving its attachment.

Description

technical field [0001] The invention relates to a preparation method of ACE inhibitory peptide derived from fish skin. Background technique [0002] Angiotensin-converting enzyme (ACE) plays an important role in the regulation of blood pressure. In KKS), ACE uses angiotensin I and bradykinin as substrates, causing vasoconstriction and causing blood pressure to increase. [0003] Angiotensin-converting enzyme inhibitors (ACEI) are the main effective drugs for the treatment of hypertension and heart failure today. The chemically synthesized ACE inhibitors have exposed many defects in clinical use, such as: short action time, easy blood pressure rebound, and harmful to human body. Damage to multiple parts of the body, etc. Angiotensin-converting enzyme inhibitory peptide, an ACE inhibitor derived from food protein, has attracted widespread attention because of its high food safety and no side effects. [0004] At present, antihypertensive peptides with ACE inhibitory activit...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/14
Inventor 刘成梅梁汉萦刘伟梁瑞红涂宗财
Owner NANCHANG UNIV
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