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282 results about "Ace inhibitory" patented technology

ACE inhibitors are effective for control of blood pressure, congestive heart failure, and prevention of stroke and hypertension, or diabetes-related kidney damage. ACE inhibitors are especially important because they have been shown to prevent early death resulting from hypertension,...

High-potency sweetener compositon with rubisco protein, rubiscolin, rubiscolin derivatives, ace inhibitory peptides, and combinations thereof, and compositions sweetened therewith

ActiveUS20080107775A1Improve flavor profileImproving temporal profile profileNervous disorderTobacco treatmentRubiscolinAdditive ingredient
The present invention relates generally to functional sweetener compositions comprising non-caloric or low-caloric natural and / or synthetic high-potency sweeteners and methods for making and using them. In particular, the present invention relates to different functional sweetener compositions comprising at least one non-caloric or low-caloric natural and / or synthetic high potency sweetener, at least one sweet taste improving composition, and at least one functional ingredient, such as rubisco protein, rubiscolin, rubiscolin derivatives, ACE inhibitory peptide, and combinations thereof. The present invention also relates to functional sweetener compositions and methods that can improve the tastes of non-caloric or low-caloric high-potency sweeteners by imparting a more sugar-like taste or characteristic. In particular, the functional sweetener compositions and methods provide a more sugar-like temporal profile, including sweetness onset and sweetness linger, and / or a more sugar-like flavor profile.
Owner:THE COCA-COLA CO

Novel peptide with angiotonin transferase restraining liveness and method of producing the same

The present invention discloses a method of using biological enzyme decomposition to prepare a hydrolysis compound containing peptide of angiotensin transfer enzyme inhibitory activity, and three novel peptides of high ACE inhibitory activity. For the hydrolysis compound containing peptide of angiotensin transfer enzyme inhibitory activity of the present invention, the oxhide rich in praline is used as the raw material; the product of ACE inhibitory activity IC50 equal to 0.168mg / m can be prepared through the compounding and hydrolysis of biological enzyme. After ultrafiltration, the IC50 is equal to 0.079mg / ml. The means of analysis and detection determines the peptides, ISVPGPM, LGPVGNPGPA and DLSFLPQPPQQ, containing high ACE inhibitory activity. The three peptide sections are novel peptide sections containing high ACE inhibitory activity. Therefore, the three peptides have potential function of lowering blood pressure. The compounding and decomposition of biological enzyme of oxhide is a peptide type hydrolysis product, has inhibitory effects on angiotensin transfer enzyme (ACE), and can be widely used for health food, functional food, auxiliary drugs for lowering blood pressure and other products; the peptide has broad market prospects.
Owner:EAST CHINA UNIV OF SCI & TECH +1

Novel spirulina source antihypertensive peptide and preparation method thereof

The invention discloses a novel orally-effective spirulina source antihypertensive peptide and a preparation method thereof. The antihypertensive peptide is a tripeptide with the sequence of isoleucine-glutamine-proline (Ile-Gln-Pro, IQP). An angiotensin converting enzyme (ACE) inhibitory peptide is prepared by the following steps of: performing enzymolysis on spirulina platensis by adopting alkali protease, and performing ultrafiltration, gel filtration chromatography and secondary reversed phase high-performance liquid chromatography purification. Tests prove that the IQP is a noncompetitive inhibitor of ACE, and can keep good ACE inhibitory activity under digestion of main gastrointestinal protease. The IQP has good antihypertensive effect on essential hypertension rat (SHR) and has great application prospects in medicaments and functional foods for preventing and treating hypertension.
Owner:鲁军

Method for preparing wheat germ protein ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling

