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Method for preparing angiotensin I-converting enzyme inhibitory peptide by using squid liver protein

A technology of angiotensin and fish liver, which is applied in the field of deep processing of squid liver protein, can solve the problems of increased difficulty in separation and extraction, large structural differences, and complex components of proteolysis products, and achieves easy operation, easy control operation, and high ACE inhibitory activity Effect

Inactive Publication Date: 2011-11-23
ZHEJIANG OCEAN UNIV
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  • Abstract
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Problems solved by technology

[0006] Since the amino acid composition and structure of ACE inhibitory peptides are quite different, there is no fixed or special amino acid composition, and the composition of proteolysis products is quite complex, which increases the difficulty of its separation and extraction.

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  • Method for preparing angiotensin I-converting enzyme inhibitory peptide by using squid liver protein
  • Method for preparing angiotensin I-converting enzyme inhibitory peptide by using squid liver protein
  • Method for preparing angiotensin I-converting enzyme inhibitory peptide by using squid liver protein

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Embodiment Construction

[0035] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.

[0036]A method utilizing squid liver protein to prepare angiotensin-converting enzyme (ACE) inhibitory peptides, specifically comprising the following steps:

[0037] 1. Extract squid liver protein by acetone extraction method:

[0038] Add water at a ratio of 1:1.5, fill with nitrogen for 10 minutes, and add L-ascorbic acid-6-palmitate accounting for 0.2% of the oil to prevent oxidation. Centrifuge at 8000rpm / min for 30min, the uppermost layer is the fish oil layer, after centrifuging the fish oil, wash with acetone several times, quickly dehydrate the tissue to make acetone powder, and vacuum dry to obtain the defatted squid liver protein;

[0039] 2. Use pepsin to enzymatically hydrolyze squid liver protein: hydrolyze squid liver protein for 20 hours at a hydrolysis temperature of 36°C, a pH of 1.5-2.5, an enzyme-to-substrate ratio of 2%, ...

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Abstract

The invention relates to a method for preparing angiotensin I-converting enzyme (ACE) inhibitory peptide by using a squid liver protein, which comprises: treating squid liver protein from which fish oil is extracted with acetone to degrease; hydrolyzing the squid liver protein in the presence of pepsase for 18 to 25 hours under conditions that: the hydrolysis temperature is 35 to 38 DEG C, the pH is 1.5 to 2.5, the ratio of the pepsase to a substrate is 2 to 3 percent, and the concentration of the substrate is 1 to 3 percent; and subjecting enzymolysis solution to ultrafiltration treatment, tracking the ACE inhibiting activity of the ACE inhibitory peptide, and separating and purifying the ACE inhibitory peptide by using gel filtration chromatography, diethylaminoethanol (DEAE) anion exchange, reversed-phase-high performance liquid chromatography (RP-HPLC) and other methods. The ACE inhibiting activity of the prepared ACE inhibitory peptide is basically unchanged at a temperature of 2 to 100 DEG C and a pH value of 1 to 12 and is stable; and in a simulation gastrointestinal digestion experiment, the ACE inhibiting activity of the components is not affected, and the peptide still has high ACE inhibiting activity after being digested in gastrointestinal tract. The method is reasonable in process, simple in operation, reduces waste discharge, changes waste materials into valuable materials, and the prepared ACE inhibitory peptide can be used in the field of development of blood pressure reducing foods and medicines.

Description

technical field [0001] The invention relates to a method for deep processing squid liver protein, in particular to a method for preparing angiotensin-converting enzyme (ACE) inhibitory peptide by using squid liver protein. Background technique [0002] During squid processing, wastes such as head, feet, liver and epidermis, which account for 30% of the squid's weight, are generated. For these wastes, the general treatment method is to process fishmeal or bury them, which is not only a huge waste of fishery resources, but also has the problem of environmental pollution. In foreign countries, such as Spain and Japan, the utilization of squid liver adopts its own enzymatic fermentation method to produce squid soluble slurry, squid meal, etc. as fish feed. The utilization and research of squid liver in China still has relatively large development space. At present, there is only the processing of dried squid liver powder, and it is used as feed, and the technical level is not h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/435
Inventor 谢超霍建聪丁璞
Owner ZHEJIANG OCEAN UNIV
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