Intestinal flora researching method based on feces filtering and transplanting

Abstract

The invention discloses a medicament and intestinal flora cooperation researching method based on feces filtering and transplanting. The method comprises the following steps of 1, preparing a fecal microbiota transplantation acceptor experiment animal, wherein a pseudo-germfree or a sterile animal is selected as the fecal microbiota transplantation acceptor experiment animal, thereby creating an environment without bacteria or little bacteria in an intestinal tract, and facilitating colonization of external microbes; 2, preparing a fecal microbiota transplantation denor experiment animal, wherein the denor animal receives disease modeling and medicament gavage processing, and establishing a positive and negative experiment contrast of the drug effect; and 3, making the acceptor experimentanimal receive filtering and non-filtering fecal microbiota transplantation of the donor experiment animal. The method according to the invention is based on a typical fecal microbiota transplantationintestinal flora researching method and further realizes advantages of simple operation and easy application. The method further has advantages of eliminating direct influence of drug to the intestinal tract, focusing a flora mediation medicament function and supplying a concise method for researching interacting between the medicament-modulated flora and a host.

Description

technical field [0001] The invention relates to a step-by-step processing method for animal experiments in the research of medical intestinal flora, in particular to a research method for intestinal flora based on feces filtration and transplantation. Background technique [0002] Intestinal flora is a complex and huge group. In a healthy adult, the total weight of intestinal flora can reach about 1-2 kg, and the number of cells contained in it can reach as high as 10 13 ~10 14 One, which is about 10 times the number of human cells, and the number of intestinal microbial coding genomes is about 100 times the number of human coding genes, so it is also called the "second genome". [0003] Intestinal flora varies as high as 80% to 90% among individuals, mainly due to differences at the level of bacteria. In addition, the structure of intestinal flora will be affected by many factors, including host genetic factors, geographical conditions, eating habits, health status, drug ...

AI Technical Summary

Problems solved by technology

Biochips can only verify known species, obtain information on microbial diversity by detecting probes immobilized on the chip, and judge the abundance of microorganisms by the strength of the signal, but the results are inaccurate

Correlation analysis usually needs to follow the research ideas of Koch’s law, and return single or several microbial populations to animal models to prove the reliability of the correlation. There is a possibility that microorganisms are not easy to isolate and culture, and it is impossible to observe the influence of overall changes in the bacterial population on the model.

[0010] In addition, the above-mentioned research methods can only study one or several types of intestinal bacteria, or belong to correlation analysis, and cannot directly clarify the effect and mechanism of the joint action of various bacterial groups that actually occur in the body

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