The invention relates to a method for preparing wheat germ protein angiotensin-converting enzyme ACE inhibitory peptide by continuous enzymolysis and ultra-filtration separation coupling; the method is carried out according to the following steps: degreasing wheat embryo powder is crashed, and the wheat germ protein is extracted by a middle-temperature amylase method; after the wheat germ protein solution is homogenized, the preparation and separation of wheat germ antihypertensive peptide is carried out by adopting the technology of protein continuous enzymolysis and the ultra-filtration separation coupling, finally, the powdered wheat germ antihypertensive peptide is obtained by compression and drying. The method can lead the product to be on-line separated so as to avoid excessive enzymolysis; meanwhile, the enzyme is utilized in a recycling way and the utilization rate of unit enzyme is improved; in addition, enzyme eliminating operation is avoided by interval operation, the continuity of the process for preparing the wheat germ protein ACE inhibitory peptide is realized. The wheat germ protein ACE inhibitory peptide which is obtained by the method can be used for preparing medicines or health care foods with the function of inhibiting the activity of the internal angiotensin-converting enzyme ACE.
Owner:JIANGSU UNIV

Method for preparing maize polypeptide through synchronous multi-frequency ultrasonic-aided enzymatic hydrolysis by immobilized enzyme

The invention relates to development and utilization of maize polypeptide products and discloses a method for preparing maize polypeptide through synchronous multi-frequency ultrasonic-aided enzymatic hydrolysis by immobilized enzyme, belonging to the technical field of deep processing of maize. The method comprises (1) a preparation process for sodium alginate-immobilized alkali protease; and (2) a process for synchronous multi-frequency ultrasonic-aided enzymatic hydrolysis of zein by immobilized enzyme. According to the invention, synchronous multi-frequency ultrasonic wave and immobilized enzyme combined technology is employed for enzymatic hydrolysis and preparation of the polypeptide product, so the method provided by the invention has the following advantages compared with conventional methods utilizing enzymatic hydrolysis, single-frequency ultrasonic-aided enzymatic hydrolysis and double-frequency ultrasonic-aided enzymatic hydrolysis: the prepared maize polypeptide has higher ACE inhibitory activity, and the yield and activity of the polypeptide are better improved. The immobilized enzyme is recoverable; the repeated utilization rate of the enzyme is higher; production cost is better saved; and a feasible approach is provided for industrial production of a high-added-value maize polypeptide product.
Owner:JIANGSU UNIV

Method for extracting fibroin peptide from mulberry silk

The invention discloses a method for extracting silk peptide from cocoon fiber, which belongs to the technical field of preparation of the silk peptide. The method comprises the following steps: the cocoon fiber is added into a Na2CO3 solution for refining, filtrated and added into a CaCl2 solution for dissolution; protease for silk dissolution solution obtained after dissolution undergoes enzymatic hydrolysis reaction; after ultrafiltraiotn and nano-filtration separation of digest, a feed liquid is obtained; and the silk peptide powder is obtained after spray drying of the feed liquid. The method has the advantages that ACE inhibitory polypeptide is enriched through ultrafiltraiotn and nano-filtration separation of the digest; the efficiency of removing CaCl2 salt is high; the method has the advantages of large processing capacity, mild separation condition, simple flow, low energy consumption and so on; and the industrial production is easy to realize.
Owner:TIANJIN UNIV

Preparation method of rice bran protein peptide with ACE inhibitory activity

The invention discloses a preparation method of rice bran protein peptide with ACE inhibitory activity. The method is characterized in that rice bran is adopted as a raw material, and comprises the following steps: firstly extracting rice bran protein, then preparing rice bran protein hydrolysate by adopting an enzyme hydrolysis method, and separating and purifying the rice bran ACE inhibitory peptide by adopting a hyperfiltration method, sephadex G-15 and a RP-HPLC method. According to the preparation method, the rich and cheap rice bran resource is effectively utilized to prepare the rice bran protein peptide with the high ACE inhibitory activity. The decompression function of the rice bran ACE inhibitory peptide can be maximally utilized, so that on one hand, the additional value of the rice bran is increased, and the good economic benefit and market value can be created; on the other hand, the morbidity of the hypertension can be effectively reduced, the high morbidity can be controlled, the human beings can be protected from the harm of the hypertension, the application value and social benefit are good, and the theoretical foundation and the experiment evidence can be provided for researching the structure and decompression mechanism of the rice bran protein ACE inhibitory peptide.
Owner:黑龙江省北大荒米业集团有限公司

