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35results about How to "Strong industrial implementation" patented technology
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Method for preparing antihypertensive peptides through enzymolysis of ground meat proteins of tuna
InactiveCN102808010AIncrease added valueRich sourcesPeptide preparation methodsFermentationChemical synthesisAlkaline protease
The invention discloses a method for preparing antihypertensive peptides through enzymolysis of ground meat proteins of a tuna. The method comprises the steps of: (1) regarding the ground meat of the tuna as the raw material, mixing and homogenizing the raw material and a buffer solution according to a weight / volume ratio of 1 g: 3-5 ml, and then adjusting the pH value to 9.5-10 to obtain a mixed solution; (2) heating the mixed solution to 55-60 DEG C for pre-heating, and adding alcalase according to 1.0%-1.5% of the raw material for enzymolysis at an enzymolysis temperature of 55-60 DEG C for 4-6 h; and (3) performing enzyme deactivation on the obtained enzymolysis product to obtain an enzymolysis solution and then enabling the enzymolysis solution to sequentially suffer from ultrafiltration, desalination, freeze drying and chromatography to obtain the antihypertensive peptides. The method for preparing the antihypertensive peptides through the enzymolysis of the ground meat proteins of the tuna, disclosed by the invention, has the advantages of abundant source of the raw material, low price, simple preparation process, relatively strong activity of the prepared antihypertensive peptides; compared with a chemosynthetic ACE (Angiotensin Converting Enzyme) inhibitor, the ACEP (ACE Inhibitive Bioactive Peptide) prepared through enzymolysis of the ground meat of the tuna has the advantages of being safe and nontoxic, specific in antihypertensive efficacy, easy to digest and absorb and the like.
Owner:ZHEJIANG OCEAN UNIV
Polyolefin/vegetable fiber composite material and preparation method thereof
The invention discloses a polyolefin / vegetable fiber composite material and a preparation method thereof. The composite material consists of polyolefin, vegetable fibers, functional modified parent stock and a processing assistant, wherein the weight percentage of the polyolefin is 20 to 60 percent; the weight percentage of the vegetable fibers is 40 to 80 percent; the weight of functional modified parent stock is 5 to 15 percent based on the total weight of the polyolefin and the vegetable fibers; and the weight of the processing assistant is 2 to 5 percent based on the total weight of the polyolefin and vegetable fibers. The preparation method of the composite material comprises: strictly drying the vegetable fibers, fully mixing the dried vegetable fibers and the processing assistant in equipment such as a high-speed mixer; blending the mixture with polyolefin particle materials and the functional modified parent stock, performing extruding granulation, and obtaining the composite material. Compared with the conventional polyolefin / vegetable fiber composite material, the composite material is obviously improved in impact toughness while retaining strength and modulus, and the application range of the polyolefin / vegetable fiber composite material is further enlarged.
Owner:成都芝田高分子材料有限公司
Method for improving survival ratio of lactobacillus after freeze drying by liquid nitrogen prefreezing
InactiveCN101503668AImprove survival rateEasy to prefreezeBacteriaMicroorganism based processesSludgeFreeze-drying
The invention discloses a method for improving the survival rate of lactobacilli after freeze drying by using prefreezing with liquid nitrogen, which comprises the following steps: (1) collecting well-cultivated bacterial sludge of lactobacilli and adding a protective agent according to a certain mixing ratio; (2) dripping the bacterial sludge into liquid nitrogen for prefreezing; (3) freeze drying the pre-frozen bacterial sludge; and (4) collecting product for storage under vacuum. The method of the invention effectively increases the number of live bacteria after freeze drying by replacing the conventional prefreezing method with prefreezing with the liquid nitrogen, and adopting the certain compound protective agent at the same time, is simple in related materials and convenient operation, and has high industrial implementation performance.
Owner:ZHEJIANG UNIV
Method for preparing ABP (antibacterial peptide) from porcine hemoglobin
InactiveCN105063144ANo pollution in the processNo side effectsPeptide preparation methodsFermentationHemolysisFreeze-drying
The invention discloses a method for preparing an ABP (antibacterial peptide) from porcine hemoglobin. The method comprises steps as follows: porcine blood corpuscles are obtained through separation of porcine whole blood and then subjected to hemolysis, enzymolysis, decoloration, ultrafiltration, vacuum concentration and freeze-drying, and the ABP is obtained; the dosage of trypsin is 3000 U / g, and the enzymolysis reaction time is 4-6 h under conditions that the pH is 8 and the temperature ranges from 45 DEG C to 55 DEG C. The method for preparing the ABP from the porcine hemoglobin is low in cost and good in effect.
