42 results about "Fecal microbiota" patented technology

The invention belongs to the technical field of fecal microbiota transplantation, and particularly relates to a fecal microbiota capsule, as well as a preparation method and application of the fecal microbiota capsule. The preparation method comprises the following steps: (1) a fecal microbiota solution is prepared; (2) the fecal microbiota solution, obtained in the step (1), is added with a freeze-drying protective additive, and freeze drying is performed to obtain fecal microbiota freeze-dried powder; (3) a capsule is filled with the fecal microbiota freeze-dried powder, obtained in the step (2), to obtain the fecal microbiota capsule. Through adoption of the fecal microbiota capsule, flora is enabled to be comparatively concentrated and can be preserved for a long time at a low temperature; implementation of flora transplantation is facilitated, and invasive implementation manners, such as a gastroscope, an enteroscope and a nasointestinal feeding tube, are not needed; when the fecal microbiota capsule is applied to weak patients, the safety is increased.

In alternative embodiments, the invention provides compositions and methods for treating various disorders and conditions in mammals, including chronic disorders in which there is a presence of an abnormal microbiota or an abnormal distribution of microflora in the gastrointestinal tract. In alternative embodiments, the invention provides liquid preparations or formulations derived from a human fecal material (e.g., a stool) processed, e.g., filtered and / or centrifuged, such that all bacteria, fungal spores and viruses are removed, but retaining the native biologically active molecules from the fecal material and bacteriophages. In alternative embodiments, the invention provides a "rough-", "incomplete-" or medium- filtered microbiota which still comprises native physiological components or nutritive agents for the bacteria, e.g., retains native biologically and nutritionally active components. In alternative embodiments, the invention provides a highly filtered or substantially purified microbiota in combination with, or having added back, a liquid preparation or formulation of the invention. In alternative embodiments, the invention provides compositions or formulations where the bacteria, or microbiota, component has been cultured, or cultured under anaerobic conditions, or harvested, stored and / or cultured under anaerobic conditions. In alternative embodiments, the invention provides various additives, compositions and donor restrictions for treating these disorders and conditions.

The present invention provides freeze-dried compositions that include an extract of human feces and a cryoprotectant, and methods for making and using such compositions, including methods for replacing or supplementing or modifying a subject's colon microbiota, and methods for treating a disease, pathological condition, and / or iatrogenic condition of the colon.

The invention discloses a medicament and intestinal flora cooperation researching method based on feces filtering and transplanting. The method comprises the following steps of 1, preparing a fecal microbiota transplantation acceptor experiment animal, wherein a pseudo-germfree or a sterile animal is selected as the fecal microbiota transplantation acceptor experiment animal, thereby creating an environment without bacteria or little bacteria in an intestinal tract, and facilitating colonization of external microbes; 2, preparing a fecal microbiota transplantation denor experiment animal, wherein the denor animal receives disease modeling and medicament gavage processing, and establishing a positive and negative experiment contrast of the drug effect; and 3, making the acceptor experimentanimal receive filtering and non-filtering fecal microbiota transplantation of the donor experiment animal. The method according to the invention is based on a typical fecal microbiota transplantationintestinal flora researching method and further realizes advantages of simple operation and easy application. The method further has advantages of eliminating direct influence of drug to the intestinal tract, focusing a flora mediation medicament function and supplying a concise method for researching interacting between the medicament-modulated flora and a host.

This document relates to biomarkers of gut microbiota dysbiosis. Bacteria that are increased or decreased in gut microbiota dysbiosis can be used as biomarkers to predict dysbiosis in patients with diarrhea and / or to predict susceptibility to Clostridium difficile infection (CDI). In addition, provided herein are compositions including at least three bacteria that are de creased in gut microbiota dysbiosis which can be used, for example, to restore heathy gut microbiota (e.g., by probiotic or by fecal microbiota transplant) to treat CDI.

The invention belongs to the field of microorganisms, and particularly relates to a freeze-drying protective agent, a fecal microbiota freeze-dried product and a preparation method of the freeze-drying protective agent. The freeze-drying protective agent comprises polydextrose, mannitol, glycine, vitamin C and Tween 80; the freeze-drying fecal microbiota product prepared from the freeze-drying protective agent is high in fecal microbiota total fungus survival rate and superior to trehalose in effect; and the potential risk caused by trehalose, maltodextrin and the like can be reduced, and thefinal curative effect of the fecal microbiota transplanting product can be potentially improved.