Preparation of dairy ACE inhibitory peptide

Milk ACE inhibiting peptide is prepared by: hydrolyzing milk casein as raw material with protease, heating the hydrolytic liquid to inactivate protease, centrifugally separating to obtain supernatant, desalting with ion exchange resin, freezing to dry and obtain high active edible ACE inhibiting peptide. The product can be used as food additive as functional factor or as food supplementary to add into various foods.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity

The invention relates to a lactobacillus and in particular relates to a low-temperature resistant lactobacillus with ACE (angiotensin converting enzyme) inhibitory activity. The low-temperature resistant lactobacillus with the ACE inhibitory activity is lactobacillus plantarum I1 which is preserved in the China general microbiological culture collection center of which the preservation address is No.3, No1. yard, Beichen west road, Chaoyang District of Beijing, the preservation data is September 18th, 2012, and the preservation number is CGMCC No.6575; the lactobacillus plantarum I1 has the ACE inhibitory rate being 66%, can tolerate 6% of NaCl and 100mg / kg of nitrite, and can fast generate acid; the growing temperature of the lactobacillus plantarum I1 is 4-40 DEG C, and is suitable for low-temperature fermentation; and the low-temperature resistant lactobacillus has obvious protease activity and lipase activity, can not generate mucus, has an obvious antibacterial effect on staphylococcus aureus and colon bacillus, and can be used as a meat product leavening agent strain under a low-temperature condition.
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY

Walnut peptide having ACE inhibitory activity and preparation method thereof

The invention discloses walnut peptide having the ACE inhibitory activity and a preparation method thereof. The amino acid sequence of the walnut peptide is EPNGLLLPGY, and the preparation method of the walnut peptide comprises the steps of taking walnut pomace as raw materials, selecting appropriate protease, preparing walnut antihypertensive peptide coarse products according to the optimal enzymolysis technology, adopting the coupling chromatographic technique of the membrane technology for conducting fine preparation on the walnut antihypertensive peptide, adopting the high performance liquid chromatography for conducting further purification, and adopting the vacuum freeze drying or low-temperature spray drying technology for preparing final products. According to the walnut peptide, the walnut pomace is adopted as the raw materials for developing the protein peptide, the raw materials are abundant, the price is low, and the large-scale industrialized production can be satisfied; the walnut peptide has important significance in walnut protein product diversification and development and utlization of walnut biological active peptide in functional foodstuff, health food or medicine.
Owner:HARBIN INST OF TECH

Method for preparing ACE inhibitory peptides through bean pulp enzymolysis

The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through bean pulp enzymolysis. The method comprises the following steps that (1) high-temperature bean pulp is crushed and sieved, obtained bean pulp powder and water are mixed according to a mass ratio of 1:(4-6), the pH value is regulated to 8 to 10, and a bean pulp mixture is obtained; (2) the pH value of the bean pulp mixture is regulated to 7 to 9, a complex enzyme preparation is added, the mixture is uniformly mixed, hydrolysis is carried out for 5 to 8 hours at the temperature being 35 to 50 DEG C, after the hydrolysis is completed, hydrolysis liquid is boiled in a boiling water bath for enzyme deactivation, and mixed liquid is obtained; (3) the pH value of the mixed liquid is regulated to 6.5 to 7.5, supernatant is taken, ultrafiltration treatment is performed through an ultrafiltration membrane, and filtered liquid is obtained; and (4) the filtered liquid is subjected to vacuum freezing drying, and the ACE inhibitory peptide powder is obtained. The method for preparing ACE inhibitory peptides through bean pulp enzymolysis has the advantages that the process is simple and is easy to implement; in the whole process, the reaction conditions are mild; the compounded complex enzyme preparation is used; and the industrial processing operation is convenient.
Owner:TIANJIN MODERN VOCATIONAL TECH COLLEGE