Owner:CHONGQING DOUHAO BIOTECH
Elastic sound-absorbing cotton and preparation method thereof
InactiveCN103710866AImprove flatnessIncrease elasticitySynthetic resin layered productsSound producing devicesBoron nitrideEngineering
The invention discloses elastic sound-absorbing cotton composed of a top surface layer, a back surface layer and a cellucotton layer sandwiched between the top surface layer and the back surface layer. Both the top surface layer and the back surface layer are made of non-woven fabric materials. The elastic sound-absorbing cotton is characterized in that the non-woven fabric is composed of, by weight, 100 parts of isotactic polypropylene, 4-5 parts of calcium stearate, 2-3 parts of sodium chromate, 1-2 parts of sodium molybdate , 2-3 parts of sodium sulfate, 12-15 parts of dioctyl phthalate, 1-2 parts of dysprosium oxide, 1-2 parts of boron nitride, 12-15 parts of montmorillonoid, 10-12 parts of wheat straw, 3-4 parts of Cacumen biotae, 4-5 parts of Eclipta prostrata, 2-3 parts of kuh-seng, 1-2 parts of mastix and 4-5 parts of assistant, and is prepared by a melt blowing process. As the non-woven fabric is prepared by the melt blowing process, the method for preparing the elastic sound-absorbing cotton is simple, the raw materials of the elastic sound-absorbing cotton are environmentally friendly, cheap and easy to obtain, the elastic sound-absorbing cotton has the advantages of good evenness and excellent resilience, expanded function and application range as compared with the general sound-absorbing cotton, and the like.
Owner:芜湖跃飞新型吸音材料股份有限公司
Freeze-drying protective agent as well as preparation method and application method thereof
ActiveCN106754374AGain antifreeze propertiesLow costBacteriaMicroorganism based processesFreeze-dryingMicrobiology
The invention provides a freeze-drying protective agent as well as a preparation method and an application method thereof, in order to solve the problem on the preparation of the freeze-drying protective agent for different lactic acid bacteria strains in the prior art. According to the freeze-drying protective agent as well as the preparation method and the application method thereof disclosed by the invention, the freeze-drying protective agent is directly derived from corresponding lactic acid bacteria cells after freezing pretreatment at a low temperature, and is directly obtained by cell crushing treatment; and the freeze-drying protective agent disclosed by the invention has the advantages of easy availability for raw materials, simple preparation process, low cost, good freezing protection effect and stronger industrialized implementation.
Owner:ZHENGZHOU UNIV +1
Application of mussel protein antihypertensive peptide
InactiveCN104757561AStrong inhibitory activityIncrease profitPeptide/protein ingredientsFood preparationUltrafiltrationPharmaceutical Substances
The invention discloses an application of a mussel protein antihypertensive peptide. The antihypertensive peptide has antihypertensive activity and is characterized in that an amino acid sequence of the antihypertensive peptide is Val-Ser-Trp-Pro-Cys-Arg-Trp(VSWPCRW); the molecular weight determined by ESI-MS (electrospray ionization mass spectrometry) is 935.07Da. The active peptide Val-Ser-Trp-Pro-Cys-Arg-Trp is obtained by shelling mussels, taking the meat of the mussels, carrying out enzymolysis on the meat, carrying out ultrafiltration on the enzymatic hydrolysate, adsorbing the enzymatic hydrolysate with an agarose magnetic microsphere immobilized ACE (angiotensin converting enzyme) and carrying out purification by RP-HPLC (reversed-phase high-performance liquid chromatography). The active peptide prepared by the invention has obvious ACE inhibitory activity and can be used in hypertension treatment related drugs.