The present disclosure provides compositions and methods for treating Clostridium difficile infection (CDI) including primary and recurrent CDI. In particular, the compositions and methods described herein are capable of achieving a CDI clearance rate of at least 80% through a single oral dose of a pharmaceutical composition comprising a freeze-dried fecal microbiota preparation.

The invention belongs to the technical field of fecal microbiota transplantation, and particularly relates to a fecal microbiota capsule, as well as a preparation method and application of the fecal microbiota capsule. The preparation method comprises the following steps: (1) a fecal microbiota solution is prepared; (2) the fecal microbiota solution, obtained in the step (1), is added with a freeze-drying protective additive, and freeze drying is performed to obtain fecal microbiota freeze-dried powder; (3) a capsule is filled with the fecal microbiota freeze-dried powder, obtained in the step (2), to obtain the fecal microbiota capsule. Through adoption of the fecal microbiota capsule, flora is enabled to be comparatively concentrated and can be preserved for a long time at a low temperature; implementation of flora transplantation is facilitated, and invasive implementation manners, such as a gastroscope, an enteroscope and a nasointestinal feeding tube, are not needed; when the fecal microbiota capsule is applied to weak patients, the safety is increased.

The invention relates to a fecal microbiota collection, cryopreservation and unfreezing method of a fecal microbiota transplantation technology. The method comprises the following steps: (1) preparingfecal microbiota; (2) carrying out vitrification freezing on the fecal microbiota prepared in the step (1) to obtain cryopreserved fecal microbiota; and (3) unfreezing and recovering the cryopreserved fecal microbiota obtained in the step (2). According to the invention, a vitrification freezing technology is adopted, so that the freezing rate (greater than 20000 DEG C / min) is greatly increased,cells rapidly form a 'glassy state' in a rapid cooling process, ice crystals are prevented from being formed inside and outside the cells, excessive damage to florae in the fecal microbiota is avoided, and an antifreezing protein is added into a cryoprotectant, so that the cytotoxicity of the cryoprotectant is avoided; and the survival rate of the flora in the unfrozen fecal microbiota liquid is increased to 90% or above.

The invention relates to the use of fecal microbiota transplant for preventing and / or reducing systemic and gut treatment-induced inflammation in an individual in need thereof.

The invention relates to a fecal microbiota sample for use in the prevention and / or treatment of infectious or non-infectious complications resulting from allogeneic hematopoietic stem cell (HSC) transplantation, or for the treatment of cancer in a recipient patient, said fecal microbiota sample and said hematopoietic stem cells originating from the same donor subject, or said fecal microbiota sample being administered prior to HSC transplantation.

The invention relates to a method for preparing a fecal microbiota transplantation capsule containing benzoylaconine by utilizing human fecal microbiota fermented aconite roots. Fecal microbiota transplantation (FMT) means that a functional flora in healthy human feces is transplanted into gastrointestinal tracts of a patient to re-establish a new intestinal flora so as to realize treatment on intestinal and parenteral diseases. Clinical application discovers that a required curative effect cannot be achieved after certain patients take the prepared aconite roots, one of main reasons causing individual differences is incomplete intestinal flora of the patient, and toxic substances in the prepared aconite roots cannot be normally metabolized into non-toxic active ingredients, the active ingredients have low utilization, and the required curative effect of the prepared aconite roots cannot be achieved. Healthy high-quality feces of a fecal microbiota transplant donor are utilized, are used for simulating intestinal microbes in vitro to achieve an effect of transforming the prepared aconite roots, aconitine and other toxic ingredients in the prepared aconite roots can be transformed into nontoxic metabolitic products through proper fermentation conditions, and the content of benzoylaconine and other active ingredients is increased. The fecal microbiota capsules prepared from fecalmicrobiota fermentation liquid can be used in fecal microbiota transplantation treatment in clinical application.

The invention discloses a preparation method and application of fecal microbiota liquid and a preparation method and application of fecal microbiota freeze-dried powder, and belongs to the technical field of fecal microbiota transplantation. The preparation method of the fecal microbiota liquid and the preparation method of the fecal microbiota freeze-dried powder comprise the following steps: preparing original microbiota liquid; performing centrifuging and washing; preparing the fecal microbiota liquid; and preparing the fecal microbiota liquid freeze-dried powder. The fecal microbiota liquid and the freeze-dried powder thereof prepared by the preparation method disclosed by the invention are used for preparing medicines for treating ulcerative colitis. The fecal microbiota freeze-driedpowder prepared by the invention is concentrated in flora and can be stored for a long time at low temperature. Through washing treatment, the toxicity of fecal microbiota is reduced or removed. A freeze-dried powder technology is adopted, so that implementation of flora transplantation is facilitated, and the operation is convenient.