A mung bean protein peptide having ACE inhibitory activity and a preparation method and applications thereof

The present invention discloses a mung bean protein peptide having ACE inhibitory activity and a preparation method and applications thereof. According to the invention, the preparation method comprises: mixing water and mung bean protein powder to prepare mung bean protein slurry, after the pH of the slurry is adjusted to 3.5-5, adding cellulase for hydrolysis at 45-55 DEG C, after the enzyme hydrolysis, deactivating the cellulose, centrifugating, and washing with water to remove impurities; and then adjusting the pH to be 8-9, adding alkaline protease for hydrolysis at 50-60 DEG C, after the enzyme hydrolysis, deactivating the alkaline protease and obtaining the mung bean protein peptide having ACE inhibitory activity after coarse filtration, fine filtration and nanofiltration, and drying. According to the invention, the preparation method is energy efficient and convenient in operation, product quality and production efficiency are improved, and the obtained protein peptide has the ACE inhibitory activity of 42.8-59.6[mu]g / mL in terms of IC50, and can be widely used as a health food, or materials general food, in particular for the preparation of antihypertensive products assist.
Owner:广州合诚实业有限公司

Ace inhibitory peptides from plant materials

Improved processes are provided for preparing ACE inhibitory peptide containing hydrolysates from a plant material such as a seed meal or flour. In one embodiment, the seed meal or flour is extracted with an organic solvent prior to digestion. Also provided are ACE inhibitory peptides Val-Ser-Val and Phe-Leu.
Owner:AGRI & AGRI FOOD

ACE inhibitory peptide and preparation method thereof

InactiveCN103275176AWith ACE inhibition functionHas antihypertensive propertiesMilk preparationPeptidesAnti hypertensionAmino acid
The invention discloses an ACE inhibitory peptide and a preparation method thereof, and provides a casein hydrolysate which has an anti-hypertension effect and is rich in the ACE inhibitory peptide, the ACE inhibitory peptide with an anti-hypertension effect, and a preparation method of the ACE inhibitory peptide. The casein hydrolysate rich in the ACE inhibitory peptide contains two peptide fragments with ACE inhibitory activities; and the amino acid sequences of the two peptide fragments with ACE inhibitory activities are respectively YS and YQKFPQYLQY. The amino acid sequence of the ACE inhibitory peptide is YS or YQKFPQYLQY. The ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is sufficiently mixed with milk powder matrixes to prepare anti-hypertension milk powder; or the ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is mixed with fresh milk to obtain a mixture, and lactic acid bacteria is inoculated in the mixture to obtain yoghourt with an anti-hypertension effect. The dairy product has a remarkable anti-hypertension effect, has no influence on the normal blood pressure, is safe to eat, and is suitable for being ate by the hypertension groups.
Owner:TIANJIN UNIV OF COMMERCE

Two kinds of ACE inhibitory peptides derived from Larimichthys crocea connetin

InactiveCN108033995AGood ACE inhibitory activity in vitroStrong inhibitory activityTripeptide ingredientsPeptidesTitinBlood pressure
The invention mainly relates to Larimichthys crocea connetin source ACE inhibitory peptide sequences and applications thereof. The two kinds of peptide sequences respectively are WAR and WQR, and establish a basis for prevention and treatment of hypertension. Different on-line databases are used, and after multiple times of screening, the inhibitory peptides with high efficient ACE inhibition activity are obtained. WAR and WQR are artificially synthesized, in order to verify external ACE inhibition activity of the peptides by using a high performance liquid chromatography method. The inventionrelates to two kinds of active peptides with good external ACE inhibition activity. The two kinds of peptide sequences are used for developing health food with blood pressure adjusting effects, and the purpose for prevention, control, alleviation and auxiliary treatment of hypertension is realized by long-term usage.
Owner:BOHAI UNIV