Owner:ZHEJIANG OCEAN UNIV
Method for improving lyophilized activity of Oenococcus oeni by utilizing stress pretreatment
InactiveCN102559500AImprove lyophilization activityImprove freeze-drying survival rateMicroorganism based processesWine preparationMonosodium glutamateAs Directed
The invention relates to a method for improving lyophilized activity of Oenococcus oeni by utilizing stress pretreatment, which comprises the following steps: performing enrichment culture of Oenococcus oeni strain to reach the stable period, centrifugally collecting the bacterial sludge, resuspending in the culture medium ATB containing the stress treatment fluid, standing at 25 DEG C for 2-3h, centrifuging, harvesting bacterial body, adding proper sodium pyruvate solution with mass concentration of 2-5% as the lyophilized protective agent and performing freeze drying to obtain lyophilized bacteria powder. The lyophilized bacteria powder prepared by the method of the invention can be used as direct Oenococcus oeni fermentation starter. The method of the invention introduces the idea of stress pretreatment, and uses sodium pyruvate as the lyophilized protective agent, which is the main component of the monosodium glutamate and is safe and no harm to human and poultry and has various health-care effects, the introduced idea can be used in the production process of direct edible fermentation starter.
Owner:NORTHWEST A & F UNIV
Method of utilizing duck blood to prepare antibacterial peptide
InactiveCN105112486AEfficient antibacterialNo pollution in the processPeptide preparation methodsFermentationAlkaline proteaseFreeze-drying
The invention discloses a method of utilizing duck blood to prepare antibacterial peptide. The method includes the following steps: hemolyzing duck blood; enzymolyzing; decoloring; performing ultrafiltration; performing vacuum concentration; freeze-drying to obtain antibacterial peptide. Adding quantity of alkaline protease is 5000U / g, and enzymolysis reaction time is 4-6h with pH to be 8 and at temperature of 45-55 DEG C. The method is low in cost and good in effect.
Owner:CHONGQING DOUHAO BIOTECH
Black fungus instant noodle seasoning powder
InactiveCN105212163AScientific and reasonable processing technologyEasy to operateFood scienceNutrientInstant noodle
The invention discloses a black fungus instant noodle seasoning powder and a processing technology thereof, and belongs to the technical field of food processing. According to the technology, black fungus powder is added into instant noodle seasoning powder, and black fungus can be used as an emulsifier. The effect of addition amount of black fungus on the color, taste, and physical states of instant noodle seasoning powder is researched. In the prior art, when the soup temperature is suitable for drinking, the oil starts to solidify, and the provided seasoning powder can solve the problem mentioned above. Moreover, black fungus has the functions of activating blood, removing stasis, boosting qi, strengthening immunity, moistening intestines, clearing lung, maintaining beauty, and protecting skin. The provided black fungus instant noodle seasoning powder is stable and is rich in nutrients.
Owner:NORTHEAST FORESTRY UNIVERSITY +3
Method for preparing antihypertensive peptide by using catfish bone
InactiveCN105063145ANo pollution in the processNo side effectsFermentationAlkaline proteaseNifedipine
The invention discloses a method for preparing antihypertensive peptide by using catfish bone. The method comprises the following steps: cleaning the catfish bone, stewing the catfish bone to obtain catfish bone paste, carrying out enzymolysis, decoloring, carrying out ultrafiltration and vacuum concentration, and freeze-drying so as to obtain antihypertensive peptide. According to the method, the enzyme dosage of alkaline protease is that the enzyme-substrate ratio is 500 U / g, and the enzymatic reaction time is 5-7 h under the conditions that pH is 8.5 and the temperature is 55-60 DEG C. The molecular weight of antihypertensive peptide prepared through the method provided by the invention is smaller than 5 kD, and the ACE inhibition rate of antihypertensive peptide reaches 88% or above and is higher than those of antihypertensive drugs nimodipine and nifedipine.
