Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Technology for preparing intestinal flora preparation in fecal microbiota transplantation clinic treatment

A technology of intestinal flora and clinical treatment, applied in the direction of non-active ingredient medical preparations, medical preparations containing active ingredients, digestive system, etc., can solve the problems of intestinal flora loss, death, high equipment requirements, etc. Achieve the effect of maintaining bacterial activity, prolonging storage time, and protecting diversity

Inactive Publication Date: 2018-12-18
卓源健康科技有限公司
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the mainstream methods of preparing intestinal flora preparations on the market are mainly divided into two types: aerobic separation and anaerobic separation. Aerobic separation is easy to operate and simple in equipment, and is widely used by many people. Most of the flora are anaerobic bacteria, and some of them are strictly anaerobic bacteria. They are easy to lose their activity, die and decompose in an aerobic environment. Therefore, the method of completely aerobic separation causes serious loss of intestinal flora
Complete anaerobic separation method, complex operation, high equipment requirements, and will lose part of the aerobic flora

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] An intestinal flora preparation in the clinical treatment of fecal bacteria transplantation, the preparation steps of which are as follows:

[0033] 1) Stool collection:

[0034] When receiving the notification of the donor’s defecation, prepare the collection box and put it in the stool collection room for sterilizing with the ultraviolet lamp for 30 minutes, and spray alcohol for disinfection. Defecate above the collection box. After the defecation is completed, seal the collection box with a homogeneous sealed bag, and transport it to the laboratory in a cold chain at 4°C for processing;

[0035] 2) Preparation of intestinal flora preparation:

[0036] After weighing the feces, record the feces properties, weight, and date, and evaluate the quality of the feces, collect high-quality feces and transfer them to the anaerobic workstation for processing, take 90g of high-quality feces, and divide them into 60g and 60g in the anaerobic workstation. 30g in two parts, tra...

Embodiment 2

[0043] An intestinal flora preparation in the clinical treatment of fecal bacteria transplantation, the preparation steps of which are as follows:

[0044] 1) Stool collection:

[0045] When receiving the notification of the donor’s defecation, prepare the collection box and put it in the stool collection room for sterilizing with the ultraviolet lamp for 30 minutes, and spray alcohol for disinfection. Defecate above the collection box. After the defecation is completed, seal the collection box with a homogeneous sealed bag, and transport it to the laboratory in a cold chain at 4°C for processing;

[0046] 2) Preparation of intestinal flora preparation:

[0047] After weighing the feces, record the feces properties, weight, and date, and evaluate the quality of the feces, collect high-quality feces and transfer them to the anaerobic workstation for processing, take 90g of high-quality feces, and divide them into 60g and 60g in the anaerobic workstation. 30g in two parts, tra...

Embodiment 3

[0054] An intestinal flora preparation in the clinical treatment of fecal bacteria transplantation, the preparation steps of which are as follows:

[0055] 1) Stool collection:

[0056] When receiving the notification of the donor’s defecation, prepare the collection box and put it in the stool collection room for sterilizing with the ultraviolet lamp for 30 minutes, and spray alcohol for disinfection. Defecate above the collection box. After the defecation is completed, seal the collection box with a homogeneous sealed bag, and transport it to the laboratory in a cold chain at 4°C for processing;

[0057] 2) Preparation of intestinal flora preparation:

[0058] After weighing the feces, record the feces properties, weight, and date, and evaluate the quality of the feces, collect high-quality feces and transfer them to the anaerobic workstation for processing, take 90g of high-quality feces, and divide them into 60g and 60g in the anaerobic workstation. 30g in two parts, tra...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a technology for preparing an intestinal flora preparation in fecal microbiota transplantation clinic treatment. The technology comprises the following steps: collecting feces,preparing intestinal flora, and filling the intestinal flora preparation, wherein the step of collecting feces includes fecal collection and anaerobic transportation, the step of preparing the intestinal flora preparation includes stepwise filtration under aerobic and anaerobic conditions, centrifuging and mixing, and the step of filling the intestinal flora preparation adopts storage using a glycerol protection solution and a cryopreservation technology. A novel aerobic and anaerobic separation method is adopted in the separation process of an intestinal flora solution, the aerobic and anaerobic separation method selected according to the characteristics of the living environment of intestinal florae can maximally protect the flora diversity of aerobic bacteria and anaerobic bacteria inthe intestinal tract, and an added glycerol protection agent maintains the bacterial activity within a certain period of time and improves the effect of the intestinal flora preparation.

Description

technical field [0001] The invention relates to the technical field of medical preparation technology, in particular to a technology of intestinal flora preparation in the clinical treatment of fecal bacteria transplantation. Background technique [0002] Modern scientific research shows that intestinal flora plays a vital role in human health. The intestinal flora of the human body maintains a dynamic balance with the intestinal environment. In some cases, this balance is broken, and the intestinal flora is out of balance, causing diseases or aggravating diseases, causing complications and even multiple organ dysfunction syndrome and multiple organ failure. [0003] Intestinal flora transplantation, also known as fecal microbiota transplantation (FMT for short), is to isolate the functional flora in the feces of healthy people and transplant them into the intestinal tract of patients through gastrointestinal endoscopy, colonoscopy, enema, etc., to rebuild the patient's In...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/741A61K47/10A61P1/00
CPCA61K35/741A61K47/10A61P1/00
Inventor 张彬郭建姜秋吴亮孙金品金泽宇
Owner 卓源健康科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com