Green tea goat milk with function of reducing blood pressure and preparation method of green tea goat milk

The invention provides green tea goat milk with a function of reducing blood pressure and a preparation method of the green tea goat milk. Goat milk beverage which is rich in ACE inhibitory peptides is prepared by compound enzymolysis, is low in production cost, not limited by resource and small in environmental pollution, and can be mass-produced, and products are safe. On the basis, ultra-fine green tea powder is prepared by enzymic hydrolysis of cell walls and a low-temperature ultra-fine pulverizing technology, specific surface area is increased, and a large number of effective constituents are released. The ultra-fine green tea powder is added in the goat milk in proportion, and the obtained product not only has various nutrient substances and tea fragrance in green tea, but also has treatment effect of reducing high blood pressure, and has wide application prospect in the food industry. The tea powder is prepared by low-temperature ultra-fine pulverization, the original color, flavor and taste quality of tea leaves can be maintained effectively, after the tea leaves is pulverized into ultra-fine powder, the specific surface area of the tea powder is increased obviously, the effective constituents are released easily, human absorption can be improved, and therefore, nutrition absorptivity of human to the tea leaves is improved. Ultra-fine pulverization is a pure physical process, and pure natural property of processed products is maintained.
Owner:SHAANXI NORMAL UNIV +1

Method for preparing silkworm chrysalis source ACE (Angiotensin Converting Enzyme) inhibitory peptide by ultrasonic-ionic liquid coupling technology

The invention discloses a method for preparing a silkworm chrysalis source ACE (Angiotensin Converting Enzyme) inhibitory peptide from by an ultrasonic-ionic liquid coupling technology. The method comprises the following steps: preparing silkworm chrysalis protein into a suspension by using distilled water, subsequently mixing the suspension with an isopyknic ionic liquid and adding into a flask, dipping an ultrasonic probe into a water phase so as to perform ultrasonic-ionic liquid coupling treatment, after the treatment is accomplished, centrifuging, collecting the silkworm chrysalis protein and recycling the ionic liquid, firstly washing the collected silkworm chrysalis protein and subsequently centrifuging so as to remove the residual ionic liquid, and preparing the ACE inhibitory peptide from pretreated silkworm chrysalis protein by using an enzyme method. Compared with the conventional untreated method, the method for preparing the silkworm chrysalis source ACE inhibitory peptide by an ultrasonic-ionic liquid coupling technology remarkably improves the activity of the ACE inhibitory peptide, that is, the activity is increased by 34-54%.
Owner:JIANGSU UNIV OF SCI & TECH

Method for preparing angiotensin converting enzyme (ACE) inhibitory peptide from sea cucumber

ActiveCN102517364AACE inhibitory activity is potentStructurally intactFermentationAngiotensin-converting enzymeMicroorganism
The invention relates to a method for preparing an angiotensin converting enzyme (ACE) inhibitory peptide from sea cucumber. The method comprises the following steps of: de-salting and softening raw materials including fresh sea cucumber, or dry sea cucumber, or soaked sea cucumber, or high-pressure sea cucumber; after preparing slurry, adding water into the slurry; heating the slurry to kill microorganisms; after cooling the slurry, adding composite proteolytic enzyme or flavor proteolytic enzyme into the slurry to perform enzymolysis; killing enzymes; after cooling, centrifuging the product to remove undissolved substances; filtering the supernatant fluid in a pipe; filtering the filtrate with a 3,000 Dalton organic membrane to trap and classify; and performing nano filtration and drying on the trapped liquid which is less than 3,000 Dalton in mass to obtain the ACE inhibitory peptide. A zymolytic technical scheme is optimized; and due to the separation and extraction technology of pipe filtration, ultrafiltration and nano filtration, the extraction efficiency is high, and the pressure-reducing effect of the obtained ACE inhibitory peptide is good. The method is reasonable in process, simple and convenient in operation and high in operability; and industrial production is easy to realize.
Owner:山东海普盾生物科技有限公司