Owner:CHONGQING DOUHAO BIOTECH
Method for preparing blood fat reducing peptide by use of silkworm chrysalis
ActiveCN103540636BIt has the effect of lowering blood fatNo side effectsFermentationFiltrationUltrafiltration
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method for preparing antihypertensive peptide from hairtail backbone by fermentation method
InactiveCN101168762BInhibit ACE activityWith antihypertensive effectMicroorganism based processesFermentationBacillus licheniformisActivated carbon
The invention provides a method for preparing antihypertensive peptide by using ridge bones of hairtail in a fermentation way. The method comprises the following steps: (1), sugar, crushed hairtail ridge bone, peptone, K2HPO4, MgSO4.7H2O and water are mixed to produce fermentation medium, after sterilization, bacillus licheniformis is inoculated, and is cultured to obtain fermentation liquid; (2), after the fermentation liquid is centrifuged, the supernatant liquid is taken and filtered, and enzyme sterilization is performed to the filter liquor; (3), the filter liquor after the enzyme sterilization is centrifuged, discoloring and smell removal are performed to the centrifuged supernatant liquid by activated carbon, so as to obtain the filter liquor after the discoloring and the smell removal; (4), the filter liquor after the discoloring and the smell removal is ultrafiltered to obtain permeation liquid, freeze drying is performed to the permeation liquid after vacuum concentration, and then peptide powder with antihypertensive function is obtained. The sources of the raw material adopted by the method of the invention are rich, the price is low, the operation is easy, and the method has stronger industrialized practicability.
Owner:ZHEJIANG UNIV
A kind of lyoprotectant and its preparation method and use method
ActiveCN106754374BGain antifreeze propertiesLow costBacteriaMicroorganism based processesFreeze-dryingMicrobiology
The invention provides a freeze-drying protective agent as well as a preparation method and an application method thereof, in order to solve the problem on the preparation of the freeze-drying protective agent for different lactic acid bacteria strains in the prior art. According to the freeze-drying protective agent as well as the preparation method and the application method thereof disclosed by the invention, the freeze-drying protective agent is directly derived from corresponding lactic acid bacteria cells after freezing pretreatment at a low temperature, and is directly obtained by cell crushing treatment; and the freeze-drying protective agent disclosed by the invention has the advantages of easy availability for raw materials, simple preparation process, low cost, good freezing protection effect and stronger industrialized implementation.
Owner:ZHENGZHOU UNIV +1
Method for preparing bean germination functional peptide
ActiveCN105779550BInhibits HMG-CoA reductaseInhibits ACE enzyme a-glucosidase activityFermentationMicroorganismAlglucerase
A method for preparing bean sprout-derived functional peptide, comprising the following steps: sprouting beans until the length of sprouts is 0.5-1.0 cm, adding water, mashing and crushing; and adjusting the pH value of the obtained slurry to 6.5-8.0, and then adding neutral protease, papain and flavourzyme for enzymolysis under a stirring condition, the resultant enzymatic hydrolysate containing bean sprout-derived functional peptide. Ultrafiltration is performed on the resultant enzymatic hydrolysate by using an ultrafiltration membrane, and the ultra-filtered enzymatic hydrolysate is maintained at a temperature of 70-80˚C, or ozone water having a concentration of 20-25 mg / L is used to avoid microbial spoilage; and then, spray drying is performed to obtain the bean sprout-derived functional peptide.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method for preparing antihypertensive peptides by using enzymatic degradation on mussel-digested protein
ActiveCN101948899BNo side effectsInhibit ACE activityPeptide preparation methodsFermentationAngiotensin-converting enzymeFiltration
The invention discloses a method for preparing angiotensin converting enzyme inhibitory peptide (ACEIP) by using enzymatic degradation on mussel-digested protein, which comprises the following steps: 1) mixing defatted dry mussel powder or mussel protein powder serving as raw material with water, then adjusting the pH value to 9.0 to 9.5 so as to obtain a mixed liquor; 2) raising the temperature of the mixed liquor to 60 to 65 DEG C for preheating, then adding alkali protease into the mixed liquor to carry out zymolysing reaction, wherein the zymolysing temperature is 60 to 65 DEG C and the zymolysing time is 180 to 240 minutes; and 3) carrying out enzyme inactivation processing on zymolysing products obtained in step 2 firstly so as to obtain zymolysing liquid; then sequentially carryingout ultra-filtration, freeze drying and chromatography on the obtained zymolysing liquid so as to obtain the ACEIP. The ACEIP prepared by using the method of the invention has the characteristic of strong activity.
Owner:ZHEJIANG GONGSHANG UNIVERSITY
Method for enhancing workability of concrete
The invention provides a method for enhancing workability of concrete. A technical scheme of the invention is that a workability improving agent with hydrophilic colloid as a main component is added into the concrete to enhance workability of the concrete. Adding the workability improving agent with hydrophilic colloid as the main component into the concrete can reduce sensitivity of water reducer to aggregate, improve concrete holding phenomenon during strong concrete pumping, reduce concrete pumping pressure, so as to endow better construction performance on the concrete.
