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Probiotics microcapsule wall material and probiotics microcapsule preparation method

A probiotics and microcapsule technology, applied in the field of probiotics micro-preparation, can solve the problem of difficult selection of wall materials, and achieve the effects of reduced loss, no toxic side effects, and increased system viscosity

Inactive Publication Date: 2018-07-27
GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the wall material is difficult to choose, especially the wall material that is both enteric and harmless to the human body is relatively rare

Method used

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  • Probiotics microcapsule wall material and probiotics microcapsule preparation method
  • Probiotics microcapsule wall material and probiotics microcapsule preparation method

Examples

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Embodiment 1

[0042] Such as figure 1 Shown, a kind of preparation method of the bifidobacteria microcapsule that adds oligosaccharide core material and dietary fiber wall material of the present embodiment, comprises the following steps:

[0043] (1) Activation of the strain

[0044] Seeds stored in glycerol tubes of Bifidobacteria stored at -20°C were inoculated into MRS liquid medium sterilized at 121°C for 15 minutes and cooled, and cultured statically at 37°C for 36 hours to obtain activated seed liquid.

[0045] The bifidobacterium in this example is Bifidobacterium animalis BB-12, which was purchased from Ketsen Company, Denmark.

[0046] (2) Expansion of training

[0047] The initial activated bifidobacterium culture solution was inserted into the MRS liquid medium for activation at a ratio of 3% (mass concentration), and cultured at 37° C. for 24 hours to obtain a bifidobacterium culture.

[0048] (3) Preparation of bacterial suspension

[0049]Centrifuge (4100r / min, 10min) the...

Embodiment 2

[0101] A kind of preparation method of the bifidobacteria microcapsule that adds oligosaccharide core material and dietary fiber wall material of the present embodiment, comprises the following steps:

[0102] (1) Activation of the strain

[0103] Seeds stored in glycerol tubes of Bifidobacteria stored at -20°C were inoculated into MRS liquid medium sterilized at 122°C for 20 minutes and cooled, and cultured statically at 35°C for 36 hours to obtain activated seed liquid.

[0104] The bifidobacterium in this example is Bifidobacterium animalis BB-12, which was purchased from Ketsen Company, Denmark.

[0105] (2) Expansion of training

[0106] The initial activated bifidobacterium culture solution was inserted into the MRS liquid medium for activation at a ratio of 5% (mass concentration), and cultured at 35° C. for 24 hours to obtain a bifidobacterium culture.

[0107] (3) Preparation of bacterial suspension

[0108] Centrifuge (4000r / min, 30min) the bifidobacterium strains...

Embodiment 3

[0124] A kind of preparation method of the bifidobacteria microcapsule that adds oligosaccharide core material and dietary fiber wall material of the present embodiment, comprises the following steps:

[0125] (1) Activation of the strain

[0126] Seeds stored in glycerol tubes of Bifidobacteria stored at -20°C were inoculated into MRS liquid medium sterilized at 120°C for 15 minutes and cooled, and cultured statically at 36°C for 36 hours to obtain activated seed liquid.

[0127] The bifidobacterium in this example is Bifidobacterium animalis BB-12, which was purchased from Ketsen Company, Denmark.

[0128] (2) Expansion of training

[0129] The initial activated bifidobacterium culture solution was inserted into the MRS liquid medium for activation at a ratio of 1% (mass concentration), and cultured at 36° C. for 24 hours to obtain a bifidobacterium culture.

[0130] (3) Preparation of bacterial suspension

[0131] Centrifuge (4000r / min, 30min) the bifidobacterium strains...

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Abstract

The invention discloses a probiotics microcapsule wall material preparation method. The probiotics microcapsule wall material preparation method comprises the following steps: mixing sodium alginate,carrageenan and meal fiber, performing ultraviolet lamp irradiation for 15-30 min, mixing a mixture with an aseptic phosphoric acid buffer solution with a pH value being with 6.8-7.4, performing water-bath dissolving at the temperature of 70-85 DEG C to prepare a wall material solution with mass concentration being 8.5-10.5%, wherein in the wall material solution, the mass concentration of the meal fiber is 1-5%, the mass concentration of sodium alginate is 2-4%, and the mass concentration of carrageenan is 4.5-6.5%. The invention also discloses the preparation method of the probiotics microcapsule, the volume ratio of an oligosaccharide solution (the mass concentration is 3-5%) to a probiotics suspension (greater than 5*107 CFU / mL) is 1:2-2:1. The probiotics microcapsule has the advantages of high embedding rate, high live bacteria number, high stress resistance, simple operation, economic performance, easy operation, and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of micro-preparation of probiotics, in particular to a wall material of probiotic microcapsules and a preparation method of the probiotic microcapsules. Background technique [0002] Probiotics, also known as microecological preparations and prebiotics, are microbial additives that exert beneficial effects on the host by regulating and improving the intestinal microecological balance. Probiotics are broadly classified into the genera Bifidobacterium, Lactobacillus and Gram-positive cocci. Among them, Bifidobacterium is a class of Gram-positive bacilli and is a common probiotic in the intestines of humans and other warm-blooded animals. [0003] Studies have found that bifidobacteria are closely related to many pathological and physiological phenomena of human beings, and are one of the important symbols of human health. They have important physiological and health functions for the human body, including: ① ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C9/13A23L29/00A23L33/125A23L33/135A23L33/21
CPCA23C9/13A23C9/1307A23L29/03A23L33/125A23L33/135A23L33/21A23V2002/00A23V2200/3202A23V2200/3204A23V2250/5036
Inventor 杨艺超陈骁熠邓玉娣霍霭倩黎锶华吉祥瑞袁宁儿刘丽
Owner GUANGZHOU MEDICAL UNIV
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