Method for preparing egg white source ACE inhibitory peptide microcapsules

The invention belongs to the field of deep processing of edible protein and comprehensive utilization of by-products thereof and discloses a method for preparing egg white source ACE inhibitory peptide microcapsules. The method is characterized by comprising the following steps: with egg white protein powder with protein content of 80-90% as a raw material, carrying out enzymolysis, ultrafiltration, vacuum freeze drying and microcapsule embedding process of spray condensation technology to prepare the egg white source ACE inhibitory peptide microcapsules. The embedding rate and ACE inhibition ratio of a microcapsule product obtained according to the preparation method provided by the invention are higher than 80%, the microcapsule product is natural and safe and is good in palatability and stability, and moreover, the peptide digestion is not affected. Therefore, the technology for preparing the egg white source ACE inhibitory peptide microcapsules provided by the invention provides an efficient and new way for deep processing and comprehensive utilization of egg products.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY

Method for preparing angiotensin I-converting enzyme inhibitory peptide by using squid liver protein

The invention relates to a method for preparing angiotensin I-converting enzyme (ACE) inhibitory peptide by using a squid liver protein, which comprises: treating squid liver protein from which fish oil is extracted with acetone to degrease; hydrolyzing the squid liver protein in the presence of pepsase for 18 to 25 hours under conditions that: the hydrolysis temperature is 35 to 38 DEG C, the pH is 1.5 to 2.5, the ratio of the pepsase to a substrate is 2 to 3 percent, and the concentration of the substrate is 1 to 3 percent; and subjecting enzymolysis solution to ultrafiltration treatment, tracking the ACE inhibiting activity of the ACE inhibitory peptide, and separating and purifying the ACE inhibitory peptide by using gel filtration chromatography, diethylaminoethanol (DEAE) anion exchange, reversed-phase-high performance liquid chromatography (RP-HPLC) and other methods. The ACE inhibiting activity of the prepared ACE inhibitory peptide is basically unchanged at a temperature of 2 to 100 DEG C and a pH value of 1 to 12 and is stable; and in a simulation gastrointestinal digestion experiment, the ACE inhibiting activity of the components is not affected, and the peptide still has high ACE inhibiting activity after being digested in gastrointestinal tract. The method is reasonable in process, simple in operation, reduces waste discharge, changes waste materials into valuable materials, and the prepared ACE inhibitory peptide can be used in the field of development of blood pressure reducing foods and medicines.
Owner:ZHEJIANG OCEAN UNIV

ACE inhibitory peptide and preparation method and application

The invention discloses an ACE inhibitory peptide and a preparation method and application. The amino acid sequence of the ACE inhibitory peptide is GPPGP and is derived from natural animal proteins,the IC50 of the ACE inhibitory peptide is 190.43 micromole per liter, the molecular weight is small, and the ACE inhibitory peptide has the advantages of being stable, safe, easy to absorb by the human body and the like and can be used for industrial production. The ACE inhibitory peptide with the amino acid sequence of GPPGP can be used for preparing hypertension treatment / prevention medicine orserving as a functional food additive for long-term treatment and health care of hypertension patients.
Owner:JIMEI UNIV

ACE (angiotension converting enzyme) inhibitory peptide and preparation method thereof

InactiveCN104592350AWith ACE inhibition functionHas antihypertensive propertiesMilk preparationPeptidesLactobacillusAnti hypertension
The invention discloses an ACE (angiotension converting enzyme) inhibitory peptide and a preparation method thereof, and provides an ACE inhibitory peptide with an anti-hypertension effect and a preparation method of the ACE inhibitory peptide. An amino acid sequence of the ACE inhibitory peptide is YS. The preparation method comprises the following steps: sufficiently mixing the ACE inhibitory peptide with a milk powder substrate to prepare anti-hypertension milk powder; or mixing the ACE inhibitory peptide with fresh milk, and inoculating lactic acid bacteria to ferment to obtain yoghourt with the anti-hypertension effect. The milk product has a remarkable antihypertensive effect, is free of interferences on normal blood pressure, safe to eat, and suitable for the hypertension crowds.
Owner:TIANJIN UNIV OF COMMERCE