Owner:SHANGHAI TAIJIE CHEM
A kind of purification method of hazelnut shell brown pigment
The invention discloses a method for purifying a hazelnut shell brown pigment. A D101 type macroporous resin is employed for secondary purification on a crude extract of the hazelnut shell brown pigment, and the method includes resin adsorption, distilled water leaching, ethanol desorption, concentration and drying, so as to obtain purified hazelnut shell brown pigment. The macroporous resin method for purification of hazelnut shell brown pigment provided by the invention has the advantages of simple operation, safety and no toxicity, provides technical support for the development and utilization of the hazelnut shell brown pigment and provides a high quality natural raw material for the development of functional food and food additives, and truly realizes safe, simple, reasonable, economical and high value utilization of agricultural and forestry resources and industrialized production of series products.
Owner:哈尔滨丹彤生物科技有限公司
Method for preparing hypoglycemic peptide by using Flammulina velutipes
ActiveCN107177653BHas an adjuvant therapeutic effectStrongly inhibits a-glucosidase activityHydrolysed protein ingredientsMetabolism disorderNeutral proteaseEnzymatic hydrolysis
The invention discloses a method for preparing blood glucose reducing peptides from flammulina velutipes. The method includes steps of mixing flammulina velutipes dry powder and sodium hydroxide aqueous solution with the mass concentration of 0.4%-0.6%, then carrying out water bath treatment, regulating a pH (potential of hydrogen) value of supernatant until the pH value of the supernatant reaches 4+ / -0.5, and then freezing and centrifuging the supernatant to obtain precipitates; adding water and neutral protease into the precipitates, carrying out enzymatic hydrolysis reaction under oscillation conditions, preserving heat of obtained enzymatic hydrolysis reaction products at the temperatures of 95-100 DEG C for 10-15 min, then naturally cooling the enzymatic hydrolysis reaction products until the temperatures of the enzymatic hydrolysis reaction products reach the room temperature and centrifuging the enzymatic hydrolysis reaction products to obtain enzymatic hydrolysate; carrying out ultra-filtration treatment on the enzymatic hydrolysate to obtain ultra-filtration enzymatic hydrolysate; carrying out spray drying on the ultra-filtration enzymatic hydrolysate to obtain blood glucose reducing peptide powder from the flammulina velutipes.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Method for purifying hazelnut shell brown pigment
The invention discloses a method for purifying a hazelnut shell brown pigment. A D101 type macroporous resin is employed for secondary purification on a crude extract of the hazelnut shell brown pigment, and the method includes resin adsorption, distilled water leaching, ethanol desorption, concentration and drying, so as to obtain purified hazelnut shell brown pigment. The macroporous resin method for purification of hazelnut shell brown pigment provided by the invention has the advantages of simple operation, safety and no toxicity, provides technical support for the development and utilization of the hazelnut shell brown pigment and provides a high quality natural raw material for the development of functional food and food additives, and truly realizes safe, simple, reasonable, economical and high value utilization of agricultural and forestry resources and industrialized production of series products.
Owner:哈尔滨丹彤生物科技有限公司
Preparation of depressor peptide by silkworm chrysalis
InactiveCN1896267BInhibit ACE activityWith antihypertensive effectFermentationCardiovascular disorderHydrolysateNeutral proteinase
The present invention discloses a method of preparing antihypertensive peptide from silkworm pupa which includes the following steps: the defatted dry powder of silkworm pupa or silkworm pupa protein powder is mixed with water at the mass ratio of 1:6-7, the pH value is adjusted to 6.0-6.5, then neutral proteinase is added for hydrolysis at 50-55DEG C for 120-200 minutes, the result hydrolysate is antihypertensive peptide. The weight of the said neutral proteinase is 0.9-1.1% of the defatted dry powder of silkworm pupa or silkworm pupa protein powder. The method in the present invention that utilizes silkworm pupa to prepare antihypertensive peptide has lower cost and better effect.
