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Full fecal microbiota compound capsule as well as preparation method and application thereof

A technology of composite capsules and fecal bacteria, which is applied in capsule delivery, drug combination, and pharmaceutical formulations, etc., can solve the problems of short storage time of whole fecal bacteria capsules, increased content of beneficial bacteria, and insignificant colonization effect, so as to improve the beneficial bacteria. The effect of the number of groups, increased acceptance, and significant efficacy

Inactive Publication Date: 2018-09-25
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In order to solve the problem of short storage time and insignificant colonization effect of the whole fecal bacteria capsules in the prior art, the present invention provides a whole fecal bacteria composite capsule, which has a longer storage time, significantly increased content of beneficial bacteria, and better effect after transplantation. good

Method used

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  • Full fecal microbiota compound capsule as well as preparation method and application thereof
  • Full fecal microbiota compound capsule as well as preparation method and application thereof
  • Full fecal microbiota compound capsule as well as preparation method and application thereof

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preparation example Construction

[0077] The present invention has no special limitation on the preparation method of the prebiotic, and methods known in the art can be used, such as direct mixing.

[0078] Tests show that compared with the whole fecal bacteria enteric-coated capsule provided by the invention compared with the whole fecal bacteria enteric capsules without prebiotics, Bifidobacterium, Clostridium tenebilis, Akkermansia and Lactobacillus in the whole fecal bacteria flora The contents of beneficial flora increased significantly, and the contents of main pathogenic flora such as Clostridium, Haemophilus and Biliophilus in the whole fecal flora decreased significantly. That is to say, the structure of the whole fecal bacteria in the whole fecal bacteria composite capsule provided by the present invention is more reasonable, and the therapeutic effect after being transplanted into the patient's intestinal tract is better.

[0079] Tests have shown that the whole fecal bacteria compound capsule provi...

Embodiment 1

[0092] (1) Material collection: Select healthy donors according to the criteria for healthy donors, and put the isolated fresh feces donated by healthy donors into the feces collection bucket;

[0093] (2) Bacterial solution dilution: Add 100g of isolated fresh feces into 400ml of sterile saline at 4°C for 1 minute, and stir evenly with a stirrer for about 1 minute;

[0094] (3) Filtration: Filter sequentially with a stainless steel mesh, and the mesh number of the mesh is increased step by step by 2mm, 1mm, 0.5mm, and 0.25mm to remove feces residues and large particles, and take the final filtered filtrate to obtain the whole fecal bacteria filtrate;

[0095] (4) Centrifuge the whole fecal bacteria filtrate: 3 times

[0096] The first centrifugation: 5 minutes, 2000r / min, remove the supernatant, leave the sediment (5ml), add normal saline to 45ml, shake well;

[0097] The second centrifugation: 3 minutes, 2000r / min, remove the supernatant, keep the sediment, add normal salin...

Embodiment 2

[0104] (1) Material collection: Select healthy donors according to the criteria for healthy donors, and put the isolated fresh feces donated by healthy donors into special feces collection buckets;

[0105] (2) Bacteria solution dilution: Add 100g of isolated fresh feces into 500ml of sterile saline at 4°C and soak for 1 minute, and stir evenly with a stirrer for about 1 minute;

[0106] (3) Filtration: Filter sequentially with a stainless steel screen, and the mesh number of the filter screen increases step by step by 2mm, 1mm, 0.5mm, and 0.25mm to remove fecal residues and large particles, and collect the whole fecal suspension;

[0107] (4) Centrifugal cleaning: 2 times

[0108] The first centrifugation: 5 minutes at 2500 rpm, remove the supernatant, leave the sediment (5ml), add normal saline to 45ml, shake well;

[0109] The second centrifugation: 2500 rpm for 3 minutes, remove the supernatant, leave the sediment, add normal saline to 20ml, get the whole fecal bacteria, ...

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Abstract

The invention relates to the field of medicines, and provides a full fecal microbiota compound capsule. The full fecal microbiota compound capsule comprises a wall material and a core material, wherein the wall material is an enteric capsule shell, and the core material comprises full fecal microbiota and prebiotics; the effective viable count in the full fecal microbiota compound capsule is 1*10<10>-2.88*10<10> / grain. The full fecal microbiota and the prebiotics can be taken as the core material, the characteristic that prebiotics selectivity is used for accelerating the growth and propagation of beneficial microorganisms is used for obviously improving the amount of beneficial bacteria floras in the full fecal microbiota, and the effective viable count is lowered. On one hand, the survival time of beneficial microorganisms with functions in the full fecal microbiota can be effectively improved, and on the other hand, the function effect of the full fecal microbiota compound capsule after intestinal tract planting can be improved. Therefore, the full fecal microbiota compound capsule has the obvious curative effects for patients who suffer from refractory difficult-distinguishingclostridium infection, constipation, diarrhea, Crohn's disease, ulcerative colitis, irritable bowel syndrome, food intolerance and the like.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a whole-fecal-bacteria composite capsule, a preparation method and application thereof. Background technique [0002] The gut microbial ecosystem is the most complex and largest micro-ecosystem in mammals. However, the widespread use of a large number of antibiotics and changes in diet structure have greatly changed the composition of human intestinal microbes, the normal intestinal flora has been damaged, and even Clostridium difficile infection has developed, which has led to some new the occurrence of diseases. [0003] "Fecal microbiota transplantation" (FMT) refers to the transplantation of functional flora in the feces of healthy people into the gastrointestinal tract of patients to rebuild new intestinal flora and achieve the treatment of intestinal and extraintestinal diseases. As a key salvage treatment for refractory inflammatory bowel disease (Crohn's disease, ulcerative col...

Claims

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Application Information

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IPC IPC(8): A61K35/741A61K9/48A61K47/26A61P1/00A61P1/10A61P1/04A61P1/12A61P3/04A61P3/10A61P11/06A61P17/00A61P25/00A61P25/28
CPCA61K35/741A61K9/4858A61P1/00A61P1/04A61P1/10A61P1/12A61P3/04A61P3/10A61P11/06A61P17/00A61P25/00A61P25/28
Inventor 曾强赵鹏张晓莉杨婷婷
Owner GENERAL HOSPITAL OF PLA
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