Puffer fish bioactive peptide and preparation method thereof

The invention discloses a puffer fish bioactive peptide and a preparation method thereof and belongs to the technical field of ACE (Angiotensin Converting Enzyme) inhibitory peptides. The preparationmethod of a puffer fish bioactive peptide comprises the following steps: S1, preparation of a puffer fish enzymatic hydrolysate; S2, ultrafiltration separation of the puffer fish enzymatic hydrolysate; S3, separation and purification of polypeptide; S4, sequence identification of polypeptide; and S5, polypeptide synthesis and activity determination. According to the puffer fish bioactive peptide,the bioactive peptide is octapeptide, and the amino acid sequence of the puffer fish bioactive peptide is PPLLFAAL (Pro-Pro-Leu-Leu-Phe-Ala-Ala-Leu). According to the puffer fish bioactive peptide andthe preparation method thereof, the puffer fish skin is adopted as a raw material, an octapeptide amino acid sequence is obtained, and the prepared polypeptide has the ACE inhibitory activity.
Owner:FISHERIES RES INST OF FUJIAN

Mung bean yoghourt product having function of reducing blood pressure and preparation method thereof

The invention provides a mung bean yoghourt product having the function of reducing the blood pressure and a preparation method thereof. The mung bean yoghourt product is prepared from mung bean extract and milk powder, wherein the mung bean extract is prepared by soaking mung beans in a glutamic acid solution; and the mung bean extract accounts for 80 to 90 weight percent, and the milk powder accounts for 10 to 20 weight percent. The preparation method comprises: soaking the mung beans in the glutamic acid solution, accelerating germination of the mung beans, and performing seed coat removal,homogenizing and high-temperature amylase treatment to obtain the mung bean extract; adding skim milk powder into the mung bean extract, performing mixing and homogenization treatment, adding protease for enzymolysis, and obtaining a fermentation substrate; and performing enzyme killing, sterilization, cooling and lactobacillus inoculation on the fermentation substrate, and performing fermentation on the fermentation substrate to prepare the mung bean yoghourt product. The mung bean yoghourt product not only is rich in GABA but also contains ACE inhibitory peptide, has good effect of reducingthe blood pressure, not only can be directly used for preparing related health food but also can be used as a functional composition and added into other health products, and has wide application prospect.
Owner:瑞康东方医学研究(北京)有限公司

Method for preparing indissoluble aquatic collagen protein source ACE inhibitor peptides

The invention discloses a preparation method of a less soluble aquatic collagen source ACE inhibitory peptide comprising: cutting and crushing fresh marine organism at low temperature, adding a caustic soda to stir at low temperature, removing a supernatant, cleaning precipitates and adding an organic acid solution and an acid protease, stirring at low temperature, obtaining the high molecule collagen and less soluble collagen through speed centrifugation, enabling the less soluble collagen denaturation at high temperature and high pressure, forming a soluble collagen solution, obtaining soluble collagen powder; adding a sodium hydrate solution and the protease to stir at optimal temperature, obtaining an enzymolysis protein peptide solution, centrifugally separating said solution, collecting the supernatant, preparing the less soluble aquatic collagen source ACE inhibitory peptide after the freeze drying. The method of the invention fully use the dregs after the high molecule collagen extraction, obtains the hot water soluble collagen, and obtains the collagen peptide through enzymolysis, prepares eatable ACE inhibitory peptide products and improves the high molecule collagen productivity.
Owner:HAINAN UNIVERSITY

Method for preparing gluten ACE inhibitory peptide through multi-mode ultrasonic wave strengthened enzymolysis

