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Novel thermophilic lactobacillus and application thererof in wet corn soaking process

A thermophilic lactic acid bacteria, bacteria mud technology, applied in the directions of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of short shelf life, difficult transportation and low stability of lactic acid bacteria liquid, and achieve enhanced freeze-drying. The effect of survival rate, efficiency improvement, and yield improvement

Active Publication Date: 2019-01-18
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the lactic acid bacteria liquid has limitations such as short storage period, low stability and difficult transportation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The screening of embodiment 1LAB 0#, LAB 1#

[0034] Take 10mL of corn soaking water (old pulp) and dilute to 10% of the original concentration respectively.-1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 and 10 -7 times, and then take 0.3mL respectively spread on the MC plate, (50 ± 2) ℃ culture 24h. Pick a single colony with a calcium-dissolving circle, streak it on a plate, separate it, and purify it repeatedly. The colonies with large and unique calcium-dissolving circles were isolated and stored on the slant, and they were numbered LAB0# and LAB 1# respectively. The corn soaking water (old pulp) is provided by the corn starch production workshop of Linghua Group Co., Ltd. (Jining, Shandong).

[0035] MC medium formula: soybean peptone 5.0g / L, beef extract powder 3.0g / L, yeast extract powder 3.0g / L, glucose 20.0g / L, lactose 20.0g / L, calcium carbonate 10.0g / L, agar 15.0g / L, neutral red 0.05g / L, pH value at 25°C is 6.0±0.1.

Embodiment 2

[0036] The preparation of embodiment 2 microbial inoculum A

[0037] (1) Preparation of seed liquid: insert thermophilic lactic acid bacteria LAB 0# into MRS liquid medium, and cultivate seed liquid for 24 hours at 50-55°C on a shaker at 100r / min; the formula of MRS liquid medium is One liter of MRS liquid medium contains peptone 10.0g, beef extract 5.0g, yeast extract 4.0g, glucose 20.0g, Tween-80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, triammonium citrate 2.0g , magnesium sulfate 0.2g, manganese sulfate 0.05g, and the balance is distilled water;

[0038] (2) Preparation of bacterium slime: the seed liquid that step (1) obtains is inserted in the fermentation medium by the inoculum size of 5%, after 100r / min shaker culture at 50-55 ℃ for 24 hours, the fermented product is centrifuged ( Centrifugal speed is 5000r / min, centrifugation time 10min), discard the supernatant, collect the thallus, wash with normal saline to obtain the bacterium sludge; where...

Embodiment 3

[0042] The preparation of embodiment 3 microbial inoculum A

[0043] (1) Preparation of seed liquid: insert thermophilic lactic acid bacteria LAB 0# into MRS liquid medium, and cultivate seed liquid for 24 hours at 50-55°C on a shaker at 120r / min; the formula of MRS liquid medium is One liter of MRS liquid medium contains peptone 10.0g, beef extract 5.0g, yeast extract 4.0g, glucose 20.0g, Tween-80 1.0mL, dipotassium hydrogen phosphate 2.0g, sodium acetate 5.0g, triammonium citrate 2.0g , magnesium sulfate 0.2g, manganese sulfate 0.05g, and the balance is distilled water;

[0044] (2) Preparation of bacterium slime: the seed liquid that step (1) obtains is inserted in the fermentation medium by the inoculum size of 5%, after 120r / min shaker culture at 50-55 ℃ for 24 hours, the fermented product is centrifuged ( Centrifugal speed is 5000r / min, centrifugation time 15min), discard supernatant, collect thalli, wash with normal saline, promptly obtain bacterium slime; Wherein the ...

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Abstract

The invention relates to a novel thermophilic lactobacillus and the application thererof in the wet corn soaking process. The bacterial species preservation information of the thermophilic lactobacillus is as follows: name of preservation unit: Common Microbial Center of China Microbial Strain Collection Management Committee; Depository address: Institute of Microbiology, Chinese Academy of Sciences, No. 3 Academy, No. 1 Beichen West Road, Chaoyang District, Beijing; Date of deposit: 18 April 2018; Deposit No.: CGMCC No. 15623; Taxonomic name: Bacillus coagulans.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, and in particular relates to a novel thermophilic lactic acid bacterium and its application in wet corn soaking. Background technique [0002] The traditional process uses old pulp and new acid (about 0.2% sulfurous acid solution) to soak together in the soaking tank. The complete soaking process includes adding old pulp to the new tank, adding new acid to corn that has been soaked for a long time, and tank to tank There is a regular cycle in between. This circulation process can maintain a certain concentration difference between the corn and the soluble substances in the soaking liquid, and soak the soluble substances in the corn. However, the sulfur dioxide produced in the traditional immersion process is the main component of acid rain, which will cause environmental pollution. At the same time, sulfurous acid will corrode equipment and pipelines and increase production costs. In t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C08B30/04C12R1/07
CPCC08B30/044C12N1/04C12N1/20C12N1/205C12R2001/07
Inventor 刘庆艾史建国马耀宏马恒公维丽蔡雷郑岚杨俊慧
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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