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Lactobacillus freeze-dried product and preparation method of same

A technology of Lactobacillus and Lactobacillus acidophilus, applied in biochemical equipment and methods, methods based on microorganisms, bacteria, etc., can solve the problems of low content of viable bacteria, poor stability, and high mortality of bacteria, and achieve the goal of living bacteria High number, good stability, and the effect of improving the survival rate

Active Publication Date: 2013-03-20
中创益科(上海)生物科技有限公司
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Lactobacillus acidophilus, Lactobacillus paracasei or Lactobacillus rhamnosus are all lactic acid bacteria, Gram staining positive, no bacillus, in the production of their freeze-dried preparations, there are some bacteria with high death rate and poor stability. Disadvantages, resulting in low content of viable bacteria in the product, large dosage, poor fermentation effect, and high manufacturing and use costs
In addition, the composition and dosage of high-density fermentation medium are unreasonable and lack of pertinence, which is not conducive to high-density fermentation and the preparation of freeze-dried products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, A medium suitable for high-density cultivation of Lactobacillus acidophilus, Lactobacillus paracasei or Lactobacillus rhamnosus, the medium is prepared from components, 80g skimmed milk powder, 50g beet molasses, 40g corn Starch syrup, 0.50g of yeast extract or yeast powder, 12mg of uracil and xanthine, 0.005mg of biotin and folic acid, 2.0mg of niacin and calcium pantothenate, 2.0mg of riboflavin, 1.0mg of thiamine hydrochloride , 0.01g of formic acid, 20g of lactose and 50g of wort were added to distilled water and the volume was adjusted to 1000mL;

Embodiment 2

[0028] Embodiment 2, A medium suitable for high-density cultivation of Lactobacillus acidophilus, Lactobacillus paracasei or Lactobacillus rhamnosus, the medium is prepared from components, 120g of skimmed milk powder, 70g of beet molasses, 60g of corn Starch syrup, 0.70g of yeast extract or yeast powder, 12mg of uracil and xanthine, 0.005mg of biotin and folic acid, 2.0mg of niacin and calcium pantothenate, 2.0mg of riboflavin, 1.0mg of thiamine hydrochloride , 0.01g of formic acid, 20g of lactose and 50g of wort were added to distilled water and adjusted to 1000mL; wherein, the ratio of uracil and xanthine was 1:2; the ratio of biotin and folic acid was 1:1; nicotinic acid and The ratio of calcium pantothenate is 1:3.

Embodiment 3

[0029] Embodiment 3, The preparation method of Lactobacillus acidophilus, Lactobacillus paracasei or Lactobacillus rhamnosus freeze-dried product is carried out according to the following steps:

[0030] 1. High density culture

[0031] The activated bacterial strain was inoculated in a sterilized medium with an inoculation amount of 2% for fermentation; the pH in the fermentation process was two-stage, and the total fermentation time was 10 hours. The ammonia water was controlled at 6.0, and the fermentation was 7.0 hours; the pH of the fermentation liquid was not controlled in the second stage, and the fermentation was 3.0 hours; the three-stage variable temperature culture mode was adopted in the fermentation process, and the fermentation temperature was controlled at 42.5°C in the first stage, and the fermentation was 3.5 hours; In the second stage, the fermentation temperature is controlled at 35°C and fermented for 3.5 hours; in the third stage, the fermentation temper...

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PUM

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Abstract

The invention relates to a preparation method of a freeze-dried powder preparation of Lactobacillus acidophilus, Lactobacillus paracasei or lactobacillus rhamnosus, and provides a culture medium used in the freeze-drying process of Lactobacillus acidophilus, Lactobacillus paracasei or lactobacillus rhamnosus products. The inventor combines two critical factors influencing bacterial fermentation together, and creatively carries out fermentation in a high-density fermentation process by means of combination of two-sectional pH and three-sectional temperature variation, so that the relatively single form of the maximum cell biomass and the highest fermentation activity is improved remarkably, meanwhile, the obtained freeze-dried preparation is high in viable count and excellent in stability. The invention also provides an excellent freeze-drying protective additive.

Description

technical field [0001] The invention relates to a preparation method of a freeze-dried powder preparation of Lactobacillus acidophilus, Lactobacillus paracasei or Lactobacillus rhamnosus. Background technique [0002] Lactobacillus acidophilus, Lactobacillus paracasei or Lactobacillus rhamnosus are all lactic acid bacteria, Gram staining positive, no bacillus, in the production of their freeze-dried preparations, there are some bacteria with high death rate and poor stability. The disadvantages lead to less live bacteria content in the product, large dosage, poor fermentation effect, and higher manufacturing and use costs. In addition, the composition and dosage of high-density fermentation medium are unreasonable and lack of pertinence, which is not conducive to high-density fermentation and the preparation of freeze-dried products. [0003] The purpose of the present invention is to solve the above problems, and to provide a medium suitable for high-density cultivation o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/23C12R1/225
Inventor 张兰威韩雪冯镇易华西杜明张英春张莉丽单毓娟焦月华
Owner 中创益科(上海)生物科技有限公司
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