The invention discloses a sterilization method of fruit and vegetable juice. The sterilization method comprises the following steps: subjecting fruit and vegetable raw materials to pretreating, stripping and slicing and cold pressing: selecting fresh, moderately-ripe and non-damaged fruit and vegetable materials, carrying out cleaning, stripping and slicing the cleaned materials into 1-3 cm<3> insize, and carrying out cold pressing at 0-8 DEG C with pulp temperature controlled at 15 DEG C or below; carrying out a high-pressure homogenization treatment: pressure 120-200 MPa, feeding temperature 25-40 DEG C and homogenization times 1-3 times, and cooling the materials to 4 DEG C after the high-pressure homogenization treatment; carrying out thermosonication treatment: power 200-600 W, temperature 45-58 DEG C and time 5-30 minutes. According to the invention, research findings show that the high-pressure homogenization treatment can synergize with the thermosonication treatment, with thewhole processing temperature being 52 DEG C or below and the total processing time being only 5 minutes, so that the sterilization method ensures sensory characteristics, quality, solid matter contents, betacyanins, total sugar, fruit grain suspension stability and other characteristics of the fruit and vegetable juice, and has a good sterilizing effect to completely kill pathogenic bacteria at the same time.