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Method for processing fish collagen oligopeptide

A processing method and oligopeptide technology, applied in protein food processing, animal protein processing, pharmaceutical formulations, etc., can solve problems such as non-recyclability, high salt content in products, and residual fishy smell, so as to avoid waste of resources, enzymes, etc. The effect of short solution time and no side effects

Active Publication Date: 2008-11-05
BEIJING SEMNL BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of enzyme engineering technology to produce fish collagen is an advanced method commonly used at home and abroad, but the current processing methods mainly have the following problems: 1. Activated carbon is used for decolorization in the production process, and the products produced have high ash content and cannot be recycled; 2. Hydrolysis In the process, industrial grade hydrochloric acid and sodium hydroxide are used to adjust the pH value, and the final quality of the processed product cannot meet the requirements of food grade; 3. The aqueous solution of the processed product is not colorless, and there is a fishy smell; 4. The salt content of the product high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 10 kg of fish scales that have been delimed and degreased, put them into the reaction tank, add 5 times of deionized water at room temperature to wash, stir for 15 minutes to drain the water and impurities, and then add 3 times of deionized water at room temperature to the fish scales after cleaning and stirring. After stirring for 15 minutes, drain the water and impurities, feed according to 8% of the dry weight of raw materials, add deionized water with Ph=6.0, stir evenly, heat up to 90°C and keep it for 10 minutes, then cool down to 53°C, the Ph of the solution is just right Equal to 5.6, add 10 grams of papain for enzymolysis for 3 hours, then heat up to 90°C, keep warm for 10 minutes to inactivate the enzyme, then use a diatomite filter to filter, the diatomite is 200 mesh, and the pressure during the filtration process is 0.3MP. The filtered supernatant is put into the storage tank, and then the supernatant is decolorized, deodorized and desalted through mac...

Embodiment 2

[0032]Weigh 560 kg of fish scales that have been deashed and degreased, put them into the reaction tank, add 7 times the deionized water at room temperature to wash, stir for 20 minutes to drain the water and impurities, and add 4 times the deionized water at room temperature to the fish scales after cleaning and stirring. Water, after stirring for 20 minutes, drain the water and impurities, feed according to 12% of the dry weight of raw materials, add deionized water with Ph=7.0, stir evenly, heat up to 90°C and keep it for 10 minutes, cool down to 60°C, and the solution Ph is exactly equal to 6.5, add 2.0 kg of alkaline protease to hydrolyze for 3 hours, then heat up to 90°C, keep warm for 10 minutes to kill the enzyme, and then filter with a diatomite filter, the diatomite is 200 mesh, and the pressure during the filtration process It is 0.5MP, and the filtered supernatant liquid is poured into the storage tank, and then the decolorization, deodorization and desalination tre...

Embodiment 3

[0034] Weigh 480 kg of fish scales that have been deashed and degreased, put them into the reaction tank, add 8 times the deionized water at room temperature to wash, stir for 30 minutes to drain the water and impurities, and add 5 times the deionized water at room temperature to the fish scales after cleaning and stirring. Water, after stirring for 30 minutes, drain the water and impurities, feed according to 15% of the dry weight of raw materials, add deionized water with Ph=7.0, stir evenly, heat up to 100°C, keep warm for 20 minutes, cool down to 65°C, The Ph of the solution is equal to 7.5, add 1.2 kg each of alkaline protease and animal protein compound hydrolase, enzymatically hydrolyze for 3 hours, then heat up to 90°C, keep warm for 10 minutes to kill the enzyme, and then filter with a diatomaceous earth filter, diatomaceous earth is 250 mesh, the pressure during the filtration process is 0.6MP, the filtered clear liquid is poured into the storage tank, and then decolo...

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Abstract

The invention discloses a processing method of fish collagen oligopeptide, which comprises the following steps: delimed and degreased dry scales are selected as raw material, after being treated with cleaning, pretreatment, enzymolysis, filtration, decolorization, taste escaping, desalinization, aseptic filtration, concentration, and spray drying, fish collagen oligopeptide powder is obtained; according to dry basis, the fish collagen oligopeptide powder contains: protein content of no less than 95 percent, ash content of less than 0.5 percent, and average molecular weight of 700Dalton. The product processed by the method of the invention has the advantages of high quality, colorlessness, no bitter taste or stink, low salt content, no poisonous or side effect, and short enzymolysis time, the resin filling used during the adsorption process is renewable and can be repeatedly used for a plurality of times, and the problems of wasting of resources and high ash content of the product caused by using the disposable activated charcoal are avoided, thus being widely applied in the fields of food, health products, medicine and cosmetics, etc.

Description

technical field [0001] The invention relates to a processing method of oligopeptides, in particular to a processing method of fish collagen oligopeptides. Background technique [0002] Collagen is widely found in connective tissues such as animal skin, bone, tendon, and membrane, and is the main component of connective tissue. In the past, the production of collagen mainly used terrestrial animals with abundant resources, such as the skin and bone of cattle and pigs, as raw materials for processing and production. Although fish collagen has long been recognized by humans, such as tortoise shell glue and yellow fish glue, etc., it has not been utilized as an important industrial application material. It is mainly a product that relies on marine fishing, and its source is unstable and lacks scale. In recent years, because terrestrial animals are affected by various factors such as the environment and infectious diseases, the safety of their proteins has been questioned, while...

Claims

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Application Information

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IPC IPC(8): A23J3/04A23J3/34A23L1/305A61K38/39A61K8/65A23L33/18
Inventor 刘爱青姜涛董峭
Owner BEIJING SEMNL BIOTECHNOLOGY CO LTD
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