Method for measuring virulence by feeding Bemisia tabaci Gennadius imago

A technology for bemisia tabaci and adults, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of mesophyll damage and withering, easy mold of agar, high probability of bemisia tabaci sticking to agar, etc., to achieve The effect of avoiding death, prolonging the fresh-keeping period, and avoiding non-toxic death

Inactive Publication Date: 2011-05-04
ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for the traditional agar moisturizing leaf dipping method, which is prone to moldy agar, easily causes mesophyll damage and withering after the leaves are cut into circular leaf disks, and has a high probability of bemisia tabaci sticking to the agar. A feeding method for whitefly adults that can more realistically simulate the growth of leaves, prolong the fresh-keeping period of leaves, reduce the agar contamination rate and improve the safety of test insects, so that test insects can achieve standardization and ensure the reliability of experimental results

Method used

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  • Method for measuring virulence by feeding Bemisia tabaci Gennadius imago

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: (poinsettia raises whitefly adult and carries out the method for virulence determination)

[0020] (1) Preparation and sterilization of synthetic medium: Weigh 7g of agar, heat it, dissolve it in 500mL of distilled water with stirring, and prepare an agar solution; then add 25mL of NH 4 NO 3 20mg / L, KNO 3 3mg / L, MgSO 4 ·7H 2 O2mg / L, KH 2 PO 4 2mg / L, anhydrous CaCl 2 1 mg / L and make up to 1 liter of nutrient solution with water, and stir evenly to form a synthetic medium; after autoclaving the synthetic medium at 121°C for 15-20 minutes, pour it separately on the ultra-clean workbench Put it into a sterilized flat-bottom glass test tube with a length×diameter×thickness of 30mm×25mm×1.5mm, put the glass test tube into a sterilized and dried wide-mouth glass bottle after cooling, seal it with sterile plastic paper, and set aside ;

[0021] (2) Chemical treatment of leaves, petiole disinfection and insertion: cut the fresh and tender leaves of the test...

Embodiment 2

[0024] Embodiment 2: (the eggplant raises the whitefly adult and carries out the method for virulence determination)

[0025] In this example, the preparation of the synthetic medium in step (1) is as follows: weigh 7.5g of agar and dissolve it in 500mL of distilled water to prepare an agar solution; then add 27.5mL of NH 4 NO 3 22.5mg / L, KNO 3 2mg / L, MgSO 4 ·7H 2 O 1mg / L, KH 2 PO 4 3mg / L, anhydrous CaCl 22 mg / L and make up to 1 liter of nutrient solution with water, stir evenly to form a synthetic medium; step (2) chemical treatment of leaves, petiole disinfection and insertion: cut the test material eggplant leaves and use 10% imidacloprid WP 2000 After the 1-fold dilution is processed and dried in the shade, the petiole is soaked in 50% carbendazim wettable powder 800-fold dilution for 10-20 seconds for disinfection; the remaining steps and processes are the same as in Example 1.

Embodiment 3

[0026] Embodiment 3: (the method that cotton raises Bemisia tabaci adults and carries out virulence assay)

[0027] In this example, the preparation of the synthetic medium in step (1) is as follows: weigh 8 g of agar and dissolve it in 500 mL of distilled water to prepare an agar solution; then add 30 mL of NH 4 NO 3 25mg / L, KNO 3 1mg / L, MgSO 4 ·7H 2 O3mg / L, KH 2 PO 4 1mg / L, anhydrous CaCl 2 3 mg / L and make up to 1 liter of nutrient solution with water, stir evenly to form a synthetic medium; step (2) chemical treatment of leaves, petiole disinfection and insertion: cut test material cotton leaves and use 10% imidacloprid WP 3000 After the 1-fold dilution is processed and dried in the shade, the petiole is soaked in 1000-fold dilution of 50% carbendazim wettable powder for 10-20 seconds for disinfection; the remaining steps and processes are the same as in Example 1.

[0028] Table 1 Synthetic culture medium mildew and leaf preservation

[0029]

[0030] Annotate: ...

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Abstract

The invention discloses a method for measuring virulence by feeding Bemisia tabaci Gennadius imago, belonging to the technical field of insect feeding. The method comprises: (1) preparing and sterilizing a synthetic medium; (2) processing leaves by a medicament, and disinfecting and planting petioles; (3) adsorbing and releasing the Bemisia tabaci Gennadius imago; and (4) feeding, observing and the like. The invention can truly simulate the growing situation of leaves on plants, and can prolong the freshness of the leaves, and the medium has small possibility of going mouldy, thus effectively preventing the test imago of the Bemisia tabaci Gennadius from dying in a non-virulence mode; the contrast Bemisia tabaci Gennadius can normally survive within 72 hours of normal virulence measurement to obtain the standardization of the experiment test imago. The method is suitable for the requirements on measuring virulence by feeding Bemisia tabaci Gennadius imago for flowers, vegetables and cash crops.

Description

technical field [0001] The invention relates to the technical field of insect feeding, in particular to a feeding method for adult Bemisia tabaci used for biological determination. Background technique [0002] Bemisia tabaci (Gennadius) belongs to Homoptera Aleyrodidae, which is native to North Africa and the Middle East, and is one of the main pests of tropical and subtropical protected areas and field crops (Brown et al., 1995). Bemisia tabaci mainly damage the normal growth of plants by sucking plant juice, secreting honeydew, causing mold to pollute the leaf surface, affecting photosynthesis, spreading plant viruses, and inducing abnormal physiological responses of crops (Davidson et al., 2000). The harm caused by the disease is much more serious than other harms (Feng Lanxiang et al., 2001), and the loss of food and economic crops caused by its harm can reach 30-100% (Brown, 1994). [0003] As of 2005, there were at least 26 biotypes of B. tabaci described in the worl...

Claims

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Application Information

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IPC IPC(8): A01K67/033A01H4/00
Inventor 韦茂兔黄俊
Owner ZHEJIANG XIAOSHAN COTTON & FLAX RES INST
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