Microalga strain with high CO2 tolerance and high fixation rate and breeding method thereof

Abstract

The invention belongs to the technical field of microalga biology, and particularly relates to a microalga strain with high CO2 tolerance and high fixation rate and a breeding method thereof. The microalga is named Chlorella sp.Y-1, and is collected into Common Microorganism Center of China General Microbiological Culture Collection Committee; and collection number is CGMCC No.5429. The invention has the following characteristics: the initial strain is separated from natural environment; after high-CO2-concentration enrichment culture, gene cloning, induced mutation breeding and other operations, the carbon content of the target strain is increased to 56.981%, the optimal fixation concentration is up to 20% (v / v), the fixation rate is up to 5.762g / (L.d), and the maximum tolerance concentration is 100% (v / v); and the target strain has high adaptability to a series of physical and chemical culture conditions, has high culture transfer stability, and high better overall properties than the like research findings at present. The invention aims to solve the bottleneck problem in high concentration CO2 biological fixation technology, cultures an efficient organism capable of efficiently fixing high concentration CO2, implements effective fixation of CO2 in high concentration flue gas and other complex environments, and meanwhile, generates beneficial biological substances.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, in particular to a plant with high CO 2 Microalgae for tolerance and fixation rates and methods for breeding them. Background technique [0002] Over the past century, the global temperature has increased significantly, which has had a negative impact on the natural ecosystems in many areas, and even led to irreparable damage. As warming increases, so does the number and geographic extent of damaged ecosystems. A large number of studies have shown that global warming and the resulting adverse consequences are mainly due to CO emissions from human activities. 2 greenhouse effect of greenhouse gases. CO 2 has been the main greenhouse gas emitted by human activities, therefore, CO 2 It will inevitably become the focus of current greenhouse gas emission reduction control. [0003] my country is a country with a large population, energy production and consumption in the world, and the amo...

AI Technical Summary

Problems solved by technology

[0006] "A Strain of Chlorella and Its Application" (201019026021.X) by Huang He et al., the isolated Chlorella M209256 has the highest CO 2 Tolerated concentration is only 24.5% (v / v), optimal CO 2 The fixed concentration is 5% (v / v), and the optimal biomass in 15 days is 2.23g / L, calculated according to the carbon content of 50% of common Chlorella, the optimal CO 2 The fixation rate is 4.088g / L, equivalent to 0.273g / (L·d), in CO 2 CO at a concentration of 24.5% (v / v) 2 The fixation rate is 2.05g / L, which is equivalent to 0.137g / (L d); Liu Junhan et al. "A strain of Chlorella and its cultivation method and application" (201110145547.7), this method focuses on obtaining multifunctional Chlorella , the method described for Chlorella M4653 with the highest CO 2 The tolerance concentration is 30% (v / v), preferably at 5% (v / v) CO 2 When the oil content was 33.5% and the output was 1.51g / L, it was only 24.7% and 0.79g / L when the oil was 20%. Obviously, this algae is not suitable for high CO 2 concentration environment, and there is no suitable CO for the algae in the method 2 concentration and optimal CO 2 fixed situation

Images