Owner:ZHEJIANG UNIV
Preparation method of hazelnut shell brown pigment
The invention discloses a preparation method of hazelnut shell brown pigment, which comprises the following steps that: two to six times of 0.5 to 3 percent sodium chloride water solution is added to be uniformly mixed after hazelnut shell is smashed, the temperature is adjusted to 30 to 50 DEG C, 1 to 4 percent sodium hydroxide solution with the volume being two to six times of that of the original solution after the temperature is maintained for 40 to 80 minutes, the temperature is adjusted to 50 to 70 DEG C and is continued to be maintained and continuously mixed so as to perform the extraction for 2 to 3 hours, then the extracted liquid is cooled, the pH value is adjusted, the extracted liquid is concentrated after being centrifuged, absolute ethyl alcohol is added into the extracted liquid to be centrifuged, the upper clean liquid is vacuum concentrated, is purified by macroporous resin, is concentrated, frozen and dried to obtain the brown powder-shaped hazelnut shell pigment. The source of the raw material adopted in the method is abundant, and the raw material is sourced from the waste of the agriculture and forestry. Through the technical conversion of the method, the economic added value of the waste such as the hazelnut shell can be effectively improved, thereby having important significance on promoting the development of the hazelnut production and processing industry.
Owner:王振宇 +1
A kind of vacuum low temperature continuous drying method for plant lactic acid bacteria
ActiveCN104277973BImprove survival rateContinuous productionMicroorganism based processesMicroorganism preservationBiotechnologyLactic acid bacterium
The invention discloses a vacuum low-temperature continuous drying method for lactobacillus plantarum. The method comprises the following steps of (1) adding a drying protectant into a lactobacillus plantarum solution to prepare a uniform mixture; (2) feeding the uniform mixture obtained in the step (1) to a vacuum low-temperature continuous drying machine with the model of MJ103-6; and (3) carrying out vacuum storage on bacterial powder obtained in the step (2). The vacuum low-temperature continuous drying method can be used for effectively increasing the survival rate of lactobacillus in the drying process and realizing large-scale production and has relatively strong industrial feasibility.
Owner:LINZHOU SINAGRI YINGTAI BIOLOGICAL PEPTIDES CO LTD
A method for facility farmland and facility farmland
ActiveCN106965708BReduce harmHigh outputPhotovoltaic supportsPV power plantsElectric power transmissionSpatial positioning
A photovoltaic system comprises a rack, a photovoltaic assembly, a set of rails, and a multifunctional robot operating on the rails. The photovoltaic system also comprises a fixed power source and more than two power transmission buses. The power transmission buses output power by using a sliding power taking manner, and comprise metal conductors on the rack and the rails. The fixed power source, the power transmission buses, an electric brush and a power consumption load of the multifunctional robot form a loop. The fixed power source continuously supplies power. In the prevent invention, rails are disposed on the rack of the photovoltaic assembly, and power is transmitted by using the rails, thereby providing the multifunctional robot with a comprehensive platform for spatial positioning, mobile positioning and power transmission. With the rail change machine, no person is needed on the site of the photovoltaic system. Functional components of the multifunctional robot comprise an agricultural machinery device, thereby providing a new solution for the agriculture, and resolving the site problem of photovoltaic power generation by using a manner friendly to the agriculture. An automatic electric plug device provides a new means for taking power by the agricultural robot in movement.
Owner:ANHUI JIULI MACHINERY EQUIP
Rapid preparation method of mussel protein antihypertensive peptide
ActiveCN104762358BStrong inhibitory activityIncrease profitOther chemical processesPeptide preparation methodsUltrafiltrationPharmaceutical Substances
Owner:山东大树达孚特膳食品有限公司
Method for producing anti-hypertension peptide with complex zymohydrolysis of hairtail backbone
InactiveCN101153310BInhibit ACE activityWith antihypertensive effectFermentationFish material medical ingredientsHydrolysateAnti hypertension
The invention discloses a method for producing angiotensin converting enzyme inhibitory peptide with combined catalysis on hairtail chine, which includes the following procedures that: the hairctail chine is shattered and mixed evenly with water, with PH regulated and the temperature raised to 30-45 DEG C, added with elastase to undergo enzymatic hydrolysis, then the temperature of the hydrolysate liquid is raised rapidly to 90-100 DEG C and kept for 10-15 minutes, then cooled down rapidly to the room temperature to terminate the reaction, then the pH of the hydrolysate liquid is regualated to 1.5-3.0, which is added with pepsin for enzymatic hydrolysis, then the temperature of the reaction system is rapidly raised to 90-100DEG C, kept for 10-15 minutes, and cooled down to the room temperature rapidly to terminate the reaction, the hydrolysate liquid is centrifuged, and the supernate is harvested, condensed in vacuum, refrigerated and dried, to obtain the power-like angiotensin converting enzyme inhibitory peptide. The raw material of the invention is rich in raw material but low in price, and the technical transformation provided by the invention can improve the appendix value of hairtail chine as the residue material of processed marine products, and has importance to go on enhancing the development of the industry of processing of marine products.