The present invention provides a method for preparing a gluten ACE inhibitory peptide through multi-mode ultrasonic wave strengthened enzymolysis, and relates to the technical field of deep processing of agricultural products. According to the method, an ultrasonic pretreatment is applied on a gluten suspension, wherein the sequential work mode frequency combination is 20-35 kHz, the sequential time is 5 s and 5 s, the synchronous work mode frequency combination is 20-28 kHz, the pulse time is 10 s, the interval time is 5 s, the ultrasonic power per unit volume is 200 W / L, and the pretreatment time is 15 min; alkaline protease is added to carry out enzymolysis after the pretreatment, wherein the enzymolysis time is 30 min; and the enzyme is inactivated for 10 min through a 100 DEG C boiling water bath, centrifugation is performed for 10 min under 10000 g, and the supernatant is collected so as to obtain the extraction liquid of the gluten ACE inhibitory peptide. According to the present invention, the gluten suspension is subjected to the multi-mode ultrasonic wave pretreatment so as to increase the enzymolysis efficiency, wherein the ACE inhibition rate activity of the hydrolysate is increased by 16.4-25.4% compared with the traditional enzymolysis method, and the ACE inhibition rate activity of the hydrolysate is increased by 6.0-13.7% compared with the counterflow type single-frequency ultrasound.
Owner:JIANGSU UNIV

Method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through compound enzymatic hydrolysis of feta protein

The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through compound enzymatic hydrolysis of feta protein. The method comprises the following steps of: centrifuging and degreasing recovered goat milk taken as a raw material, preparing casein by an isoelectric precipitation method, carrying out compound hydrolysis on the casein through neutral protease and trypsase under the conditions of specific temperature and pH, optimizing the hydrolysis condition, treating the hydrolytic process with ultrasound waves to obtain small-molecule ACE inhibitory peptides and determining the activity of the small-molecule ACE inhibitory peptides. The inhibitory proportion of an ACE inhibitory peptide product prepared by the method can reach above 80%; the ACE inhibitory peptide product has small molecular mass, can be absorbed by a human body in the form of complete peptide fragments and cannot cause the immunological rejection of the human body; and the ACE inhibitory peptide product has an obvious antihypertensive effect on hypertensive, has no effect on people with normal blood pressure and has the characteristics of little dosage, no toxic or side effect, mild property and strong physiological function.
Owner:SHAANXI UNIV OF SCI & TECH

Technological method for co-production of ACE inhibitory peptides and immunological competence peptides of mung beans with enzymic method

The invention relates to a technological method for co-production of ACE inhibitory peptides and immunological competence peptides of mung beans with an enzymic method. The technological method for co-production of the ACE inhibitory peptides and the immunological competence peptides of the mung beans with the enzymic method comprises the steps that mung bean albumen powder is dissolved in micromolecule water and is prepared into an albumen solution with the concentration, by mass, ranging from 7% to 40%, enzymolysis and inactivation are conducted on mung bean albumen, and supernate is moved out; mung bean antihypertensive peptides are prepared, wherein mung bean peptides with the molecular weight of 1 kDa and 3 kDa respectively are processed through an ultrafiltration membrane, and an ACE inhibitory peptide powder product of the mung beans is obtained by conducting freezing drying on the 1 kDa-3 kDa filtered solution obtained after interception is conducted; the immunological competence peptides of the mung beans are prepared, wherein the residual solution is hydrolyzed continually for 2-3 hours through neutral protease and flavourzyme, and supernate is moved out; a 1 kDa ultrafiltration membrane is used for intercepting the mung bean peptides with the molecular cut off smaller than 1 kDa in the prepared supernate, desalination is conducted through the ultrafiltration membrane, and the immunological competence peptides of the mung beans can be obtained by conducting freezing drying on the processed filtrate. The mung bean peptides having different biological functions are prepared through one set of technical conditions, the process is simple and efficient, and the resource utilization rate is high.
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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