The invention discloses a probiotics microcapsule wall material preparation method. The probiotics microcapsule wall material preparation method comprises the following steps: mixing sodium alginate,carrageenan and meal fiber, performing ultraviolet lamp irradiation for 15-30 min, mixing a mixture with an aseptic phosphoric acid buffer solution with a pH value being with 6.8-7.4, performing water-bath dissolving at the temperature of 70-85 DEG C to prepare a wall material solution with mass concentration being 8.5-10.5%, wherein in the wall material solution, the mass concentration of the meal fiber is 1-5%, the mass concentration of sodium alginate is 2-4%, and the mass concentration of carrageenan is 4.5-6.5%. The invention also discloses the preparation method of the probiotics microcapsule, the volume ratio of an oligosaccharide solution (the mass concentration is 3-5%) to a probiotics suspension (greater than 5*107 CFU / mL) is 1:2-2:1. The probiotics microcapsule has the advantages of high embedding rate, high live bacteria number, high stress resistance, simple operation, economic performance, easy operation, and is suitable for industrial production.