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Novel spirulina source antihypertensive peptide and preparation method thereof

A blood pressure-lowering peptide and spirulina technology, which is applied in the preparation and purification of new-type spirulina-sourced blood-pressure-lowering peptides, can solve the problems of poor absorption of macromolecular proteins, low activity and purity, and unclear types of active peptides

Inactive Publication Date: 2010-12-08
鲁军
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the disadvantages of poor absorption of macromolecular proteins and the current active peptide varieties are mixed, activity and purity are not high, and obtain a spirulina source blood pressure lowering peptide with a high blood pressure lowering effect in the body

Method used

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  • Novel spirulina source antihypertensive peptide and preparation method thereof
  • Novel spirulina source antihypertensive peptide and preparation method thereof
  • Novel spirulina source antihypertensive peptide and preparation method thereof

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Embodiment Construction

[0017] The present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments, but the scope of the present invention is not limited. Its specific process route consists of the following steps:

[0018] 1. Preparation of spirulina protein enzymatic hydrolyzate

[0019] Suspend 20g of spirulina powder in 160mL of Milli-Q water, and freeze-thaw the suspension five times in a liquid nitrogen-water bath at 25-30°C. Ultrasonic crushing (200W, amplitude 60%) for 3min, centrifugation at a rate of 6000g for 30min at 4°C, and removal of precipitates after centrifugation to obtain protein supernatant, except for a part of which was determined for protein content by Bio-Rad Protein Assay, the remaining The supernatant was quickly freeze-dried in vacuo and stored at -20°C until use.

[0020] Spirulina protein solution with a substrate concentration of 2% (equivalent to 20 mg / mL), adjust the pH value to 8.5, add Alcalase2.4L...

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Abstract

The invention discloses a novel orally-effective spirulina source antihypertensive peptide and a preparation method thereof. The antihypertensive peptide is a tripeptide with the sequence of isoleucine-glutamine-proline (Ile-Gln-Pro, IQP). An angiotensin converting enzyme (ACE) inhibitory peptide is prepared by the following steps of: performing enzymolysis on spirulina platensis by adopting alkali protease, and performing ultrafiltration, gel filtration chromatography and secondary reversed phase high-performance liquid chromatography purification. Tests prove that the IQP is a noncompetitive inhibitor of ACE, and can keep good ACE inhibitory activity under digestion of main gastrointestinal protease. The IQP has good antihypertensive effect on essential hypertension rat (SHR) and has great application prospects in medicaments and functional foods for preventing and treating hypertension.

Description

technical field [0001] The invention relates to a preparation and purification method of a novel spirulina-derived blood pressure-lowering peptide, which can be applied to nutritional products, health-care functional foods and medicines, and belongs to the field of food biotechnology. Background technique [0002] Bioactive peptides refer to peptide compounds that are beneficial to the life activities of organisms or have physiological effects. After being hydrolyzed by various enzymes in the digestive tract, the protein ingested by humans is not only absorbed in the form of amino acids, but more directly absorbed in the form of low peptides, and the absorption speed of dipeptides and tripeptides is faster than that of amino acids of the same composition. quick. Therefore, the biological significance of bioactive peptides is mainly reflected in two aspects: its absorption mechanism is better than that of amino acids and it has incomparable physiological functions of amino a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/083C07K1/20C07K1/16C12P21/06A23L1/29A23L1/305A61K38/06A61P9/12A23L33/18
Inventor 鲁军任迪峰田之倉優王建中
Owner 鲁军
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