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Method for preparing antihypertensive peptides by using enzymatic degradation on mussel-digested protein

A technology of hypotensive peptides and mussels, applied in the field of bioengineering, can solve the problems of low activity of antihypertensive peptides ACE inhibitory peptides, etc., and achieve the effects of strong industrial implementation, improved utilization, and low price

Active Publication Date: 2012-08-15
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently known methods for preparing ACE inhibitory peptides from clams and oysters are not suitable for preparing hypotensive peptides from mussels (the reason is that the activity of the obtained ACE inhibitory peptides is low)

Method used

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  • Method for preparing antihypertensive peptides by using enzymatic degradation on mussel-digested protein
  • Method for preparing antihypertensive peptides by using enzymatic degradation on mussel-digested protein
  • Method for preparing antihypertensive peptides by using enzymatic degradation on mussel-digested protein

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Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1. A method for enzymatically hydrolyzing mussel protein to prepare hypotensive peptides, the following steps are performed in sequence:

[0044] 1), prepare defatted dry mussel powder:

[0045] Wash the fresh blue mussels with water, shell and remove the silk, homogenize them with a beater until they are paste, freeze-dry (-50°C, 72 hours) to obtain mussel powder. Add the mussel powder at 40°C to degrease the ethanol solution with a volume concentration of 95% in the ratio of 1g:4ml for 4h, repeat three times; vacuum-dry the above-mentioned degreased mussel powder at 40°C (5 hours), pulverize And pass through a 100-mesh sieve to obtain defatted dried mussel powder with a moisture content of ≤2.34%.

[0046]2) Weigh 5.0 g of the above degreased dried mussel powder (protein weight content 60.23%) in the enzyme reactor, add 100 ml of deionized water and stir evenly, homogenize for 10 min, adjust the pH to 9.2 with 0.5 mol / L NaOH solution ; A mixture was obtai...

Embodiment 2

[0058] Embodiment 2, a method for enzymatically hydrolyzing mussel protein to prepare hypotensive peptides, the following steps are carried out in sequence:

[0059] 1), prepare defatted dry mussel powder:

[0060] Replace fresh blue mussel with fresh thick shell mussel, all the other are the same as embodiment 1.

[0061] 2) Weigh 5.0 g of defatted dried mussel powder (protein weight content 72.56%) into the enzyme reactor, add 100 ml of deionized water and stir evenly, homogenize for 10 min, and adjust the pH to 9.2 with 0.5 mol / L NaOH solution; Get a mixture.

[0062] 3), the temperature of the mixed solution was raised to 60° C., stirred and preheated, and kept under stirring for 20 minutes; 4 U / g) to start the enzymolysis reaction, the enzymolysis temperature is 60-65°C, stirring continuously, keeping the temperature and pH of the reaction system constant, and the enzymolysis time is 240 minutes. Obtain enzymatic hydrolysis products.

[0063] 4) The enzymatic hydrolyz...

Embodiment 3

[0073] Embodiment 3, a method for enzymatically hydrolyzing mussel protein to prepare hypotensive peptides, the following steps are carried out in sequence:

[0074] 1), prepare defatted dry mussel powder:

[0075] With embodiment 1.

[0076] 2) Weigh 200.0 g of defatted dried mussel powder (protein weight content 60.23%) into the enzyme reactor, add 5 L of deionized water and stir evenly, homogenize for 10 min, and adjust the pH to 9.3 with 0.5 mol / L NaOH solution; Get a mixture.

[0077] 3), the temperature of the mixed solution was raised to 65° C., stirred and preheated, and kept under stirring for 20 minutes; 4 U / g) to start the enzymolysis reaction, the enzymolysis temperature is 65°C, the temperature and pH of the reaction system are kept constant, and the enzymolysis time is 180 minutes with continuous stirring. Obtain enzymatic hydrolysis products.

[0078] 4) The enzymatic hydrolyzate obtained in step 3) is firstly treated with enzyme inactivation to obtain an en...

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Abstract

The invention discloses a method for preparing angiotensin converting enzyme inhibitory peptide (ACEIP) by using enzymatic degradation on mussel-digested protein, which comprises the following steps: 1) mixing defatted dry mussel powder or mussel protein powder serving as raw material with water, then adjusting the pH value to 9.0 to 9.5 so as to obtain a mixed liquor; 2) raising the temperature of the mixed liquor to 60 to 65 DEG C for preheating, then adding alkali protease into the mixed liquor to carry out zymolysing reaction, wherein the zymolysing temperature is 60 to 65 DEG C and the zymolysing time is 180 to 240 minutes; and 3) carrying out enzyme inactivation processing on zymolysing products obtained in step 2 firstly so as to obtain zymolysing liquid; then sequentially carryingout ultra-filtration, freeze drying and chromatography on the obtained zymolysing liquid so as to obtain the ACEIP. The ACEIP prepared by using the method of the invention has the characteristic of strong activity.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, specifically relates to biopharmaceutical technology or functional food processing technology, in particular to a method for enzymolyzing mussel protein to prepare blood pressure-lowering peptide. Background technique [0002] Angiotensin-converting enzyme (ACE) plays an important role in the regulation of blood pressure in the human body: excessive ACE activity in the human body's circulation or in local tissues will increase the production of angiotensin II, and at the same time, the amount of bradykinin Greatly reduced, directly leading to increased blood pressure. Therefore, the effect of lowering blood pressure can be achieved by inhibiting the activity of ACE in the body. [0003] At present, ACE inhibitors have become the first-line drugs for the treatment of hypertension. Compared with synthetic ACE inhibitors, ACE inhibitory active peptides obtained by enzymatically hydrolyzing f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16
Inventor 戴志远张艳萍张婷张燕平
Owner ZHEJIANG GONGSHANG UNIVERSITY
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