Method for preparing ACE inhibitory peptides through bean pulp enzymolysis

Abstract

The invention provides a method for preparing ACE (Angiotensin Converting Enzyme) inhibitory peptides through bean pulp enzymolysis. The method comprises the following steps that (1) high-temperature bean pulp is crushed and sieved, obtained bean pulp powder and water are mixed according to a mass ratio of 1:(4-6), the pH value is regulated to 8 to 10, and a bean pulp mixture is obtained; (2) the pH value of the bean pulp mixture is regulated to 7 to 9, a complex enzyme preparation is added, the mixture is uniformly mixed, hydrolysis is carried out for 5 to 8 hours at the temperature being 35 to 50 DEG C, after the hydrolysis is completed, hydrolysis liquid is boiled in a boiling water bath for enzyme deactivation, and mixed liquid is obtained; (3) the pH value of the mixed liquid is regulated to 6.5 to 7.5, supernatant is taken, ultrafiltration treatment is performed through an ultrafiltration membrane, and filtered liquid is obtained; and (4) the filtered liquid is subjected to vacuum freezing drying, and the ACE inhibitory peptide powder is obtained. The method for preparing ACE inhibitory peptides through bean pulp enzymolysis has the advantages that the process is simple and is easy to implement; in the whole process, the reaction conditions are mild; the compounded complex enzyme preparation is used; and the industrial processing operation is convenient.

Description

technical field [0001] The invention belongs to the technical field of food, and in particular relates to a method for preparing ACE inhibitory peptides by enzymatically hydrolyzing soybean meal. Background technique [0002] ACE inhibitory peptides, namely angiotensin-converting enzyme inhibitory peptides (ACEIP, AngiotensinConvertingEnzymeInhibitoryPeptides), are some polypeptide substances that inhibit angiotensin-converting enzyme (ACE) activity. The amino acid sequences and peptide chain lengths of these polypeptides are different, but all It has a similar function, that is, it has the function of lowering blood pressure, so it is also called blood pressure lowering peptide. In 1981, the first synthetic orally effective peptide ACE inhibitor, captopril, came out. So far, many chemically synthesized ACE inhibitors have been used clinically. However, the action time of this kind of drugs is not long, and blood pressure will rebound after drug withdrawal; excreted by the ...

AI Technical Summary

Problems solved by technology

[0004] At present, ACE inhibitory peptides have not been put into large-scale production and application. The main reasons are: the lack of cheap, effective and easy-to-manufacture enzyme preparations, the inability to effectively control the molecular weight of enzymatic hydrolysis products, which seriously affects the purification effect in the later stage; the inability to effectively solve the problem of enzymatic hydrolysis The bitterness of the product limits its application in the food field