Owner:ZHEJIANG UNIV
Mussel Protein Antihypertensive Peptide
ActiveCN104945471BStrong inhibitory activityIncrease profitPeptidesCardiovascular disorderUltrafiltrationPharmaceutical Substances
The invention discloses a mussel protein antihypertensive peptide with an antihypertensive activity as well as a preparation method and an application thereof. The amino acid sequence of the antihypertensive peptide is Val-Ser-Trp-Pro-Cys-Arg-Trp (VSWPCRW), and the molecular weight measured by ESI-MS is 935.07 Da. The active peptide Val-Ser-Trp-Pro-Cys-Arg-Trp (VSWPCRW) is obtained through shelling and meat taking, enzymolysis, ultrafiltration, agarose magnetic microsphere immobilized ACE adsorption and reversed-phase high-performance liquid chromatography (RP-HPLC) purification. The active peptide prepared by the preparation method disclosed by the invention is remarkable in angiotensin converting enzyme (ACE) inhibitory activity, and capable of being used for hypertension treatment-related medicines.
Owner:安徽盛美诺生物技术有限公司
Method for preparing hypoglycemic peptide from silkworm chrysalis
The invention discloses a method for preparing hpyerglycemic peptide by using silkworm chrysalis. The method comprises the following steps: 1) adding water into degreased dried silkworm chrysalis powder, carrying out uniform mixing through stirring, then adjusting a pH value to 3.8 to 4.2, using the obtained mixture as a reaction starting substance, adding acidic protease into the reaction starting substance at 35 to 40 DEG C, carrying out a heat-preserved enzymatic hydrolysis reaction for 3.5 to 4.5 h under stirring and carrying out centrifugation after termination of the reaction so as to obtain a supernatant which is an enzymatic hydrolysis liquid; and 2) decoloring the enzymatic hydrolysis liquid with decoloring active carbon at 35 to 40 DEG C, subjecting the decolored enzymatic hydrolysis liquid to filtering so as to obtain a roughly filtered enzymatic hydrolysis liquid, carrying out ultrafiltration on the roughly filtered enzymatic hydrolysis liquid by using a hollow fiber polysulfone ultrafiltration membrane with a molecular weight cutoff of 3 Kda, concentrating the obtained ultrafiltered enzymatic hydrolysis liquid by using a vacuum thickener to a weight 25 to 40% of the original weight of the ultrafiltered enzymatic hydrolysis liquid and carrying out vacuum freeze drying so as to obtain peptide powder with a hpyerglycemic function.
Owner:ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
High-concentration compound microorganism bacterial fertilizer and production method thereof
InactiveCN101851123BImprove physical and chemical propertiesImprove fertilityOrganic fertilisersBacillus licheniformisHigh concentration
Owner:黑龙江亩佳科技发展有限公司
Method for improving survival ratio of lactobacillus after freeze drying by liquid nitrogen prefreezing
InactiveCN101503668BEasy to prefreezeEasy to operateBacteriaMicroorganism based processesFreeze-dryingSludge
The invention discloses a method for improving the survival rate of lactobacilli after freeze drying by using prefreezing with liquid nitrogen, which comprises the following steps: (1) collecting well-cultivated bacterial sludge of lactobacilli and adding a protective agent according to a certain mixing ratio; (2) dripping the bacterial sludge into liquid nitrogen for prefreezing; (3) freeze drying the pre-frozen bacterial sludge; and (4) collecting product for storage under vacuum. The method of the invention effectively increases the number of live bacteria after freeze drying by replacing the conventional prefreezing method with prefreezing with the liquid nitrogen, and adopting the certain compound protective agent at the same time, is simple in related materials and convenient operation, and has high industrial implementation performance.
Owner:ZHEJIANG UNIV
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