The invention provides a high-efficiency compound microbial inoculum for fermenting beds, particularly relates to a compound microbial inoculum for the fermenting beds, and a preparation method and application thereof. The compound microbial inoculum is composed of five beneficial bacterium strains (
Bacillus subtilis CGMCC1.769 and CGMCC1.210,
lactobacillus plantarum CICC6073,
Candida tropicalis CGMCC2.587 and
Trichoderma koningii CGMCC3.4290) and metabolites thereof (
amylase,
protease,
lipase,
cellulase,
organic acid,
bacteriophage and the like). The compound microbial inoculum is prepared by using a submerged liquid mixed
fermentation technique. The method comprises the following steps: carrying out liquid
submerged fermentation on the
lactobacillus plantarum at 35 DEG C for 12 hours, respectively inoculating
Candida tropicalis and
Trichoderma koningii, carrying out mixed
fermentation at 30 DEG C for 12 hours, inoculating
Bacillus subtilis, carrying out liquid submerged mixed
fermentation for 24 hours, respectively controlling the fermented
viable count at 1-2*10<9> cfu / mL, 5-8*10<8> cfu / mL, 3-5*10<8> cfu / mL, 3-5*10<9> cfu / mL and 2-5*10<9> cfu / mL, finishing fermentation, packaging and sealing to obtain the high-efficiency compound microbial inoculum for the fermenting beds. The invention also provides application of the high-efficiency compound microbial inoculum-a preparation method of padding material. The compound microbial inoculum has the advantages of high
viable count, high
biological activity and low
impurity bacterium
pollution; the compound microbial inoculum can obviously reduce the
odor of pigsties and reduce the living possibility of mosquitoes and flies, thereby achieving the ecological culture goals of no
pollution and zero
discharge; and the process for manufacturing the ferment
bed is simple, thereby saving the padding material,
human power and the like.